(The server has completed structure and function predictions for 29534 proteins submitted by 6392 users from 82 countries)
(Number of visitors: 159225)

I-TASSER is an Internet service for protein structure and function predictions. Models are built based on multiple-threading alignments by LOMETS and iterative TASSER simulations. I-TASSER (as 'Zhang-Server') was ranked as the No 1 server in recent CASP7 and CASP8 experiments. The server is in active development with the goal to provide accurate structural and function predictions using state-of-the-art algorithms. The server is only for non-commercial use. Please send your questions and comments to zhanglab@ku.edu. (More about the server ...)

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If you need quicker responses, please submit your sequences to:

LOMETS (a multiple-threading server without structural refinement)
MUSTER (a single-threading server without structural refinement)

Copy and paste your sequence here (<1,500 residues): Or upload the sequence from your local computer: Email: (mandatory, where results will be sent to) ID: (optional, your given name of the protein) As an option, you can assign additional restraints & templates to guide I-TASSER modeling (More explanation on how to add restraints) Assign contact/distance restraints Atom-atom restraints; Explanation Specify template without alignment Type a PDBID; Explanation Specify template without alignment Upload a structure Explanation Specify template with alignment Template & alignment; Explanation

• 2009/01/10: Two new predictions were added in the output page:
  • Threading templates and the alignments which were identified by LOMETS and used by I-TASSER as initial input.
  • Structural homologs of I-TASSER which are identified by structural alignment program TM-align through the PDB library.
• 2009/03/16: Structure-based function predictions were developed based on the global and local match of the predicted 3D models with the known homolog and analog proteins. The function predictions include ligand binding site, enzyme commission, and gene ontology.
• 2009/07/20: Added features to allow users to assign external restraints to guide the I-TASSER structural modeling. The user-specified information includes:
  • Residue-residue contact/distance information
  • Specific template structures and alignments
  More details can be found at How to Add Restraints to I-TASSER Modeling.
• 2009/10/15: A new interface Search was added to allow users to search targets and predictions through the I-TASSER database.
• 2009/10/17: A timer was added to the Queue page to allow users track the implementation time of their targets. A number of other changes were made in the Queue page for improving the efficiency of retrieving I-TASSER output data.
• 2009/11/15: Molecular Dynamic simulations were implemented on I-TASSER models for removing steric clashes of side-chain atoms and for further refining rotamer conformations.