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I-TASSER results for job id S34192

Submitted Sequence

>TcBDF5
MEERRQKFYPEEELVALVRSLDRPQDEGLFSMDVLVVYPHLEQEYTRVCPKRCDLATAAE
KAANEAYSYDVNLTALREDIKLMVNNCYRFNGTKGPLANIAERFEAFAKEQIDAYVTKKA
GGRRLSSLRLSAVTATAAETSRGKRQRVESKDTSGVHGSQKPQPHRGGDKSRSGEMDGLS
ATSAEMVRLVDSLNRREDQGAFAVDVAEKYPELKKSYEEVCPVKMNLTILRERAASGYYL
GIPQPTTTSKNPEPQTQSKRRQLLASDASPSSVFGSTTAESLVLLRNDVELMVSNCVRFN
SNVQSWVELARSFQSFAHKKIDDFVIRHVPALKGTKSGVDAYVTGEKQQSQQQLLSQASK
SQQLHFTSTQDDEQMVTSTHSFASPSFSLLSTATATTYAMKGRAAEAASPPLRAPAVTVA
NYITPTIVPTALQPVFATPDALRRRLVSDHLHRETLRARLIRSSTRSHNDKGDNKNTINN
GTNNNKEEEEGKEEEEEEDGADKKEEKNSVQHTYNSAFSCRAVLCAFAASVREFYRLKRE
CQNFVNPFEYPRQEEQLYMDCIVLIEQQFERLFLHTLLYDKEKADFYDWNAEKAVRKIVG
SSEVTSLKEKDHEGFLCGFGSSWLDDAHLCYLVRFLQHLPQLMGLACATTVHLTGTELKD
ARPALQITEVAKGVIEKLARITEELLTFIAQYEQEAMNMESHAEIGKEVS

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 620 640 660 680 700 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MEERRQKFYPEEELVALVRSLDRPQDEGLFSMDVLVVYPHLEQEYTRVCPKRCDLATAAEKAANEAYSYDVNLTALREDIKLMVNNCYRFNGTKGPLANIAERFEAFAKEQIDAYVTKKAGGRRLSSLRLSAVTATAAETSRGKRQRVESKDTSGVHGSQKPQPHRGGDKSRSGEMDGLSATSAEMVRLVDSLNRREDQGAFAVDVAEKYPELKKSYEEVCPVKMNLTILRERAASGYYLGIPQPTTTSKNPEPQTQSKRRQLLASDASPSSVFGSTTAESLVLLRNDVELMVSNCVRFNSNVQSWVELARSFQSFAHKKIDDFVIRHVPALKGTKSGVDAYVTGEKQQSQQQLLSQASKSQQLHFTSTQDDEQMVTSTHSFASPSFSLLSTATATTYAMKGRAAEAASPPLRAPAVTVANYITPTIVPTALQPVFATPDALRRRLVSDHLHRETLRARLIRSSTRSHNDKGDNKNTINNGTNNNKEEEEGKEEEEEEDGADKKEEKNSVQHTYNSAFSCRAVLCAFAASVREFYRLKRECQNFVNPFEYPRQEEQLYMDCIVLIEQQFERLFLHTLLYDKEKADFYDWNAEKAVRKIVGSSEVTSLKEKDHEGFLCGFGSSWLDDAHLCYLVRFLQHLPQLMGLACATTVHLTGTELKDARPALQITEVAKGVIEKLARITEELLTFIAQYEQEAMNMESHAEIGKEVS
Prediction	CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHCCCCCCCCCCCSSCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCSSCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCSSSSHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCSSSCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCSSSCCCCCCCCCCCCSSSSCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCSSSSSSCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCSSSSSSSCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCSSSSHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
Conf.Score	97421355788999999986335676785026402026776764576278855769999998653545546778899889999841363079875799999999999865611666311687310201153233261113677543455567778877668777666653211344578924899999986631344588326547874817889999858985148999998756720167776645568661024555531456788742223189999999877599999763114655022999999888887513000024314100576555057870630002245662467683113676789712554054346887666556666763333455212598713651567510376026888886466978999888765653122100245311101334567644323478876770001357532235441221355233306332558999999999999998761454546632201576766557999999999999999998613146788899999999864554447786000247525788620021469999999998699887787711023554444567643655998789999999999999999999998745165776444579

Top 5 Models predicted by I-TASSER


Download Model 1	Download Model 2	Download Model 3	Download Model 4	Download Model 5
C-score= 0.03	C-score=-1.48	C-score=-3.19	C-score=-3.21	C-score=-3.21

Estimated accuracy of Model1: 0.72±0.11 (TM-score) 8.0±4.4Å (RMSD) (Read more about C-score of generated models)

Top 10 templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov.

Norm.
Z-score

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560                 580                 600                 620                 640                 660                 680                 700
                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |          

Sec.Str
Seq

CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHCCCCCCCCCCCSSCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCSSCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCSSSSHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCSSSCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCSSSCCCCCCCCCCCCSSSSCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCSSSSSSCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCSSSSSSSCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCSSSSHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
MEERRQKFYPEEELVALVRSLDRPQDEGLFSMDVLVVYPHLEQEYTRVCPKRCDLATAAEKAANEAYSYDVNLTALREDIKLMVNNCYRFNGTKGPLANIAERFEAFAKEQIDAYVTKKAGGRRLSSLRLSAVTATAAETSRGKRQRVESKDTSGVHGSQKPQPHRGGDKSRSGEMDGLSATSAEMVRLVDSLNRREDQGAFAVDVAEKYPELKKSYEEVCPVKMNLTILRERAASGYYLGIPQPTTTSKNPEPQTQSKRRQLLASDASPSSVFGSTTAESLVLLRNDVELMVSNCVRFNSNVQSWVELARSFQSFAHKKIDDFVIRHVPALKGTKSGVDAYVTGEKQQSQQQLLSQASKSQQLHFTSTQDDEQMVTSTHSFASPSFSLLSTATATTYAMKGRAAEAASPPLRAPAVTVANYITPTIVPTALQPVFATPDALRRRLVSDHLHRETLRARLIRSSTRSHNDKGDNKNTINNGTNNNKEEEEGKEEEEEEDGADKKEEKNSVQHTYNSAFSCRAVLCAFAASVREFYRLKRECQNFVNPFEYPRQEEQLYMDCIVLIEQQFERLFLHTLLYDKEKADFYDWNAEKAVRKIVGSSEVTSLKEKDHEGFLCGFGSSWLDDAHLCYLVRFLQHLPQLMGLACATTVHLTGTELKDARPALQITEVAKGVIEKLARITEELLTFIAQYEQEAMNMESHAEIGKEVS

1eqfA

0.20

0.08

0.35

3.25

Download

KSIHRRRTDPMVTLSSILESMRDLPNTYPFHTPVNKVVK----DYYKIITRPMDLQTLRENVRKRLYPS---REEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLE----------------------------KAINPLLD----------------DDDQVAFSFILDNIVTKMMAVPDSWPFHHPVKKFVPD----YYKVIVNPMDLETIRKNISKHKY---------------------------Q-------------SRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

1qgrA

0.09

0.19

0.99

1.99

Download

MEERAAVENLPTFLVELSRVLANPGNSQSLTSKDPDIKAQYQQRWLAIDAN--ARREVKNYVLHTLGTETYRPSSASQCVAGIACAEIPVNQWPELIPQLVANVTKESTLEAIGYICQDIDPEQLQDKSNEILTAIIQGMRKEEPSNNVKLAEFTKANFDKESERHFIMQVVCEATQCPDTRVRVAALYYQYMETYMGPALFAITIEAMKSDIDEVALQGIEFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKFYAKGALQYDDDDDWNPCKAAGVCLMLLATCCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGCSQLKPLVIQAMPTLIELMKDPSVVV----RDTAAWTVGRICELLPEAAINDVYLAPLLQCLIEGLSAEPRVASNVAYEAADVADDQEEPATYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQSTSDRIQFNDRKVQHQDALQISDVVMASLLRMFSGGVQEDALMAVSTLVEVLGGEFLKYMEAFKPFLGIGLKNGLVGDLCRALQSNIIPFCDEVMNLGNENVHRSLSVFGDIALAIGGEFKKYLEVVLNTLQQASQAQVDKSDYDMVDYLNELRESCLEAYTGIVQGLKGDQENVHPDVMLVQPRVEFILSFIDHIAGVVACAAGLIKDVLKLVEARPMIHELLTEGA

1eqfA

0.17

0.08

0.35

2.02

Download

RRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVV---KDYYKIITRPMDLQTLRENVRKRLYP---SREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDK-----------LARLEKAINPLLDDDDQVAFSF----------------ILDNIVTQKMMAVPDSWPFHHPVN---------------KKFVPD----YYKVIVNPMDLETIRKNISKHKY----------------------------------------QSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEY-------------------DEHLTQLEKDICTAKEAA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

1qbkB

0.08

0.20

0.91

1.92

Download

YEWKPD----EQGLQQILQLLKESQSTVQQKLEQLNQYPDFNNYLIFVLTKLKSEDEPTRSL---------SGLILKNNVKAHFQ-----NFPNGVTDFIKSECLNNIGDSSP--LIRATVGILITTIASKGELQNWPDLLPKLCSLLDSEDYNTCEGAFGALQKICEDSAEILDSDRPLNIIPKFLQFFKHSSPKIRSHAVA--------CVNQFIISRTQALLHIDSFTENLFALAGDEEPEVRKNVCRALVLLEVRMDRLLPHHNIVEYL-------------------------QRTQDQDENVALEACEFWLTLAEQPICKDVLVRHLPKLIPV--LVNGKYSDIDIILLKGDVEEDETIPDS--------EQDIRPRFHRSRTVAQQHDEDDDDDDEIDDDDTISDWNLRKCSAAALDVLANVYRDELLPHILPLLKELLFHHEWVVKESGILVLGAIAEGCQGIPYLPELIPHLIQCLSDKKASRYAHWVVSQPPDTYLKPLTELLKRILDSNKRVQEAACSAFATLEEEACTELFAFSKYQHKNLLIDAIGTLADSVGHHLECLSSVATALQSGFLPYCEPVYQRCVNLVQKTLAQALNNAQPDQYEAPDKDFIVALDLLSGLAEGLGGNIEQLVAWAIGEISIQGIEQPNRPNTPKTLLENTAITIGRLGYVCPQEVKILHGFKNQVFPLPLKERLAAFYG

1eqfA

0.15

0.08

0.35

3.17

Download

SIHRRRTDPLSSILESIINDMRDLPNTYPFHTPVNAKV--V-KDYYKIITRPMDLQTLRENVRKRLYP---SREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDD----------------------------------------------------------------D---------------DQVAFSFIL-----DNIVTQKMMADSWPFHHPVNKK--FVPDYYKVIVNPMDLETIRKNISKHKYQ--SRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

1u6gC

0.11

0.19

0.98

1.80

Download

ASYSSDKDFRFMATNDLMTELQKDSIKLDDDSPLVSKVKNMLSDKEQLLPSALAANVCKKGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCLLPQLTSPRLAVRKMSCFVDLIEHLLSELSKNDSMSTTRTVKFCNVDDDELKYLTYDMSWTRHEMLPISRFKEREENVKADVFHAYLSLLKRPVGETPLTMLQSQVPNIVKALHKQMKEKSVFNMLTELVNV--------LPGALTQHIPVLVPGIIFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQADPFYKITSEALLVTQQLVKPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNLGDNLGSDERLKNEITRLTTVKALTLIAGSPLKIDLRLRKNQRALKDILIKNYSDSLTAAMIDAVLDELPPLISESDMHAKVYPSSLSKNELIGLVRSPLLQLDFFQALVVTGTNNLGYMDLLRMLTGPVYSQTHKQSYYTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALLSLGEV--GHHIDLSGQLELKSVILEKSAASYALGSISVGNLPEYLPFVLQEITSQPKRQYLLLHSLKEIISSASVVGLKPYVENLKHCECAEEGTRNVVAECLGKLTLIDPEARSSVVTAVKFTISDHPQPEDPDLNVRRVALVTFNSAAHNKPSLIR--DLLDTVLPHLYNETKVRKELI

1eqfA

0.13

0.08

0.35

2.45

Download

RRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVN---AKVVKDYYKIITRPMDLQTLRENVRKRLYP---SREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPV-----NKKFVPDYYKVIVNPMDLETIRKNISKHKSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

1b3uA

0.09

0.18

0.76

1.76

Download

AAADGDDSL--YPIAVLIDELRNEDVQLRLNLGVERTRSELLPFLTDTIYDEDEVLLALAEQLGTFTTLVGGPEYVHCLLPPLES---LATVEETVVRDKAVESLRAISHEHSPSDLEAHFVPLVKRLA----------------------------------------------GGDWFTSRTSACGLFSVCYPRVS--------SAVKAELRQYFRNLCSDDTPASKLGEFAKVL-------------ELDNVKSEIIPMFSNLASDEQDSVRLLAVEACVNIAQLMPTLRQAAEDKSWRVRYMVAFTELQKAVGPEITKTDLVPAFQNLMKDC-------------EAEVRAAASHKVKEFCENLSADCRENVIM-------------------------------SQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQ-------------------------------------LKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAE------------DAKWRVRLAIIEYMPLLAGQLEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDP--NYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPLQSEVKPILEKLQDVDVKYFAQEALTVSLA

1eqfA

0.15

0.08

0.35

4.08

Download

-IHRRRTDPLSSILESIINDMRDLPNTYPFHTPVNAKV--V-KDYYKIITRPMDLQTLRENVRKRLYP---SREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDD------------------------------------------------------------------------------------QVAFSFILDNIVTQKMMAVSWPFHHPVNKK--FVPDYYKVIVNPMDLETIRKNISKHKYQ--SRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

1t7pA

0.12

0.17

0.87

0.77

Download

-------------MIVS-NALL--ESVTKFHCGVIYDYS--TAEYVSY--RPSDFGAYLDALEAEVARGGL----------IVFHNGHKY-----DVPALTKLAKLQLNREFH-----LPRENCIDTLVLSRLIH-----SNLKDTDMGLLRSGKLPGALEAWGYRLGEMKGEY--------KDDFKRMLEEQGEEYVDGM---EWWNFNEEMMDYNVQDV---VVTKALLEKLLSDKHYFPPEIDFTDVG----YTTFWSESL------------EAVDIEHRAAWLLAKQERNGFPFDT--KAIEELYVELAARRS-ELLRKLTETF--------GSWYQPKGGTEMFCHPRTGKPLPKYPRIKTPKEYVAGAPYTPVEHVVFNPSSRDHIQKKLQEAGWVPTKDKGAPVVDD--EVLEGVRVDDPEKQAAIDLIKEYLMIQKRIGQSAEGDKAWLGKIHGSVNPGAVTGRATHAFPNLAQPGVRSPYGEQCRAAEHHLDGITGKPWVQAGIDSGLELRCLAHFMARFDGEYAHEILNGDIHTKNQIALPTRDNAKTFIYGFLYGAGDEKIGQIVGAGKEKELKKKFLENPAIAALRESIQQT-LVEKWKRRWIKGLDGRKVHAALNTLLQSAGALICKLWIIKTEEMLVEKGLKHGWDGDFAYMAWV---QVG--CR-TEEIAQVVIETAQEAMRVDHWGKMGPICH

(a)	All the residues are colored in black; however, those residues in template which are identical to the residue in the query sequence are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade. (more about the colors used)
(b)	Rank of templates represents the top ten threading templates used by I-TASSER.
(c)	Ident1 is the percentage sequence identity of the templates in the threading aligned region with the query sequence.
(d)	Ident2 is the percentage sequence identity of the whole template chains with query sequence.
(e)	Cov. represents the coverage of the threading alignment and is equal to the number of aligned residues divided by the length of query protein.
(f)	Norm. Z-score is the normalized Z-score of the threading alignments. Alignment with a Normalized Z-score >1 mean a good alignment and vice versa.
(g)	Download Align. provides the 3D structure of the aligned regions of the threading templates.
(h)	The top 10 alignments reported above (in order of their ranking) are from the following threading programs:
	1: HHSEARCH 2: PROSPECT2 3: SP3 4: PROSPECT2 5: HHSEARCH I 6: PROSPECT2 7: FUGUE 8: PROSPECT2 9: HHSEARCH II 10: MUSTER

10 proteins in PDB which are structurally closest to the first I-TASSER model (identified by TM-align)

Rank	TM-score	RMSD^a	IDEN^a	Cov.	PDB Hit
1	0.9660	1.55	0.08	0.99	1qgrA Model1

2	0.7645	4.05	0.08	0.88	2bkuB Model1

3	0.7016	5.38	0.07	0.90	1wa5C Model1

4	0.5757	5.78	0.06	0.74	1qbkB Model1

5	0.5277	5.92	0.04	0.69	1u6gC Model1

6	0.5075	5.27	0.04	0.65	1qgtA Model1

7	0.4692	5.42	0.05	0.59	1b3uA Model1

8	0.4450	5.16	0.07	0.56	1w63A Model1

9	0.4254	5.04	0.06	0.53	1gw5A Model1

10	0.4143	7.10	0.05	0.59	2dq6A Model1

Structural alignment using TM-align
MELITILEKTVSPDRLELEAAQKFLERAAVENLPTFLVELSRVLANPGNSQVARVAAGLQIKNSLTSKDPDIKAQYQQRWLAIDAN--ARREVKNYVLHTLGTETYRPSSASQCVAGIACAEIPVNQWPELIPQLVANVTNPNSTEHMKESTLEAIGYICQDIDPEQLQDKSNEILTAIIQGMRKEEPSNNVKLAATNALLNSLEFTKANFDKESERHFIMQVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAITIEAMKSDIDEVALQGIEFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQDENDDDDDWNPCKAAGVCLMLLATCCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGCILEGPEPSQLKPLVIQAMPTLIELMKDPSVVVR----DTAAWTVGRICELLPEAAINDVYLAPLLQCLIEGLSAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPATYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLRMFSGGVQEDALMAVSTLVEVLGGEFLKYMEAFKPFLGIGLKNYAEYQVCLAAVGLVGDLCRALQSNIIPFCDEVMQLLLENLGNENVHRSVKPQILSVFGDIALAIGGEFKKYLEVVLNTLQQASQAQVDKSDYDMVDYLNELRESCLEAYTGIVQGLKGDQENVHPDVMLVQPRVEFILSFIDHIAGDEDHTDGVVACAAGLIGDLCTAFGKDVLKLVEARPMIHELLTEGRRSKTNKAKTLARWATKELRKLKNQA M----E-----------------ER--RQKFYPEEELVALVRSLDRPQDEG--LFS--M--DV--LV----VYPHLEQEYTRVCPKRCDLATAAEKAANEAYSYDVNLTALREDIKLMVNNCYRFNGTKGPLANIAERF----E----AFAKEQIDAYVTKKAGGRRLSSLRLSAVTATAAETSRGKRQRVESKDT--SG---V--H--GSQKPQPHRGGDKSRSGEMDGLSATSAEMVRL-V--DS------LNRREDQGAFAVDVAEKYPELKKSYEEVCPVKMNLTILRERAASGYYLGIPQPTTTSKNPEPQTQSKRRQL--LA-SDA-S----------P-SSVFGSTTAESLVLLRNDVELMVSNCVRFNSNVQSWVELARSFQSFAHKK-------IDDFVIRHVPALKGTKSGVDAYVTGEKQQSQQQLLSQASKSQQLHFTSTQDDEQMVTSTHSFASPSFSLLSTA---T--AT--TY--AM-KGRAAEAASPPLRAPAVTVANYITPTIVPTALQPVFATPDALRRRLVSDHLHRETLRARLIRSSTRSHNDKGDNKNTINNGTNNNKEEEEGK-EE-E---EE--E------DGADKKEEKNSVQHTYNSAFSCRAVLCAFAASVREFYRLKRECQNFVNPFEYPRQEE------Q--L-Y-M-DCIVLIEQQFERLFLHTLLYDK-EK---------ADFYDWNAEKAVRKIVGSSEVTSLKEKDHEGFLCGFGSSWLDDAHLCYLVRFLQHLPQLMGLACATTVHLTGTELKDARPALQITEVAKGVIEKLA----R---ITEELLTFIA--Q------YEQEAMNMESHAEIGKEV-S------------------------

MSTAEFAQLLENSILSPDQNIRLTSETQLKKLSNDNFLQFAGLSSQVLIDENTKLEGRILAALTLKNELVSKDSVKTQQFAQRWITQVSPEAKNQIKTNALTALVSIEPRIANAAAQLIAAIADIELPHGAWPELMKIMVDNTGAEQPENVKRASLLALGYMCE-S-ADALVSSSNNILIAIVQGAQSTETSKAVRLAALNALADSLIFIKNNMEREGERNYLMQVVCEATQAEDIEVQAAAFGCLCKIMSKYYTFMKPYMEQALYALTIATMKSPNDKVASMTVEFWSTICEEEIDIAYELAQFP----QSPLQSYNFALSSIKDVVPNLLNLLTRQNEDPEDDDWNVSMSAGACLQLFAQNCGNHILEPVLEFVEQNITADNWRNREAAVMAFGSIMDGPDKVQRTYYVHQALPSILNLMNDQSLQVK----ETTAWCIGRIADSVAESIDPQQHLPGVVQACLIGLQDHPKVATNCSWTIINLVEQLAEAT----P--SPIYNFYPALVDGLIGAANRIDNEF-NARASAFSALTTMVEYATDTVAETSASISTFVMDKLGQTMSVDENQLTLEDAQSLQELQSNILTVLAAVIRKSPSSVEPVADMLMGLFFRLLEKKDSAFIEDDVFYAISALAASLGKGFEKYLETFSPYLLKALNQVDSPVSITAVGFIADISNSLEEDFRRYSDAMMNVLAQMISN-PNA---RRELKPAVLSVFGDIASNIGADFIPYLNDIMALCVAAQNTKPE--NG-----------------TLEALDYQIKVLEAVLDAYVGIVAGLHDKPEALFPYVGT--IFQFIAQVAEDPQLYSEDATSRAAVGLIGDIAAMFPDGSIKQFYGQDWVIDYIKRTRSGQLFSQATKDTARWAREQQKRQLSL----------------------------------------- ----M--EE---------------R--RQKFYP---E-EELVALVRSLDRPQDEGL-FSM--DV--LV--VYP-HLE-QEYTRV--CPKRCDLATAAEKAANEAYSYDVNL-TALREDIKLMVNNCYRFNGTKGPLANIAERFE-------AFAKEQIDAYVTKKAGGRRLSSLRLSAVTATAAETSRGKRQRVESKDT--SG---V--HG--SQKPQPHRGGDKSRSGEMDGLSATSAEMVRL-V--D----S--LNRREDQGAFAVDVAEKYPELKKSYEEVCPVKMNLTILRERAASGYYLGIPQPTTTSKNPEPQTQSKRRQ-L-LA-SDA-S-----------PSSVFGSTTAESLVLLRNDVELMVSNCVRFNSNVQSWVELARSFQSFAHKK-------IDDFVIRHVPALKGTKSGVDAYVTGEKQQSQQQLLSQASKSQQLHFTSTQDDEQMVTSTHSFASPSFSLLSTA--TA--TT--YAMKGRAAEAASPPLRAPAVTVANYITPTIVPTALQPVFATPDALRRRLVSDHLHRETLRARLIRSSTRSHNDKGDNKNTINNGTNNNKEEEEGK-EE---E-EE-ED---G---AD-K--KEEKNSVQHTY-NSAFSCRAVLCAFAASVREFYRLKR--EC-QNFVNPFEYPRQEEQLYMDCIVLIEQ-QF------------ER-LFLHTLLYDKEKADFYDWNAEKAVRKIVG---------------SSEVTSLKEKDHEGFLCGFGSSWLDDAHLCYLVRFLQHLPQLMGLACATTVHL-T-------------------GTELKDARPALQITE----------------------------------------------------------------------VAKGVIEKLARITEELLTFIAQYEQEAMNMESHAEIGKEVS

MSDLETVAKFLAESVIASTAKTSERNLRQLETQDGFGLTLLHVIASTNLPLSTRLAGALFFKNFI-KRKWVDENGNHLLPANNVELIKKEIVPLMISLPNNLQVQIGEAISSIAD-SDFPDRWPTLLSDLASRLSNDDMVTNKGVLTVAHSIFKRWRPLFRSDELFLEIKLVLDVFTAPFLNLLKTVDEQITANEKASLNILFDVLLVLIKLYYDFNCQDIPEFFEDNIQVGMGIFHKYLSYSNPLLEHASVLIKVKSSIQELVQLYTTRYEDVFGPMINEFIQITWNLLTSISNQPKYDILVSKSLSFLTAVT--------------------RIPKYFEIFNNESAMNNITEQIILPNVTLREEDVELFEDDPIEYIRRDLEGTRRRACTDFLKELKEKNEVLVTNIFLAHMKGFVDQYMSNWKFKDLYIYLFTALAINGNITNAGVSSTNNLLNVVDFFTKEIAPDLTSNNIPHI-IL---RVDAIKYIYTFR-NQLTKAQLIELMPILAT-FLQ-TDEYVVYTYAAITIEKILTIRESNTSPAFIFHKEDISNSTEILLKNLIALILKHGSSPEKL-AENEFLMRSIFRVLQTSEDSIQPLFPQLLAQFIEIVTIMA--K--------NPSNPRFTHYTFESIGAILNYTQRQNLPLLVDSMMPTFLTVFSEDIQEFIPYVFQIIAFVVEQSATIPESIKPLAQPLLA-PNVWELKGNIPAVTRLLKSFIKTDSSIFPDLVPVLGIFQRLIA-SKAYEVHGFDLLEHIMLLIDMNRLRPYIKQIAVLLLQRLQNSK-T----------------------ER-YVKKLTVFFGLISNKLGSDFLIHFIDEVQDGLFQQIWGNFIITTLP-T-----IGNLLDRKIALIGVLNMVINGQFFQSKYPTLISSTMNSIIETAS--SQSIANLKNDYVEEISTFGSHFSKLVSISEKPFDPLPEIDVNNGVRLYVAEALNKYNAISGNTFLNTILPQLTQENQVKLNQLLV --------ME------------ERR-QKFYPEE--ELVALVRSLDRPQD-EG--LF-SMD-VLV-VYPHLEQEYTRVCPKRCDLATAAEKAANEAY-SYDVNLTALREDIKLMVNNCYRFNGTKGPLANIAERFE------AFAKEQIDAYVTKKA-GGR---RLSSLRLSAV-TAT--AAETSRGK--R-----------QRVESKD--TS-GV-H-GSQKPQPHRGGDKSRSGEMDG--L----------SATSAEMVRL--VD--SL-NR-RE--DQGAFAVDVAEKY-PE-LKKSYEEVCPVKMNLTILRERAASGYYLGIPQPTTTSKNPEPQTQS-KR----R--QL--LASDAS------------------------PSSVFGSTTAESLVLLRND-VELMVSNCVRFNSNVQ--SWVELARSFQSFAH-KK-----------I-D-DFVIRHVP-ALKGTK---SGVDAYVTGEKQQSQQQLLSQASKSQQLHFTSTQDDEQMVTSTHSFASPSFSLLST-ATA--TT-YAMK-GRAAE-AASPPLRAPAVTVANYITPTIV-PT-ALQP-VFATPDALRRRLVSDHLHRETLRARLIRSSTRSHNDKGDNKNTINNGTNNNKEEEEGKEE--EE--EE--D-------GADKKEEKNSVQHTYNSAFSCRAVLCAFAASVREFY-R--L---KRECQNFVNPFEYPRQEEQLYMDCIVLIE--Q---Q--F--ERLFLHTLLYDKEKADFYDWNAEKAVRKIV-----------G-SSEVTSLKEKDHEGFLCGFGSSWLDDAHLCYLVRFLQHLPQLMGLACATTVHL-TG-TE--LK--D-------AR-PALQITEVAKGVIEKLARITEELLTF-IA--Q--------------------YEQEAMNM--ESHAEIGKEVS----------------------------------------------------------------------------

YEWKPDEQGLQQILQLLKESQSPDTTIQRTVQQKLEQLNQYPDFNNYLIFVLTKLKSEDEPTRSLSGLILK-----NNVKAHFQNFPNGVTDFIKSECLNNIGDSSPLIRATVGILITTIASK-GEL--QNWPDLLPKLCSLLDSEDYNTCEGAFGALQKICEDSAEILD-SD---RPLNIIPKFLQFFKHS-SPKIRSHAVACVNQFIISRTQALLHIDSFTENLFALAGD-EEPEVRKNVCRALVLLEVRMDRLLPHHNIVEY-LQRTQDQDENVALEACEFWLTL--A---EQ---------------PICKDVLVRHLPKLIPVLVNGKYSDIDIILLKGDVEEDETIPDSEQDIRPRFHRSRTVAQQHDEDDDDDDEIDDDDTISDWNLRKCSAAALDVLANVYRDELLPHILPLLKELLFHHEWVVKESGILVLGAIAEGCQGIPYLPELIPHLIQCLSDKKALVR----SITCWTLSRYAH-WVVSQPPDTYLKPLTELLKRILDSNKRVQEAACSAFATLEEEA----CTELVPYLAYILDTLVFAFSK-YQ-H----KNLLILYDAIGTLADSVGHHLNKPEYIQLPPLIQKWNLKD--E-----------DKDLFPLLECLSSVATALQSGFLPYCEPVYQRCVNLVQKTLAQALNNAQPDQ-YEAPDKDFIVALDLLSGLAEGLGGNIEQLVARSNILTLYQ-CQ--D---K----PEVRQSSFAL-LGDLTKACFQHVKPCIAD-------------------------FPILGTNLN----------------------------------PEFISVCNNATWA---IGEISIQGIEQP--YIPVLHQLV--EIIN-------------------RPNTPKT-LLENTAITIGRLGYVCPQEVAPLQQFIRPWCTSLRNIRDNEEKDSAFRGICTISVNPSGVIQDFIFFCDAVASWINPKDDLRDFCKILHGFKNQVGDENWRRFSDQFPLPLKERLAAFYGV----------------------- ------------M--------------EERRQKFYPEEELVALVRSLDRPQD--------EGL--FSMDVLVVYPHLEQEYTRVCPKRCDLATAAEKAANEA--YSYDVNL-TALREDIKLMVNNCYRFNGTKGPLANIAE-RF--E-----------AFAKEQIDAYVTKKAGGRRLSSLRLSAVTATAAETSRGKRQRVESKD-TS-GVHGS--QKPQPHRGGDKSRSGEMDGLSATSAEMVRLVD-S--LNRRE-DQGAFAVDVAEKYPELKKSYEEVCPVKMNLTILRERAASGYYLGIPQPTTTSKNPEPQTQSKRRQL-LAS-DA-SP-----------------------------------------------------------SS-VFGSTTAESLVLLRNDVELMVSNCVRFNSNVQSWVELARSFQSFAHKK----IDDFVIRHVPALKGTKSGVDAYVTGEKQQSQQQLLSQASKSQQLHFTSTQDDEQMVTSTHSFASPSFSLLSTA--TA-TTYAMKGRAAEAASPPLRAPAVTVANYITPTIVPTALQPVFATPDALRRRLVSDHLHRETLRARLIRSSTRSHNDKGDNKNTINNGTNNNKEEEEGKEEE-EE-ED--G----------AD-KKEEKNSVQ-HT--YN--------SAFSCRAVLCAFAASVREFY----------R---LKRECQNFVNPFEYPRQEEQLYMDCIVLIE-QQ-----------------FERLFLHTLLYDKEKADFYDWNAEKAVRKIVGSSEVTSLKEKDHEGFLCGFGSSWLDDAHLCYLVRFLQHLPQLMGL----ACAT-----------TVHLTGTEL--KDA---RPALQITEVAKGVIEKLARITEEL--LT------------------------------------------------------------------------------------------------------------------------FIAQYEQEAMNMESHAEIGKEVS

-------------------------------------------------ASY----HISN--LLEKMTSSDKD-----FRF-MATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDKNGEVQNLAVKCLG---P-LVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCLLPQLTSPRLAVRKRTIIALGHLVMSC--------------------FVDLIEHLLSELSKNDSMSTTRTYIQCIAAISRQAGHRIGEYL-EKIIPLVVKFCNVDDDE-LREYCIQAFESFVRRCPKEVYPHVSTIINICLKYLTYDMSWK-VRRAAAKCLDAVVSTRHEMLPEF-YKTVSPALISRFKEREENVKADVFHAYLSLLKQTR-PVGE--TPLTMLQSQVPNIVKALHKQMKEKSV---KTRQCCFNMLTELVNVLPG-ALTQHIPVLVPGIIFSLND-----------KSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPF---YKITSEALLVTQQLVKVI-RPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQ--EVKERAISCMGQIICNLGDNLGSDLPNTLQ--IFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASFLR-------------KNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPP-------------------------------------------LI-SESDMHVSQMAISFLTTLAKVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGYMDLLRMLTGPVYSQTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALLSLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEITSQPKRQYLLLHSLKEIISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTLIDPETLLPRLKGYLISGSSYARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDLNVRRVALVTFNSAAHNKPSLIRDLLDTVLPHLYNETKVRKELIREVEMGPFKHTVDDGLDIRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLSTLCPSAVLQRLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVA----------------------------------------------ALLTIPEAEKSPLMSEFQSQISSNPELAA-- MEERRQKFYPEEELVALVRSLDRPQDEGLFSMDVLVVYPHLEQEYTRVCP--KRCDLA--TAAEKAANEAYSYDVNLTALREDIKLMVNNCYRFNGTKG---PL-ANIAERFE-AFA----KEQIDAYVTKKAGGRRLSSLRL-SAVTATAAETSR-GKRQRVES---KDTS--GV--HGSQK-PQPHRGGDKSRSGEMD-G-L-SA-TSAEMVRL-VD---S-LNRREDQGAFAVDVAEKYPELKKSYEEVCPVKMNLTILRERAASGYYLGIPQPTTTSKNPEPQTQSKRRQLL--A---SDASPSSVFGSTTAESLVLLRNDVELMVSNCVRFNSNVQSWVELARSFQSFAHKK----IDDFVIRHVPALKGTKSGVD-AYVTGEKQQSQQQLLSQASKSQQLHFTSTQDDEQMVTSTHSFASPSFSLLSTA--TA--TTYAMKGRA--AEAASPPLRAPAVTVANYITPTIVPTALQPVFATPDALRRRLVSDHLHRETLRARLIRSSTRSHNDKGDNKNTINNGTNNNKEEEEGKEEE-E-E-E--DG---A--D---K--KEEKNSVQHTYNSAFSCRAVLCAFAASVREFY--RL----------------KRECQNFVNPFEYPRQEEQLYMDCIVL-I-------------------EQQFERLFLHTLLY-----------------------------------DKEKADFYDWNAEKAVRKIVGSS-----------------------------EVTSLKEKDHEGFLCGFGSSWLDDAHLCYLVRFLQHLPQLMGLA-CATTVHLTGTEL--------------------KD-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ARPALQITEVAKGVIEKLARITEELLTFIAQYEQEAMNMESHAEIGK--E-------------------------VS

----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------M--DI-------------DPY--------------KEFGAT--VELLSFLP-----------------------SDF--FPSVRD---LLDTASALYREALESPEHC---SPHHTALRQAILCWGELMTLATWVGNNLEDPASRDLVVNYVNTNMGLKIRQL-LWFHISCLTFGRETVLEYLVSFGVWIRTPPAYRP---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PNAPILST--------------------------- MEERRQKFYPEEELVALVRSLDRPQDEGLFSMDVLVVYPHLEQEYTRVCPKRCDLATAAEKAANEAYSYDVNLTALREDIKLMVNNCYRFNGTKGPLANIAERFEAFAKEQIDAYVTKKAGGRRLSSLRLSAVTATAAETSRGKRQRVESKDTSGVHGSQKPQPHRGGDKSRSGEMDGLSATSAEMVRLVDSLNRREDQGAFAVDVAEKYPELKKSYEEVCPVKMNLTILRERAASGYYLGIPQPTTTSKNPEPQTQSKRRQLLASDASPSSVFGSTTAESLVLLRNDVELMVSNCVRFNSNVQSWVELARSFQSFAHKKIDDFVIRHVPALKGTKSGVDAYVTGEKQQSQQQLLSQASKSQQLHFTSTQDDEQMVTSTHSFASPSFSLLSTATATTYAMKGRAAEAASPPLRAPA-VTV-------ANYITPTIVPTALQPVFATPDALR--RRL-----VSDH------------------LHRETLRARLIRSSTRSHNDKGDNKNTINNGTN-NNKEEEEGKEE---E--EE--ED---------------------------------GADKKEEKNSVQHTYNSAFSCRAVLCAFAASVREFYRLKRECQNFVNPFEYPRQEEQLYMDCIVLIEQQFERLFLHTLLYDKEKADFYDWNAEKAVRKIVGSSEVTSLKEKDHEGFLCGFGSSWLDDAHLCYLVRFLQHLPQLMGLACATTVHLTGTELKDARPALQITEVAKGVIEKLARITE-------ELLTFIAQYEQEAMNMESHAEIGKEVS

AAADGDDSLY---------PIAVLIDEL-RN-----EDVQLRLNS-----IK-KLSTIALALGVERTRSELLPFLTDTIYD-EDEVLLALAEQLGTFTTLVGGPEYVHCLLPPL-ESLATVEETVVRDKAVESLRAISHEHSPSDLEAHFVPLVKRLAG-GDWFTSRTSACGLFSVCYPRVSSAVKAELRQYFRNLCSDDTPMVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLASDEQDSVRLLAVEACVNIA-QL-------------------LPQEDLEALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMSQILPCIKELVSDANQHVK----SALASVIMGLSPILGKDNTIE-HLLPLFLAQLKD-ECPE-VRLNIISNLDCVNEV------IGIRQLSQSLLPAIVELAEDAKWRVR------LA-IIE-YMPLLAGQLGVEFFDEKLNSLCMA-WLVDHVYAIREAATSNLK----------------KLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- ----------MEERRQKFYPEEELVALVRSLDRPQDEGLFSMDVLVVYPHLEQEYTRVCPKRCDLATAAEKAANEAYSYDVNLTALREDIKLMVNNCYRFNGTKGPLANIAERFEAFAKEQ----IDAYVTKKAGGRRLSSL--RL-SAVTATAAETSRGKRQRVESKDT--SG-VHGSQK-PQPHRGGDKSRSGEMDGLSATSAEMVR-LV--DS---LNRREDQGAFAVDVAEKYPELKKSYEEVCPVKMNLTILRERAASGYYLGIPQPTTTSKNPEPQTQ-SKRRQL--LA-S-DASPSSVFGSTTAESLVL-LRNDVELMVSNCVRFNSNVQSWVELARSFQSFAHKK-------IDDFVIR-HVPALKGTKSGVDAYVTGEKQQSQQQLLSQASKSQQLHFTSTQDDEQMVTSTHSFASPSFSLLSTA--TA-TTYAMKGRAAEAASPPLRAPAVTVANYITPT-IVPT-ALQPVFATPDALRRRLVSDHLHRETLRARLIRSSTR-SHN-----DKGDNKNT---INNGTNNNKEEEEGKEEEEEEDGADKK---------------------------------------------------------------------------------------------------------------EEKNSVQHTYNSAFSCRAVLCAFAASVREFYRLKRECQNFVNPFEYPRQEEQLYMDCIVLIEQQFERLFLHTLLYDKEKADFYDWNAEKAVRKIVGSSEVTSLKEKDHEGFLCGFGSSWLDDAHLCYLVRFLQHLPQLMGLACATTVHLTGTELKDARPALQITEVAKGVIEKLARITEELLTFIAQYEQEAMNMESHAEIGKEVS

MPAPIRLREL--------------------------------------------------------------------------------------------------------------------------------------------------IRTIRTARTQA---------EEREMIQK-E-----------C-------AAIRSSFREEDNTYRCR---NVAKLLYMHMLGY-PA----------------------HFGQLECLKLIASQKFTDKRIGYLGAMLLLDER-QDVHLLMTNCIKNDLNHSTQFVQGLALCTLGCMGSSEMCRDLAGEVEKLLKT-SNS-Y-LR----KKAALCAVHVIRKVPELMEM--FLPATKNL-LNE-KNHGVLHTSVVLLTEMCERS-----PDMLAHFRKLVPQLVRILKNLIMSGYSPEHDVSGISDPFLQVRILRLLRILGRNDDDSSEAMNDILAQVATNT--ET--S-----KNVGNAILYETVLTIMDIKSESGLRVLAINILGRFLLNNDKNIRYVALTSLLKTVQTDH-NA--VQ-RHRSTIVDCLKDLDVSIKRRAMELSFALVNGNNIRGMMKELLYFLDSCEPEFKADCASGIFLAAEKYAPSKRWHIDTIMRVLTTAGS-Y-----------------------------V--RDDAVPNLIQLIT------NSVEMHAYTVQRLYKAILGDYSQ--QPLV-QVAAWCIGEYGDLLVSGQCEEEEPIQVTEDEVLDILESVLISNMS---TSVTRGYALTAIMKLSTRFTCTVNRIKKVVSIYGSSIDVELQQRAVEYNALFKKYDHMRSALLERMPVMEK---------------------------------------- ----------MEERRQKFYPEEELVALVRSLDRPQDEGLFSMDVLVVYPHLEQEYTRVCPKRCDLATAAEKAANEAYSYDVNLTALREDIKLMVNNCYRFNGTKGPLANIAERFEAFAKEQIDAYVTKKAGGRRLSSLRLSAVTATAAETSRGKRQRVESKDTSGVHGSQKPQPHRGGDKSRSGEMDGLSATSAEMVRLVDSLNRREDQGAFAVDVAEKYPELKKSYEEVCPVKMNLTILRERAASGYYLGIPQPTTTSKNPEPQTQSKRRQLLAS-DASPSSVFGSTTAESLVLLRNDVELMVSNCVRFNSNVQSWVELARSFQSFA-HKK--IDDFVIRHVPALKGTKSGVDAYVTGEKQQSQQQLLSQASKSQQLHFTSTQDDEQMVTSTHSFASPSFSLLSTAT-A-TTYAMKGRAAEAASPPLRAPAVTVANYITPTIV-P-TALQPVF---ATPDALRRRLVSDHLHRETLRARLIRSSTRSHNDKGDNKNTINNGTNNNKEEEEGKEEE--EE-ED---G-ADKKEEKNSVQHTY-NSAF--SCRAVLCAFAASVREFYRLKRECQNFVNPFEYPRQEEQ---L---YM-DCIVLIEQQFER-LFLHTLLYDKEKADFYDWNAEKAVRKIVG--------S---SEVTSLKEKDHEGFLCGFGSSWLDDAHLCYLVRFLQHLPQLMGLACATTVH------LTGTELK----------------------DARPA-LQ--------------------------------------------ITEV----------------------------------------------------------------------AKGVIEKLARITEELLTFIAQYEQEAMNMESHAEIGKEVS

GMRGLAV-----------------------------------------------------------------------FIS--------------------------------DIRNCKSKEAE--------------IK-RINKELAN--------------------------IRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDID--F---GHMEAVNLLSSNRYTE-KQIGYLFISVLVN--S--------------------NSELIRLINNAIKNDLASRNPTFMGLALHCIANVGSREMAEAFAGEIPKILV--AGDTMDSVKQSAALCLLRLYRTSPDLVPMG--DWTSRVVHLLNDQHLGVV----TAATSLITTLAQKNPEEFK--TSVSLAVSRLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCY----------PPPEDPAVRGRLTECLETILNKAQEPPKSKKVQHSNAKNAVLFEAISLIIHHD--S--E-PNLLVRACNQLGQFLQ-H--R-----ETNLRYLALESMCTLASSEFSHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAQQIVAEMLSYLETADYSIREEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGD------------Y--VS--EEVWYRVIQIVINRDDVQGYAAKTVFEALQAPACHENLVKVGGYILGEFG----------------------------------------------------------------------------------------------------------- -------MEERRQKFYPEEELVALVRSLDRPQDEGLFSMDVLVVYPHLEQEYTRVCPKRCDLATAAEKAANEAYSYDVNLTALREDIKLMVNNCYRFNGTKGPLANIAERFEAFAKEQIDAYVTKKAGGRRLSSLRLSAVTATAAETSRGKRQRVESKDTSGVHGSQKPQPHRGGDKSRSGE---M-DGLSATSAEMVRL-V----DSLNRREDQGAFAVDVAEKY-PELKKSYEEVCPVKMNLTILRERAASGYYLGIPQPTTTSKNPEPQTQSKRRQL--LA---SDASPSSVFGSTTAESLVLLRNDVELMVSNCVRFNSNVQSWVELARSFQSFAHKK------IDDFVIRHVPALKGTKSGVDAYVTGEKQQSQQQLLSQASKSQQLHFTSTQDDEQMVTSTHSFAS--------------PSFSLLSTA-T-ATTYAMKGRAAEAASPPLRA-PAVTVANYITPTI-VP----TA--LQPVFATPDALRRRLVSDHLHRETLRARLIRSSTRSHNDKGDNKNTINNGTNNNKEEEEGKEE--EE--EED---GADKKEEKNSVQHTYN-SAFSCRAVLCAFAASVREFYR-LKRECQNFVNPFEYPRQEEQLYMDCIVLIEQQF------------ER-LFLHTLLYDKEKADFYDWNAEKAVRKIVGSSE--------------------------------------------VTSLKEKDHEGFLCGFGSSWLDDAHLCYLVRFLQHLPQLMGLACATTVHLTGTELKDARPALQITEVAKGVIEKLARITEELLTFIAQYEQEAMNMESHAEIGKEVS

QAKYRHDYRAPDYQITDIDLTF--------DLDAQKTVVTAVSQAVRHGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAANTALEGLYQSGD-------------------------ALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGNRV--AQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRS-----GREVALELYVDRGNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAME-NKGLNIFNSKYVLARTDTATDKDYLDIERVIGHEYFH------------------------NWT-GNRVTCRDWFQLSLKEGLTVFRDQEFSSDLGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIHTLLGEENFQK-------------------------------------------------------GMQLYFERHDGSAATCD--------DF--VQ-AME-D---ASNV-DL-SHFRRWYSQSGTPIVTVKDDYNPETEQYTLTISQRTPATPDQ-----A--EK-Q-PL--H-------------IPF-AIELYDNEGKV------------------IPLQKGGH--PV-N-SVLNVTQAEQ-TF-VFDNVYFQPV-----------PALLCEFSAPVKLEYK--WS----DQ-QLTFLMRHA-RNDFSRWDAAQSLLATYIKLNVARHQQGQPLSL--PVHVADAFRAVLLD-EKIDP---ALAAEILTLPSVNEMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNAN--YQSEYRVEHEDIAKRTLRNACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQ--L---P-CRDALMQEYDDKWHQNGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPA-AFHAEDGSGYLFL-----------VE-MLTDLNSRNP-------Q-VASRLIEPLIRLKRYDAKRQE-----------KMRAALE--------------QLKGLENLSGDLYEKI---------------------T-KALA ----------------------MEERRQKFYP-------------------------------------------------------------------------------------EEELVALVRSLDRPQDEGLFSMDVLVVYPHL----E--Q-------------EYTRVC---------------PKRCD------LATAAEKAAN------------------------EAYSYDV------------------------------------NLTALRED-------------IKLMVNN-------------------------------CYRFNGTKGPLANIAERFEAFAKEQI-DAYVTKKAGGR----------------------------------------------------------------------------------------RLSSLRLSAVTATAAETSRGKRQRVESKDTSGVHGSQKPQPHRGGDKSRSGEMDGLSATSAEMVR---LVDSLNRREDQGA-FAVDVAEKYPELKKSYEEVCPVKMNLTILRERA----------------------ASGYYLGIPQPTTTSKNPEPQTQSKRRQLLASDASPSSVFGSTTA---------ESLVLLRNDVELMVSNCVRFNSNVQSWVELARSFQSFAHKKIDDFVIRHVPALKGTKSGVDAYVTGEKQQSQQQLLSQASKSQQLHFTSTQDDEQMVTSTHSFASPSFSLLSTAT-AT--TY-AMK-GR--AAEAASPPLRAPAVTVANYITPTIVPTALQPVFATPDALRRRL--VS-----------D-HLHRETLRARLIRSSTRSHNDKGDNKNTINNGTNNNKEEEEGKE-EE--EE-EDGAD-KKEEKNSVQHT-YNSAFSCRAVLCAFAASVREFYRLKRECQNFVNPFEYPRQ-E--E-QL--Y-MDCIVLIEQQFERLFLHTLLYDK--E-KADFYDWNAEK--A--VRK-----------IVGSSEVTSLKEKDHEGFLCGFGSSWLDDAHLCYLVRFLQHLPQ----------LMGLACATTVHL------TGTELKDARPALQITEVAKGVIEKLARITEELLTFIAQYEQEAMNMESHAEIGKEVS

(a)	Residue pairs that are structurally aligned by TM-align program are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade.(more about the colors used)
(b)	Ranking of proteins is based on TM-score of the structural alignment between Model1 and the PDB structures in our template library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.

Function Prediction

Predicted EC Numbers

Rank	TM-score	RMSD^a	IDEN^a	Cov.	EC-Score	PDB Hit	EC No.
1	0.4161	7.91	0.06	0.64	0.4570	1ofeB	1.4.7.1

2	0.4160	7.16	0.05	0.60	0.4459	3b3bA	3.4.11.2

3	0.4022	8.09	0.04	0.63	0.4112	2vdcF	1.4.1.13

4	0.3472	8.23	0.05	0.55	0.3744	1t3tA	6.3.5.3

5	0.3497	6.27	0.05	0.47	0.3716	1dceA	2.5.1.60

(a)	Ranking is based on EC-score, which is a confidence score for the Enzyme Classification (EC) Number prediction.
(b)	RMSD^a is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)	IDEN^a is percentage sequence identity in the structurally aligned region.
(d)	Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)	EC-Score is defined based on the C-score of the structure prediction and similarity of the model with known enzyme structures, as identified using both global and local structural alignment programs. The global similarity score uses TM-score, IDEN^a,RMSD^a and Cov. of the structural alignment by TM-align, while the local match compares the structural and chemical similarity of local spatial motifs in the model with known catalytic site of enzymes. A prediction with a EC-score >1.1 signifies a prediction with high confidence (upto 3 digit numbers of EC) and vice versa (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).

Predicted GO terms

Rank	TMscore	RMSD^a	IDEN^a	Cov.	PDB Hit	Fh-Score	Associated GO Terms
1	0.8791	3.16	0.09	0.97	2bptA	1.07	GO:0005515 GO:0008320 GO:0008565 GO:0000059 GO:0006612 GO:0010926 GO:0022607 GO:0034504 GO:0034621 GO:0034622 GO:0043623 GO:0051170 GO:0051292 GO:0051641 GO:0051649 GO:0070271 GO:0005622 GO:0005623 GO:0005634 GO:0005643 GO:0005737 GO:0012505 GO:0016020 GO:0016021 GO:0031975 GO:0043229 GO:0043234 GO:0044428 GO:0046930
2	0.8141	4.01	0.07	0.93	1uklB	0.92	GO:0008565 GO:0019904 GO:0000059 GO:0006605 GO:0006610 GO:0010926 GO:0022607 GO:0034504 GO:0034621 GO:0034622 GO:0043623 GO:0051170 GO:0051641 GO:0051649 GO:0070271 GO:0005622 GO:0005623 GO:0005634 GO:0005643 GO:0005737 GO:0012505 GO:0016020 GO:0016021 GO:0031975 GO:0043229 GO:0043234 GO:0044428 GO:0046930
3	0.6739	3.90	0.07	0.76	2qnaA	0.76	GO:0008139 GO:0008270 GO:0008565 GO:0019904 GO:0043169 GO:0000059 GO:0000060 GO:0006605 GO:0006607 GO:0006610 GO:0010926 GO:0022607 GO:0034504 GO:0034621 GO:0034622 GO:0043623 GO:0044419 GO:0051170 GO:0051641 GO:0051649 GO:0070271 GO:0005622 GO:0005623 GO:0005634 GO:0005643 GO:0005654 GO:0005737 GO:0005829 GO:0012505 GO:0016020 GO:0016021 GO:0031974 GO:0031975 GO:0043229 GO:0043234 GO:0044428 GO:0046930
4	0.6093	5.55	0.06	0.76	2z5oA	0.66	GO:0005515 GO:0008139 GO:0008565 GO:0000059 GO:0000060 GO:0006605 GO:0010926 GO:0022607 GO:0034504 GO:0034621 GO:0034622 GO:0043623 GO:0044419 GO:0051170 GO:0051641 GO:0051649 GO:0070271 GO:0005622 GO:0005623 GO:0005634 GO:0005643 GO:0005737 GO:0012505 GO:0016020 GO:0016021 GO:0031975 GO:0043229 GO:0043234 GO:0044428 GO:0046930
5	0.5874	5.89	0.06	0.78	1z3hB	0.64	GO:0005049 GO:0008262 GO:0000059 GO:0000087 GO:0000278 GO:0006611 GO:0007067 GO:0008283 GO:0010926 GO:0022607 GO:0034504 GO:0034621 GO:0034622 GO:0043623 GO:0051168 GO:0051170 GO:0051301 GO:0051641 GO:0051649 GO:0070271 GO:0005622 GO:0005623 GO:0005634 GO:0005643 GO:0005737 GO:0012505 GO:0016020 GO:0016021 GO:0031975 GO:0043229 GO:0043234 GO:0044428 GO:0046930
6	0.4787	5.14	0.06	0.60	2pkgB	0.53	GO:0003823 GO:0008601 GO:0046982 GO:0009653 GO:0009987 GO:0010926 GO:0022610 GO:0040008 GO:0044237 GO:0048869 GO:0050789 GO:0050896 GO:0065007 GO:0000159 GO:0005622 GO:0005623 GO:0005625 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0015630 GO:0016020 GO:0043229
7	0.4168	7.10	0.06	0.61	3ciyB	0.47	GO:0003725 GO:0004888 GO:0005515 GO:0001816 GO:0002376 GO:0006955 GO:0009607 GO:0009987 GO:0044237 GO:0050789 GO:0051704 GO:0065007 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005783 GO:0005789 GO:0010008 GO:0012505 GO:0016020 GO:0016021 GO:0042175 GO:0043229 GO:0044432 GO:0044440 GO:0044444
8	0.4481	5.31	0.04	0.56	2pf4D	0.46	GO:0005515 GO:0000159 GO:0005622 GO:0005623 GO:0005737 GO:0005829
9	0.4161	7.91	0.06	0.64	1ofeB	0.46	GO:0005506 GO:0005515 GO:0015930 GO:0016041 GO:0043169 GO:0051538 GO:0006536 GO:0006537 GO:0006541 GO:0008652 GO:0009058 GO:0009308 GO:0009309 GO:0009987 GO:0019752 GO:0044249 GO:0055114
10	0.4286	5.45	0.05	0.55	2vglA	0.45	GO:0005515 GO:0008289 GO:0008565 GO:0006886 GO:0006897 GO:0010324 GO:0034613 GO:0046907 GO:0051641 GO:0051649 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005905 GO:0030119 GO:0030131 GO:0044444 GO:0048475

Consensus Prediction of Gene Ontology terms

Molecular Function    Biological Process Cellular Location

GO term GO-Score    GO term GO-Score GO term GO-Score

GO:0005515 0.642    GO:0009987 0.640    GO:0005623 0.641

GO:0022892 0.450    GO:0010926 0.458    GO:0005737 0.596

GO:0005215 0.450    GO:0015031 0.450    GO:0005622 0.596

GO:0008565 0.450    GO:0051234 0.450    GO:0016020 0.595

GO:0019904 0.168    GO:0051649 0.450    GO:0043234 0.549

GO:0005048 0.142    GO:0033036 0.450    GO:0043229 0.505

GO:0042277 0.142    GO:0008104 0.450    GO:0043231 0.505

GO:0008139 0.142    GO:0006810 0.450    GO:0044425 0.497

GO:0043169 0.121    GO:0046907 0.450    GO:0005634 0.458

GO:0043167 0.121    GO:0016043 0.450    GO:0031224 0.452

(a) Ranking in the first table is based on a function prediction score (Fh-score), which is calculated based on the C-score of the structure prediction and the TM-score, IDEN^a, RMSD^a and Cov. of the structural alignment by TM-align between the predicted model and the PDB structures (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).

(b) RMSD^a is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.

(c) IDEN^a is the percentage sequence identity in the structurally aligned region.

(d) Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.

(e) A consensus prediction of GO terms is derived from the structural analogs that have an Fh-score of >=1.0. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on the Fh-score of the template from which the GO term is derived. A prediction with a GO-score >0.5 signifies a prediction with high confidence and vice versa.

Predicted Binding Site

No binding site residues were found in the predicted structure.

	Please cite following articles when you use the I-TASSER server:
1.	Yang Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9:40 (2008).
2.	Yang Zhang. Template-based modeling and free modeling by I-TASSER in CASP7. Proteins, 8: 108-117 (2007).
3.	Sitao Wu, Jeffrey Skolnick, Yang Zhang. Ab initio modeling of small proteins by iterative TASSER simulations. BMC Biology, 5:17 (2007).