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I-TASSER results for job id S34194

  Submitted Sequence

>osaws
MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNA
VSSEETNDFKQETLPSKSHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDES
HHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPD
ATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQS
RLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRIS
HELDSASSEVN

  Predicted Secondary Structure

Sequence                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300
                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |            
MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN
PredictionCCSSCHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCSSSSCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHCHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCSSSSCCCCCCCCCCCCC
Conf.Score94420699998888655243346777065414455875000036788788765305421002211353011137775668855454455565555545677864221235777655654456665565102278777641223677887778888753222102035664136556688874156777765543246543231465448987433455553011156643212233344222055468863000233123000022101244010014566157875323442335640433355433569

  Top 5 Models predicted by I-TASSER

Download Model 1 Download Model 2 Download Model 3 Download Model 4 Download Model 5
C-score=-2.22
 C-score=-4.13
 C-score=-5
 C-score=-2.93
 C-score=-5
 
Estimated accuracy of Model1: 0.45±0.15 (TM-score)    11.5±4.5Å (RMSD)    (Read more about C-score of generated models)

  Top 10 templates used by I-TASSER

RankPDB
Hit		Iden1Iden2Cov.Norm.
Z-score		Download
Align.		                   20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300
                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |           
Sec.Str
Seq		CCSSCHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCSSSSCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHCHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCSSSSCCCCCCCCCCCCC
MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN
11dgiR 0.12 0.15 0.90 0.73Download VVVQA-----------PTQVPGFLGDS-YLQVPNMEVTHVSTWARHGESGSMAVFHQTQGPSYSESKRLEFVAARLGAELRN-RMFGLRVEDEGN------------VTFPQGSRSVDIWLRVLAKPQNTAEVQKVQLTGE--PVPMARCVSTGGRPPAQITWHSDLGGMPNTSQVPGFLSGTVTVTSLWILVPSSQV-----DGKNVTCKVEHESFEKPQLLTVNLTVYYPPEVSISGYDNNWYLGQNEATLTCDARSNPEPTGYNWSTTMGPLPPFGAQLLIRPVDKPINTTLICNVTNALGARQAELT
23c9hA 0.14 0.17 0.72 0.70Download --------------PALAQFPALSG----QTLVSANPDIAVHYEDMLTGIYDRIVK----------------ETDAG-KKTADFA----FSSAMDLQVKLSNDGYAQ---RSD---------LAM--------SARWPAWANW--RNTAYALT-FEPAVFVYH----KPSFT---TEKPPATRAEFVDYLERHAKE--VHG-----RIATYDIER-GVGFSRDQEQF----GDI--WSVIKA--MGAAGVKVYSTRIGAA-ANPELGRRYLEKEGQTIQLQIAVSPEV-AGENTLRVPVSPGLDQVKRSR-
31ji1A 0.13 0.14 0.89 0.45Download QKLGYIKK-TLGANILNPIFKAPT--------NHKYDTQDYMAVDPDNSTLQTLINDIHSTANGPKGYLILDGVFNHTGDSHPWFDKYNNFSSQGAYESQSSPWYNYYTFYTWPDSYASFLGFNSLPKLNYGNSGSAVRGVIYNNSNSVAKLNPPYSD----GWRDAAQYVDANGNNGSDVTNHQIWSEFRNAVKGVNSNAAIIGEYWGNANPWTAQG------NQWDAATNFDGFTQPVSEWITGKDYQNNSAS--ISTTQFDSWLRGTRANYPTNVQQSM------------MNFLSNHDITRFATRSG
42eyzA 0.08 0.14 0.91 1.59Download M----------------------------AGNFDSEERSSWYWGRLSRQEAVALLQGQRHLVRDSSTSPGDYVLSVSENSRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRIGDQEFDSLPALLEFYKIHYLDTTTLIEPVSRSRQGSGVILRQEEAEYVRALFDFNGNDEEDLPFKKGDILRIRDKPEEEDSEGKRGMIPVPYVEKYRPASASVSALIGGNQEGSHPQPLGGPEPGPYAQPSVNTPLPNLQNGPIYARVIQKRVPNAYDKTALALEVGELVKVTKINVSGQWEGECNLDQQNPEEDFS
52bsgA 0.12 0.15 0.98 0.86Download SRISWI---KNGEEILGADTQYGSEGSMNRPTVSVLRNVEVLDKNIG-ILKTSLETANSDIKTIQINKKDISDLKTLTSEHTEILNGTNNTVDSILADIIRNDNNTDVIEVGNLREELVYSRLNEIDTKQTTVESDISAIKTSISIITSVNTNTDNIASINLEIKQRLTVIETSIIKGQIKDN---TTSILRANVSWLNQIVSLLNRVSTIETSVSGLNNAVQNLQVEIGNNSAGIKGQVVALNTLVNGTNPNGSTVEERGLTNSIKANETNIASVTQEVNTAKGNISSLQGDVQALQEAGYIPEAPRDGQ
62bykA 0.14 0.04 0.21 0.78Download GLITNEVLFLMTKCTELFVRHLAGAAYTEEFGQRPGE----------ALKYEHL---SQVVNKNKNLEFLLQIVPQK------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
71an9A 0.09 0.13 0.79 0.53Download MRVVVIGAGVIGLSTALCIHERYH---------SVLQPLDVKVYADRFTPFTTTDVAAGLWQPYTSEP---------------------------------------------SNPQEANWNQQTFNYLLSHIGSPNAANMGLTPVSGYNLFREAVPDPYWKDMVLGFRKLTPRELDMFPDYRYGWFNTSLILEGRKYLQWLTERLTERGVKFFLRKVESFEEVARGGADVIINCTGVWAGVLQPDPLQVRLEREQLRFGSSNTEVIHNYGHGGYGLTIHWGCALEVAKLFGKVLEERNLL----------
82bykA 0.21 0.04 0.06 1.03Download ----------------------------------------------------------QVVNKNKNLEFLLQIVPQK------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
92d7iA 0.13 0.18 0.96 0.30Download EGWSSLLRTVHSVLNRSPPELVDDFSDREHLKYMALFPSVRILRTK---KREGLIRTRMLGASVATGDVIT-FLDSNVNWLPPLLDRIARNRKTIVCPMIDVIDHDDFRYETQAGDAMRGAFDWEMYYKRIPIPPELQKADPSDPFSPVM-----AGGLFAVD-RKWFWELGGYDPGLEIWGGEQYEISFKVWMCGGRMEDIPCSRVIYRKYVPYKVAGVSLARNLKR--VAEVWMDEYAEYIYQRRPEYRHLSAGDVAVQKKLRSSLNCKSKWFMTKWDLPKFYPPVEPPAAAIRNVGTGCADTKHGALG
101atuA 0.11 0.15 0.96 0.69Download AEFAFILFSPVSIAAAF-LSLGAKGDTHDEILEGLNFNLTEIPEAQIHEGFQELLRTLNQPDSQLQLTTGNGLFLSEGLKLVDKFLEDVKKLYHSEAFTVNFGDTEEAKKQINDVDLVKELDRDTVFALVNYIFFKGKWERPFEVKDTEEEDHVDQVTTVKVPMMKRLGMFNIQHCKKLSSWLGNATA-PDEGKLQHLENELT-HDIITKFLENEDRRS-SITGTDLKSVLGQLGITK----FSNGADSGVTEEAPLKLSKAVHKAVLTI--DEKGTEAAGAMFLEAIPMSIPP-EVKFNKPIEQNTKAPN
(a)All the residues are colored in black; however, those residues in template which are identical to the residue in the query sequence are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade. (more about the colors used)
(b)Rank of templates represents the top ten threading templates used by I-TASSER.
(c)Ident1 is the percentage sequence identity of the templates in the threading aligned region with the query sequence.
(d)Ident2 is the percentage sequence identity of the whole template chains with query sequence.
(e)Cov. represents the coverage of the threading alignment and is equal to the number of aligned residues divided by the length of query protein.
(f)Norm. Z-score is the normalized Z-score of the threading alignments. Alignment with a Normalized Z-score >1 mean a good alignment and vice versa.
(g)Download Align. provides the 3D structure of the aligned regions of the threading templates.
(h)The top 10 alignments reported above (in order of their ranking) are from the following threading programs:
       1: MUSTER   2: HHSEARCH   3: SP3   4: PROSPECT2   5: PPA-I   6: HHSEARCH I   7: FUGUE   8: HHSEARCH II   9: SPARKS   10: MUSTER   

  10 proteins in PDB which are structurally closest to the first I-TASSER model (identified by TM-align)

RankTM-scoreRMSDaIDENaCov.PDB
Hit
10.6269 4.80 0.04 0.851w0rA
Model1
 
20.3421 5.32 0.05 0.501xf1A
Model1
 
30.3170 5.89 0.02 0.503ecqA
Model1
 
40.3140 6.48 0.01 0.533fcsA
Model1
 
50.3101 5.77 0.02 0.492uv9A
Model1
 
60.2951 6.99 0.04 0.531uf2A
Model1
 
70.2856 6.38 0.03 0.481t3tA
Model1
 
80.2807 5.98 0.03 0.453dmkA
Model1
 
90.2799 6.68 0.04 0.493f8uA
Model1
 
100.2774 6.44 0.05 0.471mhsA
Model1
 
Structural alignment using TM-align
 
DPVLCFTQYEESSGKCKGLLGGGVSVEDCCLNTAFAYQKRSGGLCQPCRSPRWSLWSTWAPC--SVTCSEGSQL--RY--RRCVGWNGQCS---GKV--APGTLEWQ-LQACEDQQC---CPEMGGWSGWGPWEPCSVTCSKGTRTRRRACNHPAP-KCGGHCPGQAQESEACDTQQVCPTHGAWATWGPWTPCSASCHGGPHEPKETRSRKCSAPEPSQKPPGKPCPGLAYEQRRCTGLPPCPVAGGWGPWGPVSPCPVTCGLGQTMEQRTCNHPVPQHGGPFCAGDATRTHICNTAVPCPVDGEWDSWGEWSPCIRR--N-M--KSISCQEIPGQQSRGRTCRGRKF-----DGHRCA-------GQQQDIRHCYSIQHCPLKGSWSEWSTWGLCMPPCGPNPTRARQRLCTPLLPKYPPTVSM----VEGQGEKN-VTFWGR--------PLPRCEELQGQKLVVEEKRPCLHVPACKDPEEEEL 
--------------------------------------------------------------MRIA---VICFCLLGITCAIPV-------KQAD--SGSSEE--K-QL--------YNKYPDAVAT--------------------WLNPDPSQKQNLLAPQNAV-SSEETN-D-FKQETLPSKSHDHMDDMDDE-DDDDH--VDSQDSIDS-----------------NDSDDVDDT--DDSHQSDESHH------------------SDE----------SDELVTDFPTDLPA--TEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDI---TSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDD-QSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN------------- 
 
NDPSQVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKEDLEKAKKEHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSETKEPYRLEGAPEAQLLLRVEIVNGLADYARNYAQAIRDAINLGAKVINSFGNAALAYANLPDETKKAFDYAKSKGVSIVTSAGNDSSFGGKTRLPLADHPDYGVVGTPAAADSTLTVASYSPDKQLTETVRVKTADQQDKEPVLSTNRFEPNKAYDYAYANRGT------------------------------------------------------------------------------KEDDFKDVKGKIALIERGDIDFKDK-------IAKAKKAGAVGVLIYDNQDKGFPIELPNVDQPAAFISRKDGLLLKDNPQKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAKLSGTSSAPLVAGIGLLQKQYETQYPDTPSERLDLAKKVLSSATALYDEDEKAYFSPRQQGAGAVDAKKASAATYVTDKDNTSSKVHLNNVSDKFEVTVNVHNKSDKPQELYYQATVQTDKVDGKHFALAPKVLYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQKNGYFLEGFVRFKQDPTKEELSIPYIGFRGDFGNLSAL-------------EKPIYDSKDGSSYYHEANSDAKDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGTFAKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGNVVWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPISS----------------GAKEQ---HTDFDVIVDNTTPEVATSATFSTEDRRLTLASKPKTSQPVYRE-RIAYTYDEDLPTTEYISPNEDGTFTLPEEAE-------------------TEGA----------------------------------------------TVPLKSDFTYVVEDAGNITYTPVTKLLEGH------------- 
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSHDHMD--------DMDDE-------DDDDHVDSQDSIDSNDSDDV---------------------DDT-------------------------D-DSH----------------------------------------------------------------------------------QSD--ESHHSD---E--SD--------------------------------------------------ELVTD-----------------------------------------------------FP--------------------TD-LP-------ATEVFTPVVPTVDTYDGR-----------------GDSVVYGLRSK-----------------------------------SK-KFR-R------------------------P-----------DIQ----------------------------------------------------------------------YPDAT-----DEDITSHMESEELNGAYKAIPVAQDLNAPSDW------------------------------D-SR-GKDSYETSQ-----------------------LDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFR---------------------------ISHELDSASSEVN 
 
KTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAGKPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTKNNVFVGYDKDGWFWEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVNDHLKNEKKILLKAGSYDDERTVVSVKTDNQEETGPEVDDSKVTYDTIQSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAEYLKLRDDAHL---------------------------------------------------------------------------------------INAETVRLQVVDNQLHFDVTKIVNHNQVTPG-------QKIDDERKLLSSISFLGNALVSVSSDQTGAKFDGATSNNTHVSGDDHIDVTNPKDLAKGYYGFVSTDKLAAGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKELPSAKVVITEDANADKKVDWQDGAIAYRSINNPQGWKKVKDITAYRIANFGSQAQNPFLTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIEKAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLAHGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWGHGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAAN--------------YPLLGGYSKD--FEGWQGRSDYNGYVTNLFAHDVTKYFQHFTVSKWENGTPVTTDNGSTYKWT--PERVELVDADNNKVVV------------TR-KSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDANGKKLSTDKEKYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKITLDLLANQPYVLYRSKQTNPESWSEGHIYDQGFNSGTLKHWTISGDASKAEIVKSQGANDLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYTNKSLALNYVKAYAHNTRRNNATVDDTSYFQN----------------YAFFTTGSDVSNVTLTLSREAGDEATYFDEIRTFENNSSYGDKHDTGK-----------GTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEKHDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLV-YQTIPQNFRFEAGKTYRVTFEYEAGSDNTYAFVVGKGEFQSGRRGTQASNLEHELPNTWTDSKKAKKATFLVTGAETGDTWVGIYSTGNASNTRGDSGGNANFRGYNDFDNLQIEEITLTGKLTENALKNYLPTVAKEAVFNLSQADDDISVEEARAEIAKI- 
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSHDHMDDMDDE-D------------------DDDH-VDSQDSIDSNDSDDV--D-DTD-DSH-QSD-E----------------------------------------------------SH---------------HSDES-D-------ELV---------------------------T-D---------FPT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRR--PD--IQY------------------------------PDATDE------------DITSHMESEELNG-AYKAIPVA-QDLN------------AP------------------------SD-----------------------------------------------WDS------------------------R----------------------GKDSY----------------------------ETS-------QL---------DDQ--------------------------------SAETHSHKQSRLYKRKAND--------------------------ESNEHSDVID-SQE-----LSKVSREFHSHEFHSHE--DMLVVD---------------PKS-------------------------KEE-----------DKHLKFRISH--------E---------------------------------------------------------------------------------------------LDSASSEV-------------------------------------------N 
 
LNLDPVQLTFYAGPNGSQFGFSLDFHKDSHGRVAIVVGAPRTLGPSQEETGGVFLCPWRAEGGQCPSLLFDLRDETRNVGSQTLQTFKARQGLGASVVSWSDVIVACAPWQHWNVLEKTEEAEKTPVGSCFLAQPESGRRAEYSPCRGNTLSRIY---------------------------------------------------------------------VENDFSWDKRYCEAGFSSVVTQAGE-------LVLGAPGGYYFLGLLAQAPVADIFSSYRPGILLWHVSSQSLSFDSSNPEYFDGYWGYSVAVGEFDGDLNTTEYVVGAPTWSWTLGAVEILDSYYQRLHRLRGEQMASYFGHSVAVTDVNGDGRHDLLVGAPLYMESRADRKLAEVGRVYLFLQPRGPHALGAPSLLLTGTQLYGRFGSAIAPLGDLDRDGYNDIAVAAPYGGPSGRGQVLVFLGQSEGLRSRPSQVLDSPFPTGSAFGFSLRGA-VDIDDNGYPDLIVGAYGANQVAVYRAQPVVKASVQLLVQDSLNPAVKSCVLPQTKTPVSCFNIQMCVGATGHNIPQKLSLNAELQLDRQKPRQGRRVLLLGSQQAGTTLNLDLGGKHSPICHTTMAFLRDEADFRDKLSPIVLSLNVSLPPTEAGMAPAVVLHGDTHVQEQTRIVLDCGED-DVCVPQLQLTASVTGSPLLVGADNVLELQMDAANEGEGAYEAELAVHLPQGAHYMRALSNVEFERLICNQKKENETRVVLCELGNPMKKNAQIGIAMLVSVGNLEEAG-ES-V-SFQLQIRSKN--------------S--Q---NPNSKIVL-LDVPV---RAEAQVELRGNSFPASLVVAA---SW--GP-KVEHTYELHNNGPGTVNGLHLSIHLPGQSQPSDLL--------------------YILDIQPQGGLQCFPQPPVNPLKVDWDPVLVSCDSAPCTVVQC-----DLQEMARGQR------AMVTVLAFLWLPSLYQRPLDQFVLQSHAWFNVSSLPYAVPPLSLPRGEAQVWTQLLRAC 
-----------------------------------------------------------------------------------------------------------------------------------------------------------MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETL--P-SKSHDHMDDMDDE---DDDDHVDSQDSI--------DSNDSDDV-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DDTDDSHQS------------------DESH---------------------------------------HSDESDELV----------------------------------T--D-FPTDLPATEV--------------------------------------------F---------TPV-VP---------------------------------T--VDTYDGRG--DS-----------------------V----------------------VYGLRSKS-KKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPS-DW----DSRGKDSYETSQLDDQS--A----ETHSHKQS-RLYKRKANDESN------------------------EHSDVIDSQELSKVSREFHSHEFHSHEDMLV--------------------------------VDPKSKEEDKH----LKFRISHELDSASSEV--N---------------------------------------------- 
 
IDALTKDQRALFKQQLEIIARYLKMDLRAGDKAFVASQESQKALQAQLDLWQAEHGDIYAAGIEPAFDPLKARVYDSSWNWARQDALSMYYDIIFGRLRVVDREIVSQCIQIMNRSNPLLLEFMQYHIDHCPTERGETYQLAKELGQQLIENCKEVLGKPPVYKDVSIPTGPQTTIDARGNIQYQEVPRASARKFEHYVKQMAEGGPISQYKVETIPFLHLRKKDEFGNWEYSKKLTGIYLDGLEAAARSGLTFQGKHALMTGAGAGSIGAEVLQGLLSGGAKVIVTTSRFSRQVTEYYQGIYARCGARGSQLVVVPFNQGSKQDVEALVNYIYDTKNGLGWDLDYVVPFAAIPENGREIDSIDSKSELAHRIMLTNLLRLLGAIKTQKKERGYETRPAQVILPLSPNHGTFGNDGLYSESKLALETLFNRWYSESWGNYLTICGAVIGWTRGTGLMSANNLVAEGVEKLGVRTFSQQEMAFNLLGLMAPAIVNLCQSDPVFADLNGGLQFIPDLKGLMTKLRKEIMETSAIRQAVIKETAIENKVVNGEDHEALYRRVITEPRANLKYPFPELPDWDKDIKPLNDQLRGMVNLDKVVVVTGLAEIGPWGNARTRWEMEAYGKFSLEGCVEMAWMMGLIKNHNGPLKGKPYSGWVDAKTGEPVDDKDVKAKYEKYILEHSGIRLIEPELFGGYDPNRKQLLQEVVIEQDLEPFEASKEQAEEFKREHGDKVEIFEIPETGQYTVRLRKGATLLIPKA-----------------------------------------------------------------------------------------------------------------------------------------------LQFDRLVAGQIPTGWDARRYGVPEDIIQQVDPVTLYVLVSVAEALLSSGITDPYEFYKYVHLSEVGNCIGSGVGGTSALRGMYKDRYLDKPVQKDILQESFVNTMAAWVNMLLLSSTGPIKTPVGACATAVESLDVGYDTIMQGKARVCLVGGFDDFQEEGSYEFANMGATSNAKEEFARGREPGEMSRPTSTTR--------NGF-----MESQGCGVQVIMTAQLALEMGVPIYGIVAMTSTATDKIGRSVPAPGQGVLTTAREKSGNFPSPLLDIKYRRRQLELR-----------------------------RQQIKQWKESEYLYLQEEVAAIKSQRSEEDGPF--------DETAYLRERTEHIEREARRQEAEAQTSFGNEFWRRDSRIAPLRGALATWGLTIDDLGVASFHGTSTVANDKNESDVICQQLKHLGRTKGNAVLGIFQKYLTGHPKGAAGAWMLNGCLQVLNTGIVPGNRNADNVDKVMEQFDYIVYPSRSIKTDGIKAFSVTSFGFGQKGAQAIGVHPKYLFATLDKAQYEAYCVKVQARQKKAYRFFHNGLINNKLFVAKDKAPYEDRIQSKVFL-------NPQSRVTQESNGELKFPA-------------- 
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVP-TVDT--------------------------------------------------------------------------------------------------------------------YDGRG-DSVVYGLRS---KSKKFRRPDIQYP----D--A-----TDEDI--T----SHM----------ESEELNGAYKAIPVAQDLNAPSDWDSRGK--DSYE----------------------------------------------------------TSQLDDQSAETHSHKQSRLYKRKANDESNE--HS--D--VI--DSQ-ELSK-----------VSREFHSHEF--HS--HE--DMLV--VD------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PKSKEEDKHLKF-------------RISHELDSASSEVN 
 
QSVVSRTPIPLSKIGLQDVKKLFDINVIKCGSSLRIVDEPQVTFIVSYAKDIYDKFMCIEHDSAYEPSLTMHRVRVIYSMLNDYCAKMISE-VPYESSFVGELPVKSVTLNKLGDRNMDALAEHLLF----EHDVVNA-QRENRIFYQRKSAPAVPVIFGDDLEPAVRERANLYHRYSVPYHQIELALHALANDLLSIQYCHPTVVYNYLSSRAPNFLRLDDQVSLKLTSAGIGTLMPRPVVQLLDYDLVYMSPLALNNLASRLLRKISLHLVMQMVTAVQQDLGEVVSVSSNVTNPASACLVRMNVQGVQTLAVFIAQSMLNPNISYGMISGLTLDCFSNFIYGACLMLFQALIPPSALTARQRLDINNRFAY----------------------------------------------------------------------------------------------------------------FLIKCHATQAT-----------------------------------------TARLVANQVIYPVDAIDQWQSNGRDVLVAIYNNLLPGELVLTNLIQTYFRGNTAQQAAEILIPADQTSYGA---------------NETRALSAPYLFGAPINMLAPDARLSTYKRDLALPDRSPILITTVEGQNSISIENLRHKTGLIRAMYLNGFVTQPPAWIRNANSNTALLSRFLDATPNLLGIYEAILANTYANAVNVYC------------DSVYRADIPIEWKLHQSVDPQDLLFGVFGIVPQYQILNEAVPDFFAGGEDILILQLIRAVYDTLSNKLGRNPADIFHLEEVFKVIEEIVSVLVQQKIDVRKYFTESMRSGSFSKPRWDNFLRRPVAQRLPNLYSVIMTQADHVYNYMTQLTHIIPITDCFYIVKNSGFVDRGSTGPVIASSSVYENVLKVVHTIADFDAANALRLQRRRVDNTSYTDSLSDMFNGLRSISSSEFVRSVNGRSVFTEGRIDAIKVNMRAKFDLQFITEEGGYSKPPNVKKLMFSDFLSFLDSHKSDYRPPLLTVPITIGLNNLGETNSNTLRMRSEAIDEYFSSYVGAQILVPINVVDTRVYTEFSELRNFFTGDVVIRDDPFDVWDGVKATYIPIGVHGVRLDPNGDQ 
-------------------------------------------------------------------------------------------MRIAVICFCLLGI-----------------------TCAIPVKQADSGSSEE-KQ-L-YNKYPD---------AVAT-----------------------------------W------------------------LNPDPSQ-------------------------------------------------------------------------------------------------------------------------------------------KQNLLAPQNAVSSEETNDFKQETLPSKSHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGK---DS---------------Y-E-----------------------------TSQLDDQ-------SAETHSHKQSRLYKRKANDES-NE--HSD---VIDSQELSKVSREFH-SH-EFHSHED----------ML--VV--DP-----------------------------K--S--K--------------------EEDKHLKFRISHELDSAS-S------------------------------------------E--------------------------------------------------------------------V--N----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 
 
GLVPRGSHMMEILRGSPALSAFRINKLLARFQAANLQVHNIYAEYVHFADLNAPLNDSEQAQLTRLLQYGPALSSHTPAGKLLLVTPRPGTISPWSSKATDIAHNCGLQQVDRLERGVAYYIEASTLTAEQWRQVAAELHDRMMETVFSSLTDAEKLFIHHQPAPVSSVDLLGEGRQALIDANLRLGLALAEDEIDYLQEAFTKLGRNPNDIEL----------------------------------------------------------------------------YMFAQANSEHCRHKIFNADWIIDGKPQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSAVGRYFADHNTGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVGFSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALTGYFRTYEEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEIVVGAKLIVLGGPAMNIGFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVG------AGGLSNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDELCKRERAPYAVIGDATEEQHLSLHDNHFDNQPIDLPLDVLLGKTPKMTRDVQT---LKAKGDALNRAD----------------------------ITIADAVKRVLHLPTVAEKTFLVTIGDRTVTGMVARDQMVGPWQVPVADCAVTTASLDSYYGEAMSIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANWMAAAGHPGEDAGLYDAVKAVGEELCPQLGLTIPVGKDSMSMKTRWQEGNEQREMTSPLSLVISAFARVEDVRHTLTPQLSTEDNALLLIDLGKGHNALGATALAQVYRQLGDKPADVRDVAQLKGFYDAMQALVAARKLLAWHDRSDGGLLVTLAEMAFAGHCGVQVDIAALGDDHLAALFNEELGGVIQVRAEDRDAVEALLAQYGLADCVHYLGQALAGDRFVITA--------------------------NDQTVFSESRTTLRVWWAETTWQMQRLRDNPQCADQEHEAKANDTDPGLNVKLSFDINEDIAAPYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLGGRIGLGNFH----ALVACGGFSYGDVLGAGEGWAKSILFNHRVRDEFETFFHRP-QTLALGVNGCQMMSNLRELIPGSELWPRFVRNHSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVRDDAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTENG---------------RVTIMMPHPERV--FRTVANSWHPENWGEDSPWMRIFRNARKQLG 
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSH-DH-MDD---MD--DEDD--D---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DHVDSQDS---ID--SN--D---------------------------------------------------------------------------------------S-D-DVDDTDDSHQSDESHHS-------DESDELVTDFPTDLPATEVFTPVVPTVDTYDG-RG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DSVVYGLRSKSKKFRRPDIQY--PDA-----------------------------------------------------TDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRG--------------------------------K-DSYETSQLD----------------------------------------------------------------DQSAE-----------------------------------THSHKQSRL------------------------------------------------YKRK-A---NDESNEHSDVI-----------------------------------------------------DSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDK-HLK--F---RISHELDSAS-SEVN 
 
QKGPVFLK-------------------------------------------------------------------------------EPTNRIDFSNSTGAEIECKASGNPMPEIIWIRSDGTAVGDVPGLRQISSDGKLVFPPFRAEDYRQEVHAQVYACLARNQFGSIISRDVHVRAVVAQYYEADVNKEHVIRGNSAVIKCLIPSFVADFVEVVSWHTDEEENYFPGAEYDGKYLVLPSGELHIREVGPEDGYKSYQCRTKHRLTGETRLSATKGRLVITEPISSAVPKVVSLAKFDMKTYSGSSTMALLCPAQGYPVPVFRWYKFIEGTTRKQAVVLNDRVKQVSGTLIIKDAVVEDSGKYLCVVNNSVGGESVETVLTVTAPLSAKIDPPTQTVDFGRPAVFTCQYTGNPIKTVSWMKDGKAIGHSESVLRIESVKKEDKGMYQCFVRNDRESAEASAELKLGGRFDPPVIRQAFQEETMEPGPSVFLKCVAGGNPTPEISWELDGKKIANNDRYQVGQYVTVNGDVVSYLNITSVHANDGGLYKCIAKSKVGVAEHSAKLNVYGLPYIRQMEKKAIVAGETLIVTCPVAGYPIDSIVWERDNRALPIN--------------------------RKQKVFPNGTLIIENVERNSDQATYTCVAKNQEGYSARGSLEV---QVMVLPRIIP---FAFE-EGPAQVGQYLTLHCSVPGGDLPLNIDWTLDGQAISEDLGITTSRV-------------------------GRRGSVLTIEAVEASHAGNFTCHARNLAGHQQFTTPLNVYVPPRWILEPTDKAFA----QGSDAKVECKADGFPKPQVTWKKAVGDTPGEYKDLKK---------------SDNIRVEEGTLHVDNIQKTNEGYYL-------CEAINGIGSGL-SAV----IMISVQA 
--------MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSHDHMDD-------------------------MDDEDDDDHV-------------------------------D---------------SQDSI------------------------------DSNDS----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DDVDDTDDSHQSDESHHSDESDELVTDFPTD-------L-------P---AT-------------EVFTPVVPTVDTYDGRGDSVVYGLRSKSK----KFRRPDIQYPDATD-----------------------EDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDS---------------------------YETSQ--LDDQSAETHSHKQSRLYKRKANDESNEHSDV------------------IDSQELSKVSREFHSHEFHSHEDML---------------VVDPKSKEEDKH----LKFRISHELDSASSEVN 
 
SDVLELTDDNFESRISDTGSAGLMLVEFFAPWCGHAKRLAPEYEAAATRLKGIVPLAKVDCTAN----------------------------------------------------------------------------TNTCNKYGVSGYPTLKIFR------DGEEAGAYDGPRTADGIVSHLKKQA-------------GPASVPLRTEEEFKKFISDKDASIVGFFDDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIILFRPSHLTNKFEDKTVAYTEQKMTSGKIKKFIQENIFGICPHMTEDNKDLIQGKDLLIAYYDVDYEKNAKGSN----------------YWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGEKF-VMQEEFS-R-------------------------------DGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNI--------------VIAKMDATANDVPSPYEVRGF----------------------PTIYFSPANKK----LNPKKYEGGRE--------LSDFISYLQREATNPPVIQEE 
----------------------------------------------------------------MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKS--HD-HM----D-DMDDEDDDDHVDSQD--------------------SIDSNDSDDVDDTDDSHQSD----ES--HH-------S-D--------------------------ESDELVTD-FP---------------------------------------------------------------------------------TDLPATEVFTPVVPTVDT-YDG--R-------------------------------------GDSVVYGL-RSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDW-DS---------R-GK-D-SYE--------TS-----------------Q----------------LDDQS---------AETHSHKQSRLYKRKANDESNE---------HSDVIDSQELSKVSREFHSHEFHSHEDMLV-------VDPKSKEEDK-----HLKFRISHELDSAS--SE---------VN 
 
MADHSASGAPALSTNIESGKFDEKAAEAAAY-----------------------------------------------------------------------QPKPKVEDDEDEDIDALIEDLESHDGHDAEEEEEEATPGGGRVVPEDMLQTDTRVGLTSEEVVQRRRKYGLNQMKEEKENHFLKFLGFFVGPIQFVMEGAAVLAAGLEDWVDFGVICGLLLLNAVVGFVQEFQAGSIVDELKKTLALKAVVLRDGTLKEIEAPEVVPGDILQVEEGTIIPADGRIVTDDAFLQVDQSALTGESLAVDKHKGDQVFASSAVKRGEAFVVITATGDNTFVGRAAALVNAASGGSGHFTEVLNGIGTILLILVIFTLLIVWVSSFYRSNPIVQILEFTLAITIIGVPVGLPAVVTTTMAVGAAYLAKKKAIVQKLSAIESLAGVEILCSDKTGTLTKNKLSLHDPYTVAGVDPEDLMLTACLAASRKKKGIDAIDKAFLKSLKYYPRAKS-----------VLSKYKVLQFHPFDPVSKKVVAVVESPQGERITCVKGAPLFVLKTVEEDH-PIPEEVDQAYKNKVAEFATRGFRSLGVARKRGEGSWEILGIMPCMDPPRHDTYKTVCEAKTLGLSIKMLTGDAVGIARETSRQLGLGTNIYNAERLGLGGGGDMPGSEVYDFVEAADGFAEVFPQHKYNVVEILQQRGYLVAMTGDGVNDAPSLKKADTGIAVEGSSDAARSAADIVFLAPGLGAIIDAL--------------------------------------------------------------------------KTSRQIFHRMYAYVVYRIALSIHLEIFLGLWIAILNRSLN---IELVVFIAIFADVATLAIAYDNAPYSQTPVKWNLPKLWG-MS--V-LLGVVLAVGTWITVTTMYAQGENGGIVQNFGNMDEVLFLQISLTENWLIFITRANGPFWSSIPSWQLSGAIFLVDILATCFTIWGWFEHSDTSIVAVVRIWIFSFGIFCIMGGVYYILQDSVGFDNLMHGKSPKGNQKQRSLEDFVVSLQRVSTQHEKSQ 
-------------------------------MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPS------------------------------------------------------KSH-------------------------------------------------------------------------------------------------------DHMD-DMDDE------------DDDD------------------HV--------------DSQD-SIDSNDSDD------------------------------------------------------------------------------------------------------------------------------------------------------------------------VDDTDDSHQSDESHHSDES----------------DELVTDFPT------------DLPATEVFTPVVPT--VDT-Y--DGRG------------------------D-S---VV--YG-LR---SKS------------------KKFRRPDIQ-------------------------------------------------------------------------------------------------YPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNE--HS--DV-ID--SQ--EL-------------------SKVSREFHSHE--FH--SH-EDMLV--VD---PK-S---KEEDK-HLKFRISHELDSA--SS--EV---N------------------------------------------------------------------------------------------------------------------------------------------- 
 
(a)Residue pairs that are structurally aligned by TM-align program are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade.(more about the colors used)
(b)Ranking of proteins is based on TM-score of the structural alignment between Model1 and the PDB structures in our template library.
(c)RMSDa is the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.

  Function Prediction

  Predicted EC Numbers

RankTM-scoreRMSDaIDENaCov.EC-ScorePDB
Hit		EC No.
10.3421 5.32 0.05 0.500.24031xf1A 3.4.21.110
 
20.2869 5.66 0.06 0.440.22512nztA 2.7.1.1
 
30.2773 6.44 0.06 0.470.21441mhsA 3.6.3.6
 
40.2706 6.40 0.05 0.460.20493ffzB 3.4.24.69
 
50.2833 6.03 0.05 0.450.20461qhaA 2.7.1.1
 
(a)Ranking is based on EC-score, which is a confidence score for the Enzyme Classification (EC) Number prediction.
(b)RMSDa is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)IDENa is percentage sequence identity in the structurally aligned region.
(d)Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)EC-Score is defined based on the C-score of the structure prediction and similarity of the model with known enzyme structures, as identified using both global and local structural alignment programs. The global similarity score uses TM-score, IDENa,RMSDa and Cov. of the structural alignment by TM-align, while the local match compares the structural and chemical similarity of local spatial motifs in the model with known catalytic site of enzymes. A prediction with a EC-score >1.1 signifies a prediction with high confidence (upto 3 digit numbers of EC) and vice versa (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).

  Predicted GO terms

RankTMscoreRMSDaIDENaCov.PDB
Hit		Fh-ScoreAssociated GO Terms
10.1855 5.12 0.17 0.272pilA 0.23 GO:0005515 GO:0008565 GO:0007155 GO:0015628 GO:0022610 GO:0032940 GO:0051641 GO:0051649 GO:0005623 GO:0009289 GO:0015627
20.3415 5.29 0.04 0.501xf1B 0.22 GO:0004175 GO:0004252 GO:0006508 GO:0009405 GO:0009987 GO:0034960 GO:0043283 GO:0044237 GO:0005576 GO:0005618 GO:0005623 GO:0009986 GO:0016020
30.2943 6.43 0.05 0.501ej6C 0.22 GO:0004386 GO:0005198 GO:0005524 GO:0008270 GO:0030554 GO:0043169 GO:0006370 GO:0006397 GO:0009987 GO:0010467 GO:0016071 GO:0034960 GO:0043283 GO:0044237 GO:0005622 GO:0005623 GO:0019012 GO:0019013 GO:0019028
40.2431 6.64 0.08 0.422bk2A 0.22 GO:0015485 GO:0001906 GO:0009405 GO:0009987 GO:0019835 GO:0019836 GO:0044004 GO:0051701 GO:0051702 GO:0051715 GO:0051818 GO:0051851 GO:0052025 GO:0052111 GO:0052331 GO:0052332
50.2648 5.53 0.07 0.402bg9E 0.22 GO:0004889 GO:0005231 GO:0008324 GO:0022834 GO:0022838 GO:0022892 GO:0030594 GO:0042165 GO:0006811 GO:0005623 GO:0005886 GO:0016021 GO:0030054 GO:0045202 GO:0045211
60.2951 6.99 0.04 0.531uf2A 0.21 GO:0005198 GO:0019012 GO:0019013 GO:0019028
70.3121 5.88 0.04 0.491yq2F 0.21 GO:0004565 GO:0030246 GO:0043169 GO:0005975 GO:0009341
80.2799 6.24 0.05 0.451u8cA 0.21 GO:0004872 GO:0005509 GO:0005515 GO:0043169 GO:0001568 GO:0007154 GO:0007160 GO:0007229 GO:0010324 GO:0022610 GO:0043277 GO:0044419 GO:0048856 GO:0065007 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005887 GO:0005912 GO:0005925 GO:0008305 GO:0009897 GO:0009986 GO:0016021 GO:0016323
90.2757 6.31 0.05 0.471mhsB 0.20 GO:0000287 GO:0005524 GO:0008553 GO:0015405 GO:0015662 GO:0022892 GO:0030554 GO:0042626 GO:0043492 GO:0006754 GO:0006818 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009987 GO:0015992 GO:0044237 GO:0046034 GO:0005623 GO:0005886 GO:0016021
100.2686 6.33 0.05 0.441zvoD 0.20 GO:0003823 GO:0002376 GO:0006955 GO:0005576 GO:0005623 GO:0005886 GO:0016021

Consensus Prediction of Gene Ontology terms 
Molecular Function  Biological Process Cellular Location
GO termGO-Score  GO termGO-Score GO termGO-Score
GO:00054880.171  GO:00099870.150  GO:00056230.170
GO:00038240.086  GO:00081520.106  GO:00444640.170
GO:00167870.086  GO:00442380.106  GO:00160200.105
GO:00431670.084  GO:00512340.105  GO:00312240.083
GO:00228920.065  GO:00068100.105  GO:00444250.083
GO:00052150.065  GO:00511790.105  GO:00160210.083
GO:00431690.064  GO:00442370.085  GO:00058860.083
GO:00468720.063  GO:00444190.065  GO:00329910.064
GO:00051980.044  GO:00517040.065  GO:00432340.064
GO:00055150.043  GO:00431700.064  GO:00056220.063

(a)Ranking in the first table is based on a function prediction score (Fh-score), which is calculated based on the C-score of the structure prediction and the TM-score, IDENa, RMSDa and Cov. of the structural alignment by TM-align between the predicted model and the PDB structures (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).
(b)RMSDa is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)IDENa is the percentage sequence identity in the structurally aligned region.
(d)Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)A consensus prediction of GO terms is derived from the structural analogs that have an Fh-score of >=1.0. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on the Fh-score of the template from which the GO term is derived. A prediction with a GO-score >0.5 signifies a prediction with high confidence and vice versa.


  Predicted Binding Site


Binding site residues in the model:
SER:277  GLU:290  HIS:301  GLU:302  ASP:304  
Identified analogs with similar binding site:

RankPDB
Hit		TM-scoreRMSDaIDENaCov.BS-scoreDownload
Complex		Binding site residues on the predicted model
12rj7A0.1827 6.36 0.06 0.30 0.43Download277,290,301,302,304
22aazA0.1914 6.09 0.05 0.31 0.37Download249,284,286,292,293,294
32v6oA0.2140 6.23 0.05 0.35 0.36Download155,158,159,160,163,164,168
42uvnA0.2129 5.90 0.01 0.33 0.33Download223,224,225,249,250,251,252,294,295
51f4uA0.2025 6.26 0.02 0.32 0.27Download223,224,225,226,246,247,248,249,250,252,282,283,284,292,293,294,295,296,297,298,299
62uvnA0.2129 5.90 0.01 0.33 0.25Download224,225,226,247,248,249,250,252,253,254,282,283,284,292,293,294,295,296,297,299
72dq3A0.1916 6.89 0.04 0.34 0.25Download228,229,230,244,245,246,247,248,249,278,293,295,299,301,302,303,304,305
82rj7A0.1827 6.36 0.06 0.30 0.19Download229,230,231,232,233,242,243,244,245,246,274,275,276,277,278,302,303,304,305,306,307,308
91egyA0.1838 6.28 0.05 0.30 0.18Download223,224,225,226,247,248,249,250,252,253,254,282,283,284,292,293,294,295,296,297,299
101j1wA0.2045 6.60 0.04 0.35 0.17Download252,253,254,255,257,259,280,281,282,283,284,292

(a)Ranking of the analogs in the table is based on their BS-score. BS-score is calculated by first finding the structural analogs of the predicted model based on their TM-score, IDENa and Cov. of the structural alignment. The ligand(s) in the analog structure are then transferred onto the model and the fitness of the ligand-model complex (BS-score) is calculated by comparing the local structure and sequence similarity in the binding site region. (For detail, see Kucukural, Szilagyi, Ambrish, and Zhang, Template based ligand binding site prediction on modeled protein structure, 2009, in preparation).
(b) A BS-score of >0.5 signifies a binding site prediction with high confidence and vice-versa.
(c)RMSDa the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.
(f)The image shows the ligand-protein complex with the best BS-score. The ligand is depicted in magenta colored ball & stick, the predicted binding site residues interacting with the ligand are shown as transparent green spheres, while the N & C terminus in the model are marked by blue and red spheres respectively.



 Please cite following articles when you use the I-TASSER server:
1.Yang Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9:40 (2008).
2.Yang Zhang. Template-based modeling and free modeling by I-TASSER in CASP7. Proteins, 8: 108-117 (2007).
3.Sitao Wu, Jeffrey Skolnick, Yang Zhang. Ab initio modeling of small proteins by iterative TASSER simulations. BMC Biology, 5:17 (2007).