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I-TASSER results for job id S34197

  Submitted Sequence

>HsSAS6
MSQVLFHQLVPLQVKCKDCEERRVSIRMSIELQSVSNPVHRKDLVIRLTDDTDPFFLYNL
VISEEDFQSLKFQQGLLVDFLAFPQKFIDLLQQCTQEHAKEIPRFLLQLVSPAAILDNSP
AFLNVVETNPFKHLTHLSLKLLP

  Predicted Secondary Structure

Sequence                  20                  40                  60                  80                 100                 120                 140
                   |                   |                   |                   |                   |                   |                   |    
MSQVLFHQLVPLQVKCKDCEERRVSIRMSIELQSVSNPVHRKDLVIRLTDDTDPFFLYNLVISEEDFQSLKFQQGLLVDFLAFPQKFIDLLQQCTQEHAKEIPRFLLQLVSPAAILDNSPAFLNVVETNPFKHLTHLSLKLLP
PredictionCCCSSSSSSSSSSSSCCCCCCCCSSSSSSSSSSSCCCCCCCCSSSSSSCCCCCCCSSSSSSSCHHHHHHHHHHHCCSSCHHHHHHHHHHHHHHHHHCCCCCCCSSSSSSSSCCCCCCCCCSSSSSSSSCCCCSSSSSSSSSCC
Conf.Score94078887899999777887544687699999827888776059999987886524589997689999998871714608897999999999986553677856999997301446798399999986688779999999409

  Top 5 Models predicted by I-TASSER

Download Model 1 Download Model 2 Download Model 3 Download Model 4 Download Model 5
C-score=-3.28
 C-score=-3.56
 C-score=-4.78
 C-score=-4.81
 C-score=-4.93
 
Estimated accuracy of Model1: 0.35±0.12 (TM-score)    12.2±4.4Å (RMSD)    (Read more about C-score of generated models)

  Top 10 templates used by I-TASSER

RankPDB
Hit		Iden1Iden2Cov.Norm.
Z-score		Download
Align.		                   20                  40                  60                  80                 100                 120                 140
                   |                   |                   |                   |                   |                   |                   |   
Sec.Str
Seq		CCCSSSSSSSSSSSSCCCCCCCCSSSSSSSSSSSCCCCCCCCSSSSSSCCCCCCCSSSSSSSCHHHHHHHHHHHCCSSCHHHHHHHHHHHHHHHHHCCCCCCCSSSSSSSSCCCCCCCCCSSSSSSSSCCCCSSSSSSSSSCC
MSQVLFHQLVPLQVKCKDCEERRVSIRMSIELQSVSNPVHRKDLVIRLTDDTDPFFLYNLVISEEDFQSLKFQQGLLVDFLAFPQKFIDLLQQCTQEHAKEIPRFLLQLVSPAAILDNSPAFLNVVETNPFKHLTHLSLKLLP
11ab4A 0.13 0.22 0.92 1.02Download IAELVKEKRVEGISALRD-ESDKDGMRIVIE------GEVVLNNLYSQTQLQVSFGINMVALHHGQPKIMNLKD-IIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP---IIELIRHAP-TALVANPWQLGN
21qynA 0.08 0.16 0.73 0.49Download --QRIYTKDISFEAPNQKDWQPEVKLDLDTASSQLADDVYEVVLRVTVTASLGEETAFLCEVEGTQMAHCLG---AYCPNILFPYCITSMVSRG-----TFPQLNL-APDALFYLQ---------------------------
31ik9A 0.15 0.15 0.74 0.62Download --------MERKISRIHLVSEPSITHFLQVSW----EKTLESGFVITLTDGHS---AWTGTVSESEISQEADDMAME------KGKYVGELRKALLSASKESAYFFFEKNLKDVSFRLGSFNLEKVE----------------
41ik9A 0.14 0.15 0.79 0.82Download MERKISRIHL------------VSEPSITHFLQVSWEKTLESGFVITLTDGHS---AWTGTVSESEISQEADDMAME------KGKYVGELRKALLSADVYTFNFS---------KESAYFFFEKNLKDVSFRLGSFNLEKVE
51ik9A 0.16 0.15 0.74 0.97Download ------MERKISRIHLVS------EPSITHFLQVSWEKTLESGFVITLTDGHS---AWTGTVSESEISQEADDMAME------KGKYVGELRKALLSADKESAYFFFEKNLKDVSFRLGSFNLEKVE----------------
61zo2A 0.11 0.13 0.50 0.96Download ------------------------------------------------------------------------SINLNPQFDQIGKQFVQHYYQTFQTNRPALGGLYGPQSMLTWEDTQFQGQVNKFNSLNFQQFEITRVDCQP
72i15A 0.17 0.17 0.84 0.78Download PQLLALKQFVQTEFEKVDFETFRQNFNRCLEREQSTLLIYEDD------DYDDQSFFLKPMLSDAFFISSEVVKQL-----DLPK---GDVKSCCQSFYEALTLFISALAITKGV-DVGRYHQQLGKRFGVLTVY--------
81yqfA 0.08 0.16 0.58 0.39Download ------GEDLVVRYSTNQDSDKANSHNIFVYI----TQKNGQTMQADLSIEEGELVLNNIRFDYDCVMTYLEKRGVDEKLGEFVVLYSFWAEQ--------------------------------------------------
91nqeA 0.08 0.14 1.00 0.38Download DAWSGFVRGYGYDNRTNDTRKLYSQSWDAGLRYNGELIKSQLITSYSHSKDYNYATLDEMKQYTVQWANNVIVGHGSIGAGVDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGTWQTSAGWEF
101zvuA 0.13 0.16 0.96 1.00Download IAAQMRNKKLPMVDDLRDESDHENPTRLVIVPRSNRDMDQVMNHLFATTDLEKSYRINLNMILDGRPAVKNLLE-ILSEWLVFRRDTVRRRLNYRLEKSERKMNNLLKKE-----LQADAQAYGDDRRSPLQEREEAKADMLP
(a)All the residues are colored in black; however, those residues in template which are identical to the residue in the query sequence are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade. (more about the colors used)
(b)Rank of templates represents the top ten threading templates used by I-TASSER.
(c)Ident1 is the percentage sequence identity of the templates in the threading aligned region with the query sequence.
(d)Ident2 is the percentage sequence identity of the whole template chains with query sequence.
(e)Cov. represents the coverage of the threading alignment and is equal to the number of aligned residues divided by the length of query protein.
(f)Norm. Z-score is the normalized Z-score of the threading alignments. Alignment with a Normalized Z-score >1 mean a good alignment and vice versa.
(g)Download Align. provides the 3D structure of the aligned regions of the threading templates.
(h)The top 10 alignments reported above (in order of their ranking) are from the following threading programs:
       1: MUSTER   2: HHSEARCH   3: SP3   4: PROSPECT2   5: PPA-I   6: HHSEARCH I   7: FUGUE   8: HHSEARCH II   9: SPARKS   10: MUSTER   

  10 proteins in PDB which are structurally closest to the first I-TASSER model (identified by TM-align)

RankTM-scoreRMSDaIDENaCov.PDB
Hit
10.5581 4.05 0.08 0.842b39A
Model1
 
20.5574 4.07 0.07 0.842a73B
Model1
 
30.5504 4.15 0.10 0.851edyA
Model1
 
40.5312 4.45 0.03 0.861n67A
Model1
 
50.5287 4.36 0.02 0.851ycsA
Model1
 
60.5261 3.94 0.04 0.802rmnA
Model1
 
70.5248 4.63 0.09 0.871imhC
Model1
 
80.5230 4.49 0.06 0.851a02N
Model1
 
90.5199 4.19 0.12 0.813cu7A
Model1
 
100.5171 4.03 0.04 0.791m6uA
Model1
 
Structural alignment using TM-align
 
TPNILRLESEETVVLEAHGGQGTIQVSVTVHDFPAKKQVLSNENTQLNSNNGYLSTVTIKIPASKELKSDKGHKFVTVVATFGNVQVEKVVLISLQSGYLFIQTDKTIYTPGSTVLYRVFTVDHKLLPVGQTVFITIETPDGIPVKRDSKSSQNQFGILTLSWNIPELVNMGVWKIKAYYEDSPQQVFSAEFEVKEYVLPSFEVQLEPEEKFYYIDDPDGLKVNIIARFLYGEQVDGTAFVIFGVQDGDRRISLTHSLTRVPINDGNGEAILKRQVLLNGVQPSRADALVGKSIYVSATVILQSGSDMVEAERTGIPIVTSPYQIHFTKTPKFFKPAMPFDLMVYVTNPDGSPARHIPVVTQGSNVQSLTQDDGVAKLSINTQNKRDPLTITVRTKKDNIPEGRQATRTMQALPYNTQGNSNNYLHLSVPRVELKPGETLNVNFHLRTDPGEQAKIRYYTYMIMNKGKLLKVGRQYREPGQDLVVLPLTITSDFIPSFRLVAYYTLINAKGQREVVADSVWVDVKDSCMGTLVVKNGGKEEKHHRPGQQITLKIEADQGARVGLVAVDKGVFVLNKKNKLTQRKIWDVVEKADIGCTPGSGRNYAGVFTDAGLTLKTSQGLETQQRADPQCPQSVQLMEKRMDKAGQYSSDLRKCCEDGMRDNPMKFPCQRRAQFILQGDACVKAFLDCCEYITQLRQQHSRDGALDDDIIPEEDIISRSQFPESWLWTVIEDLKQADKNGISTKLMNVFLKDSITTWEILAVSLSDKKGICVADPYEVTVMQDFFIDLRLPYSVVRNEQVEIRAILYNYREAENLKVRVELLYNPAFCSLATAKKRHQQTITIPARSSVAVPYVIVPLKIGLHEVEVKAAVYNHFISDGVKKTLKVVPEGVRVNKTVAVRTLNPEHLGQGGVQREEVPAADLSDQVPDTESETKILLQGTPVAQMTEDAIDGERLKHLIQTPSGCGEQNMIGMTPTVIAVHYLDSTDQWEKFGLEKRQESLELIRKGYTQQLAFRQKSSAYAAFQYRPPSTWLTAYVVKVFALAANLIAIDSKDLCETVKWLILEKQKPDGIFQEDGPVIHQEMIGGFRDTREKDVSLTAFVLIALHEAKDICEAQVNSLGRSIAKAGDFLENHYRELRRPYTVAIAAYALALLGKLEGDRLTKFLNTAKEKNRWEEPNQKLYNVEATSYALLALLARKDYDTTPPVVRWLNEQRYYGGGYGSTQATFMVFQALAQYQKDVPDHKELNLDVSIQLPSRNSAVRHRILWESASLLRSEETKENERFTVKAEGKGQGTLSVVTVYHAKLKGKVSCKKFDLRVSIRPAPETVKKP-QDAKGSMILDICTKY--LGDQDATMSILDISM------MTG-FSPDVEDLKTLSTGVDRYISKYEMNRDSNKNTLII---YLD--K-VS---HTVEDCLSFKVHQY--FNVGLIQPGAVKVYSYY--NLDETCIRFYHPDKEDGMLSKLCHKDTCRCAEENCFTLEDRLDKACEPGVDYVYKTRLIQKKLEDDFDEYIMVIENIIKSGSDEVQVKQERKFISHIKCREALKLKEGAHYLVWGVSSDLWGEKPKISYIIGKDTWVELWPEAEECQDEENQKQCEDLANFTENMVVFGCPN 
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------M-S----------------------------------------------------------------------------------------------------------------------------------QVL-FHQLVPLQVKCKDC---EERR-VSIRMSIELQSVSNPVHRKD-LVIRLTDDTDPFFLY-NLVISEEDFQSLKFQ--QGL-LVDFL--------AFPQKFIDLLQQCTQEHAKEIPRFLLQLVSPAAILD--NSPAFLNVVETNPFKH--LTHLSLKLL--P------------------------------------------------------------------------------------------------------------------------------------------------------------ 
 
VQLTEKRMDKVGKYPKELRKCCEDGMRENPMRFSCQRRTRFISLGEACKKVFLDCCNYITELRRQHARALDEDIIAEENIVSRSEFPESWLWNVEDLKEPPKNGISTKLMNIFLKDSITTWEILAVSMSDKKGICVADPFEVTVMQDFFIDLRLPYSVVRNEQVEIRAVLYNYRQNQELKVRVELLHNPAFCSLATTKRRHQQTVTIPPKSSLSVPYVIVPLKTGLQEVEVKAAVYHHFISDGVRKSLKVVPEGIRMNKTVAVRTLDPERLGREGVQKEDIPPADLSDQVPDTESETRILLQGTPVAQMTEDAVDAERLKHLIVTPSGCGEQNMIGMTPTVIAVHYLDETEQWEKFGLEKRQGALELIKKGYTQQLAFRQPSSAFAAFVKRAPSTWLTAYVVKVFSLAVNLIAIDSQVLCGAVKWLILEKQKPDGVFQEDAPVIHQENNNEKDMALTAFVLISLQEAKDICEEQVNSLPGSITKAGDFLEANYMNLQRSYTVAIAGYALAQMGRLKGPLLNKFLTTAKDKNRWEDPGKQLYNVEATSYALLALLQLKDFDFVPPVVRWLNEQRYYGGGYGSTQATFMVFQALAQYQKDAPDHQELNLDVSLQLPSRSSKITHRIHWESASLLRSEETKENEGFTVTAEGKGQGTLSVVTMYHAKAKDQLTCNKFDLKVTIKPAPETEKRP-QDAKNTMILEICTRYR-GDQ-DATMSILDISM------MTG-FAPDTDDLKQLANGVDRYISKYELDKAFSDRNTLII---YLD--K-VS---HSEDDCLAFKVHQY--FNVELIQPGAVKVYAYY--NLEESCTRFYHPEKEDGKLNKLCRDELCRCAEENCFIQKSDDKVTLEERLDKACEPGVDYVYKTRLVKVQLSNDFDEYIMAIEQTIKSGSDEVQVGQQRTFISPIKCREALKLEEKKHYLMWGLSSDFWGEKPNLSYIIGKDTWVEHWPEEDECQDEENQKQCQDLGAFTESMVVFGCPN 
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------M-S-----------------------------------------------------------------------------------------------------------------------------------QVLFHQLVPLQVKCKD-C--EERR-VSIRMSIELQSVSNPVHRKD-LVIRLTDDTDPFFLY-NLVISEEDFQSLK-F-QQGL-LVDFL---------AFPQKFIDLLQQCTQEHAKEIPRFLLQLVSPAAILD--NSPAFLNVVETNPFKH--LTHLSLKLL-P--------------------------------------------------------------------------------------------------------------------------------------------------------------------- 
 
--EAPFTLKVNTLPLNFDKAEHHRKFQIHINVSYI-GERPNSNMVIVDVKM------VSG-FIPVKPSVKKLQDQSNIQRTEVNTNHVLI--Y--IEK--L---TNQTMGFSFAVEQDI-PVKNLKPAPVKVYDYY-ETDEFAIEEYSAPFSSDS 
MSQVLFHQLVPLQVKCKDCEERRVSIRMSIELQSVSNPVHRK-DLVIRLTDDTDPFFLY-NLVISEEDFQSLKFQQGL-LVDFL---AFPQKFIDLLQQCTQEHAKEIPRFLLQLVSPAAILDN-SPAFLNVVETNPFKH-LTHLSLKLLP---- 
 
GTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFKITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLTMPAYIDPENVKKTGNVTLATGIGSTTANKTVLVDYEKYGK--FYNLSIKGTIDQID-K---TNNTYRQTIYVNPSG-DNVIAPVLTGNLKP--NTDSNALIDQQNTSI-KVYKVDNAADLSESYFVNPENFED-VTNSVNITFPNPNQYK---VE--FNT-PDDQ--ITTPYIVVVNGHIDPNSKGDLALRSTLYGYNSNII---WRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEK 
--------------------------------------------------------------------------------------------------------------------------------------------------MSQVLFHQLVPLQVKCKDCEERRVSIRMSIELQSVSNPVHRKDLVIRLTDDTDPFFLYNLVISE-EDFQSL----------------------KFQQ-GLLVDFL---AFPQKFIDLLQQCTQ--EHAKEIPRFLLQLVSPAAILDNSPAFLNVVET-----NPFKHLTHLSLKL------LP------------------------- 
 
VPSQK-TYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQ-----------LAKTCPVQLW-VDSTPPPGTRVRAMAIY-KQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSV--V--VPY--EP-PEVGSDCTTIHYNYMCNSS-CMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEE 
-----MSQV----LFHQ------------------LVPLQVKCKDCEERRVSIRMSIELQSVSNPVHR---KDLVIRLTDDTDP-FF-LYNLV---IS---EEDFQS-------LKFQQGLLVDFL-----AFPQKFIDLLQQCTQEHA--K-EIPRFLLQLVS--PAAIL-DNSPAFLNVVET---NPFKHLTHLSLKLL-P---------- 
 
GSSTFDALSPSPAIPSNT-DYPGPHSFDVSFQQSSTAKSATWTYSTELKKLYCQ----------IAKTCPIQIK--VMTPPPQGAVIRAMPVYKKAEHVTEVVKRCPNHELSREFNEGQIAPPSHLIRVEGNSHAQ-YVEDPITGRQSV---L--VPY--EPPQVGTEFTTVLYNFMCNSS-CVGGMNRRPILIIVTLETRDGQVLGRRCFEARICACPGRDRKADEDSIRKQQVSDSTKNGDAFRQNTHGIQMT 
------------------MSQV----LFHQ------------------LVPLQVKCKDCEERRVSIRMSIELQSVSNPVHRK---DLVIRLTDDTDPFF-LYNLV----I---S-E--EDFQ-----SLKFQQGLLVDFL-------AFPQKFIDLLQQCTQEHA-K-EIPRFLLQLVS--PAAIL-DNSPAFLNVVET---NPFKHLTHLSLKLL-P------------------------------------- 
 
KKSPMLCGQYPVKSEGKELKIVVQPETQHRARY----LTEGSRGSVKDRTQQGFPTV---KLEGHNEPVVLQVFVGNDSGR-VKPHGFYQA---CR-VTGRN--TTPCKEVDIEGTTVI--E--VGLD-PSNNM--TLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPD 
--------------MSQVLFH-QLVP-LQVKCKDCEERRVSIR-MSI------ELQSVSNPVHR----KDLVIRLTDDTDPFFLYN--LVISEEDFQSL---KFQQGLLVDFL---AFPQKFIDLLQQCTQE--HAKEIPRFLLQLVSP--AAI-----L----DNSPAFLNVVETN------PFKHLTHLSLKL--LP------------------------------------------------------------------------------------------------------- 
 
WPLSSQSGSYELRIEVQPKPHHRAHYE----TEGSRGAVKAPTGGHPVVQL-HGYMENKP-LGLQIFIGTADERILKPHAFYQVH--RI-TGKTV--TTTSYEKIVGNTKVL--E--IPL--EP-KNNMRATIDCAGILK-LRNADIELRKGETDIGRKNTRVRLVFRVHIPESSGRIVSLQTASNPIECSQRSAHE 
-------MSQVLFHQ-LVPLQVKCK--DCEERRVSIR-MS-----IELQSVSNPVH----RKDLVIRLTDDTDPFFLYN--LVISEEDFQSL---KFQ-QGLLVDFL--AFPQKFIDLLQQCTQEHAKEIPRFL-LQLVSPAAI--L---------D--NSPAFLNVVETN------PFKHLTHLSLKL-----LP- 
 
EQTYVISAPKIFRVGASENIVIQVYGYTEAFDATISIKSYPDKKFSYSSGHVHLSSENKFQNSAILTIQPKQLPGGQNPVSYVYLEVVSKHFSKSKRMPITYDNGFLFIHTDKPVYTPDQSVKVRVYSLNDDLKPAKRETVLTFIDPEGSEVDMVEEIDHIGIISFPDFKIPSNPRYGMWTIKAKYKEDFSTTGTAYFEVKEYVLPHFSVSIEPEYNFIGYKNFKNFEITIKARYFYNKVVTEADVYITFGIREDLKDDQKEMMQTAMQNTMLINGIAQVTFDSETAVKELSYYSLEDLNNKYLYIAVTVIESTGGFSEEAEIPGIKYVLSPYKLNLVATPLFLKPGIPYPIKVQVKDSLDQLVGGVPVTLNAQTIDVNQETSDLDPSKSVTRVDDGVASFVLNLPSGVTVLEFNVKTDAPDLPEENQAREGYRAIAYSSLSQSYLYIDWTDNHKALLVGEHLNIIVTPKSPYIDKITHYNYLILSKGKIIHFGTREKFSDASYQSINIPVTQNMVPSSRLLVYYIVTGEQTAELVSDSVWLNIEEKCGNQLQVHLSPDADAYSPGQTVSLNMATGMDSWVALAAVDSAVYGVQRGAKKPLERVFQFLEKSDLGCGAGGGLNNANVFHLAGLTFLTNANADDSQENDEPCKEILLQKKIEEIAAKYKHSVVKKCCYDGACVNNDETCEQRAARISLGPRCIKAFTECCVVASQLRANISLGRLHMKTLLPVSKPEIRSYFPESWLWEVHLVPRRKQLQFALPDSLTTWEIQGIGISNTGICVADTVKAKVFKDVFLEMNIPYSVVRGEQIQLKGTVYNYRTSGMQFCVKMSAVEGICTSESPKCVRQKVEGSSSHLVTFTVLPLEIGLHNINFSLETWFGKEILVKTLRVVPEGVKRESYSGVTLDPRGIYGTISRRKEFPYRIPLDLVPKTEIKRILSVKGLLVGEILSAVLSQEGINILTHLPKGSAEAELMSVVPVFYVFHYLETGNHWNIFHSDPLIEKQKLKKKLKEGMLSIMSYRNADYSYSVWKGGSASTWLTAFALRVLGQVNKYVEQNQNSICNSLLWLVENYQLDNGSFKENSQYQPIKLQGTLPVEARENSLYLTAFTVIGIRKAFDICPLVKIDTALIKADNFLLENTLPAQSTFTLAISAYALSLGDKTHPQFRSIVSALKREALVKGNPPIYRFWKDNLQHKDSSVPNTGTARMVETTAYALLTSLNLKDINYVNPVIKWLSEEQRYGGGFYSTQDTINAIEGLTEYSLLVKQLRLSMDIDVSYKHKGALHNYKMTDKNFLGRPVEVLLNDDLIVSTGFGSGLATVHVTTVVHKTSTSEEVCSFYLKIDTQDI-------YKR-IVACASYKPSREESSS-GSSHAVMDISL------PTGISANEEDLKALVEGVDQLFTDYQIKDGHVIL--Q--LNS-IP----SSDFLCVRFRIFEL-FEVGFLSPATFTVYEYH--RPDKQCTMFYSTSNIKIQKVCEGAACKCVEADCGQMQEELDLTISAETRKQTACKPEIAYAYKVSITSITVENVFVKYKATLLDIYKTGEAVAEKDSEITFIKKVTCTNAELVKGRQYLIMGKEALQIKYNFSFRYIYPLDSLTWIEYWPRDTTCSSCQAFLANLDEFAEDIFLNGC 
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------M--S-Q---------------------------------------------------------------------------------------------------------------------------------VLFHQLVPLQVKCKDCEERRVSIRMSIELQSVS----NPVHRKD-LVIRLTDDTDPFFLYNLVISEEDFQSLK-F-QQGL-LVDFL---AFPQKFIDLLQQCTQEHAKEIPRFLLQLVSPAAILD--NSPAFLNVVETNPFKH--LTHLSLKLLP------------------------------------------------------------------------------------------------------------------------------------------------------------------- 
 
FKVGPPFELVRDY-CPVVESHTGRTLDLRIIPRIDRGFDHIDEEWVG----YKRNYFTLVSTFETANCDLDTFLKSSFDLLVEDSSVESRLRVQYFAIKIKAKND-DDDTEINLVQHTAKR--DKGPQF-------CPS---V---C--PLVPSPLPKHQIIREASNVRNITKTKKYDSTFYLHRNHVNYEEYGVDSLLFSYPE-DSI---QKVARYERVQF-ASSISVKKPFQQNKHFSLHVILGAVVDPDTFHGENPGIPYDELALKNGSKGMFVYLQEMKTPPLIIRGRS-- 
-------------MSQV---------LFHQLVPLQVKC---------KDCEERRVSIRMSIELQS----VSNP--V-H--------------R-KDLVIRLTDDTDPFFLYNLVISEED--FQS-----LKFQQGLLVDFLAFPQKFIDLL--------------------------------------------------QQCTQEHAKEIPRFLLQ-LVSPAA-------IL--DNSPAFLNVVETN---------------P---------FKHL-THLSLK-L------LP 
 
(a)Residue pairs that are structurally aligned by TM-align program are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade.(more about the colors used)
(b)Ranking of proteins is based on TM-score of the structural alignment between Model1 and the PDB structures in our template library.
(c)RMSDa is the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.

  Function Prediction

  Predicted EC Numbers

RankTM-scoreRMSDaIDENaCov.EC-ScorePDB
Hit		EC No.
10.4634 4.15 0.12 0.730.56412q3zA 2.3.2.13
 
20.4131 4.60 0.13 0.690.54462i74A 3.5.1.52
 
30.4108 4.87 0.12 0.730.53791u3xA 4.2.3.5
 
40.4123 4.93 0.12 0.760.53721bsfB 2.1.1.45
 
50.4787 4.10 0.10 0.730.52723dypD 3.2.1.23
 
(a)Ranking is based on EC-score, which is a confidence score for the Enzyme Classification (EC) Number prediction.
(b)RMSDa is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)IDENa is percentage sequence identity in the structurally aligned region.
(d)Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)EC-Score is defined based on the C-score of the structure prediction and similarity of the model with known enzyme structures, as identified using both global and local structural alignment programs. The global similarity score uses TM-score, IDENa,RMSDa and Cov. of the structural alignment by TM-align, while the local match compares the structural and chemical similarity of local spatial motifs in the model with known catalytic site of enzymes. A prediction with a EC-score >1.1 signifies a prediction with high confidence (upto 3 digit numbers of EC) and vice versa (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).

  Predicted GO terms

RankTMscoreRMSDaIDENaCov.PDB
Hit		Fh-ScoreAssociated GO Terms
10.5156 4.26 0.13 0.803cu7B 0.48 GO:0004866 GO:0005125 GO:0008009 GO:0002376 GO:0006955 GO:0009987 GO:0032502 GO:0040011 GO:0044237 GO:0050789 GO:0065007 GO:0005576 GO:0005579 GO:0005615 GO:0005623 GO:0005886 GO:0005887 GO:0016021 GO:0046930
20.5200 4.19 0.12 0.813cu7B 0.46 GO:0004866 GO:0005125 GO:0008009 GO:0002376 GO:0006955 GO:0009987 GO:0032502 GO:0040011 GO:0044237 GO:0050789 GO:0065007 GO:0005576 GO:0005579 GO:0005615 GO:0005623 GO:0005886 GO:0005887 GO:0016021 GO:0046930
30.4729 4.37 0.13 0.762g23L 0.44 GO:0005507 GO:0016491 GO:0043169 GO:0009058 GO:0009987 GO:0017000 GO:0044249 GO:0055114
40.5547 4.04 0.10 0.841edyB 0.43 GO:0004867 GO:0005515 GO:0017114 GO:0005576 GO:0005615
50.4178 3.78 0.15 0.631jfqL 0.42 GO:0003823
60.4235 4.43 0.14 0.702bzdC 0.42 GO:0004308 GO:0007155 GO:0008152 GO:0022610 GO:0005576
70.4475 3.61 0.14 0.671tzoO 0.42 GO:0005509 GO:0005515 GO:0043169 GO:0009405 GO:0005576
80.3491 4.87 0.16 0.642r6gB 0.41 GO:0005351 GO:0005402 GO:0005524 GO:0015154 GO:0015293 GO:0015405 GO:0015422 GO:0015423 GO:0015608 GO:0015609 GO:0022892 GO:0030554 GO:0042626 GO:0043492 GO:0015766 GO:0015768 GO:0005623 GO:0005886 GO:0043190 GO:0044425
90.4598 4.08 0.13 0.691j8hD 0.41 GO:0003823 GO:0004872 GO:0042287 GO:0042605 GO:0002376 GO:0006955 GO:0005623 GO:0005886
100.5200 4.10 0.10 0.801ayoB 0.41 GO:0004867 GO:0005515 GO:0005576

Consensus Prediction of Gene Ontology terms 
Molecular Function  Biological Process Cellular Location
GO termGO-Score  GO termGO-Score GO termGO-Score
GO:00054880.389  GO:00069550.309  GO:00055760.262
GO:00055150.261  GO:00099870.301  GO:00056230.258
GO:00302340.178  GO:00081520.261  GO:00444640.258
GO:00048570.178  GO:00442370.219  GO:00160200.218
GO:00304140.178  GO:00508960.216  GO:00058860.218
GO:00048660.178  GO:00650070.215  GO:00329910.176
GO:00051020.135  GO:00325020.175  GO:00432340.176
GO:00038240.127  GO:00507890.175  GO:00444210.137
GO:00080090.094  GO:00023760.175  GO:00056150.137
GO:00423790.094  GO:00226100.123  GO:00444250.135

(a)Ranking in the first table is based on a function prediction score (Fh-score), which is calculated based on the C-score of the structure prediction and the TM-score, IDENa, RMSDa and Cov. of the structural alignment by TM-align between the predicted model and the PDB structures (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).
(b)RMSDa is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)IDENa is the percentage sequence identity in the structurally aligned region.
(d)Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)A consensus prediction of GO terms is derived from the structural analogs that have an Fh-score of >=1.0. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on the Fh-score of the template from which the GO term is derived. A prediction with a GO-score >0.5 signifies a prediction with high confidence and vice versa.


  Predicted Binding Site


Binding site residues in the model:
VAL:4  LEU:5  PHE:6  HIS:7  GLN:8  LEU:9  VAL:10  
SER:29  ILE:30  GLU:31  LEU:32  GLN:33  SER:34  VAL:35  
SER:36  PRO:38  VAL:39  HIS:40  ARG:41  LYS:42  ASP:43  
LEU:44  VAL:45  ILE:46  ARG:47  LEU:48  THR:49  ASP:50  
LEU:91  GLN:92  CYS:94  THR:95  GLN:96  HIS:98  LYS:100  
GLU:101  ILE:102  PRO:103  ARG:104  PHE:105  LEU:107  ALA:121  
PHE:122  LEU:123  ASN:124  VAL:125  VAL:126  GLU:127  THR:135  
HIS:136  LEU:137  SER:138  LEU:139  
Identified analogs with similar binding site:

RankPDB
Hit		TM-scoreRMSDaIDENaCov.BS-scoreDownload
Complex		Binding site residues on the predicted model
12e1qA0.3738 5.34 0.10 0.68 0.40Download4,5,6,7,8,9,10,29,30,31,32,33,34,35,36,38,39,40,41,42,43,44,45,46,47,48,49,50,91,92,94,95,96,98,100,101,102,103,104,105,107,121,122,123,124,125,126,127,135,136,137,138,139
21um4H0.4527 4.44 0.07 0.70 0.39Download45,47,49,80,81,83,84,85,86,87,88,89,124,126,128,129,130,131,132,134
31qd0A0.4743 3.75 0.07 0.68 0.38Download34,35,36,37,38,39,40,41,42,43,45,83,84,85,86,87,88,89,90,91,92,93,126,127,128,129,130
41fhjB0.3343 4.32 0.15 0.52 0.35Download31,46,70,71,72,73,74,75,76,77,78,83,86,87,90,91,94,98,102,103,104,105
52h12B0.3944 4.83 0.09 0.69 0.33Download6,7,8,9,30,41,44,123,125,136,137,138
61tjjA0.4173 5.58 0.08 0.81 0.32Download6,8,30,31,32,33,43,44,45,46,47,48,49,60,61,62,63,67,70,75,76,77,78,79,80,83,87,91,103,104,105,106,107,108,123,124,125,126,135,136,137
71wz1H0.4605 3.72 0.08 0.67 0.32Download3,4,5,6,39,40,41,42,43,44,45,125,126,127,128,132,133,134
81qpwB0.3342 4.35 0.14 0.52 0.30Download31,46,70,71,72,73,74,75,76,77,78,83,87,90,91,94,98,103,104,105
91tjjA0.4173 5.58 0.08 0.81 0.30Download10,12,26,28,29,30,31,32,46,104,105,106,107,117,118,119,120,121,123,125,139,140,141
102r1hB0.3289 4.27 0.16 0.51 0.29Download31,67,70,71,72,73,74,75,76,77,78,83,87,90,94,97,98,103,104,105

(a)Ranking of the analogs in the table is based on their BS-score. BS-score is calculated by first finding the structural analogs of the predicted model based on their TM-score, IDENa and Cov. of the structural alignment. The ligand(s) in the analog structure are then transferred onto the model and the fitness of the ligand-model complex (BS-score) is calculated by comparing the local structure and sequence similarity in the binding site region. (For detail, see Kucukural, Szilagyi, Ambrish, and Zhang, Template based ligand binding site prediction on modeled protein structure, 2009, in preparation).
(b) A BS-score of >0.5 signifies a binding site prediction with high confidence and vice-versa.
(c)RMSDa the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.
(f)The image shows the ligand-protein complex with the best BS-score. The ligand is depicted in magenta colored ball & stick, the predicted binding site residues interacting with the ligand are shown as transparent green spheres, while the N & C terminus in the model are marked by blue and red spheres respectively.



 Please cite following articles when you use the I-TASSER server:
1.Yang Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9:40 (2008).
2.Yang Zhang. Template-based modeling and free modeling by I-TASSER in CASP7. Proteins, 8: 108-117 (2007).
3.Sitao Wu, Jeffrey Skolnick, Yang Zhang. Ab initio modeling of small proteins by iterative TASSER simulations. BMC Biology, 5:17 (2007).