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I-TASSER results for job id S34207

  Submitted Sequence

>myo1B41
NKKQILIRVYLLNEIPITVGVESYTSVKMLKQELLQKIGITQRHEYYGLMEVRNNIKKKR
KETRFIDESERVMDVIAFWENEQYLFGSQLNSSFIHSNSKQNDEGIEQFNLQNYQDLEGL
KIYLGIRILFSFQENDKEAISVFYTQLSSEVLEGKYAPLEEKDYLNLAALQLCIDFPRDV
ILKEISKRQSINADSGSPQRTQSNFLSATSQSESGASQLKKLSYQDIEQIDEQDEYEQFI
QNRNEILIDPVFYIPAKMYFQKDIQQWQHLILQNLVDNRLLKKEYTPSQAKLAYILYLSK
QDLFMSTIF

  Predicted Secondary Structure

Sequence                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300
                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |          
NKKQILIRVYLLNEIPITVGVESYTSVKMLKQELLQKIGITQRHEYYGLMEVRNNIKKKRKETRFIDESERVMDVIAFWENEQYLFGSQLNSSFIHSNSKQNDEGIEQFNLQNYQDLEGLKIYLGIRILFSFQENDKEAISVFYTQLSSEVLEGKYAPLEEKDYLNLAALQLCIDFPRDVILKEISKRQSINADSGSPQRTQSNFLSATSQSESGASQLKKLSYQDIEQIDEQDEYEQFIQNRNEILIDPVFYIPAKMYFQKDIQQWQHLILQNLVDNRLLKKEYTPSQAKLAYILYLSKQDLFMSTIF
PredictionCCCCCSSSSSSSCCCSSSSSSCCCCCHHHHHHHHHHHHCCHHHHCCSSSSSSSCCCCCCSSSSSSCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCSSSSSSHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCSSSCCCSSCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHCHHHHHHHHCC
Conf.Score997725999996796799982475779999999998848742114268999961556512454322756889999999888887640112212010344412101146666667642272467651422675559947899989999999846885688588999999999988605321544445115615566994134657887866753025665443340186641134799998445625432412211888888789999999999887767764126801544566520104455665249

  Top 5 Models predicted by I-TASSER

Download Model 1 Download Model 2 Download Model 3 Download Model 4 Download Model 5
C-score=-1.38
 C-score=-1.60
 C-score=-3.97
 C-score=-3.80
 C-score=-4.72
 
Estimated accuracy of Model1: 0.54±0.15 (TM-score)    9.4±4.6Å (RMSD)    (Read more about C-score of generated models)

  Top 10 templates used by I-TASSER

RankPDB
Hit		Iden1Iden2Cov.Norm.
Z-score		Download
Align.		                   20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300
                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |         
Sec.Str
Seq		CCCCCSSSSSSSCCCSSSSSSCCCCCHHHHHHHHHHHHCCHHHHCCSSSSSSSCCCCCCSSSSSSCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCSSSSSSHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCSSSCCCSSCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHCHHHHHHHHCC
NKKQILIRVYLLNEIPITVGVESYTSVKMLKQELLQKIGITQRHEYYGLMEVRNNIKKKRKETRFIDESERVMDVIAFWENEQYLFGSQLNSSFIHSNSKQNDEGIEQFNLQNYQDLEGLKIYLGIRILFSFQENDKEAISVFYTQLSSEVLEGKYAPLEEKDYLNLAALQLCIDFPRDVILKEISKRQSINADSGSPQRTQSNFLSATSQSESGASQLKKLSYQDIEQIDEQDEYEQFIQNRNEILIDPVFYIPAKMYFQKDIQQWQHLILQNLVDNRLLKKEYTPSQAKLAYILYLSKQDLFMSTIF
11h4rA 0.15 0.20 0.80 0.92Download --KTFTVRIVTMDA-EMEFNCEMKWKGKDLFDLVCRTLGLRE-TWFFGLQYTIKD------TVAWLKM------------------DKKVLDHDV-------------------SKEEPVTFHFLAKFYPENAEVQEITQHLFFLQVKKQILDEKI-YCPPEASVLLASYAVQAKYGDYD--PSVHKRGFLAQEELLPKRVINL-------YQMTPEMWEERITAWYAEHARDEAEMEYLKIRNKHIYDPENRLTPKISFPNEIRNISYFTIK-PLDK---KIDVSSKLRVNKLILQLCNHDLFMRRRK
22he7A 0.13 0.17 0.62 4.23Download --KSMQCKVILLDGSEYTCDVEKRSRGQVLFDKVCEHLNLLEK-DYFGLTYRDAE-----NQKNWLDPAKEIK--------------------------------------KQV-RSGAWHFSFNVKFYPPDPASEDITRYYLCLQLRDDIVSGRL-PCSFVTLALLGSYTVQSELGDYDP------------DECG-----SDYI------------------------------------------SEFRFAPNH------TKELEDKVIELHKS----HRGMTPAEAEMHFLENAKKLSMYGVDL-
31h4rA 0.13 0.20 0.84 1.61Download --KTFTVRIVTMDA-EMEFNCEMKWKGKDLFDLVCRTLGLRE-TWFFGLQYTIKD------TVAWLKMDKKVLD-----------HDVSK--------------------------EEPVTFHFLAKFYPENELVQEITQHLFFLQVKKQILDEKI-YCPPEASVLLASYAVQAKYGDSVHKRGFLAQEELLPKRNLYQMTPEMWEERITAWYAERDEAEMEYLKIAQDIRNKKGTELLLGVDALHIYDPENLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVKLRVNKLILQLCIGNHDLFMRRRK
41e5wA 0.11 0.21 0.85 1.46Download MPKTISVRVTTMDAE-LEFAIQPNTTGKQLFDQVVKTIGLREVW-FFGLQYQDTKGF-----STWLKLNKKVTA-------------------------------------QDVRKESPLLFKFRAKFYPEDELIQDITQRLFFLQVKEGILNDDI-YCPPETAVLLASYAVQSKYGDFNKEVHKSGQRVLEQHKLNKDQWEERIQVWHEEHRGMLREDAVLEYLKIAQDLEMFVIKPIDKKAPDFVFYAPRLRINKRILALCEVQQMKAQAREEKHQKQMERAMLENEKKKREMAEKEKEKIEREKEE
51h4rA 0.14 0.20 0.83 0.97Download --KTFTVRIVTMDA-EMEFNCEMKWKGKDLFDLVCRTLGLRE-TWFFGLQYTIKD------TVAWLKM------------------DKKVLDHDVSK-------------------EEPVTFHFLAKFYPENAEVQEITQHLFFLQVKKQILDEKI-YCPPEASVLLASYAVQAKYGDYDP--SVHKRGFLAQEELLPKRVINL-YQMTPEMWEERITAWYAEHRGRARDEAEMEYLKIAQDLEMHIYDPENRLTPKISFPNEIRNISYSDKEFTIKPLDKKIDVKLRVNKLILQLCIGNHDLFMRRRK
61h4rA 0.13 0.20 0.83 3.99Download --KTFTVRIVTMDAEM-EFNCEMKWKGKDLFDLVCRTLGLRE-TWFFGLQYTIK------DTVAWLKMDKKVLDHDV-------------------------------------SKEEPVTFHFLAKFYPENELVQEITQHLFFLQVKKQILDEKI-YCPPEASVLLASYAVQAKYGDYDPSVHKLAQEELLPKRVINQMTPEMWEERITAWEAEMEYLKIAQDLEMYGVNYFAIRNKKGTELGLHIYDPENRLPKISFPWNEIRNISTIKPLDKKID---VFKFNSSKNKLILQLCIGNHDLFMRRRK
72al6A 0.14 0.17 0.87 0.70Download MERVLKVFHYFENSSEPTIRHGDATDVRGIIQKIVDCHKVKNVACYGLRLSHLQS-----EEVHWLHLDMGVSNVREKFELAH--------------------------------PPEEWKYELRIRYLLNQFTEDKPTLNFFYQQVKNDYMLEIADQVDQEIALKLGCLEIRRSYGE---MRGNALEKKSNYEVLEKDVGLRRFFPKSLLDSVKAKTLRKLIQQTFRQLNREESILKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGISYLTDKGANPTHLADFNQVQTIQYSNSEDKDRK
81h4rA 0.12 0.20 0.83 4.04Download --KTFTVRIVTMDAEME-FNCEMKWKGKDLFDLVCRTLGLR-ETWFFGLQYTIKD------TVAWLKMDKKVLD-------------------------------------HDVSKEEPVTFHFLAKFYPENELVQEITQHLFFLQVKKQILDEKI-YCPPEASVLLASYAVQAKYGDYDPSVHKRGQEELLPKRVINLMTPEMWEERITAWYAEMEYLKIAQDLEMYGVNYFAIRNKKGTELLLHIYDPENRLTPKSFPWNEIRNISTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFM----
91h4rA 0.13 0.20 0.84 1.45Download --KTFTVRIVTMDA-EMEFNCEMKWKGKDLFDLVCRTLGLRETW-FFGLQYTIKD------TVAWLKMDKKVLDHDVSKE-------------------------------------EPVTFHFLAKFYPENELVQEITQHLFFLQVKKQILDEKI-YCPPEASVLLASYAVQAKYGDSVHKRGFLAQEELLPKRNLYQMTPEMWEERITAWYAERDEAEMEYLKIAQDIRNKKGTELLLGVDALIYDPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVKLRVNKLILQLCIGNHDLFMRRRK
101gg3A 0.11 0.20 0.81 0.90Download ----MHCKVSLLDDTVYECVVEKHAKGQDLLKRVCEHLNLLE-EDYFGLAIWDNATSKT------------WLD------------SAKEIKKQVR--------------------GVPWNFTFNVKFYPPDPATEDITRYYLCLQLRQDIVAGRL-PCSFATLALLGSYTIQSELGDYD--PELHGVDYVSDFKLAPNQT-KELEEKVMELHKRSMTPAQADLEFLENAKKLSMYDLHKAKDLELGVCSLLVYK-RINFPPKVLKISYFFIKIRPGEQEQYESFSYRAAKKLWKVCVEHHTFFR----
(a)All the residues are colored in black; however, those residues in template which are identical to the residue in the query sequence are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade. (more about the colors used)
(b)Rank of templates represents the top ten threading templates used by I-TASSER.
(c)Ident1 is the percentage sequence identity of the templates in the threading aligned region with the query sequence.
(d)Ident2 is the percentage sequence identity of the whole template chains with query sequence.
(e)Cov. represents the coverage of the threading alignment and is equal to the number of aligned residues divided by the length of query protein.
(f)Norm. Z-score is the normalized Z-score of the threading alignments. Alignment with a Normalized Z-score >1 mean a good alignment and vice versa.
(g)Download Align. provides the 3D structure of the aligned regions of the threading templates.
(h)The top 10 alignments reported above (in order of their ranking) are from the following threading programs:
       1: MUSTER   2: HHSEARCH   3: SP3   4: PROSPECT2   5: PPA-I   6: HHSEARCH I   7: FUGUE   8: HHSEARCH II   9: SPARKS   10: MUSTER   

  10 proteins in PDB which are structurally closest to the first I-TASSER model (identified by TM-align)

RankTM-scoreRMSDaIDENaCov.PDB
Hit
10.7377 2.66 0.11 0.821h4rA
Model1
 
20.7295 2.90 0.10 0.831e5wA
Model1
 
30.7295 2.88 0.10 0.832i1jA
Model1
 
40.6854 3.05 0.09 0.791gg3A
Model1
 
50.6676 3.74 0.12 0.832j0jA
Model1
 
60.6605 4.17 0.12 0.852aehA
Model1
 
70.4552 6.21 0.05 0.762va8A
Model1
 
80.4197 6.68 0.06 0.741w6jA
Model1
 
90.4123 5.71 0.07 0.641pxyA
Model1
 
100.4109 6.55 0.12 0.701d8cA
Model1
 
Structural alignment using TM-align
 
-K-TFTVRIVTMD-AEMEFNCEMKWKGKDLFDLVCRTLGLRET-WFFGLQYTIKD------TVAWLKMDKKVLDHD-------------------------------------VSKEEPVTFHFLAKFYPENAEEELVQEITQHLFFLQVKKQILDEKI-YCPPEASVLLASYAVQAKYGDYDPSVHKRGFLAQEELLPKRVINLYQMTPEMWEERITAWYAEHRGRARDEAEMEYLK-IAQDL--EMYGVNYFAIRNKKGTELLLGVDALGLHIYDPENRLTPKISFPWNEIRNISYSDK--EFTIKPLDKKIDVFKF--NSSKLRVNKLILQLCIGNHDLFMRRRKA 
NKKQILIRVYLLNEIPITVGVESYTSVKMLKQELLQKIGITQRHEYYGLMEVRNNIKKKRKETRFIDESERVMDVIAFWENEQYLFGSQLNSSFIHSNSKQNDEGIEQFNLQNYQDLEGLKIYLGIRILFSF---QENDKEAISVFYTQLSSEVLEGKYAPLEEKDYLNLAALQLCIDFPRDVILKEISKRQS-INAD-S-GSP--QRTQSNFLSATSQSE-SG--A---SQLKKLSYQDIEQIDEQDEYEQFIQ--------NRN-------EILIDPVFYIPAKMYFQKDIQQWQH---LILQNLV-------DNRLLKKEYTPSQAKLAYILYLSKQDLFMSTIF- 
 
MPKTISVRVTTMD-AELEFAIQPNTTGKQLFDQVVKTIGLREV-WFFGLQYQDTKG-----FSTWLKLNKKVTAQD---V--------------------------------R--KESPLLFKFRAKFYPEDVSEELIQDITQRLFFLQVKEGILNDDI-YCPPETAVLLASYAVQSKYGDFNKEVHKSGYLAGDKLLPQRVLEQHKLNKDQWEERIQVWHEEHRGMLREDAVLEYLK-IAQDL--EMYGVNYFSIKNKKGSELWLGVDALGLNIYEQNDRLTPKIGFPWSEIR---NISFNDKKFVIKPIDKKAPDFV-FYAPRLRINKRILALCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQMERAMLENEKKKREMAEKEKEKIEREKEE 
NKKQILIRVYLLNEIPITVGVESYTSVKMLKQELLQKIGITQRHEYYGLMEVRNNIKKKRKETRFIDESERVMDVIAFWENEQYLFGSQLNSSFIHSNSKQNDEGIEQFNLQNYQDLEGLKIYLGIRILFSF---QENDKEAISVFYTQLSSEVLEGKYAPLEEKDYLNLAALQLCIDFPRDVILKEISKRQS-INAD-S-GSPQRT--QSNFLSATSQSES-G--A---SQLKKLSYQDIEQIDEQDEYEQFI---------QNRN-----EILIDP-VFYIPAKMYFQKDIQQWQHLILQ--NLVD--------NRLLKKEYTPSQAKLAYILYLSKQDLFMSTIF-------------------------------------------------- 
 
--KSMNVRVTTMD-AELEFAIQQTTTGKQLFDQVVKTIGLRE-VWFFGLQYTDSKG-----DLTWIKLYKKVMQQD-------------------------------------VKKENPLQFKFRAKFYPEDVADELIQEITLKLFYLQVKNAILSDEI-YCPPETSVLLASYAVQARHGDHNPAVHGPGFLANDRLLPQRVTDQHKMSREEWEQSITNWWQEHRGMLREDAMMEY-LKIAQDL--EMYGVNYFEIRNKKNTELWLGVDALGLNIYEKDDKLTPKIGFPWSE-IRNISFNDRKFIIKPIDKKAPDFVFF---APRVRVNKRILALCMGNHELYMRRRKPDTIDVQQMKAQAREEKLAKQAQREKLQLEIAARERAEKKQQEYQDRLRQMQEEMERSQANLLEAQDMVEDARRKQDEAAAALLAATTPQHHHVAERESGGGDLARGPDDLVDPVADRRTLAERNERLHNQLKALKQDLARSCDETKETAMDKIHRENVRQGRDKYKTLREIRKGNTKRRVDQFENM 
NKKQILIRVYLLNEIPITVGVESYTSVKMLKQELLQKIGITQRHEYYGLMEVRNNIKKKRKETRFIDESERVMDVIAFWENEQYLFGSQLNSSFIHSNSKQNDEGIEQFNLQNYQDLEGLKIYLGIRILFS-F--QENDKEAISVFYTQLSSEVLEGKYAPLEEKDYLNLAALQLCIDFPRDVILKEISKRQS-INAD-S-GSP-QRT-QSNFLSATSQSES-G--A---SQLKKLSYQDIEQIDEQDEYEQFIQ--------NRN-------EILIDPVFYIPAKMYFQKDIQQWQHL---ILQNL-------VDNRLLKKEYTPSQAKLAYILYLSKQDLFMSTIF------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 
 
----MHCKVSLLDDTVYECVVEKHAKGQDLLKRVCEHLNLLEEDYFGLAIWDN-A------TSKTWLDSAKEIKKQVR---------------------------------G-V-P----WNFTFNVKFYPPDPAQLTEDITRYYLCLQLRQDIVAGRLP-CSFATLALLGSYTIQSELGDYDPELHGVDYVSDFKLAPNQTKELEEKVMELHKSYRSMTPAQADLEF-LENAKKL--SMYGVDLHKAKDLEGVDIILGVCSSGLLVYK-DK-LRINRFPWPK-VLKISYKRSSFFIKIRPGEQEQYESTIGF--KLPSYRAAKKLWKVCVEHHTFFR---- 
NKKQILIRVYLLNEIPITVGVESYTSV-KMLKQELLQKIGITQRHEYYGLMEVRNNIKKKRKETRFIDESERVMDVIAFWENEQYLFGSQLNSSFIHSNSKQNDEGIEQFNLQNYQDLEGLKIYLGIRILFSF--QENDKEAISVFYTQLSSEVLEGKYAPLEEKDYLNLAALQLCIDFPRDVILKEISKRQSINADSGSPQRTQSNFLSATSQSESGA---SQLKKLSYQDIEQIDEQDEYEQFIQ--------NRN------EILIDPVFYIPAKMYFQKDIQQWQHL----ILQNL---------VDNRLLKKEYTPSQAKLAYILYLSKQDLFMSTIF 
 
----RVLKVFHYFENSSEPTTWASIIRHGDATDVRGIIQKIVDC-HKVKNVACYGLRLSHLQSE---EV-HWLHLDMGVSNVREK-F--EL----------------------AHP-PEEW------KYELRIRYLPKGFLNQFTEDKPTLNFFYQQVKNDYMLEIADQVDQEIALKLGCLEIRRSYGEMRGNALEKKSNYEVLEKDVGLRRFFPKSLLDSVKAKTLRKLIQQTFRQFANLNREESILKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGISYLTD--K-GANPTHLADFNQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLTV--TAPSLTIAENMADLIDGYCRLVNGATQSFIIRPTDDYAEIIDEEDTYTMPSTRDYEIQRERIELGRCIGEGQFGDVHQGIYMSPENPAMAVAIKTCKNCTSDSVREKFLQEALTMRQFDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQVRKFSLDLASLILYAYQLSTALAYLESKRFVHRDIAARNVLVSSNDCVKLGDFGLSRYMEDLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILEEEKLQ--- 
NKKQILIRVYLL-N----E--IPITVGVESYTSVKMLKQELLQKIGITQRHEYYGLMEVRNNIKKKRKETRFIDESERVMDVIAFWENEQYLFGSQLNSSFIHSNSKQNDEGIEQFNLQNYQDLEGLKIYLGIRILF-SF--QE-NDKEAISVFYTQLSSEVLEGKYAPLEEKDYLNLAALQLCIDFPRDVILKE----ISKRQS----IN-ADSGSPQ--RTQ--S-NFLSATSQSESGASQLKKLSYQDIEQIDEQDEYEQFIQN--------RNEI---------LID-PVFYIPAKMYFQKDIQQWQHL------------ILQNL----VDNRLLKKEYTPSQAKLAYILYLSKQDLFMS----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TIF 
 
-M-ERVLKVFHYFENSSEPTTWASIIRHGDATDVRGIIQKIVDC-HKVKNVACYGLRLSHLQSE---EVHWL-HLDMGVSNVRE--K---------------F-E--L----AHP--PEEW------KYELRIRYLPKGFLNQFTEDKPTLNFFYQQVKNDYMLEIADQVDQEIALKLGCLEIRRSYGEMRGNALEKKSNYEVLEKDVGLRRFFPKSLLDSVKAKTLRKLIQQTFRQFANLNREESILKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGISYLT-DKGANPTHLADFNQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLT-VT---APSLTIAENMADLIDGYCRLVNGATQSFIIRPQ--- 
NKKQILIRVYLL-N----E--IPITVGVESYTSVKMLKQELLQKIGITQRHEYYGLMEVRNNIKKKRKETRFIDESERVMDVIAFWENEQYLFGSQLNSSFIHSNSKQNDEGIEQFNLQNYQDLEGLKIYLGIRILF-SF--QE-NDKEAISVFYTQLSSEVLEGKYAPLEEKDYLNLAALQLCIDFP-RD--VILKE-ISKRQS----IN-ADSGSP-QRT-Q--S-NFLSATSQSESGASQLKKLSYQDIEQIDEQDEYEQFIQN--------RNEILI-------DP-VFYIPAKMYFQKDIQQWQHL------------ILQ------NLVDNRLLKKEYTPSQAKLAYILYLSKQDLFMS----------TIF 
 
EWMPIEDLKLPSNVIEIIKKRGIKKLNPPQTEAVKKGLLEGNRLLLTSPTGSGKTLIAEMGIISFLLKNGGKAIYVTPLRA--LTNEKYLTF------KDWELIGF----KVAMTSGDYDTDDAWLKNYDIII----TTYEKLDSLWRHRPEWLNEVNYFVLD-ELHYLNDPERGPVVESVTIRAKRRNLLALSATISNYKQIAKWLGAEPVATNWRPVPLIEGVIYPERKKKEYNVIFKDNTTKKVHGDDAIIAYTLDSLSKNGQVLVFR--NSRKMAESTALKIANYMNFVSLDENALSEILKQLDDIEEGGSDEKELLKSLISKGVAYHHAGLSKALRDL-IEEGFRQRKIKV-I-V-ATPTLAAGVNLPARTVIIGDIPIMEYKQMSGRAGRPGFDQIGESIVVVRDKEDVDRVFKKYVLSDVEPIES--------------------------------KLGS------E-RAFYTFLLGILSAEGNLSE-KQLENFAYESLLAKQLVDVYFDRAIRWLLEHSFIKEEGNTFALTNFGKRVADLYINPFTADIIRKGLEGHKASCELAYLHLLAFTPDGPLVSVGRNEEEELIELLEDLDCELLIEEPYEEDEYSLYINALKVALIMKDWMDEVDEDTILSKY---N---------IGSGDLRNMVETMDWLTYSAYHLSRELKLNEHADKLRILNLRVRDGIKEELLELVQISGVGRKRARLLYNNGIKELGDVVMNPDKVKNLLGQKLGEKVVQEAARLLN 
-------------------NKKQ---------------------------I-LIR-------------------VYLLN--EIPI-TVGVE-SYTSVKML------KQELLQKIGITQR--------------HEYYGL------------------------ME--V---------------------------------------------------------------------------------------------------RNNIKKKRK--------------------------------------------------------ETRFIDESERVMDVIAFWENEQYLFGSQLNSSFIHSN-------SKQNDE--GIEQFNLQNYQDLE----------------------------------GLKIYLGIRILFSFQENDKEAISVFYTQLSSEVLEGKYAPLEEKDYLNLAALQLCIDFPRDVILKEISKRQSINADS-GSPQRTQSNFLSATS----QS-----ESGAS-QLKKL-S-YQD-IE--Q-----------------IDEQDEYEQFIQNR--NE-ILI-----------------DPV-FYI--PAK-MYFQKDI-Q-QWQHLILQNLVDNRLLKKEYTPSQAKLAYILYLS-K----------------------------QDLFMS-T--I--F---------------------------------------------------- 
 
CLRRRGGPYKTEPATDLGRWRLNCERGRQTWTYLQDERAGREQTGLEAYALGLDTKNYFKDLPKAHTAFEGALNGMTFYVGLQAEDGHWTGDYGGPLFLLPGLLITCHVARIPLPAGYREEIVRYLRSVQLPDGGWGLHIEDKSTVFGTALNYVSLRILGVGPDDPDLVRARNILHKKGGAVAIPSWGKFWLAVLNVYSWEGLNTLFPEMWLFPDWAPAHPSTL-------------------------------------------------------WCHCRQVYLPMSYCYAVRLSAAEDPLVQSLRQELYVEDFASIDWLAQRNNVAPDELYTPHSWLLRVVYALLNLYEHHHSAHLRQRAVQKLYEHIVADDRFTKSISIGPISKTINMLVRWYVDGPASTAFQEHVSRIPDYLWMGLDGMKMQGTNGSQIWDTAFAIQALLEAGGHHRPEFSSCLQKAHEFLRL-SQVPDNPPDYQKYYRQMRKGG-------------FSFSTLDCGWIVSD--CTAEALKAVLLLQEKCPHVTEHIPRERLCDAVAVLLNMRNPDGGFATYETKRGGHLLELLNPSEVFGDIMIDYTYVECTSAVMQALKYFHKRFPEHRAAEIRETL-----------------TQGLEFCRR-Q-Q-RADGSWEGSWGVCFTYGTWFGLEA---------FAC--MGQTY----------------------------------------RDGTACAEVSRACDFLLSRQMADG--GWGED---FESCEERRYLQSAQSQIHNTCWAMMGLMAVRHPDIEAQERGVRCLLEKQLPNGDWPQ--ENIAGVFNKSC---AIS--YTSYRNIFPIWALGRFSQLYPERALAGHP 
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NKKQILIRVYLLNEIPITVGVESYTSVKMLKQELLQKIGITQRHEYYGLMEVRNNIKK---------------------------------------------------------------------------------------------------------------------------------------------------KR--K--ET-RF-ID--E----S--------ER-VMDVIAFWEN-------------------EQYLFGSQLNSSFIHSNSKQNDEGI--EQFNLQNY----------------Q--DLEG-LK------------------------------------------------IYLGI--------------------RILFSFQENDKEAISVFYTQLSSEVLEGKYAPLEE--------------------KDYLNLAALQ--LCID---FPRDVILKEISKRQSINADSGSPQRTQSNFLSATSQSESGASQLKKLSYQDIEQIDEQDEY---EQFIQNRNEILIDP--VFYIPA-KMY---F--------------------Q-KDIQQWQHL-ILQNLVDNRL--------LKKEYTPSQAKLAYILYLSKQDLFM-ST--IF----- 
 
SEKGP--FVQHI-----------NRYLGDDPFLKQFLPLDPHSNQLYELVKDGVLLCKLINVAVP--------G---T-----------------IDERA----I---N-TK-RVLNPWERNENHTLCLNSAKAVGCSVVNIGT-QD-LAEGR-PHLVL-GLISQLIKIQLLADLNLKKLRLPPEKVLLKWMNFHLKKGGYKKTVSNFSADLKDAQAYAFLLNVLAPEHCDPATLDAKDPLERAELVLSHAERMNCKRYLTAEEIVEGSSTLNLAFVAQIFHERNGLN-------DVETC--RDERCYRLWINSLGIDSY-------------VN-NV-FEDVR-N----------GWILLEVLDKVSPSSVNWKHASKPPIKMPFRKVENCNQVIKIGKQLKFSLVNVAGNDIVQGNKKLIL-GLLWQLMRFHMLQL--L-----KSLRSEMTDADILSWANRKVRTMGRKLQIESFKDKSLSSGLFFLNLLWA-VEPRVVNWNLVTKGETDDEKRLNATYIVS-----------VARKLGCSVF-LLPEDIVEVNQKMILILTASIMYWSLQR 
-----NKKQILIRVYLLNEIPITVGVESYTS-------------------------VKMLKQELLQKIGITQRHEYYGLMEVRNNIKKKRKETRFIDESERVMDVIAFWENEQYLFGSQLNSSFIH--S------------NSKQNDEGIEQFNLQNYQDLEGLKIY-L-----------------------------------------------------------------------------------------------------------------------GIRILFSFQENDKEAISVFYTQLSSEVLEG--KYAPLEEKDYLNLA-ALQLCIDFPRDVILKEISKRQSINADS-GS----PQRTQSNFL--------S--ATSQ-----------------SES-GASQLKKLSYQDIEQIDEQDEYEQFIQNRNEILIDPVFYIPAKMYFQKD--I-------Q-------------QWQHLILQNLVD--------------------------NRLLKKEYTPSQAKLAYILY-LSKQDLFMSTIF----------------- 
 
QTITQSRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFD-------EIVHD-------LA-PENRQ-LLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVETTGIDSEITSQAGPQLV-----VPANARYALNAANARWGSLYDALYGSDIIPQEGAVSGYDPQRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVGYRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVAAV-------DA---E---------D---KILLY--RNLLGLQG-TLQRKLNDDRHYTAADGSEISLHGRSLLF--IRNVGHLTIP-VIWDSEGNEIPEGILDGVTGAIALYDLKVQKNSRTGSVYIVKPK-------HGPQEVAFANKLFTRIETLGAPNTLKGIDEERRTSLNLRSCIAQARNRVA-FINTGFLDRTGDEHSVEAGPLRKNQ-KSTPWIKAYERNNVLSGLF-C------------GLRGKAQIGKGWAPDLADYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQT-EFNAEFEPLLDDLLTIPVAENANWSAQEIQQ-----ELDN---NVQ-GILGYVVRWVEQGIGCSKVPDIHNVALEDR-ATLRI---SSQHIANWLRHGILTKEQVQA-SLENAKVVDQQNAGDPAYRPAGNFAN---SC-AFKAASDLIFLGVKQPNGYTEPLLHAWRLREKES-- 
--NKK--Q-I-----------------------LIRV-YLLNEIPITVGVESYTSVKMLKQELLQKIGITQRHEY---------------------------------------------------------YGLMEVRNN---IK-KK----------------------------------------------------------------------------------------------------------------------------------------RKET--RFIDESERVMDVIAFWENEQYLFGSQLNSSFIHSNSKQNDEGI--EQF---------------------------NLQ-N-----Y-QDL-------E---GLKI-----------------------YLGIRILFSFQENDKE-------------------------AISVFY-T--Q---------LSSEVLEGKY----------------APL---EEKDYLNLAALQLCIDFPRDVILKEISKRQSINADS---------------------GS---------------------PQRTQSNFLSATSQSESGASQLKKLSYQD--------------IEQIDE---QDEYE-QFI---------------QNRNEILIDPV--FYI---PAKMY-FQK-------D---IQQWQHLILQNLVDNRLLKKEYTP-------SQAK-LAYILYLSKQDLFMST-------------------IF 
 
(a)Residue pairs that are structurally aligned by TM-align program are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade.(more about the colors used)
(b)Ranking of proteins is based on TM-score of the structural alignment between Model1 and the PDB structures in our template library.
(c)RMSDa is the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.

  Function Prediction

  Predicted EC Numbers

RankTM-scoreRMSDaIDENaCov.EC-ScorePDB
Hit		EC No.
10.6550 4.41 0.13 0.860.75642j0mA 2.7.10.2
 
20.6676 3.74 0.12 0.830.74272j0jA 2.7.10.2
 
30.3905 6.77 0.07 0.690.39121aorB 1.2.7.5
 
40.3886 6.70 0.07 0.680.38713pflA 2.3.1.54
 
50.3863 6.51 0.07 0.660.38173chsA 3.3.2.6
 
(a)Ranking is based on EC-score, which is a confidence score for the Enzyme Classification (EC) Number prediction.
(b)RMSDa is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)IDENa is percentage sequence identity in the structurally aligned region.
(d)Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)EC-Score is defined based on the C-score of the structure prediction and similarity of the model with known enzyme structures, as identified using both global and local structural alignment programs. The global similarity score uses TM-score, IDENa,RMSDa and Cov. of the structural alignment by TM-align, while the local match compares the structural and chemical similarity of local spatial motifs in the model with known catalytic site of enzymes. A prediction with a EC-score >1.1 signifies a prediction with high confidence (upto 3 digit numbers of EC) and vice versa (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).

  Predicted GO terms

RankTMscoreRMSDaIDENaCov.PDB
Hit		Fh-ScoreAssociated GO Terms
10.7362 2.69 0.12 0.831ni2B 0.81 GO:0043621 GO:0050839 GO:0051015 GO:0007155 GO:0009653 GO:0010926 GO:0016043 GO:0022406 GO:0022610 GO:0030029 GO:0033036 GO:0044085 GO:0048856 GO:0048869 GO:0050793 GO:0050794 GO:0051235 GO:0065008 GO:0001726 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005884 GO:0005886 GO:0005902 GO:0005938 GO:0015629 GO:0016324 GO:0019898 GO:0030175 GO:0030863 GO:0031252 GO:0043229 GO:0044448 GO:0045177
20.7389 2.87 0.11 0.831isnA 0.77 GO:0008092 GO:0007049 GO:0007165 GO:0008283 GO:0009790 GO:0010648 GO:0010926 GO:0016043 GO:0044092 GO:0048519 GO:0048646 GO:0048856 GO:0051726 GO:0001726 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005856 GO:0005886 GO:0005912 GO:0019898 GO:0030027 GO:0030175 GO:0031252 GO:0032153 GO:0032154 GO:0043229
30.7296 2.87 0.10 0.832i1kA 0.74 GO:0008092 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0016020 GO:0019898 GO:0043229
40.7338 2.94 0.10 0.832yvcB 0.73 GO:0003779 GO:0010926 GO:0019222 GO:0022607 GO:0030029 GO:0030030 GO:0033036 GO:0043283 GO:0043933 GO:0044087 GO:0044237 GO:0044238 GO:0048519 GO:0050794 GO:0060255 GO:0065008 GO:0001726 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005886 GO:0005902 GO:0019898 GO:0030027 GO:0030175 GO:0031252 GO:0032420 GO:0032421 GO:0043229 GO:0045177
50.7198 2.84 0.10 0.821sghA 0.72 GO:0005102 GO:0005200 GO:0008092 GO:0050839 GO:0002376 GO:0006928 GO:0007159 GO:0022610 GO:0022614 GO:0050900 GO:0001931 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005886 GO:0005902 GO:0016323 GO:0016324 GO:0019898 GO:0030175 GO:0043229 GO:0045177
60.6989 3.07 0.10 0.813binA 0.70 GO:0003779 GO:0005198 GO:0030029 GO:0030036 GO:0030866 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005886 GO:0005911 GO:0019898 GO:0043229
70.6843 3.13 0.08 0.791gg3C 0.65 GO:0003779 GO:0005200 GO:0005545 GO:0008015 GO:0030029 GO:0030036 GO:0030866 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0005938 GO:0008091 GO:0015629 GO:0019898 GO:0030863 GO:0030864 GO:0043229 GO:0043234 GO:0044448
80.4358 6.37 0.10 0.732jqxA 0.50 GO:0000287 GO:0004474 GO:0006081 GO:0006084 GO:0006097 GO:0006099 GO:0009056 GO:0009987 GO:0044248 GO:0046356 GO:0046487 GO:0005622 GO:0005623 GO:0005737
90.3905 6.77 0.07 0.691aorB 0.39 GO:0005506 GO:0009055 GO:0033726 GO:0043169 GO:0051539 GO:0055114
100.3867 6.31 0.07 0.642jqxA 0.39 GO:0000287 GO:0004474 GO:0006081 GO:0006084 GO:0006097 GO:0006099 GO:0009056 GO:0009987 GO:0044248 GO:0046356 GO:0046487 GO:0005622 GO:0005623 GO:0005737

Consensus Prediction of Gene Ontology terms 
Molecular Function  Biological Process Cellular Location
GO termGO-Score  GO termGO-Score GO termGO-Score
GO:00080920.511  GO:00099870.565  GO:00057370.639
GO:00037790.289  GO:00160430.365  GO:00056230.639
GO:00051980.206  GO:00300290.289  GO:00056220.639
GO:00508390.152  GO:00081520.241  GO:00160200.511
GO:00052000.136  GO:00488560.238  GO:00198980.511
GO:00038240.128  GO:00507890.231  GO:00058560.511
GO:00431670.128  GO:00507940.231  GO:00432290.511
GO:00468720.128  GO:00109260.231  GO:00058860.437
GO:00167460.089  GO:00650070.231  GO:00301750.302
GO:00167400.089  GO:00511790.226  GO:00429950.302

(a)Ranking in the first table is based on a function prediction score (Fh-score), which is calculated based on the C-score of the structure prediction and the TM-score, IDENa, RMSDa and Cov. of the structural alignment by TM-align between the predicted model and the PDB structures (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).
(b)RMSDa is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)IDENa is the percentage sequence identity in the structurally aligned region.
(d)Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)A consensus prediction of GO terms is derived from the structural analogs that have an Fh-score of >=1.0. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on the Fh-score of the template from which the GO term is derived. A prediction with a GO-score >0.5 signifies a prediction with high confidence and vice versa.


  Predicted Binding Site


Binding site residues in the model:
GLU:51  VAL:52  ARG:53  ASN:54  ASN:55  ILE:56  LYS:57  
LYS:59  ARG:60  LYS:61  GLU:62  THR:63  ARG:64  VAL:75  
ILE:76  ALA:77  PHE:78  TRP:79  GLU:80  ASN:81  GLU:82  
GLN:83  TYR:84  LEU:90  PHE:94  ILE:95  HIS:96  ASN:98  
ASP:103  ILE:106  PHE:109  ASN:110  LEU:111  GLN:112  ASN:113  
TYR:114  
Identified analogs with similar binding site:

RankPDB
Hit		TM-scoreRMSDaIDENaCov.BS-scoreDownload
Complex		Binding site residues on the predicted model
13b4wA0.3659 6.68 0.07 0.65 0.65Download51,52,53,54,55,56,57,59,60,61,62,63,64,75,76,77,78,79,80,81,82,83,84,90,94,95,96,98,103,106,109,110,111,112,113,114
21ky8A0.3597 6.60 0.07 0.62 0.56Download51,52,53,54,55,56,60,61,62,63,64,93,94,95,96,97,98,99,101,103,104,105,106,107,108,109,110,111,112,113,114,121,123,287,288,289,290,291,292,293,294
31qi1A0.3640 6.58 0.07 0.63 0.29Download50,51,52,53,54,55,61,62,63,64,65,76,80,83,90,92,94,95,96,97,98,99,101,105,106,107,108,109,110,111,112,113,114,115,120,121,122,123,292,296
41jnrA0.4102 5.87 0.08 0.64 0.27Download9,10,11,12,13,14,15,49,50,51,52,53,63,64,65,66,121,122,123,124,125,145,148,149,150,152,153
51aorA0.3873 6.75 0.06 0.69 0.24Download61,147,148,149,150,151,152,153,154,155,156,157,158,238,239
61qi1A0.3640 6.58 0.07 0.63 0.18Download65,126,127,128,129,295,296,297,299,300,303
72e1qA0.3921 6.35 0.06 0.66 0.13Download2,3,4,5,6,7,8,9,17,18,19,20,21,29,30,31,33,34,35,37,38,117,118,124
82bhpA0.3610 6.82 0.07 0.65 0.03Download10,11,12,47,48,49,50,52,65,109,112,115,121,123,124,125,126,127,146

(a)Ranking of the analogs in the table is based on their BS-score. BS-score is calculated by first finding the structural analogs of the predicted model based on their TM-score, IDENa and Cov. of the structural alignment. The ligand(s) in the analog structure are then transferred onto the model and the fitness of the ligand-model complex (BS-score) is calculated by comparing the local structure and sequence similarity in the binding site region. (For detail, see Kucukural, Szilagyi, Ambrish, and Zhang, Template based ligand binding site prediction on modeled protein structure, 2009, in preparation).
(b) A BS-score of >0.5 signifies a binding site prediction with high confidence and vice-versa.
(c)RMSDa the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.
(f)The image shows the ligand-protein complex with the best BS-score. The ligand is depicted in magenta colored ball & stick, the predicted binding site residues interacting with the ligand are shown as transparent green spheres, while the N & C terminus in the model are marked by blue and red spheres respectively.



 Please cite following articles when you use the I-TASSER server:
1.Yang Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9:40 (2008).
2.Yang Zhang. Template-based modeling and free modeling by I-TASSER in CASP7. Proteins, 8: 108-117 (2007).
3.Sitao Wu, Jeffrey Skolnick, Yang Zhang. Ab initio modeling of small proteins by iterative TASSER simulations. BMC Biology, 5:17 (2007).