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I-TASSER results for job id S34208

Submitted Sequence

>B41
NKKLINQIAIICQNGYLKIKIPKEKEINIKNEEAYQAKNVGIRNLCIQQIKYYRNPNKTK
PVVQNQTSTIQQNKQQNQQPQKSQNQVTSSFQVNNQMMQQKQSLVGQQIQSIVNELNLGE
IQINKTQQDLKDFRKSKIIPQVNLNPSHSKRASNAWRQTLIASSSQNKINKDLQLSELGE
QKLINMKETTDQKEKIKYTKPLILQFTEYNPAYQYAEFNMESAIDLEDVPNSLDFEGELN
DLISEEIIQQIKKNNFYSHLSFILAKRTKWGITQTQEKIMRHQLKPISNPLTNIADLKNI
ITYEDTKDAVQSIRSIIQQNNLYNDTSTQTQRPINYEDARTGKLDKQVSKQITNDQSQKL
ATVFSKYSLMNPRKASVRDTNSLEQIVLQQRKLTKLIQYKKFSPSEKAINIFKTLLKCTG
ERKSRYNRHNNIIKLLTLCNEESIDLKEEVYVQLCKQLSANDKKESRIKYLKLFAIISSI
LPTTSRFYYPLLHFLYMRNTDTQIEQDERKCEQIQRGCIPTDTEIVMIENKKQILIRVYL
LNEIPITVGVESYTSVKMLKQELLQKIGITQRHEYYGLMEVRNNIKKKRKETRFIDESER
VMDVIAFWENEQYLFGSQLNSSFIHSNSKQNDEGIEQFNLQNYQDLEGLKIYLGIRILFS
FQENDKEAISVFYTQLSSEVLEGKYAPLEEKDYLNLAALQLCIDFPRDVILKEISKRQSI
NADSGSPQRTQSNFLSATSQSESGASQLKKLSYQDIEQIDEQDEYEQFIQNRNEILIDPV
FYIPAKMYFQKDIQQWQHLILQNLVDNRLLKKEYTPSQAKLAYILYLSKQDLFMSTIFRD
VKYQRIKIKTLNDTLSSKEKYEQMIQQNQQIPIKDEILLAINPSNILMCDSIGLNRPKRR
VQIKNIQKFCCTEKEVIVISHDNTKHIFETPQYCREIAFLIRSYLRIFYQKQQQQQQQEK
SQ

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 620 640 660 680 700 720 740 760 780 800 820 840 860 880 900 920 940 960 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| NKKLINQIAIICQNGYLKIKIPKEKEINIKNEEAYQAKNVGIRNLCIQQIKYYRNPNKTKPVVQNQTSTIQQNKQQNQQPQKSQNQVTSSFQVNNQMMQQKQSLVGQQIQSIVNELNLGEIQINKTQQDLKDFRKSKIIPQVNLNPSHSKRASNAWRQTLIASSSQNKINKDLQLSELGEQKLINMKETTDQKEKIKYTKPLILQFTEYNPAYQYAEFNMESAIDLEDVPNSLDFEGELNDLISEEIIQQIKKNNFYSHLSFILAKRTKWGITQTQEKIMRHQLKPISNPLTNIADLKNIITYEDTKDAVQSIRSIIQQNNLYNDTSTQTQRPINYEDARTGKLDKQVSKQITNDQSQKLATVFSKYSLMNPRKASVRDTNSLEQIVLQQRKLTKLIQYKKFSPSEKAINIFKTLLKCTGERKSRYNRHNNIIKLLTLCNEESIDLKEEVYVQLCKQLSANDKKESRIKYLKLFAIISSILPTTSRFYYPLLHFLYMRNTDTQIEQDERKCEQIQRGCIPTDTEIVMIENKKQILIRVYLLNEIPITVGVESYTSVKMLKQELLQKIGITQRHEYYGLMEVRNNIKKKRKETRFIDESERVMDVIAFWENEQYLFGSQLNSSFIHSNSKQNDEGIEQFNLQNYQDLEGLKIYLGIRILFSFQENDKEAISVFYTQLSSEVLEGKYAPLEEKDYLNLAALQLCIDFPRDVILKEISKRQSINADSGSPQRTQSNFLSATSQSESGASQLKKLSYQDIEQIDEQDEYEQFIQNRNEILIDPVFYIPAKMYFQKDIQQWQHLILQNLVDNRLLKKEYTPSQAKLAYILYLSKQDLFMSTIFRDVKYQRIKIKTLNDTLSSKEKYEQMIQQNQQIPIKDEILLAINPSNILMCDSIGLNRPKRRVQIKNIQKFCCTEKEVIVISHDNTKHIFETPQYCREIAFLIRSYLRIFYQKQQQQQQQEKSQ
Prediction	CCCHHHHHHHHCCCCSSSSSCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCHHHHSSCHHHHHHHHHHHHHHHHHHHHHCCCCCSSSCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCCSSSSCCCCCHHHHHHCCHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHCSSSCCCCCCCCCCCCCSSSSSSSCCCCCCCCCCCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHCCCCCCCCCSSSCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCSSSSSSSCCCCSSSSSSCCCCHHHHHHHHHHHHCCCCCCCCCSSSSSSSCCCCCCCCCSSSCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCSSSSSSSCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCHCCCCCCCCHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCSSSCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCSSSSSSCCCCCCCHHHHHHHHHHHCCCCCCCCCSSSSSCHHHSSSSCCCCCCCCHHHHHHHHSSSSCCCCCCSSSSSSCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
Conf.Score	90055456631017747873573000465558776421422888999988872487768774005312777656523485022200012421308999988878889999998651432034131277788877545565414683000234668898987502200001111155552666503200233465421455305752035707766530123116553387644554136677689999998753438778999864420351002767744666699999976666510232256777657757532322321576533447625654655654033111031478888899865676404367663005566655321221254010158578999999999999982887789982079999999865168336779999999985089987799999999999983579937789999998502699726689899997137889998999999967984579999739978999933745299999999998099988884699999788666565113415874206988899999986412312323322344565310120232001255530378988617888996588999999988999879846999999999999999987186426778732020047888838888788866344331452225778888743151337899876067345460442124222065677899999876555167661369999999999986324344331311310024454304531001577899887502589874147872567724310355568313322322214248998659998447266402728899999999999999999999887765039

Top 5 Models predicted by I-TASSER


Download Model 1	Download Model 2	Download Model 3	Download Model 4	Download Model 5
C-score=-0.92	C-score=-1.57	C-score=-1.16	C-score=-2.49	C-score=-2.60

Estimated accuracy of Model1: 0.60±0.14 (TM-score) 11.0±4.6Å (RMSD) (Read more about C-score of generated models)

Top 10 templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov.

Norm.
Z-score

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560                 580                 600                 620                 640                 660                 680                 700                 720                 740                 760                 780                 800                 820                 840                 860                 880                 900                 920                 940                 960
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Sec.Str
Seq

CCCHHHHHHHHCCCCSSSSSCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCHHHHSSCHHHHHHHHHHHHHHHHHHHHHCCCCCSSSCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCCSSSSCCCCCHHHHHHCCHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHCSSSCCCCCCCCCCCCCSSSSSSSCCCCCCCCCCCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHCCCCCCCCCSSSCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCSSSSSSSCCCCSSSSSSCCCCHHHHHHHHHHHHCCCCCCCCCSSSSSSSCCCCCCCCCSSSCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCSSSSSSSCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCHCCCCCCCCHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCSSSCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCSSSSSSCCCCCCCHHHHHHHHHHHCCCCCCCCCSSSSSCHHHSSSSCCCCCCCCHHHHHHHHSSSSCCCCCCSSSSSSCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NKKLINQIAIICQNGYLKIKIPKEKEINIKNEEAYQAKNVGIRNLCIQQIKYYRNPNKTKPVVQNQTSTIQQNKQQNQQPQKSQNQVTSSFQVNNQMMQQKQSLVGQQIQSIVNELNLGEIQINKTQQDLKDFRKSKIIPQVNLNPSHSKRASNAWRQTLIASSSQNKINKDLQLSELGEQKLINMKETTDQKEKIKYTKPLILQFTEYNPAYQYAEFNMESAIDLEDVPNSLDFEGELNDLISEEIIQQIKKNNFYSHLSFILAKRTKWGITQTQEKIMRHQLKPISNPLTNIADLKNIITYEDTKDAVQSIRSIIQQNNLYNDTSTQTQRPINYEDARTGKLDKQVSKQITNDQSQKLATVFSKYSLMNPRKASVRDTNSLEQIVLQQRKLTKLIQYKKFSPSEKAINIFKTLLKCTGERKSRYNRHNNIIKLLTLCNEESIDLKEEVYVQLCKQLSANDKKESRIKYLKLFAIISSILPTTSRFYYPLLHFLYMRNTDTQIEQDERKCEQIQRGCIPTDTEIVMIENKKQILIRVYLLNEIPITVGVESYTSVKMLKQELLQKIGITQRHEYYGLMEVRNNIKKKRKETRFIDESERVMDVIAFWENEQYLFGSQLNSSFIHSNSKQNDEGIEQFNLQNYQDLEGLKIYLGIRILFSFQENDKEAISVFYTQLSSEVLEGKYAPLEEKDYLNLAALQLCIDFPRDVILKEISKRQSINADSGSPQRTQSNFLSATSQSESGASQLKKLSYQDIEQIDEQDEYEQFIQNRNEILIDPVFYIPAKMYFQKDIQQWQHLILQNLVDNRLLKKEYTPSQAKLAYILYLSKQDLFMSTIFRDVKYQRIKIKTLNDTLSSKEKYEQMIQQNQQIPIKDEILLAINPSNILMCDSIGLNRPKRRVQIKNIQKFCCTEKEVIVISHDNTKHIFETPQYCREIAFLIRSYLRIFYQKQQQQQQQEKSQ

2bkuB

0.10

0.16

0.84

1.25

Download

STEFAQLLENSILS-------PDQNIRLTSETQLKKLSNDNFLQFAGLSSQVLIDENTKNELVSKDSVKTQQFAQRWITSPEAKNQIKTNA---LTALVSIEPRIANAAAQLIAAIADIELPHGAWPELMKIMVD-------NTGAEQPENVKRASLLALGYMCES--ADALVSSSNNILIAIVQGAQSTETSKAV---------------RLAALNALADSLIFIKNNMEREGERNYLMQVVCEATQIEVQA-AAFGCLCKIMSKYYTF-MKPYMEQALYALT---------IATMKS--------------------------------------------PNDKVASMTVEFWSTICEEEIDIAYELAQFPQSPLQSYNFALSSIKDPNLLNLLTRQNEDVSMSAGACLQLFAQNCGN-----HILEPVLEFVEQNITANWRNREAAVMAFGSIMDGPDKVQRTYYVHQALPSILNLMDQSETTAWCIGRIADSIDPQQHLPGVVQACLIGLQDHPKVATNCSWTIIN--------LVEQLAEATPSPIYNFYPALVDGLIGAANRIDNEFNASAFSALTTMVEYATDT--------VAETSASISTFVMDKLGQTMSVDENQLTLEDAQSLQELQSNILTVLA--------AVIRKSPSSVEPVADMLMGLFFRLLEKKDSAFIEDDVFYAISALAASLGKGFEKYLETFSPYKALNQVDSPVSITAVGFIADISNSEDFRRYSDAMMNVLAQMISNPNARRELKPA----VLSVFGDIASNIGA--DFIPYLNDIMALCVAAQNTKPE-NGTLEALDYQIKVLELDAY-VGIVAGLHD---KPEALFPYVGTIFQFIAQVAEDPQLYSEDATSVGLIGDIAAMF---PDGSIKQFYGQDWVIDY---------I----KRTR--SGQLFSQATKDTARWAREQQKRQLSL-------

1h4rA

0.12

0.05

0.29

5.14

Download

-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FTVRIVTMDAEM-EFNCEMKWKGKDLFDLVCRTLGLRE-TWFFGLQYTI------KDTVAWLKMDKKVLDHD-------------------------------------VSKEEPVTFHFLAKFYPENELVQEITQHLFFLQVKKQILDEKI-YCPPEASVLLASYAVQAKYGDYDPSV---------------H---------------------K-----------------------RGFLAQEELLPKRVINQMTPEMWEER----ITAWYAEHRGRARDEAEMEYLKIAQDLEMYGVNYFAIRN-------------------------KK-G---TELLLGVDALGLHIYDPENRLTPKISFPWNEIRNISYS-DKEFTIKPLDKVFKFNSSKVNKLILQLCIGNHDLFMRRRKA--------

1qgrA

0.08

0.16

0.84

2.76

Download

NPGNSQVARVAAGLQIKNSLTSKDPDIKAQYQQRWLAIDANARREVKNYVLHTLGTETYRPSSASQCVAGIACAEIPVNQWPELIPQLVANVTNPNSTEHMKESTLEAIGYICQDIDPEQLQDKSNEILTAIIQGMRKE----EPSNNVKLAATNALLNSLEFTKANFDKESERHFIMQVVCEATQCPDTRVRVAAL-------------QNLVKIMSLYYQYMETYMGPALFAITIEAMKSDIDEVALQGIEFWSNVCDEEMDLAIE------ASEAAEQGRPPEHTSKFYAKGALQYLVPI----------------------LTQTLTKQDENDDDDDWNPCKAAGVCLMLLATCCEDDIVPHVL----------------------PFIKEHIKNPDWRYRDAAVMAFGCILEGPEPSQLKPLVIQAMPTLIELMKDPSVVVRDTAICELLPEAAINDVYLA-----PLLQCLIEGLSAEPRVASNVCWAF---------SSLAEAAYEAADVA-----------------------DDQEEPATYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYE-----SLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNV-----------LRKVQHQDALQISDVVMASLLRMFSGGVQEDALMAVSTLVEVLGGEFLKYMEAFKPFLGIGLKNYAEYQVCLAAVGLVGDLCRALQSNIIPFCDEVM-----QLLLENLGNENVHRSVKPQILSVFGDIALAIGGEFKKYLEVVLNTLQQASQAQVDKSDYDMVDYLNEL-----------------------RESCLEAYTGIVQGLKGDQENVHPDVMLVQPRVFIDHIAGDEDHTDGVVACAAGLIG----DLCTAFGKDVLKLVEARPMIHELLTEGRKAKTLARWATKELRKLKNQA

1h4rA

0.13

0.05

0.30

2.05

Download

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KTFTVRIVTMDA-EMEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTI-------KDTVAWLKMDKKVLD-------------------------------------HDVSKEEPVTFHFLAKFYPENAEVQEITQHLFFLQVKKQILDEKI-YCPPEASVLLASYAVQAKYGDYDP--SVHKRGFLAQEELLPKRVI-------NLYQMTPEMWEERITAWYAEHR----------------------------------------------------GRARDEAEMEYLKIAQDLEMYGVNYF---AIRN-----------------------KKG---TELLLGVDALGLHIYDPENRLTPKISFPWNEIRNIS-YSDKEFTIKPLDKKFKFNSSRVNKLILQLCIGNHDLFMRRRKA--------

1qgrA

0.12

0.16

0.85

1.23

Download

----MELITIL------------EKTVSPDRLELEAAQKF-LERAAVENLPTF-------------LVELSRVLANPGNSQVARVAAGLQI---KNSLTSKDPDIKAQYQQRWLAID----------ANARREVKNYVLHTLGTETYRPSSASQCVAGIACAEIPVNQWP---ELIPQLVANVTNPNSTEHMKEST---------------LEAIGYI-CQD-ID----PEQLQ---DKSNEILTAIIQGMRKEEPSNNVKLAATNALLNSLEFTKANFDKESERHF------MQVVCEATQCPDTRVRVAALQNLVK-SLYYQYMETYMGPAIEAMKSDIDEVALQGIEFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKFYAKGALQYPILTQTLTKQDENPCKAAGVCLMLLATCCEDDKNPDWRYRDAAVMAFGCILEGKPLVIQAMPTLIELM-KDPSVVVRDTAAWTVGRICELLPEAAPLLQCLIEGLSAE-----PRVASNVCWAF-------SSLAEAAYEAADV------ADDQEEPATYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYESL-----MEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQN--------VLRKVQHQDALQISDVVMASLLRMFSGG---VQEDALMAVSTLVEVLGGEFLKYMEAFKPFLGIGLKNYAEYQVCLAAVGLVGDLCRAIPFCDEVMQLLLENLGNENVHRSVKPQ----ILSVFGDIALAI---GEFKKYLEVVLNTLQQASQAQVD-KSDYDMVDYLNELRELEAY-TGIVQGLKGDQENVHPDVMLVQPVEFILSFIDHIAGDEDHTDGVVACAAGLI-LCTAFGKDVLKLVEARPMIHEL--------------TEGRRSKTNKAKTLARWATKELRKLKNQA----------

1h4rA

0.12

0.05

0.30

4.42

Download

-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FTVRIVTMDAE-MEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKD-------TVAWLKMDKKVLDHD-------------------------------------VSKEEPVTFHFLAKFYPENELVQEITQHLFFLQVKKQILDEKI-YCPPEASVLLASYAVQAKYGDYDPSVHKR--GFLAQEELLPKRVIN--------------------------L-----------------------------YQMTPEMWEERIT----AWYAEHRGRARDEAEMEYLKIAQDLEMYGVNYFAIRNKKG-----------------------------TELLLGVDALGLHIYDPENRLTPKISFPWNEIRN-ISYSDKEFTIKPLDKKFKFNSSRVNKLILQLCIGNHDLFMRRRKA--------

1qbkB

0.08

0.17

0.83

2.61

Download

QSPDTTIQRTVQQKLEQLNQYPDFNNYLIFVLTKLKSEDEPTRSILKNNVKAHFQNFPNGVTDFIKSECLNNIGDSSPLIRATVGILITTIASKGELQNWPDLLPKLCSLLDSEDYNTCEGAFGALQKICEDSAEILDSDRPLNII---------PKFLQFFKHSSPKIRSHAVACVNQFIISRTQALLHIDSFTENLFALAGDEEPEVRKNVCRALVLLEVRMDRLLPHHNIVEYLQRT------------QDQDENVALEACEFWLTLAEQPICKDVLVRHLPKLIPVLVNGKYSDIDIILLKGDVEEDETIPDSEQDIRPRFHRSRTVAQQHDEDDDDDDEIDDDDTISDWNLRKCSAAAL-------------DVLANVYRDELLPHILPLLKELLFHHEWVVKESGILVLGAIAEGCQGIPYLPELIPHLIQCLSDKKALVRSWTLSRYAHWVVSQPPDTYLKPLTELLKRILDSNKRVQEAACSAFATLEEEACTELVPYLAYILDTLVFAFSKYQHKNLLIL-----------------------YDAIGTLADSVGHHLNKPEYIQLPPLIQKWNLKDEDKD----------LFPLLECLSSVATA-------------------------------------------LQSGFLPYCEPVYQRCVNLVQKTLDQYEAPDKDFIVALDLLSGLAEGLGGNIEQLVARSNILTLYQCQDKPEVRQSSFALLGDLTKACFQHVKPCIADFPILGTNLNPEFI----------------------SVCNNATWAIGEISIQGEIINRPNTPKTLLENTAITIGRLGYVCPQEVAPLQ----------------QFIRPWCTSLRNIRDNEEK-----DSAFRGICTISVNPSGVIQDFIFFCDAVASWIN--------------PKDDLRDFCKILHGFKNQVGDENWRRFSDAFYG

2al6A

0.14

0.08

0.34

1.25

Download

-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAMERVLKVFHYFENSSEPTIRHGDATDVRGIIQKIVDCHKVKNVACYGLRLSHLQS-----EEVHWLHLDMGVSNVREKFELAH--------------------------------PPEEWKYELRIRYLLNQFTEDKPTLNFFYQQVKNDYMLEIADQVDQEIALKLGCLEIRRSYGE---MRGNALEKKSNYEVLEKDVGLRRFFPKSLLDSVKAKTLRKLIQQTFRQLNREESILKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGISYLTDKGANPTHLADFNQVQTIQYSNSGMLQLKIAGAPEPLTVTA--PSLTIAENMADLIDGYCRLVNGATQSFIIRPQTDDYAEIIDE--------------------------------------------------------------------

2fgeA

0.11

0.18

0.90

1.19

Download

-----DEAEKL---GFEKVSEEFISECKSKAIL-FKHKKTGCE--------VSVSNEDENKVFG-------------RTPPKDSTGIPHILQ--HSVLCGSRKYPVKEPFVELLKGSLHTFLNAFTYPDR-------CYPVASTNTKDFYNLVDVYLDAVFFP----KCVDD---AHTFQQEGW-HYELNDPSEDISYKGVVFNEKGVYSQPDNILGRIAQQAL-SPENTYGVDSGGDIPNLTFEEFKEFHRQYDDPVHRLRVLSEYLDFEASPSPNSSKIKFQKLFSEP-------VRLVEKYPAGRDGDLKKKHLCVNWLLSEKPLDLQT----------QLALGFLDHLLGTPASPLRKILLESGLGEALVSSGLS-DELLQPQFG------GLKGVSEENVQKVEELIDTLKKLAEEGFDNDAVEASNTIEFSLRENNTGPRGLSLLQSISKWIYDDPKYTEPLKALKTRIAEEG---SKAVFSPLIEKLILNNSPEKATQEEVEEKNILEKVKAATEEDLAELARATPDPPEAL---RCVPSLNLDIPKEPTYVPTEVGDINGVKFTNDIIYTEVVFDIGSLKHELLPLVPLFCQSLLEFVQLNQLIGRKTGGISVYPLTSSVRGKDEPCSKIIVRGKSAGRADDLFNLNCLLQEVQFTDQQRFKQFVSQSRARENR---LRGSGHGIAAARDALNIAGWSEQGGLSYLEFLHTLKKVDEDWEGISSSLEEIRRSLGKSLTNVEKSVAKFLDLLPENVTWDGRLPLRNEAIVIPTQGKAGNIYSTGYELDGSAYVISKHISNTWLWDRVRVSG--GAY--------GGFCDFDSHSGVFSYLSYRDPNLLKTLDIYDGTGDFLRGLDVDQETLTKAIIGTIGDVDSYQLPDAKGYSSLLRHL-LGVTDEERQRK----REEILTTS--LKDFKDFAQAIDVVRDKSAEDIDAANNER

1h4rA

0.12

0.05

0.29

4.36

Download

-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FTVRIVTMDAE-MEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKDTVAW-------LKMDKKVLD-------------------------------------HDVSKEEPVTFHFLAKFYPENELVQEITQHLFFLQVKKQILDEKI-YCPPEASVLLASYAVQAKYGDYDPQEELLPKRVINLYQMTPEMWEER----------------------------------------------------------------------ITAWYAEHRGRARDEAEMEYLKIAQDLEMYGVNYFAIRNKKG-----------------------------TELLLGVDALGLHIYDPENRLTPKISFPWNEIRN-ISYSDKEFTIKPLDKKFKFNSSRVNKLILQLCIGNHDLFMRRRKA--------

(a)	All the residues are colored in black; however, those residues in template which are identical to the residue in the query sequence are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade. (more about the colors used)
(b)	Rank of templates represents the top ten threading templates used by I-TASSER.
(c)	Ident1 is the percentage sequence identity of the templates in the threading aligned region with the query sequence.
(d)	Ident2 is the percentage sequence identity of the whole template chains with query sequence.
(e)	Cov. represents the coverage of the threading alignment and is equal to the number of aligned residues divided by the length of query protein.
(f)	Norm. Z-score is the normalized Z-score of the threading alignments. Alignment with a Normalized Z-score >1 mean a good alignment and vice versa.
(g)	Download Align. provides the 3D structure of the aligned regions of the threading templates.
(h)	The top 10 alignments reported above (in order of their ranking) are from the following threading programs:
	1: MUSTER 2: HHSEARCH 3: PROSPECT2 4: SP3 5: MUSTER 6: HHSEARCH I 7: PROSPECT2 8: FUGUE 9: MUSTER 10: HHSEARCH II

10 proteins in PDB which are structurally closest to the first I-TASSER model (identified by TM-align)

Rank	TM-score	RMSD^a	IDEN^a	Cov.	PDB Hit
1	0.8349	0.98	0.08	0.84	1qgrA Model1

2	0.6412	4.35	0.07	0.73	2bkuB Model1

3	0.5944	6.26	0.08	0.77	1wa5C Model1

4	0.4876	6.51	0.07	0.63	1qbkB Model1

5	0.4356	7.34	0.06	0.60	1u6gC Model1

6	0.3854	7.47	0.05	0.53	3ebgA Model1

7	0.3803	8.88	0.04	0.60	1llwA Model1

8	0.3735	9.05	0.03	0.59	1ea0A Model1

9	0.3719	4.80	0.07	0.44	1gw5A Model1

10	0.3620	5.18	0.05	0.43	1b3uA Model1

Structural alignment using TM-align
MELITILEKTVSPDRLELEAAQKFLERAAVENLPTFLVELSRVLANPGNSQVARVAAGLQIKNSLTSKDPDIKAQYQQRWLAIDANARREVKNYVLHTLGTETYRPSSASQCVAGIACAEIPVNQWPELIPQLVANVTNPNSTEHMKESTLEAIGYICQDIDPEQLQDKSNEILTAIIQGMRK--E--EPSNNVKLAATNALLNSLEFTKANFDKESERHFIMQVVCEATQCPDTRVRVAALQ-------------NLVKIMSLYYQYMETYMGPALFAITIEAMKSDIDEVALQGIEFWSNVCDEEMDLAIE------ASEAAEQGRPPEHTSKFYAKGALQYLVPI----------------------LTQTLTKQDENDDDDDWNPCKAAGVCLMLLATCCEDDIVPHVL----------------------PFIKEHIKNPDWRYRDAAVMAFGCILEGPEPSQLKPLVIQAMPTLIELMKDPSVVVRDTAAWTVGRICELLPEAAINDVYLA-----PLLQCLIEGLSAEPRVASNVCWAF---------SSLAEAAYEAADVA----------------------D-DQEEPATYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYESL-----MEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNVL-----------RKVQHQDALQISDVVMASLLRMFSGGVQEDALMAVSTLVEVLGGEFLKYMEAFKPFLGIGLKNYAEYQVCLAAVGLVGDLCRALQSNIIPFCDEVM-----QLLLENLGNENVHRSVKPQILSVFGDIALAIGGEFKKYLEVVLNTLQQASQAQVDKSDYDMVDYLNELR-----------------------ESCLEAYTGIVQGLKGDQENVHPDVMLVQPRVEFILSFIDHIAGDEDHTDGVVACAAGLIG----DLCTAFGKDVLKLVEARPMIHELLTEGRRSKTNKAKTLARWATKELRKLKNQA ---------------------------------------------NKKLINQIAIICQNGYLKIKIPKEKEINIKNEEAYQAKNVGIRNLCIQQIKYYRNPNKTKPVVQNQTSTIQQNKQQNQQPQKSQNQVTSSFQVNNQMMQQKQSLVGQQIQSIVNELNLGEIQINKTQQDLKDFRKSKIIPQVNLNPSHSKRASNAWRQTLIASSSQNKINKDLQLSELGEQKLINMKETTDQKEKIKYTKPLILQFTEYNPAYQYAEFNMESAIDLEDVPNSLDFEGELNDLISEEIIQQIKKNNFYSHLSFILAKRTKWGITQTQEKIMRHQLKPISNPLTNIADLKNIITYEDTKDAVQSIRSIIQQNNLYNDTSTQTQRPINYEDARTGKLDKQVSKQITNDQSQKLATVFSKYSLMNPRKASVRDTNSLEQIVLQQRKLTKLIQYKKFSPSEKAINIFKTLLKCTGERKSRYNRHNNIIKLLTLCNEESIDLKEEV-Y-V-QL---CKQLSANDKKESRIKYLKLFAIISSILPTTSRFYYPLLHFLYMRNTDTQIEQDERKCEQIQRGCIPTDTEIVMIENKKQILIRVYLLNEIPITVGVESYTSVKMLKQELLQKIGITQRHEYYGLMEVRNNIKKKRKETRFIDESERVMDVIAFWENEQYLFGSQLNSSFIHSNSKQNDEGIEQFNLQNYQDLEGLKIYLGIRILFSFQENDKEAISVFYTQLSSEVLEGKYAPLEEKDYLNLAALQLCIDFPRDVILKEISKRQSINADSGSPQRTQSNFLSATSQSESGASQLKKLSYQDIEQIDEQDEYEQFIQNRNEILIDPVFYIPAKMYFQKDIQQWQHLILQNLVDNRLLKKEYTPSQAKLAYILYLSKQDLFMSTIFRDVKYQRIKIKTLNDTLSSKEKYEQMIQQNQQIPIKDEILLAINPSNI--LM---CDSIGLNRPKRRVQIKNIQKFCCTEKEVIVISHDNTKHIFETPQYCREIAFLIR-S----YLRIFYQKQQQQQQQEKSQ

MSTAEFAQLLENSILSPDQNIRLTSETQLKKLSNDNFLQFAGLSSQVLIDENTKLEGRILAALTLKNELVSKDSVKTQQFAQRWITQVSPEAKNQIKTNALTALVSIEPRIANAAAQLIAAIADIELPHGAWPELMKIMVDNTGAEQPENVKRASLLALGYMCE-S----ADALVSSSNNILIAIVQGAQST-ETSKAVRLAALNALADSLIFIKNNMEREGERNYLMQVVCEATQAEDIEVQAAAFG-------------CLCKIMSKYYTFMKPYMEQALYALTIATMKSPNDKVASMTVEFWSTICEEEIDIAYE------LAQF----PQSPLQSYNFALSSIKDVVPN----------------------LLNLLTRQNEDPEDDDWNVSMSAGACLQLFAQNCGNHILEPVL----------------------EFVEQNITADNWRNREAAVMAFGSIMDGPDKVQRTYYVHQALPSILNLMNDQSLQVKETTAWCIGRIADSVAESIDPQQHLP-----GVVQACLIGLQDHPKVATNCSWTI---------INLVEQLAEA-T-P-------------------------------SPIYNFYPALVDGLIGAANRIDNEF-NARASAFSA-----LTTMVEYATDTVAETSASISTFVMDKLGQTMSVDENQLTLEDAQSLQELQSNILTVLAA-VI---------RKSPSSVEPVADMLMGLFFRLLEKKD--SAFIEDDVFYAISALAASLGKGFEKYLETFSPYLLKALNQV--------DSPVSITAVGFIADISNSLEEDFRRYSDAMMNVLAQMIS--N--PNA------------RRELKPAVLSVFGDIASNIGADFIPYLNDIMALCVAAQNTKPE--NG-----------------T-----------------------LEALDYQIKVLEAVLDAYVGIVAGLHDKPEALFPYVGTIFQFIAQVAEDP---------------------QLYSEDATSRAAVGLIGDIAAMFPDGSIKQFYGQDWVIDYIKRTR---------------------------------SGQLFSQAT--KDTARWAREQQKRQLSL- ----------------------------------------------NKKLINQIAIICQNGYLKIKIPKEKEINIKNEEAYQAK--NVG--IRNLCIQQIKYYRNPNKTKPVVQNQTSTIQQNKQQNQQPQKSQNQVTSSFQVNNQMMQQKQSLVGQQIQSIVNELNLGEIQINKTQQDLKDFRKSKIIPQVNLNPSHSKRASNAWRQTLIASSSQNKINKDLQLSELGEQKLINMKETTDQKEKIKYTKPLILQFTEYNPAYQYAEFNMESAIDLEDVPNSLDFEGELNDLISEEIIQQIKKNNFYSHLSFILAKRTKWGITQTQEKIMRHQLKPISNPLTNIADLKNIITYEDTKDAVQSIRSIIQQNNLYNDTSTQTQRPINYEDARTGKLDKQVSKQITNDQSQKLATVFSKYSLMNPRKASVRDTNSLEQIVLQQRKLTKLIQYKKFSPSEKAINIFKTLLKCTGERKSRYNRHNNIIKLLTLCNEESIDLKEEVY--V-QLC---KQLSANDKKESRIKYLKLFAIISSILPTTSRFYYPLLHFLYMRNTDTQIEQDERKCEQIQRGCIPTDTEIVMIENKKQILIRVYLLNEIPITVGVESYTSVKMLKQELLQKIGITQRHEYYGLMEVRNNIKKKRKETRFIDESERVMDVIAFWENEQYLFGSQLNSSFIHSNSKQNDEGIEQFNLQNYQDLEGLKIYLGIRILFSFQEND-KEAISVFYTQLSSEVLEGKYAPLEEKDYLNLAALQLCID--F--PR--DVILKEISKRQSINADSGSPQRTQSNFLSATSQSESGASQ-----L---KKLSYQDIEQIDEQDEYEQFIQNRNEILIDPVFYIPAKMYFQKDIQ-------------Q--WQHLILQNLVDNRLLKKEYTPSQAKLAYILYLSKQDLFMSTIFRDVKYQRIKIKTLNDTLSSKEKYEQMIQQNQQI-PI-K----------------DEILLAINPSNILMCDSIGLNRPKRRVQIKNIQKFCCTEKEVIVIS--------------------------------HDNTKHIFETPQYCREIAFLIRSYLRIFYQKQQQQQQQ----EKS----------------Q

MSDLETVAKFLAESVIASTAKTSERNL------------------RQLETQDGFGLTLLHVIASTNLPLSTRLAGALFFKNFIKRKWVDENGNHLLPANNVELIKKEIVPLMISLPNNLQVQIGEAISSIA--D---SDFPDRWPTLLSDLASRL-SNDDMVTNKGVLTVAHSIFKRWRPLFRSDELFLEIKLVLDVFTAPFLNLLKTVDEQITANEKASLNILFDVLLVLIKLYYDFNCQDIPEFFEDNIQVGMGIFHKYLSYSNPLLEHASVLIKVKSSIQELVQLYT------------TRYEDVFGPMINEFIQITWNLLTSISNQPKYDILVSKSLSFLT--A--------------------------VTRIPKYFEIFNNESAMNNIT--E------Q--IILPNVTLREEDVELFEDDPIEYIRRDL-EGTRRRACTDFLKELKEKN-E-----------V-----LV-TNIFLAHMKGFVDQYMSNWKFKDLYIYLFTALAINGNITNAGVSSTNNLLNVVDFFTKEIAPDLTSNNIPHIILRVDAIKYIYTFRNQLTKAQLIE-----LMPILATFLQTDEYVVYTYAAIT-I--------EKILTI--RESNTS-P-----------------------AFIFHKEDISNSTEILLKNLIALILKHGSSPEKLAENEFLMRS-----IFRVLQ-TSE--DSIQPLFPQLLAQFIEIVT-IM--A-----KN--PSNPRFTHYTFESIGAI--------LNYTQRQNLPLLVDSMMPTFLTVFSEDIQEFIPYVFQIIAFVVEQSATIPESIKPLAQPLLAPNVWE-LKGNIPAVTRLLKSFI-KTDSSIFPDLVPVLGIFQ--RLIA-S---------KA-YEVHGFDLLEHIMLLIDMNRLRPYIKQIAVLLLQRLQNSKT-----------------E-----------------------R-YVKKLTVFFGLISNKLGSDFLIHFIDEVQDGLFQQIWGNFIITTLP--------TIGN-LLDRKIALIGVLNMVINGQFFQSKYPTLISSTMNSIIETASSQSIANLKNDYVEEISTFGSHFSKLVSISEKPFDPLPEIDVNNGVRLYVAEALNKYNAISGNTFLNTILPQLTQENQVKLNQLLV ------NKKLINQIAIICQNGYLKI--KIPKEKEINIKNEEAYQAKNVGIRNLCIQQIKYYRN-----PNKTKP-VVQNQTSTIQQNKQQNQQPQ--K--SQNQVTSSFQVNNQMMQQKQSLVGQQIQSIVNELNLGEIQINKTQQDLKDFRKSKIIPQVNLNPSHSKRASNAWRQTLIASSS---Q---N------------------------------------------------KINKDLQLS-EL--GE-QKLINM-K---E--TTDQKEKIKYTKPLILQFTEYNPAYQYAEFNMESAIDLED-VP-NSLDFEG-ELN-DLISEEIIQQIKKNNFYSHLSFILAKRTKWGITQTQEKIMRHQLKPISNPLTNIADLKNIITYEDTKDAVQSIRSIIQQNNLYNDTSTQTQRPINYE--D--AR-TGK-LDKQVSKQITNDQSQKLATVFSKYSLMNPRKASVRDTNSLEQIVLQQRKLTKLIQYK--KFSPSEKAINIFKTLLKCT-------GERKSRYNRHNNII-KLLTLC-N--EESIDLKEEV-YV-QLCKQLSANDKKESRIKYLKLFAIISSILPTTSRFYYPLLHFLYMRNTDTQIEQDERKCEQIQRGCIPTDTEIVMIENKKQILIRVYLLNEIPITVGVESYTSVKMLKQELLQKIGITQ-R-HEYYGLMEVRNNIKKKRKETRFIDESERVMDVIAFWENEQYLFGSQLNSSFIHSNSKQNDEGIEQFNLQNYQDLEGLKIYLGIRILFSFQENDKEAISVFYTQLSSEVLEGKYAPLEEKDYLNLAALQLCID--FPRDVILKEISKRQSINADSGSPQRTQSNFLSATSQSESGASQLKKLSYQDIEQIDEQDEYEQFIQNRNEILIDPVFYIPAKMYFQKDIQ-------Q--WQHLILQNLVDNRLLKKEYTPSQAKLAYILYLSKQDLFMSTIFRDVKYQRIKIKTLNDTLSSKEKYEQMIQQNQQIPIKDEIL-LA-------I--NPSNILMCDSIGLNRPKRRVQIKNIQKFCCTEKEVIVIS-------------HDNTKHIFETPQYCREIAFLIRSY---------------------------LRIFYQKQQQQQQQEK--S-------Q--------------------

YEWKPDEQGLQQ-ILQLLKESQSPDTTIQRTVQQKLEQLNQYPDFNNYLIFVLTKLKSEDEPTR-------------------------------------------SLSGLILKNNV--K---------------AHFQNFPNGVTDFIKSECLNNIGDSSPLIRATVGILITTIAS----------------------KGELQNWPDLLPKLCSLLDSEDYNTCEGAFGALQKICEDSAEILDS-D--------------RPLNIIPKFLQFFKHSSPKIRSHAV-ACVNQFIISR-TQALL------------------HIDSFTENLFALAGDEEPEVRKNVCRALVLLE--V---------------------------------RMDRLLPH-HNIVEY-------------------LQRTQDQDE--------NVALEACEFWLTLAEQPICKDVLVRHL---------------------PKLIPVLVNG-KYSDIDIILLKGDVEEDETIPDSEQDIRPRFHRSRTVAQQHDEDDDDDDEIDDDDTISDWN-LRKCSAAALDVLANVYRD-E--LLPHILPLLKELLFHHEWVVKESGILVLGAIAEGCQGIP-YLPE-----LIPHLIQCLSDKKALVRSITCWTL---------SRYAHWVV--S--------------------------------QPP--DTYLKPLTELLKRILDSNK----RVQEAACSA-----FATLEEEACTELVPYLAYILDTLVFAF-SK--------------YQHKNLLILYDAIGTL-A---------DSVGHHLNKPEYIQLPPLIQKWNLKDEDKDLFPLLECLSSVATALQSGFLPYCEPVYQRCVNLVQKTLAQALNNAQPDQYEAPDKDFIVALDLLSGLAEGLGGNIEQLVARSNILTLY-QCQ--DK-----PEVRQSSFALLGDLTKACFQHVKPCIADFPILGTNLN---------------P--EF----------------------ISVCNNATWAIGEISIQ---GIE-QPYIPVLHQLVEIINR--PNTP-KTLLENTAITIGRL-GYVCPQEVAPLQQ----FI--RPWCTSLRNIRDNEEKDSAFRGICTISVNPSGVIQDFIFFCDAVASWINPKDDLRDFCKILHGFKNQVGDENWRRFSDQFPLPLKERLAAFYGV ------------NK---K-----LINQIAII---------------------------------CQNGYLKIKIPKEKEINIKNEEAYQAKNVGIRNLCIQQIKYYRNPNKTKPVVQNQTSTIQQNKQQNQQPQKSQNQVTSSFQVNN-QM--MQ--------------QKQSLVGQ-QIQSIVNELNLGEIQINKTQQDLKDFRKSKIIPQVNL--------------------NPSHSKRASNAWRQTLIASSSQNKINKDLQLSELGEQKLIN-MK----ETT-DQKEKIKYTKPLILQFTEYNPAYQYAEFNMESAIDLEDVPNSLDFEGELNDL--ISEEIIQQIKKNNFYSHLSFILAKRTKWGITQTQEKIMRHQLKPISNPLTNIADLKNIITYEDTKDAVQSIRSIIQQNNLYNDTSTQTQRPINYEDARTGKLDKQVSKQITNDQSQKLATVFSKYSLMNPRKASVRDTNSLEQIVLQQRKLTKLIQY-KKFS----------------------------------------------------------PSEKAINIFKTLLKCTGERKSRYNRHNNIIKLLTLCNEESIDLKEEV-YVQ--LCK-QLSANDKKESRIKYLKLFAIISSILPTTSRFYYPLLHFLYMRNTDTQIEQDERKCEQIQRGCIPTDTEIVMIENKKQILIRVYLLNEIPITVGVESYTSVKMLKQELLQKIGITQRHEYYGLMEVRNNIKKKRKETRFIDESERVMDVIAFWENEQYLFGSQLNSSFIHSNSKQNDEGIEQFNLQNYQDLEGLKIYLGIRILFSFQENDKEAISVFYTQL-SSEVLEGK-YAPLEEKDYLNLAALQLCIDFPRDVILKEISKRQSINA-DSG-------------SPQRTQSNFLSATSQSESGASQLKKLSYQDIEQIDEQDEYEQFIQNRNEILIDPVFYIPAKMYFQKDIQQWQHLILQNLVDNRLLKKEYTPSQAKLAYILYLSKQDLFMSTIFRDVKYQRIKIKTLNDTLSSKEKYEQMIQQNQQIPIKDEILLAINPSNILMCDSIGLNRPKRRVQIKNIQKFCCTEKEVIVISHDNTKHIFETPQYCREIAFLIRSYLRIF----Y--QK-QQQQQQQEKSQ--------------------------------------------------------------

ASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDKNGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCLLPQLTSPRLAVRKRTIIALGHLVMSCFVDLIEHLLSELSKNDSMSTTRTYIQCIAAISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFESFVRRCPK----------------------------EVYPHVSTIINICLKYLTYDMSWKVRRAAAKCLDAVVSTRHEMLPEFY---KTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVGETPLTMLQSQVPNIVKALHKQMK-EKSVKTR-QCCFNMLTELVNVLPGALTQHI-P-VLVPGIIFSLNDKSSSSN--LKIDALS-CLY--------VILCNHSPQVFHPHVQALVPPVVACVGDPFYKIT--SEALLVTQQLVKVIRPLDQP-------------------SSFDATPYIKDLFTCT-IKRLKAADIDQE---VKERAISCMGQIICNLGDNLGSDLPNTLQIFLERLKNEI---------TRLTTVKALTLIAGSPLKIDLRP-VLG-------------------E-GVPILASFLRKNQRALKLGTLSALDILIKN-YSDSLTAAMIDAVLDELPPLISESDMHVSQMAISFLTTLAKVYPSSLSKISGS-ILNELIGLVRSPLL-QGGALSAM---------LDFFQALVVTGTN---------------------------------NLGYMDLLRMLTGPVYSQ-THK--------Q--SYYSI----AKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLA-L-----------------------LSL-GEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEITSQPKRQYLLLH--------SLKEIISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVA-----E--CLGKLTLIDPE------T-LLPRLKGYLISGSSYARSSVVT-AVKF-TISDHPQPIDPLLKN--CIGDFLKTLEDPDLNVRRVALVTFNSAAHN---------------------------------------------------KPSLIRDLLDTVLPHLYNETKVRKELIREVEMGPFKHTVDDGLDIRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLSTLCPSAVLQRLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAEKSPLMSEFQSQISSNPELAA------------------------------------------------------------------------------------------------------------------------ ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NKK-LINQIAIIC--------------------------------------QNGYLKIKIPKEKEINIKNEEAYQAKNVGIRNLCIQQIK-YY-----------RNPNKTKPVVQNQTSTIQQNKQQNQQPQKSQNQVTSSFQVNNQMMQQKQSLVGQQIQSIVNE--LNLGEIQINKTQQDLKDFRKSKIIPQVNLNPSHSKRASNAWRQTLIASSSQNKINKDLQLSELGEQKLINMKETTDQKEKIKYTKPLILQFTEYNPAYQYAEFNMESAIDLEDVPNSLDFEGELNDLISEEIIQQIKKNNFYSHLSFILAKRTKWGITQTQEKIMRHQLKPISNPLTNIADLKNIITYEDTKDAVQSI-RSIIQQNNLYNDT--------------------ST-QTQRPINYEDARTGKLDKQVSKQITNDQSQKLATVFSKYSLMNPRKASVRDTNSLEQIVLQQRKLTKLIQYKKFSPSEKAINIFKTLLKCTGERKSRYNRHNNIIKLLTLCNEESI--D-LKE-EVYVQLCKQLSANDKKESRIKYLKLFAIISSILPTTSRFYYPLLHFLYMRNTDTQIEQDERKCEQIQ--RGCIPTDTEIVMIENKKQILIRVYLLNEIPITVGVESYTSVKMLKQELLQ-KIGITQRHEYYGLMEVRNNIKKKRKETRFIDES--E-R--VMDVIA-FW------ENEQYLF-GSQLNSSFIHSNSKQNDEGIEQFNLQN-YQDLEGLK--------I--Y--LG-I-RI---LF-SF--QEND-----KEA-ISV-FY---TQLSSEVLEGKYAPLEEKDYLNLAALQ--LC--ID-FPRDVILKEISK--RQSINADSGSPQRTQSNFLSATSQSESGASQLKKLSYQDIEQIDEQ-----D--EY-EQFI-QNRNEILIDPVFYI--PAK-----------------------------MYFQKDIQQWQHLILQNLVDNRLLKKEYTPSQAKLAYILYLSKQDLFMSTIFRDVK-------------------------------------------------------------------------------------------------------------------------------------------------------------------------YQRIKIKTLNDTLSSKEKYEQMIQQNQQIPIKDEILLAINPSNILMCDSIGLNRPKRRVQIKNIQKFCCTEKEVIVISHDNTKHIFETPQYCREIAFLIRSYLRIFYQKQQQQQQQEKSQ

PKIHYRKDYKPSGFIINQVT----------------L-NI----------NIHDQET--I--VR--SV--LDMDISKHNVGEDLV-------------------FDGVGLKINEISINNKKLVEGEEYTYDNEFLTIFSKF-VPK-SKFAFSSEVIIHPETNYALTG------------LYKSKNIIV--SQCEATGFRRITFFIDRPDMMAKYDVTVTADKEKYPVLLSNGDKVNEFEIPGGRHGARF-------------NDPPL-KPCYLFAVVAGDLKHLSATYITKYTKKKVELYVFSEEKYVSKLQWALECLKKSMAFDEDYFGLEYDLSRLNLVAVSDFNVGAMENKGLNIFNANSLLASKKNSIDFSYARILTVVGHEYFH-----------------------------QYTGNRVTL--R------DWFQLTLKEGLTVHRENLFSEEMTKTVTTRLSHVDLLRSVQFLEDSSPLSHPIRPESYVSMENFYTTTVYDKGSEVMRM-------------YLTILGEE---YYKKGFDIYIKKNDGNTATCED------------------------------------F--NYAMEQ-A-YKMKKA---D-NS-ANLNQ---------------------Y--LLWFSQSGTPHVSFKYNYDAEKKQ----YSIHVNQYTKPDENQKEKK------------PL--FIPISVGLINPENGK---EM-ISQTTL-EL--T--KE---SD--TFVFNNIAVKPIPS----LFRGFSAPVYIEDQLT----DE-----ERILLLKYD--S---D-AFVRYNSC--------TNIYMKQILMNYNEFLKAKNEKLE-S-----FQL---------TPV--NAQFIDAIKYLLED-PHA------DAGFKSYIVSLPQDRYIINFVSNLDTDVL-ADTKEYIYKQIGDKLNDVYYKMFKSLEAKADDLTYFNDESHVDFDQMNMRTLRNTLLS-L-L--------SK--A----QYPNILNEIIEHSKSPYPSNWLTSLSVS---A--Y---F-DKYFELYDKTYKLSKDDELLLQEWLKTVSRSDRKDIY----E---I--LKK-----LENEVLKDS-KNPNDIRAVYLPFTNNLR--RFHDISGKGYK------------LIA--EVITKTDKF------NPMVATQL-----------------CEPFKLWNKLDTK----RQE--LMLN--EMN-------------------TMLQEPQISNNLKE-YL----------------------------------L--RLTNK------------------ --------------------NKKLINQIAIICQNGYLKIKIPKEKEINIKNEEAYQAKNVGIRNLCIQQIKY-------------YRNPNKTKPVVQNQTSTIQQNKQQNQQPQKSQNQV---------------------TSS-FQVNN-----------------QMMQQKQSLVGQQ--------IQSIVNELNLGEIQINKTQQDLKDFRK----------------------------------SKIIPQVNLNPSHSKRASNAWRQTL-------------------------------------------------------------------------------------------------------------------IASSSQNKINKDLQLSELGEQKLINMKETTDQKEKIKYTKPLILQFTEYNPAYQYA-------------------------------------------EFNMES---------------------AIDLEDVPNSLDFE-------GELNDLISEEIIQQIKK--NNFYSHLSFILAKRTKWGITQTQEKIMRHQLKPISNPLTNIADLKNIITYEDTKDAVQSIRSIIQQNNLYNDTSTQTQRPINYEDARTGKLDKQVSKQITNDQSQKLATVF----------SKYSLMNPRKA------------SVRDTNSLEQIVL-QQRKLTKLIQ-------YKKF-SPSEKAINIFKTLLKCTGERKSRYNRHNNIIKLLTLCNEESIDLKEEVYVQLCKQLSANDKKESRIKYLKLFAIISSILPTTSRFYYPLLHFLYMRNTDTQIEQDERKCEQIQRGCIPTDTEIVMIENKKQILIRVYLLNEIPITVGVESYTSVKMLKQELLQKIGITQRHEYYGLMEVRNN-IKKKRKET------------RFIDESERVMDVIAFWENEQYLFGSQLNSSFIHSNS--K--QNDEGIEQFNLQNYQDLEGLKIYLGIRILFSFQENDKEAISVFYTQLSSEVLEGKYAPLEEKDYLNLAALQLCIDFPRDVILKEISKRQSINADSGS-PQRTQSNFLSATSQSESGASQLKKLSYQDIEQIDEQDEYEQFIQNRNEILIDPVFYIPAKMYF--QKD----------IQQWQHLILQNLV--DNRLLKKEYTPSQAKLAYILYLSKQDLFMSTIFRDVKYQRIKIKTLNDT------LSSKE--KYEQ--MIQ--QNQQIPIKDEILLAINPSNILMCDSIGLNRPKRRVQIKNIQKFCCTEKEVIVISHDNTKHIFETPQYCREIAFLIRSYLRIFYQKQQQQQQQEKSQ

CGVGFIANLRGKPDHTLV---------------EQALKALGCMEHRGGCSADNDSGDGAGVMTAIPRELLAQWFNTRNLPMPDGDRLGVGMVFLPQEPSAREVARAYVEEVVRLEKLTVLGWRE-VPVNSDVLGIQAKNNQPHIEQILV---------------TCPEG--CAGDELDRRLYIARSIIGKKLAEDFYVCSFSCRTIVYKGMVRSIILGEFYLDLKNPGYTSNFAVYHRRFSTNTMPKWPLAQPMRLLGHNGEINTLLGNINWMAA-------------REKELEVSGWTKAELEALTPIVNQANSDSYNLDSALELLVRT-GRSPL--EAAMILVPEAYKNQPALKDYPEIS------------------------DFHDYYSGLQEPWDGPALLVFSDGKIVGAGLDRNGLRPARYCITKDDYIVLGSEAGVVDLPEVDIVEKGRLAPGQMIAVDLAEQKILKNYQIKQQ-AAQKYPYGEWIKIQDAQTVLQQQAAFGYTAEDVEMVVVPMASQGKEPTFCMGDDTPLAVLSHKPRLLYDY------------------FKQ--RFAQVTNP------PIDPLRENLVMSLAMFLGKRGNLLEPKAE----------------KLRSPLVNEVELQAIKTGQLQV-AEVSTLYDLDGVNSLEDALTNLVKTAIATVQAGAEILVLTDRPNGAILTENQSFIPPLLAVGAVHHHLIRAGLRLKASLIVDTAQC---------------WSTHHFACLVGYGAS---AICPYLALESVRQWWLDEKTQKLMENGRLDRIDLPTALKNYRQSVEAGLFKILSKMGISLLASYHGAQIFEAIGLGAELVEYAFAGTTSRVGG------------LTIADVAGEVMVFHGMAFPEMAK--------------KLENFGFVNYRPGGEY---------HMN------SPEMS-KSLHKAVAAYYDHYE-----LYRQYLKDRPVTALRDLLDFNADQPAISLEEVESVESIVKRFCT---------------GGMSLGALSR-EAHETLAIAMNRLGAKSNSGEGGEDVVRYLTLDDVDSEG--------NSPTLPHLHGLQNGDTANSAIKQIASGRFGVTPEYLMSGKQLEIKMAQGAKPGEGGQLPG-----KKVSEYIAMLRRSKPGVTLISPPPHHDIYSIEDLAQLIYDLHQINPEAQVSVKLVAEIGIGTIAAGVAKANADIIQISGHDGGT----GASPLSSIKHAGSPWELGVTEVHRVLMENQLRDRVLLRA------DGGLKTGWDVVMAALM-GAEEYGFGSIAMIAE---GCIMARVCHTNNCPVGVATQQERLRQRFKGVPGQVVNFFYFIAEEVRSL-LAHLGYRSLDDIIGRTDLLKVRSDVQLSKTQNLTLDCLLNLPDTKQNRQ-------------------------------------------------WLNHEPV------------------------------H--SNGPV--LD-DDI---LADP----------DIQEAINHQTTAT--K-TY-RLVNTD-R-TVGTRLSGAIAKKYGNNGFEGNITLNFQGAAGQSFGAFNLDGMTLHLQGEAN-------D-YVGKGMNGGEIV-IVPHPQASFAPEDNVII----------------GNTCLYGATGGNLYANGRAGERFAVRNSVG---------------------KAVIE-GAGDHCCEYMTGGVIVVLGPVGRNVGAGMTGGLAYFLDEVGDLPEKINPEIITLQRITAS---KGEEQLKSLITAHVEHTGSPKGKAILANWSDYLGKFWQA-VP-PS--EK-DSPEAN--N-- ------------------NKKLINQIAIICQNGYLKIKIPKEKEINI--------K-NEEAYQAKNVGIRNLC----------IQQIKYYRNPN---------KT-KP---V---------VQ-NQT----------------------STIQQNKQQNQQPQKS----QNQVTSSFQVNNQMMQQKQS--------L-VGQ---QI---------------------------------------------QSIVNELNL--------------GEIQINKTQQDLKDFRKSKI--IPQVNLNPSHSKRA------SNAWRQTLIASS-SQNKINKDLQLSEL------------------GEQKLINMKETTDQKEKIKYTKPLILQFTEYNPAYQYAE----------------FN----------------------------------------M---------------------ES-----AIDLEDVP--------------------------------------------------------NSLDFEGELNDLISEEIIQ--QIKK------NNFYSHLSFILAKRTKWGI----------------TQTQEKIMRHQLKPISNPLTNIAD--LK--NI------ITYEDTKDA-----VQSIRSIIQQ-NN----------LYN----------------DTSTQTQR-----------------------PINYEDARTGKLDKQVSKQITNDQSQ----KLATVFSKYSLMNPRKASV-----------------RD-TNSLEQ---------------------------------------------------IVLQQRKLTKLIQYKKFSPSEKAIN----------IFKTLLKCTGERKSRYNRHNNIIKLLTLCNEESIDLKEEVYVQLCKQLSAND-KKES------------RIKYLKLFAIISSILPTTSRFYY--------------------------PLLHFLYMRNTDTQIEQDERKCEQ-IQR-------------------------------------GCIPTDTEIVMIENKKQIL-------------------------------------------------IRVYLLNEIPIT-----------------------------------------------------------------------------VGVES--------------------------------------YTSVKMLKQELLQKIGITQRHEYYGLMEVRNNIKK---KRKET-R-----------FIDE-----SERVMDVIAFWENEQYLFGS-QL--NS----SFIHSNSKQNDEG-------------------------------IEQFNLQNYQDLEGLKIYLGIRILFSFQENDKEAISVFYTQLSSEVLEGKYAPLEEKDYLNLAALQLCIDFPRDVILKEISKRQSINADSGSPQRTQSNF--LSAT---SQSESGASQLKKLSYQDIEQIDEQDEYEQFIQNRNEILIDPVFYIPAKMYFQKDIQQWQHLILQNLVD-----NR--------LLKKEYTPSQAKLAYILYLSKQD----L-FM----------------STIFRDVKYQRIKIKTLNDTLSSKEKYEQM----------------IQQNQQIPIKDEILLAINPSNILMCDSIGLNRPKRRVQIKNIQKF------------CCT---------------------------EKEVIVISHDNTKHIF--------E--TP--Q--YCREIAFLIRSYLRIFYQKQQQQQQQEKSQ

CGVGFIAAIDGKPRRSVVEK----------------GIEALKAVWHRGAVDADGKT-GDGAGIHVAVPQKFFKDHVKVIGHRAPDNKLAVGQVFLPRISLDAQEACRCIVETEILAFGYYIYGWRQVPINVDIIGEK-ANATRPEIEQIIVGNNKGV----------------SDEQFELDLYIIRRRIEKAVKGEQINDFYICSLSARSIIYKGMFLAEQLTTFYPDLLDERFESDFAIYHQRYSTNTFPTWPLAQPFRMLAHNGEINTVKGNVNW----------MKA--HETRMEHPAFGTHMQDLKPVIGVGLSDSGSLDTVFEVMVRA-GRTAPM-VKMMLVPQALTTTPDNHKA------------------------LIQYCNSVMEPWDGPAALAMTDGRWVVGGMDRNGLRPMRYTITTDGLIIGGSETGMVKIDETQVIEKGRLGPGEMIAVDLQSGKLYRDRELKDHLATLKPWDKWVQNTTHLDELVKTASLKGEPSDMDKAELRRRQQAFGLTMEDMELILHPMVEDGKEAIGSMGDDSPIAVLSDKYRG-----------------LHHF--FRQNFSQVTNP--PIDSLRERRVMSLKTRLGNLGNILDEDETQTRL---------------LQLESPVLTTAEFRAMRDYMGDTA---AEIDATFPVDGGPEALRDALRRIRQETEDAVRGGATHVILTDEAMGPARAAIPAILATGAVHTHLIRSNLRTFTSLNVRTAE-------------GLDTHYFAVLIGVGATT--VNAYLAQEAIAERHRRGLFGSMPLEKGMANY--KKAIDDGLLKIMSKMGISVISSYRGGGNFEAIGLSRALVAEHFPAMVSRISGI------------GLNGIQKKVLEQHATAYNEEV---------------VALPVGGFYRFRKSGD-----R--HGW------E--GGVIHTLQQAVTNDSYTTFK--------KYSEQVNKRPPMQLRDLLELRSTKAPVPVDEVESITAIRKRFIT-------------PGMSMGALSPE-AHGTLNVAMNRIGAKSDSGEGGEDPARFRP-------------DKNGDNWNSAIKQVASGRFGVTAEYLNQCRELEIKVAQGAKPGEGGQLPG------FKVTEMIARLRHSTPGVMLISPPPHHDIYSIEDLAQLIYDLKQINPDAKVTVKLVSRSGIGTIAAGVAKANADIILISGNSGGT----GASPQTSIKFAGLPWEMGLSEVHQVLTLNRLRHRVRLRTD-------GGLKTGRDIVIAAMLGAEEFGIGTASLIAMG----CIMVRQCHSNTCPVGVCVQDDKLRQKFVGTPEKVVNLFTFLAEEVREI-LAGLGFRSLNEVIGRTDLLHQVDLDLNPRLAQVDPG--------------------------------------------------GRN------------------------------------EVPDT--LD-ARI---VAD-----------ARPLFEEGEKMQL---AY-NARNTQ-R-AIGTRLSSMVTRKFGMFGLQPGHITIRLRGTAGQSLGAFAVQGIKLEVMGDAND---------YVGKGLSGGTIVVRPTTSSPLETNKNTII----------------GNTVLYGATAGKLFAAGQAGERFAVRNSGA--------------------TVVVE-GCGSNGCEYMTGGTAVILGRVGDNFAAGMTGGMAYVYDLDDSLPLYINDESVIFQRIEVG-HYESQLKHLIEEHVTETQSRFAAEILNDWAREVTKFWQVVPK-EM-LNR-LEVPVH--L-- --------------------NKKLINQIAIICQNGYLKIKIPKEKEIN--------IK-NEEAYQAKNVGIRNLCI----------QQIKYYRNPN-----KT--KP--V---------------VQ----------NQ------------------TSTIQQNKQQNQQPQKSQNQVTSSFQVNNQMMQQKQ--------SL-VG-----------------------------------------------Q--QIQSIVNELNL----------GEIQINKTQQD--LKDFRKSKII-PQVNLNPSHSKRA------SNAWRQTLIASS-SQNKINKDLQLSEL-------------GEQKLINMKETTDQKEKIKYTKPLILQFTEYNPAYQYA----------------EF----------------------------------------N---------------------------ME-SAIDLEDVP-------------------------------------------------------------------NSLDFEGELNDLISEEIIQ--QIKKNNFY-----SHLSFILAKRTKWGI--------------------TQTQEKIMRHQLKPISNPLTNIADL-KNI----------ITYEDTKDAVQS-I---RS-II--QQ--NN----------LYND----------TSTQTQRP-----------------------INYEDARTGKLDKQVSKQITNDQSQ--K--LATVFSKYSLMNPRKAS-------VRDTNSL--EQI----------------------------------------------------VLQQRKLTKLIQYKKFSPSEKAIN---------IFKTLLKCTGERKSRYNRHNNIIKLLTLCNEESIDLKEEVYVQLCKQLSANDKKES---------------RIKYLKLFAIISSILPTTSRFYYP----------------------------LLHFLYMRNTDTQIEQDERKCEQ-IQR----------------------------GCIPTDTEIVMIENKKQI---------------------------------------------LIRVYLLNEIPIT-----------------------------------------------------------------------------VGVES---------------------------------------YTSVKMLKQELLQKIGITQRHEYYGLMEVRNNIK----KKRKE-T---R-------FIDE-----SERVMDVIAFWENEQYLFGSQLN--SS----FIHSNSKQNDEG-------------------IEQFNLQNYQDLEGLKIYLGIRILFSFQENDKEAISVFYTQLSSEVLEGKYAPLEEKDYLNLAALQLCIDFPRDVILKEISKRQSINADSGSPQRTQSNF--LSAT--SQSESGASQLKKLSYQDIEQIDEQDEYEQFIQNRNEILIDPVFYIPAKMY--FQKDIQQWQHLILQN--------L-----VDNRLLKKEYTPSQAKLAYILYLSKQD----LFM----------------STIFRDVKYQRIKIKTLNDTLSSKEKYEQMI---------------QQNQQIPIKDEILLAINPSNILMCDSIGLNRPKRRVQIKNI----------------QKFC-----------------------C-TEKEVIVISHDNTKH-------IFETP----QYCREIAFLIRSYLRIFYQKQQQQQQQEKSQ

------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GMR--------------------------------------------------------------GL--AVF-ISDIRNCK----------SKEAEIKRINK-EL-----------------------------AN--IRSKFKGDKALDGYSKKKYVCKLL-----FI--FLL--GH---------------------------------------DI--D--FGHMEAVNLLSSNRY---TEKQIGYLFI-----SVLV------N-SNSELI-RLINNAIKNDLASR-----------NPTFMGLALHCIANVGSRE------MA------E--AFAGEIPKILVAGDTMDSVKQSAALCLLRLYRTSPDLVPMG-DWTSRVVH-LLNDQ-HLGVVTAATSLITTLAQKNPEEF-KTSVSLAV----SRLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDPAVRGRLTECLETILNKAQEPPKSK-K--VQHSNAKN-----------------------AVLFEAISLIIHHD---------SEPNLLVRACNQLGQFLQHR-ETN-LRYLALESMC----TLASSEFSHEAVKTHIETVINALKTERDVSV----RQRAVDLLYAMCDRSNAQQIVAEMLSYLETADYSIREEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPACHENLVKVGGYILGEFG NKKLINQIAIICQNGYLKIKIPKEKEINIKNEEAYQAKNVGIRNLCIQQIKYYRNPNKTKPVVQNQTSTIQQNKQQNQQPQKSQNQVTSSFQVNNQMMQQKQSLVGQQIQSIVNELNLGEIQINKTQQDLKDFRKSKIIPQVNLNPSHSKRASNAWRQTLIASSSQNKINKDLQLSELGEQKLINMKETTDQKEKIKYTKPLILQFTEYNPAYQYAEFNMESAIDLEDVPNSLDFEGELNDLISEEIIQQIKKNNFYSHLSFILAKRTKWGITQTQEKIMRHQLKPISNPLTNIADLKNIITYEDTKDAVQSIRSIIQQNNLYNDTSTQTQRPINYEDARTGKLDKQVSKQITNDQSQKLATVFSKYSLMNPRKASVRDTNSLEQIVLQQRKLTKLIQYKKF-SPSEKAINIFKTLLKCTGERKSRYNRHNNIIKLLTLCNEESIDLKEEVYVQLCKQLSANDKKESRIKYLKLFAIISSI--LPTTSRFYYPLLHFLYMRNTDTQIEQDERKCEQIQRGCIPTDTEIVMIENKKQILIRVYLLNEIPITVGVESYTSVKMLKQELLQKIGITQRHEYYGLMEVRNNIKKKRKETRFIDESERVMDVIAFWENEQYLFGSQLNSSFIHSNSKQNDEGIEQFNLQNYQDLEGLKIYLGIRILFSFQENDKEAISVFYTQLSSEVLE-GKYAPLEEKDYLNLAALQLCIDFPRDVILKEISKRQSINADSGSPQRTQSNFLSATSQSESGASQLKKLSYQDIEQIDEQDEYEQFIQNR--NE--ILI---DPVFYIPAKMYFQKDIQQWQ-H--LI-LQNLVDNRLLKKEYTPSQAKLAYILYLSKQDLFMSTIFRDVKYQRIKIKTLNDTLSSKEKYEQMIQQNQQIPIKDEILLAINPSN-I-LMCDSIGLNRPKRRVQIKNIQKFCCTEKEVIVI--SHDNTKHIFETPQYCREIAFLIRSYLRIFYQKQQQQQQQE---------------------------------K--SQ-------------------------------------------------------------------------

AAADGDDSLYPIA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VLIDELRNE---DV-QLRLNSIKKL--------------------STIALALGVER-TRSELLPFLTDTIYD--EDEVLLALAEQLG-------------------------------TFTTLVGGPEYVHCLLP----------------P--LESLATV----------EETVVRDKAVESLRA-ISHE-HS---PSDLE-------------AHFVPLVKRLAGGDWFTSRTSACGLFS-VC----YPRVSSAVKAELRQYFRNLCSDDTPMVRRAAASKLGEFAKVLEL-DNVK-S--E-IIPMFSNLASDEQDSVRLLAVEAC---------VNIAQLL---P---------------------------------QEDLEALVMPTLRQAAED-KS-W----RVRYMVAD-K----FTELQKAVGPEITKTD--LVPAFQNLMKDC---------------EAEVRAAASHKVKEFCE---------NLSADCRENVIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKD-ECP-E--VRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWR-VRLAIIE--Y---MP--LL-AGQ-LG--V-EFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA--------------------------------------------------------------------------------------------------------------------------------------------------------- -------------NKKLINQIAIICQNGYLKIKIPKEKEINIKNEEAYQAKNVGIRNLCIQQIKYYRNPNKTKPVVQNQTSTIQQNKQQNQQPQKSQNQVTSSFQVNNQMMQQKQSLVGQQIQSIVNELNLGEIQINKTQQDLKDFRKSKIIPQVNLNPSHSKRASNAWRQTLIASSSQNKINKDLQLSELGEQKLINMKETTDQKEKIKYTKPLILQFTEYNPAYQYAEFNMESAIDLEDVPN----SL---DFEGELNDLISEEIIQQIKKNNFYSHLSFILAKRTKWGITQTQEKIMRHQLKPISNPLTNIADLKN-IITYEDTKDAVQSIRSIIQQNNLYNDTSTQTQRPINYEDARTGKLDKQVSKQITNDQSQKLATVFSKYSLMNPRKASVRDTNSLEQIVLQQRKLTKLIQYKKFSPSEKAINIFKTLLKCTGERKSRY-NRHNNIIKLLTLCNEESIDLKEEV--YVQLCKQLSANDKKESRIKYLKLFAIISSILPT-TSRFYYPLLHFLYMRNTDTQIEQDERKCEQIQRGCIPTDTEIVMIENKKQILIRVYLLNEIPITVGVESYTSVKMLKQELLQKIGITQRHEYYGLMEVRNNIKKKRKETRFIDESERVMDVIAFWENEQYLFGSQLNSSFIHSNSKQNDEGIEQFNLQNYQDLEGLKIYLGIRILFSFQE--NDKEAISVFYTQLSSEVLEGKYAPLEEKDYLNLAALQLCIDF-PRDVILKEISKRQSINADSGSPQRTQSNFLSATSQSESGASQLKKLSYQDIEQIDEQD-----EYEQFIQNRNEILIDPVFYIPAKMYFQKDIQQWQHL--I---------------------LQNLVDNRL-------------------------------------------------------------------------------------------------------------------LKKEYTPSQAKLAYILYLSKQDLFMSTIFRDVKYQRIKIKTLNDTLSSKEKYEQMIQQNQQIPIKDEILLAINPSNILMCDSIGLNRPKRRVQIKNIQKFCCTEKEVIVISHDNTKHIFETPQYCREIAFLIRSYLRIFYQKQQQQQQQEKSQ

(a)	Residue pairs that are structurally aligned by TM-align program are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade.(more about the colors used)
(b)	Ranking of proteins is based on TM-score of the structural alignment between Model1 and the PDB structures in our template library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.

Function Prediction

Predicted EC Numbers

Rank	TM-score	RMSD^a	IDEN^a	Cov.	EC-Score	PDB Hit	EC No.
1	0.3870	8.79	0.04	0.60	0.3365	1ofeB	1.4.7.1

2	0.3513	7.65	0.05	0.49	0.3095	3b3bA	3.4.11.2

3	0.3689	9.09	0.03	0.59	0.3047	2vdcF	1.4.1.13

4	0.3320	8.69	0.04	0.51	0.2898	2e3tA	1.17.1.4

5	0.3281	8.81	0.04	0.51	0.2857	3bdjA	1.17.1.4

(a)	Ranking is based on EC-score, which is a confidence score for the Enzyme Classification (EC) Number prediction.
(b)	RMSD^a is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)	IDEN^a is percentage sequence identity in the structurally aligned region.
(d)	Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)	EC-Score is defined based on the C-score of the structure prediction and similarity of the model with known enzyme structures, as identified using both global and local structural alignment programs. The global similarity score uses TM-score, IDEN^a,RMSD^a and Cov. of the structural alignment by TM-align, while the local match compares the structural and chemical similarity of local spatial motifs in the model with known catalytic site of enzymes. A prediction with a EC-score >1.1 signifies a prediction with high confidence (upto 3 digit numbers of EC) and vice versa (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).

Predicted GO terms

Rank	TMscore	RMSD^a	IDEN^a	Cov.	PDB Hit	Fh-Score	Associated GO Terms
1	0.7634	3.00	0.08	0.82	2bptA	0.75	GO:0005515 GO:0008320 GO:0008565 GO:0000059 GO:0006612 GO:0010926 GO:0022607 GO:0034504 GO:0034621 GO:0034622 GO:0043623 GO:0051170 GO:0051292 GO:0051641 GO:0051649 GO:0070271 GO:0005622 GO:0005623 GO:0005634 GO:0005643 GO:0005737 GO:0012505 GO:0016020 GO:0016021 GO:0031975 GO:0043229 GO:0043234 GO:0044428 GO:0046930
2	0.6821	4.55	0.09	0.79	1uklB	0.69	GO:0008565 GO:0019904 GO:0000059 GO:0006605 GO:0006610 GO:0010926 GO:0022607 GO:0034504 GO:0034621 GO:0034622 GO:0043623 GO:0051170 GO:0051641 GO:0051649 GO:0070271 GO:0005622 GO:0005623 GO:0005634 GO:0005643 GO:0005737 GO:0012505 GO:0016020 GO:0016021 GO:0031975 GO:0043229 GO:0043234 GO:0044428 GO:0046930
3	0.5905	4.37	0.08	0.67	2qnaA	0.58	GO:0008139 GO:0008270 GO:0008565 GO:0019904 GO:0043169 GO:0000059 GO:0000060 GO:0006605 GO:0006607 GO:0006610 GO:0010926 GO:0022607 GO:0034504 GO:0034621 GO:0034622 GO:0043623 GO:0044419 GO:0051170 GO:0051641 GO:0051649 GO:0070271 GO:0005622 GO:0005623 GO:0005634 GO:0005643 GO:0005654 GO:0005737 GO:0005829 GO:0012505 GO:0016020 GO:0016021 GO:0031974 GO:0031975 GO:0043229 GO:0043234 GO:0044428 GO:0046930
4	0.5422	6.73	0.06	0.72	1z3hB	0.51	GO:0005049 GO:0008262 GO:0000059 GO:0000087 GO:0000278 GO:0006611 GO:0007067 GO:0008283 GO:0010926 GO:0022607 GO:0034504 GO:0034621 GO:0034622 GO:0043623 GO:0051168 GO:0051170 GO:0051301 GO:0051641 GO:0051649 GO:0070271 GO:0005622 GO:0005623 GO:0005634 GO:0005643 GO:0005737 GO:0012505 GO:0016020 GO:0016021 GO:0031975 GO:0043229 GO:0043234 GO:0044428 GO:0046930
5	0.4694	6.55	0.05	0.61	2z5oA	0.42	GO:0005515 GO:0008139 GO:0008565 GO:0000059 GO:0000060 GO:0006605 GO:0010926 GO:0022607 GO:0034504 GO:0034621 GO:0034622 GO:0043623 GO:0044419 GO:0051170 GO:0051641 GO:0051649 GO:0070271 GO:0005622 GO:0005623 GO:0005634 GO:0005643 GO:0005737 GO:0012505 GO:0016020 GO:0016021 GO:0031975 GO:0043229 GO:0043234 GO:0044428 GO:0046930
6	0.3504	2.34	0.09	0.37	2bptA	0.37	GO:0005515 GO:0008320 GO:0008565 GO:0000059 GO:0006612 GO:0010926 GO:0022607 GO:0034504 GO:0034621 GO:0034622 GO:0043623 GO:0051170 GO:0051292 GO:0051641 GO:0051649 GO:0070271 GO:0005622 GO:0005623 GO:0005634 GO:0005643 GO:0005737 GO:0012505 GO:0016020 GO:0016021 GO:0031975 GO:0043229 GO:0043234 GO:0044428 GO:0046930
7	0.3971	5.25	0.05	0.48	2pkgB	0.35	GO:0003823 GO:0008601 GO:0046982 GO:0009653 GO:0009987 GO:0010926 GO:0022610 GO:0040008 GO:0044237 GO:0048869 GO:0050789 GO:0050896 GO:0065007 GO:0000159 GO:0005622 GO:0005623 GO:0005625 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0015630 GO:0016020 GO:0043229
8	0.3609	6.97	0.06	0.49	2a0zA	0.34	GO:0003725 GO:0004888 GO:0005515 GO:0001816 GO:0002376 GO:0006955 GO:0009607 GO:0009987 GO:0032502 GO:0044237 GO:0050789 GO:0051704 GO:0065007 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005783 GO:0005789 GO:0005886 GO:0005887 GO:0010008 GO:0012505 GO:0016021 GO:0042175 GO:0043229 GO:0044432 GO:0044440 GO:0044444
9	0.3870	8.79	0.04	0.60	1ofeB	0.34	GO:0005506 GO:0005515 GO:0015930 GO:0016041 GO:0043169 GO:0051538 GO:0006536 GO:0006537 GO:0006541 GO:0008652 GO:0009058 GO:0009308 GO:0009309 GO:0009987 GO:0019752 GO:0044249 GO:0055114
10	0.3725	6.85	0.05	0.50	3ciyB	0.33	GO:0003725 GO:0004888 GO:0005515 GO:0001816 GO:0002376 GO:0006955 GO:0009607 GO:0009987 GO:0044237 GO:0050789 GO:0051704 GO:0065007 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005783 GO:0005789 GO:0010008 GO:0012505 GO:0016020 GO:0016021 GO:0042175 GO:0043229 GO:0044432 GO:0044440 GO:0044444

Consensus Prediction of Gene Ontology terms

Molecular Function    Biological Process Cellular Location

GO term GO-Score    GO term GO-Score GO term GO-Score

GO:0005515 0.467    GO:0009987 0.467    GO:0005737 0.467

GO:0005488 0.467    GO:0010926 0.367    GO:0044424 0.467

GO:0022892 0.332    GO:0044085 0.332    GO:0005623 0.467

GO:0005215 0.332    GO:0017038 0.332    GO:0044464 0.467

GO:0008565 0.332    GO:0051169 0.332    GO:0005622 0.467

GO:0019904 0.127    GO:0015031 0.332    GO:0016020 0.434

GO:0008320 0.112    GO:0051234 0.332    GO:0043227 0.434

GO:0022891 0.112    GO:0006605 0.332    GO:0043229 0.434

GO:0022857 0.112    GO:0051649 0.332    GO:0043231 0.434

GO:0005048 0.100    GO:0022607 0.332    GO:0043226 0.434

(a) Ranking in the first table is based on a function prediction score (Fh-score), which is calculated based on the C-score of the structure prediction and the TM-score, IDEN^a, RMSD^a and Cov. of the structural alignment by TM-align between the predicted model and the PDB structures (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).

(b) RMSD^a is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.

(c) IDEN^a is the percentage sequence identity in the structurally aligned region.

(d) Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.

(e) A consensus prediction of GO terms is derived from the structural analogs that have an Fh-score of >=1.0. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on the Fh-score of the template from which the GO term is derived. A prediction with a GO-score >0.5 signifies a prediction with high confidence and vice versa.

Predicted Binding Site

Binding site residues in the model:

GLN:	158	ILE:	161	ASN:	185	THR:	190	LYS:	193	GLU:	194	LYS:	195
ILE:	196	LYS:	197	TYR:	198	THR:	199	LEU:	202	TYR:	209	PRO:	211
ALA:	212	TYR:	213	GLN:	214	TYR:	215	ALA:	216	GLU:	217	PHE:	218
ASN:	219	MET:	220	SER:	244	GLU:	245	GLU:	246	ILE:	247	GLN:	250
ILE:	251	LYS:	252	LYS:	253	ASN:	254	ASN:	255	PHE:	256	TYR:	257
SER:	258

Identified analogs with similar binding site:

Rank PDB
Hit TM-score RMSD^a IDEN^a Cov. BS-score Download
Complex Binding site residues on the predicted model

1 2e1qA 0.3270 8.84 0.04 0.51 0.09 Download 158,161,185,190,193,194,195,196,197,198,199,202,209,211,212,213,214,215,216,217,218,219,220,244,245,246,247,250,251,252,253,254,255,256,257,258

2 1ulvA 0.2750 7.85 0.06 0.40 0.09 Download 588,687,690,729,777,842,847

(a) Ranking of the analogs in the table is based on their BS-score. BS-score is calculated by first finding the structural analogs of the predicted model based on their TM-score, IDEN^a and Cov. of the structural alignment. The ligand(s) in the analog structure are then transferred onto the model and the fitness of the ligand-model complex (BS-score) is calculated by comparing the local structure and sequence similarity in the binding site region. (For detail, see Kucukural, Szilagyi, Ambrish, and Zhang, Template based ligand binding site prediction on modeled protein structure, 2009, in preparation).

(b) A BS-score of >0.5 signifies a binding site prediction with high confidence and vice-versa.
(c) RMSD^a the RMSD between residues that are structurally aligned by TM-align.

(d) IDEN^a is the percentage sequence identity in the structurally aligned region.

(e) Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.

(f) The image shows the ligand-protein complex with the best BS-score. The ligand is depicted in magenta colored ball & stick, the predicted binding site residues interacting with the ligand are shown as transparent green spheres, while the N & C terminus in the model are marked by blue and red spheres respectively.

	Please cite following articles when you use the I-TASSER server:
1.	Yang Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9:40 (2008).
2.	Yang Zhang. Template-based modeling and free modeling by I-TASSER in CASP7. Proteins, 8: 108-117 (2007).
3.	Sitao Wu, Jeffrey Skolnick, Yang Zhang. Ab initio modeling of small proteins by iterative TASSER simulations. BMC Biology, 5:17 (2007).