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I-TASSER results for job id S34209

Submitted Sequence

>FGFR1
MWSWKCLLFWAVLVTATLCTARPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDD
VQSINWLRDGVQLAESNRTRITGEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSVNVSD
ALPSSEDDDDDDDSSSEEKETDNTKPNRMPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPS
SGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSIN
HTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKI
GPDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLE
ALEERPAVMTSPLYLEIIIYCTGAFLISCMVGSVIVYKMKSGTKKSDFHSQMAVHKLAKS
IPLRRQVTVSADSSASMNSGVLLVRPSRLSSSGTPMLAGVSEYELPEDPRWELPRDRLVL
GKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLKSDATEKDLSDLISEMEMMKMIGK
HKNIINLLGACTQDGPLYVIVEYASKGNLREYLQARRPPGLEYCYNPSHNPEEQLSSKDL
VSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFGLARDIHHIDYYKKTTNG
RLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHRMD
KPSNCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRIVALTSNQEYLDLSMPLDQYSPSF
PDTRSSTCSSGEDSVFSHEPLPEEPCLPRHPAQLANGGLKRR

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 620 640 660 680 700 720 740 760 780 800 820 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MWSWKCLLFWAVLVTATLCTARPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAESNRTRITGEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRMPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTSPLYLEIIIYCTGAFLISCMVGSVIVYKMKSGTKKSDFHSQMAVHKLAKSIPLRRQVTVSADSSASMNSGVLLVRPSRLSSSGTPMLAGVSEYELPEDPRWELPRDRLVLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLKSDATEKDLSDLISEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFGLARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRIVALTSNQEYLDLSMPLDQYSPSFPDTRSSTCSSGEDSVFSHEPLPEEPCLPRHPAQLANGGLKRR
Prediction	CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCSSSSSSCCCCSSSSSCCCCCCCCCSSSSSCCSSCCCCCSSSCCCCSSSSCCCCCCCCSSSSSSSSCCCCSSSSSSSSSSSCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCSCCCCCCCCCSSSSSCCCSSSSSSSCCCCCCCCSSSSSCCCCCCCCCSSCCSSSCCCCSSSSSCCCCCCCCCSSSSSSSCCCCCSSSSSSSSSSSCCCCCCSSCCCCCCCSSSSCCCCSSSSSSSCCCCCCSSSSSSSCCCCCCSSCCCCCCSSSSSSCCCCCCCCCSSSSSSSSCCCCCCCSSSSSSSSCCCCSSSSSSSSSSSCCCCCCCCCCCCCCSSSSCCCCCCCCCCCCCCCCCSSSCCCCCCCCCCCCCCCCCCCCCCCCSSSSCCCCCCCCCCCCCCCCCSSSSSSCCCCCCCCCCCCCCCCCCCCCCCCCCHHHSSSSSSSCCCCCCSSSSSSSSCCCCCCCCCSSSSSSSSSCCCCCHHHHHHHHHHHHHHHHHCCCCCSSSSSSSSCCCCCCSSSSSCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCSSCCCCCHHHSSSCCCCSSSSCCCCCCSSHHCCCSSSCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHCCCCCCSSSCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
Conf.Score	950456999999999882023778998654689989863268897589859997158999972299889968077985970263899886200128558999985877389999999945788877777775677664445665434566586015620255404686279779999865656887069988982836765120011100001799854664679329999991876189999888992268886750588662699579988999888479998169998656488654688995588753166401333256999926885467899999987897699999999977888898877898426514434666610436776036603568777886566651004665035731466788865456786457322524689965347663320026322004679799889568878779999995566778887468999995899998999999999999985388978711898990798758999678898179999862898643355777786687999999999999987999998688564454677769768987998046530141128667515797113222899899709624201156789999999856799989989999999997688689889989999999999841694349599999999999975448851255467667878888877888888777877788989998788899888999773479

Top 5 Models predicted by I-TASSER


Download Model 1	Download Model 2	Download Model 3	Download Model 4	Download Model 5
C-score=-2.35	C-score=-2.66	C-score=-2.78	C-score=-2.81	C-score=-2.62

Estimated accuracy of Model1: 0.44±0.14 (TM-score) 14.4±3.8Å (RMSD) (Read more about C-score of generated models)

Top 10 templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov.

Norm.
Z-score

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560                 580                 600                 620                 640                 660                 680                 700                 720                 740                 760                 780                 800                 820
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Sec.Str
Seq

CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCSSSSSSCCCCSSSSSCCCCCCCCCSSSSSCCSSCCCCCSSSCCCCSSSSCCCCCCCCSSSSSSSSCCCCSSSSSSSSSSSCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCSCCCCCCCCCSSSSSCCCSSSSSSSCCCCCCCCSSSSSCCCCCCCCCSSCCSSSCCCCSSSSSCCCCCCCCCSSSSSSSCCCCCSSSSSSSSSSSCCCCCCSSCCCCCCCSSSSCCCCSSSSSSSCCCCCCSSSSSSSCCCCCCSSCCCCCCSSSSSSCCCCCCCCCSSSSSSSSCCCCCCCSSSSSSSSCCCCSSSSSSSSSSSCCCCCCCCCCCCCCSSSSCCCCCCCCCCCCCCCCCSSSCCCCCCCCCCCCCCCCCCCCCCCCSSSSCCCCCCCCCCCCCCCCCSSSSSSCCCCCCCCCCCCCCCCCCCCCCCCCCHHHSSSSSSSCCCCCCSSSSSSSSCCCCCCCCCSSSSSSSSSCCCCCHHHHHHHHHHHHHHHHHCCCCCSSSSSSSSCCCCCCSSSSSCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCSSCCCCCHHHSSSCCCCSSSSCCCCCCSSHHCCCSSSCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHCCCCCCSSSCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
MWSWKCLLFWAVLVTATLCTARPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAESNRTRITGEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRMPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTSPLYLEIIIYCTGAFLISCMVGSVIVYKMKSGTKKSDFHSQMAVHKLAKSIPLRRQVTVSADSSASMNSGVLLVRPSRLSSSGTPMLAGVSEYELPEDPRWELPRDRLVLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLKSDATEKDLSDLISEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFGLARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRIVALTSNQEYLDLSMPLDQYSPSFPDTRSSTCSSGEDSVFSHEPLPEEPCLPRHPAQLANGGLKRR

2psqA

0.89

0.31

0.35

1.37

Download

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LPEDPKWEFPRDKLTLGKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVKMLKDDATEKDLSDLVSEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLRARRPP-----------PEEQMTFKDLVSCTYQLARGMEYLASQKCIHRDLAARNVLVTENNVMKIADFGLARDINNIDYYKKTTNGRLPVKWMAPEALFDRVYTHQSDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLLKEGHRMDKPANCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRILTLTT------------------------------------------------------------

1opkA

0.30

0.19

0.53

1.86

Download

----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NLFVALYDSGDNTLSITKGEKLRVLGYNHNGE-WCEAQTKNGQGPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGIGSFLVRESPGQRSISLRYEGRVYHYRINTASDGKVSSESRFNTLAEL--HHHSTVADGLITTLHYPAPKRNKPTIYGVS----PNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKK-------YSLTVAVKTLKEDTME--VEEFLKEAAVMKEI-KHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNR--------------QEVSAVVLLYMATQISSAMEYLEKKNFIHRNLAARNCLVGENHLVKVADFGLSRLM-TGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGK-------------------------------------------------

1ir3A

0.35

0.15

0.36

2.53

Download

------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SSVFVPDEWEVSREKITLLRELGQGSFGMVYEGNARDIIKGEA--ETRVAVKTVNESASLRERIEFLNEASVMKGF-TCHHVVRLLGVVSKGQPTLVVMELMAHGDLKSYLRSLRPEAENN------PGRPPPTLQEMIQMAAEIADGMAYLNAKKFVHRDLAARNCMVAHDFTVKIGDFGMTRDI---ETDRKGGKGLLPVRWMAPESLKDGVFTTSSDMWSFGVVLWEITSLAEQPYQGLSNEQVLKFVMDGGYLDQPDNCPERVTDLMRMCWQFNPKMRPTFLEIVNLLKDDLHPSFPEV-------SFFHSEENK-----------------------------------------

1opkA

0.30

0.19

0.53

3.37

Download

NLF--------------------------VALYDFVASGDNTLSITKGEKLRVLGYNHNGE----WCE---AQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSSGINGS----FLVRESE------------------------------------------------------------------------------------------------------SSPGQRSISLRY-------------------------------------------------EGRVYHYR---------------------------------------INTASDGKLYV-------------------------------------------------------------------------------------SSESRFNTSTVADGLITTLHYPAPKRNKPTIYGVSP----NYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYS-------LTVAVKTLKEDTMEVE--EFLKEAAVMKEI-KHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNR--------------QEVSAVVLLYMATQISSAMEYLEKKNFIHRNLAARNCLVGENHLVKVADFGLSR-LMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELG-------------------------------------------------K

1pkgA

0.51

0.18

0.35

2.39

Download

-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NVIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLIKSDAA--MTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTIGGPTLVITEYCCYGDLLNFLRRKRD---------SFICSKALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIKNDSNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPDSKFYKMIKEGFRMLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQLIEKQ-----------------------------------------------------------------

2psqA

0.89

0.31

0.35

1.45

Download

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LPEDPKWEFPRDKLTLGKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVKMLKDDATEKDLSDLVSEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLRARRPP-----------PEEQMTFKDLVSCTYQLARGMEYLASQKCIHRDLAARNVLVTENNVMKIADFGLARDINNIDYYKKTTNGRLPVKWMAPEALFDRVYTHQSDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLLKEGHRMDKPANCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRILTLTT------------------------------------------------------------

1fgkA

0.99

0.34

10.56

Download

-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELPEDPRWELPRDRLVLGKPLG-----QVVLAEAIGLP----NRVTKVAVKMLKSDATEKDLSDLISEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLQARRPP--------EE----QLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFGLA-DIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRIVALTS------------------------------------------------------------

2psqA

0.89

0.31

0.35

1.68

Download

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LPEDPKWEFPRDKLTLGKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVKMLKDDATEKDLSDLVSEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLRARRPP-----------PEEQMTFKDLVSCTYQLARGMEYLASQKCIHRDLAARNVLVTENNVMKIADFGLARDINNIDYYKKTTNGRLPVKWMAPEALFDRVYTHQSDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLLKEGHRMDKPANCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRILTLTT------------------------------------------------------------

1rjbA

0.49

0.19

0.36

2.24

Download

-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YESQLQMVQVTGSSDNEYFYVDFREYEYDLKWEFPRENLEFGKVLGSGAFGKVMNATAYGISKTGV--SIQVAVKMLKER------EALMSELKMMTQLGSHENIVNLLGACTLSGPIYLIFEYCCYGDLLNYLRSKRE--------------KFLTFEDLLCFAYQVAKGMEFLEFKSCVHRDLAARNVLVTHGKVVKICDFGLARDIMSDSNYVVRGNARLPVKWMAPESLFEGIYTIKSDVWSYGILLWEIFSLGVNPYPGIPDANFYKLIQNGFKMDQPFYATEEIYIIMQSCWAFDSRKRPSFPNLTSFLGCQL----------------------------------------------------------------

1pkgA

0.51

0.18

0.35

1.74

Download

-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NVIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLIKSDAA--MTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTIGGPTLVITEYCCYGDLLNFLRRKRD---------SFICSKALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIKNDSNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPDSKFYKMIKEGFRMLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQLIEKQ-----------------------------------------------------------------

(a)	All the residues are colored in black; however, those residues in template which are identical to the residue in the query sequence are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade. (more about the colors used)
(b)	Rank of templates represents the top ten threading templates used by I-TASSER.
(c)	Ident1 is the percentage sequence identity of the templates in the threading aligned region with the query sequence.
(d)	Ident2 is the percentage sequence identity of the whole template chains with query sequence.
(e)	Cov. represents the coverage of the threading alignment and is equal to the number of aligned residues divided by the length of query protein.
(f)	Norm. Z-score is the normalized Z-score of the threading alignments. Alignment with a Normalized Z-score >1 mean a good alignment and vice versa.
(g)	Download Align. provides the 3D structure of the aligned regions of the threading templates.
(h)	The top 10 alignments reported above (in order of their ranking) are from the following threading programs:
	1: HHSEARCH 2: MUSTER 3: SP3 4: PROSPECT2 5: PPA-I 6: HHSEARCH I 7: FUGUE 8: HHSEARCH II 9: SPARKS 10: MUSTER

10 proteins in PDB which are structurally closest to the first I-TASSER model (identified by TM-align)

Rank	TM-score	RMSD^a	IDEN^a	Cov.	PDB Hit
1	0.5294	0.96	0.29	0.53	1opkA Model1

2	0.4610	3.26	0.28	0.51	1ad5A Model1

3	0.4506	3.53	0.26	0.50	1fmkA Model1

4	0.3507	4.50	0.19	0.40	2ozoA Model1

5	0.3413	4.53	0.11	0.39	3c4wA Model1

6	0.3405	4.37	0.12	0.39	2acxA Model1

7	0.3342	4.96	0.11	0.40	1omwA Model1

8	0.3287	2.91	0.34	0.35	1k9aA Model1

9	0.3244	2.57	0.31	0.34	1m14A Model1

10	0.3241	2.57	0.53	0.34	2ivsA Model1

Structural alignment using TM-align
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGE-WCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAEL-VHHHSTVADGLITTLHYPAPKRNKPTIYGVSP----NYDKWEMERTDITMKHKLGGGQYGEVYEGVWKK-------YSLTVAVKTLKEDTM--EVEEFLKEAAVMKE-IKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECN---R-----------QEVSAVVLLYMATQISSAMEYLEKKNFIHRNLAARNCLVGENHLVKVADFGLSRLM-TGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGK------------------------------------------------- MWSWKCLLFWAVLVTATLCTARPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAESNRTRITGEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRMPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGV--NTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSH--HSAWLTVLEALEERPAVMTSPLYLEIIIYCT-GAFLISCM---VGSVIVYKMKSGTKKSDFHSQMAVHKLA--KSIPLRRQVTVSADSSASMNSGVLLVRPSRLSSSGTPMLAGVSEYELPEDPRWELPRDRLVLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLKSDATEKDLSDLISEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFGLARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRIVALTSNQEYLDLSMPLDQYSPSFPDTRSSTCSSGEDSVFSHEPLPEEPCLPRHPAQLANGGLKRR

-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------E-D-----IIVVALYDYEAIHHEDLSFQKGDQMVVLEES--G-EWWKARSLATRKEGYIPSNYVARVDSLETEEWFFKGISRKDAERQLLAPGNMLGSFMIRDSETTKGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRSTFSTLQEL-VDHYKKGNDGLCQKLSVPCMSSKPQKP-WEK-------DAWEIPRESLKLEKKLGAGQFGEVWMATY-N-------KHTKVAVKTMK--PGS---MSVEAFLAEANVMKTLQHDKLVKLHAVVT-KEPIYIITEFMAKGSLLDFLKSDEG-SK-QPL-----------PK-LIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLA----------RV-GAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPEELYNIMMRCWKNRPEERPTFEYIQSVLDDFYTAT--------------------------------------------ESQQQ-------QP--- MWSWKCLLFWAVLVTATLCTARPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAESNRTRITGEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRMPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKT-AG-VNTTDKEMEVLHLRNVSFEDAGEYTCLAG-N-SIGLSH-H-SAWLTVLEALEERPAVMTSPLYLEIII-Y-CTG-AFLISCM---V-GSVIVYKMKS---GTKKSDFHSQMA--VHKLAK-SIPLRRQVTVSADSSASMNSGVLLVRPSRLSSSGTPMLAGVSEYELPEDPRWELPRDRLVLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLKSDATEKDLSDLISEMEMMKMI-GKH-KNIINLLGACTQDGPLYVIVEYASKGNLREYLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFGLARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRIVALTSNQEYLDLSMPLDQYSPSFPDTRSSTCSSGEDSVFSHEPLPEEPCLPRHPAQLANGGLKRR

-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------M-V------TTFVALYDYESRTETDLSFKKGERLQIVNNT--EGDWWLAHSLSTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQL-VAYYSKHADGLCHRLTTVCPTSKPQTQGLAKD-------AWEIPRESLRLEVKLGQGCFGEVWMGTW-N-------GTTRVAIKTLK--PGTM--S-PEAFLQEAQVMKKLRHEKLVQLYAVVSE-EPIYIVTEYMSKGSLLDFLKGETG-KY-LRL-----------PQ-LVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLA----------R----FPIK-WTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPES-LHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTE---------------------------------------------PQ-----QP-GENL-- MWSWKCLLFWAVLVTATLCTARPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAESNRTRITGEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRMPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQIL-KTAGV--NTTDKEMEVLHLRNVSFEDAGEYTCLAG-N-SIGLSH-H-SAWLTVLEALEERPAVMTSPLYLEIIIY--CTG-AFLISCM---VGSVIVYKMKS-----GTKKSDFHSQMAV-HKLAK-SIPLRRQVTVSADSSASMNSGVLLVRPSRLSSSGTPMLAGVSEYELPEDPRWELPRDRLVLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLKSDATEKDLSDLISEMEMMKMI-GKHK-NIINLLGACTQDGPLYVIVEYASKGNLREYLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFGLARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPV-EELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRIVALTSNQEYLDLSMPLDQYSPSFPDTRSSTCSSGEDSVFSHEPLPEEPCLPRHPAQLANGGLK-RR

PDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCEFYSRDPDGLPCNLRKPCNRPSGLEPQPGVFDCLRDAMVR---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DYVRQTWKLEGEALEQAIISQAPQVEK-LIATTAHERMPWYHSSLTREEAERKLYSGAQTDGKFLLRPRKEQGTYALSLIYGKTVYHYLISQDK------------AGKYCIPEGTK----FDTLWQLVEYLKLKADGLIYCLKEAC--PN--------------MDTSVFESPFSDPEEL----KDKKLFLKRDNLLIADIELGCGNFGSVRQGVY--RMR-K--KQIDVAIKVLK--QGTEKADTEEMMREAQIMHQ-LDNPYIVRLIGVCQ-AEALMLVMEMAGGGPLHKFLV-----GKR-E---------EIPVSNVAELLHQVSMGMKYLEEKNFVHRNLAARNVLLVNRHYAKISDFGLSK---------AL--G--PLKWYAPECINFRKFSSRSDVWSYGVTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPELYALMSDCWIYKWEDRPDFLTVEQRMRACYY-SLASKVEGGSALEVA------------------------------------------------------ ---------------------------------------------------------------------------------------------------------------------------MWSWKCLLFWAVLVTATLCTARPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAESNRTRITGEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRMPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERP----AV-MTSPLYLEIIIYCTGAFLI-----------------------------------------------------------SCMVGSVIVYKMK----------SGTKKSD-FHS-QMAVHKLAKSIPLRRQVTVSADSSASMNSGVLLVRPSRLSSSGTPMLAGVSEYELPEDPRWELPRDRLVLG-KPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLKSDATEKD--LSDLISEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFGLARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRIVALTSNQEYLD-------LSMPLDQYSPSFPDTRSSTCSSGEDSVFSHEPLPEEPCLPRHPAQLANGGLKRR

RDRKYLA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RLKLPPLSKCEALRESLDLG-----------FEGMCLEQPIGKRLFQQFLR---THEQHGP------ALQLWKDIED--Y-DTA----D----------DALRPQKAQALRAAYLEPQAQLFCSFLDAETVA------------------RARAGAGDGLFQPLLRAVLAHLGQAPFQEFLDSLYFLR------------------------------------------FLQW---KWLEAQPMGEDWFLDFRVLG-RGGFGEVFACQMK----A--TGKLYACKKLNKKRLKKRKGYQGAMVEKKILAK-VHSRFIVSLAYAFETKTDLCLVMTIMNGGDIRYHIYNV-DEDNPGF-----------QEPRAIFYTAQIVSGLEHLHQRNIIYRDLKPENVLLDDDGNVRISDLGLAVELKAGQTKTKGY---A-GTPGFMAPELLLGEEYDFSVDYFALGVTLYEMIA-ARGPFRARGEKVENKELKQRVLE-QAVTYPDKFSPASKDFCEALLQKDPEKRLGFRDGSCDGLRTHPLFRDISWRQLEAGMLTPPFVPDQDVGAFSTVKGVAFEKADTEFFQEFASGTCPIPWQEEMIETGVFGDLNVWRP----------------------------------------------------------- -------MWSWKCLLFWAVLVTATLCTARPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAESNRTRITGEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRMPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNT----------------TDKEMEVLHLRNVSFEDAGE--YTC-LA---GNSI------GLSHHSAWLT-VLEALEERPAVMTSPLYLEIIIYCTGAFLISCMVGSV-----------------------IVYKMKSGTKKSDFHSQMAVHKLA--------------------------------KSIPLRRQVTVSADSSASMNSGVLLVRPSRLSSSGTPMLAGVSE--YELPEDPRWELPRDRLVLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLKSDAT--EKDLSDLISEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFG-LARDI-HHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPG--V-PV-EELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVPSQRPTFK--QLVEDLDRIVALTSN--------------------------------------------------------------------QEYLDLSMPLDQYSPSFPDTRSSTCSSGEDSVFSHEPLPEEPCLPRHPAQLANGGLKRR

KAR-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KGKSKKWRQMLQFPHISQCEELRLSLERD-----------YHSLCERNPIGRLLFREFCATRPELSR----------CVAFLDGV---AEYEV-----------------TP-----DDK-----------RKACG--RNLT------QNFLSHTGPDLIPEVPRQLVTNCTQRLEQGPCKDLFQELTRLTHEYLSVAPFADYLDSIYFNR------------------------------------FLQW---KWLERQPVTKNTFRQYRVLG-KGGFGEVCACQVR----A--TGKMYACKKLEKKRIKKRKGEAMALNEKQILEK-VNSRFVVSLAYAYETKDALCLVLTLMNGGDLKFHIYH--MGQAGFP------------EARAVFYAAEICCGLEDLHRERIVYRDLKPENILLDDHGHIRISDLGLAVHVPEGQTIKGR---V-GTVGYMAPEVVKNERYTFSPDWWALGCLLYEMIAGQSPFQQRKKKIKREEVERLVKE-VPEEYSERFSPQARSLCSQLLCKDPAERLGCRGGSAREVKEHPLFKKLNFKRLGAGMLEPPFKPDPQAIYCEPTDQDFYQKFATGSVPIPWQNEMVETECFQELNVFGLDGS----------------------------------------------------------- ---MWSWKCLLFWAVLVTATLCTARPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAESNRTRITGEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRMPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTA---GV--NT-----------------TDKEMEVLHLRNVSFEDAGE---YTCLA----------GNSIGLSHHSAWLTVLEALEERPAVMTSPLYLEIIIYCTGAFLISCMVGSVIVYKMKSGTKKSDFHSQMAVHKL-AKSIPLRR-------------------------------------------------------------QVTVSADSSASMNSGVLLVRPSRLSSSGTPMLAGVSE---YELPEDPRWELPRDRLVLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLKSDAT--EKDLSDLISEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFG-LARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPYP--GVPV-EELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVPSQRPTF--KQLVEDLDRIVALTSN---------------------------------------------------------------QEYLDLSMPLDQYSPSFPDTRSSTCSSGEDSVFSHEPLPEEPCLPRHPAQLANGGLKRR

SK-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KILLPEPSIRSVMQKYLEDRGEV-----------TFEKIFSQKLGYLLFRDFC-LKHLEEAKP------LVEFYEEIKK--YEKLE----T----------EEERLVCSREIFDTYIMKELLACSHPFSKSAIEH-----------------VQGHLVKKQVPPD------LFQPYIEEICQNLRGDVFQKFIESDKFTRFCQ----------------------------------WKN----VELNIHLTMNDFSVHRIIG-RGGFGEVYGCRKA----D-TGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQH---GVFSE------------A-DMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHAS---V-GTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKL-LRGHSPFRQHKTKDKHEIDRMTLTMAVELPDSF-SPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKTKNKQLGHEEDYALGKDCIMHGYMSKMGWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFVLQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKP---------------------------------------------------------- --MWSWKCLLFWAVLVTATLCTARPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAESNRTRITGEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRMPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVN-------------------TTDKEMEVLHLRNVSFEDAGE-YTC-LAG-NSI-------GLSHHSAWLT-VLEALEERPAVMTSPLYLEIIIYCTGAFLISCMVGS-------------------------VIVYKMKSGTKKSDFHSQMAVHKLAK----SIPLRRQ-------------------------------VTVSADSSASMNSGVLLVRPSRLSSSGTPMLAGVSE-YELPEDPRWELPRDRLVLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLKSDAT-EKDLSDLISEMEMMKMIGKH-K-NIINLLGACTQDGPLYVIVEYASKGNLREYLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFGLARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTH-QSDVWSFGVLLWEIFTLGGSPYPGVP--V-EELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVPSQRPTF-KQLVEDLDRIVALTSNQ-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EYLDLSMPLDQYSPSFPDTRSSTCSSGEDSVFSHEPLPEEPCLPRHPAQLANGGLKRR

ASWPSGTECIAKYNFHGTA---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EQDLPFCK--------------GDVLT----------IVAVTKDPNW------YKA------KNKVGREGIIPANY---------------------------------------------------------------------------------------------------------VQKREGVKAGTKLSLMPWFHGKITREQAERLLYPPETGLFLVRESTNYPGDYTLCVSCEGKVEHYRIMYHASKLSIDEEVYFENLMQLVEHYTTDADGLCTRLIKPKVMEGTVAAQDEFYRSGWALNMKELKLLQTIGKGEFGDVMLGDY-R--------GNKVAVKCI---KNDATAQAFLAEASVMTQ-LRHSNLVQLLGVIVEEKGGLYIVTEYMAKGSLVDYLRSRG---R----------S-VLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVSEDNVAKVSDFGLTKEA-----S-----K-LPVKWTAPEALREKKFSTKSDVWSFGILLWEIYSFGRVPYPRIPLKDVVPRVEKGYKMDAPDGCPPAVYDVMKNCWHLDAATRPTFLQLREQLEHIRTHELHL---------------------------------------------------------- -------------------MWSWKCLLFWAVLVTATLCTARPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAESNRTRITGEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRMPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSK------IGPDNLPYVQILKTAGVNTTDKEMEVLHLRNVS------FEDAGEYTCLAGNSIGLSHHSA-------WLTVLEALEERPAVMTSPLYLEIIIYCTGAFLISCMVGSVIVYKMKSGTKKSDFHSQMAVHKLAKSIPLRRQVTVSADSSASMNSGVLLVRPSRLSSSGTPMLAGVSEYELP---------------------------------------------------------------------------------------------------------------E-DPRWELPRDRLVLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLKSDATEKDLSDLISEMEMMKMIGKHKNIINLLGACTQD-GPLYVIVEYASKGNLREYLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFGLARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRIVALTSNQEYLDLSMPLDQYSPSFPDTRSSTCSSGEDSVFSHEPLPEEPCLPRHPAQLANGGLKRR

GEAP--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NQA-LLRILKETEFKKIKVLGSGAFGTVYKGLW-I-PEGE-KVKIPVAIKELREATSPKANKEILDEAYVMASV-DNPHVCRLLGICL-TSTVQLITQLMPFGCLLDYVREHKD---------------NIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEG-GKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMAR--DPQRYLV-IQGDDEEDMDDVVDADEYLIPQ--------------------------------------------------- ----MWSWKCLLFWAVLVTATLCTARPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAESNRTRITGEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRMPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTSPLYLEIIIYCTGAFLISCMVGSVIVYKMKSGTKKSDFHSQMAVHKLAKSIPLRRQVTVSADSSASMNSGVLLVRPSRLSSSGTPMLAGVSEYELPEDPRWELPRDRLVLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLKSDATEKDLSDLISEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFG-LARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRIVALTSNQEYLDLSM--------------------PLDQYSPSFPDTRSSTCSSGEDSVFSHEPLPEEPCLPRHPAQLANGGLKRR

E----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------D-PKWEFPRKNLVLGKTLG-EGEFGKVVKATAFHLKG-R-AGYTTVAVKMLKENASPSELRDLLSEFNVLKQ-VNHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLRESRKVGPG-Y-------LRALTMGDLISFAWQISQGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRDVYE-EDSYVKRSQGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMV-KR------------------------------------------------------------ -MWSWKCLLFWAVLVTATLCTARPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAESNRTRITGEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRMPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTSPLYLEIIIYCTGAFLISCMVGSVIVYKMKSGTKKSDFHSQMAVHKLAKSIPLRRQVTVSADSSASMNSGVLLVRPSRLSSSGTPMLAGVSEYELPEDPRWELPRDRLVLGKPLGEGCF-GQVVLAEAIGLDKDKPNRVTKVAVKMLKSDATEKDLSDLISEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFG-LARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRIVALTSNQEYLDLSMPLDQYSPSFPDTRSSTCSSGEDSVFSHEPLPEEPCLPRHPAQLANGGLKRR

(a)	Residue pairs that are structurally aligned by TM-align program are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade.(more about the colors used)
(b)	Ranking of proteins is based on TM-score of the structural alignment between Model1 and the PDB structures in our template library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.

Function Prediction

Predicted EC Numbers

Rank	TM-score	RMSD^a	IDEN^a	Cov.	EC-Score	PDB Hit	EC No.
1	0.3200	2.63	0.93	0.34	1.1456	3c4fA	2.7.10.1

2	0.3196	2.48	0.83	0.34	1.0431	2pvyA	2.7.10.1

3	0.5329	1.47	0.28	0.54	0.8304	2fo0A	2.7.10.2

4	0.3176	2.92	0.53	0.34	0.7356	2p2hA	2.7.10.1

5	0.3192	2.33	0.52	0.33	0.7308	2ivtA	2.7.10.1

(a)	Ranking is based on EC-score, which is a confidence score for the Enzyme Classification (EC) Number prediction.
(b)	RMSD^a is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)	IDEN^a is percentage sequence identity in the structurally aligned region.
(d)	Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)	EC-Score is defined based on the C-score of the structure prediction and similarity of the model with known enzyme structures, as identified using both global and local structural alignment programs. The global similarity score uses TM-score, IDEN^a,RMSD^a and Cov. of the structural alignment by TM-align, while the local match compares the structural and chemical similarity of local spatial motifs in the model with known catalytic site of enzymes. A prediction with a EC-score >1.1 signifies a prediction with high confidence (upto 3 digit numbers of EC) and vice versa (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).

Predicted GO terms

Rank	TMscore	RMSD^a	IDEN^a	Cov.	PDB Hit	Fh-Score	Associated GO Terms
1	0.3200	2.66	0.93	0.34	3c4fB	1.10	GO:0004713 GO:0005007 GO:0005515 GO:0005524 GO:0008201 GO:0016301 GO:0030246 GO:0030554 GO:0007154 GO:0007275 GO:0009653 GO:0016043 GO:0040007 GO:0043170 GO:0044237 GO:0044238 GO:0048856 GO:0048869 GO:0050793 GO:0050794 GO:0065008 GO:0005623 GO:0005624 GO:0005886 GO:0005887 GO:0016021
2	0.3189	2.76	0.53	0.34	2p2iA	0.69	GO:0004713 GO:0005021 GO:0005524 GO:0016301 GO:0019838 GO:0030554 GO:0009987 GO:0010926 GO:0032501 GO:0032502 GO:0044237 GO:0048870 GO:0050789 GO:0050896 GO:0051179 GO:0051704 GO:0065007 GO:0005623 GO:0005886 GO:0005887 GO:0016021
3	0.1923	2.52	0.95	0.20	3c4fB	0.67	GO:0004713 GO:0005007 GO:0005515 GO:0005524 GO:0008201 GO:0016301 GO:0030246 GO:0030554 GO:0007154 GO:0007275 GO:0009653 GO:0016043 GO:0040007 GO:0043170 GO:0044237 GO:0044238 GO:0048856 GO:0048869 GO:0050793 GO:0050794 GO:0065008 GO:0005623 GO:0005624 GO:0005886 GO:0005887 GO:0016021
4	0.3130	2.25	0.52	0.33	2ivvA	0.67	GO:0004713 GO:0004714 GO:0005509 GO:0005515 GO:0005524 GO:0016301 GO:0030554 GO:0043169 GO:0000165 GO:0001657 GO:0001755 GO:0001838 GO:0006468 GO:0006928 GO:0007154 GO:0007156 GO:0007169 GO:0007497 GO:0009653 GO:0009887 GO:0014031 GO:0016310 GO:0016331 GO:0016477 GO:0022610 GO:0034960 GO:0042551 GO:0043412 GO:0048468 GO:0048469 GO:0048484 GO:0048598 GO:0048856 GO:0060429 GO:0065007 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0016021 GO:0043229 GO:0044444
5	0.4609	3.44	0.27	0.51	2srcA	0.63	GO:0004715 GO:0005070 GO:0005524 GO:0016301 GO:0030554 GO:0030674 GO:0042169 GO:0060090 GO:0006468 GO:0007154 GO:0007172 GO:0007243 GO:0007265 GO:0010926 GO:0016310 GO:0022607 GO:0034621 GO:0034622 GO:0034960 GO:0043412 GO:0043623 GO:0044419 GO:0065007 GO:0070271 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005901 GO:0045121
6	0.2983	2.43	0.46	0.31	1rjbA	0.58	GO:0004713 GO:0005021 GO:0005524 GO:0016301 GO:0030554 GO:0002376 GO:0006793 GO:0007154 GO:0007165 GO:0007275 GO:0042127 GO:0043283 GO:0044238 GO:0044260 GO:0048518 GO:0048522 GO:0048856 GO:0065007 GO:0005623 GO:0005886 GO:0005887 GO:0016021
7	0.3089	2.86	0.42	0.33	2ogvA	0.57	GO:0004713 GO:0005011 GO:0005515 GO:0005524 GO:0016301 GO:0030554 GO:0006468 GO:0007154 GO:0007169 GO:0007275 GO:0008283 GO:0016310 GO:0032502 GO:0034960 GO:0043412 GO:0065007 GO:0005623 GO:0005886 GO:0005887 GO:0016021
8	0.3182	1.72	0.39	0.33	3dk7B	0.55	GO:0000287 GO:0004715 GO:0005524 GO:0016301 GO:0019904 GO:0030145 GO:0030554 GO:0043169 GO:0007049 GO:0007155 GO:0016310 GO:0018108 GO:0018212 GO:0022610 GO:0030029 GO:0030036 GO:0034960 GO:0043412 GO:0051726 GO:0065007 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005856 GO:0043229
9	0.3019	1.80	0.41	0.31	3d7uC	0.54	GO:0004715 GO:0005524 GO:0008022 GO:0016301 GO:0030554 GO:0006793 GO:0042127 GO:0043283 GO:0044238 GO:0044260 GO:0048519 GO:0050794 GO:0065007 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911
10	0.2793	2.79	0.46	0.30	2p4iB	0.54	GO:0004713 GO:0004714 GO:0005524 GO:0016301 GO:0030554 GO:0006468 GO:0006928 GO:0007160 GO:0007169 GO:0007267 GO:0009887 GO:0010926 GO:0016310 GO:0016337 GO:0022610 GO:0030334 GO:0032526 GO:0034960 GO:0042127 GO:0042221 GO:0043412 GO:0045765 GO:0048646 GO:0048856 GO:0065007 GO:0005623 GO:0005886 GO:0005887 GO:0016021

Consensus Prediction of Gene Ontology terms

Molecular Function    Biological Process Cellular Location

GO term GO-Score    GO term GO-Score GO term GO-Score

GO:0004713 0.654    GO:0044237 0.654    GO:0005623 0.654

GO:0016301 0.654    GO:0009987 0.654    GO:0005886 0.599

GO:0030554 0.654    GO:0065007 0.654    GO:0044459 0.532

GO:0005524 0.654    GO:0050794 0.585    GO:0031224 0.482

GO:0004871 0.545    GO:0044238 0.585    GO:0016021 0.482

GO:0005515 0.542    GO:0048856 0.533    GO:0031226 0.415

GO:0004888 0.482    GO:0044260 0.520    GO:0005887 0.415

GO:0004714 0.482    GO:0032501 0.482    GO:0005737 0.239

GO:0019199 0.482    GO:0007154 0.476    GO:0044424 0.239

GO:0004872 0.482    GO:0007275 0.413    GO:0005622 0.239

(a) Ranking in the first table is based on a function prediction score (Fh-score), which is calculated based on the C-score of the structure prediction and the TM-score, IDEN^a, RMSD^a and Cov. of the structural alignment by TM-align between the predicted model and the PDB structures (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).

(b) RMSD^a is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.

(c) IDEN^a is the percentage sequence identity in the structurally aligned region.

(d) Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.

(e) A consensus prediction of GO terms is derived from the structural analogs that have an Fh-score of >=1.0. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on the Fh-score of the template from which the GO term is derived. A prediction with a GO-score >0.5 signifies a prediction with high confidence and vice versa.

Predicted Binding Site

Binding site residues in the model:

LEU:	484	GLY:	485	PHE:	489	VAL:	492	LEU:	494	ALA:	512	VAL:	513
LYS:	514	GLU:	531	MET:	535	ILE:	545	VAL:	559	VAL:	561	GLU:	562
TYR:	563	ALA:	564	SER:	565	LYS:	566	GLY:	567	LEU:	630	ALA:	640
ASP:	641	PHE:	642

Identified analogs with similar binding site:

Rank PDB
Hit TM-score RMSD^a IDEN^a Cov. BS-score Download
Complex Binding site residues on the predicted model

1 2zvaA 0.3023 2.30 0.41 0.32 0.33 Download 484,485,489,492,494,512,513,514,531,535,545,559,561,562,563,564,565,566,567,630,640,641,642

2 1opjB 0.3216 1.63 0.36 0.33 0.30 Download 484,489,492,512,513,514,531,534,535,538,544,545,559,561,563,564,565,567,614,619,620,621,630,639,640,641,642,643,644,645,646

3 2oh4A 0.3133 2.82 0.52 0.33 0.28 Download 484,489,492,494,512,514,531,535,538,544,545,561,562,563,564,565,566,567,614,621,630,639,640,641,642,643,644

4 2hk5A 0.3080 1.93 0.39 0.32 0.24 Download 484,485,488,489,492,494,512,513,514,531,535,545,559,561,562,563,564,565,567,568,627,628,630,640,641,642

5 3c4fA 0.3200 2.63 0.93 0.34 0.23 Download 484,489,492,494,512,514,535,545,561,562,563,564,565,567,630,640,641,642

6 2oo8X 0.2792 2.79 0.45 0.30 0.22 Download 484,485,489,492,512,513,514,527,531,535,538,544,545,559,561,562,563,564,614,621,630,639,640,641,642,643,644,645

7 1t46A 0.3146 2.55 0.47 0.33 0.21 Download 484,489,492,494,512,513,514,531,534,535,538,544,545,559,561,562,563,564,565,567,614,619,620,621,630,640,641,642,643,644,645,646

8 3dpkA 0.2958 2.05 0.49 0.31 0.14 Download 483,484,485,486,488,489,492,494,512,514,531,535,545,547,561,562,563,564,565,566,567,568,627,628,630,640,641,642

9 1opjB 0.3216 1.63 0.36 0.33 0.14 Download 581,582,583,584,597,600,601,690,694,723,724,725,729,754,767,771

10 2r4bA 0.3109 2.76 0.34 0.33 0.14 Download 463,484,485,489,492,512,513,514,531,535,545,546,547,559,560,561,562,563,564,567,568,630,640,641,642

(a) Ranking of the analogs in the table is based on their BS-score. BS-score is calculated by first finding the structural analogs of the predicted model based on their TM-score, IDEN^a and Cov. of the structural alignment. The ligand(s) in the analog structure are then transferred onto the model and the fitness of the ligand-model complex (BS-score) is calculated by comparing the local structure and sequence similarity in the binding site region. (For detail, see Kucukural, Szilagyi, Ambrish, and Zhang, Template based ligand binding site prediction on modeled protein structure, 2009, in preparation).

(b) A BS-score of >0.5 signifies a binding site prediction with high confidence and vice-versa.
(c) RMSD^a the RMSD between residues that are structurally aligned by TM-align.

(d) IDEN^a is the percentage sequence identity in the structurally aligned region.

(e) Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.

(f) The image shows the ligand-protein complex with the best BS-score. The ligand is depicted in magenta colored ball & stick, the predicted binding site residues interacting with the ligand are shown as transparent green spheres, while the N & C terminus in the model are marked by blue and red spheres respectively.

	Please cite following articles when you use the I-TASSER server:
1.	Yang Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9:40 (2008).
2.	Yang Zhang. Template-based modeling and free modeling by I-TASSER in CASP7. Proteins, 8: 108-117 (2007).
3.	Sitao Wu, Jeffrey Skolnick, Yang Zhang. Ab initio modeling of small proteins by iterative TASSER simulations. BMC Biology, 5:17 (2007).