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I-TASSER results for job id S34211

  Submitted Sequence

>VHL
MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPR
PVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFR
DAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDI
VRSLYEDLEDHPNVQKDLERLTQERIAHQRMGD

  Predicted Secondary Structure

Sequence                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200
                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |              
MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQRMGD
PredictionCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCSSSSSSSCCCCCSSSSSSSCCCCCSSSCCCCCCCCSSSSSSCCCCSSSSSSCCCCCCSSSCCSSSSSCCCCCCCCSSSSSSSSCCCCHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHCHCHHHHHHHHHHHHHHHHHCCC
Conf.Score986457854335672335664458656787532655578755687778841110667789864147888448999985899768799988899884654328998688522057547887437886136605676634667789747999961140289999999998617410650457277999988683099999999999997765059

  Top 5 Models predicted by I-TASSER

Download Model 1 Download Model 2 Download Model 3 Download Model 4 Download Model 5
C-score=-1.75
 C-score=-2.16
 C-score=-2.58
 C-score=-2.55
 C-score=-2.95
 
Estimated accuracy of Model1: 0.50±0.15 (TM-score)    9.4±4.6Å (RMSD)    (Read more about C-score of generated models)

  Top 10 templates used by I-TASSER

RankPDB
Hit		Iden1Iden2Cov.Norm.
Z-score		Download
Align.		                   20                  40                  60                  80                 100                 120                 140                 160                 180                 200
                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |             
Sec.Str
Seq		CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCSSSSSSSCCCCCSSSSSSSCCCCCSSSCCCCCCCCSSSSSSCCCCSSSSSSCCCCCCSSSCCSSSSSCCCCCCCCSSSSSSSSCCCCHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHCHCHHHHHHHHHHHHHHHHHCCC
MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQRMGD
11lm8V 1.00 0.70 0.70 2.89Download -----------------------------------------------------------RPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQ----
21lm8A 1.00 0.70 0.7010.97Download -----------------------------------------------------------RPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQ----
31lm8V 1.00 0.70 0.70 2.99Download -----------------------------------------------------------RPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQ----
41lm8V 0.99 0.70 0.70 3.13Download -----------------------------------------------------------RPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIA----HQ
51lm8V 1.00 0.70 0.70 4.22Download -----------------------------------------------------------RPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQ----
61lm8A 1.00 0.70 0.70 9.77Download -----------------------------------------------------------RPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQ----
71vcbC 1.00 0.67 0.67 3.66Download --------------------------------------------------------------LRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQE---------
81lm8A 1.00 0.70 0.70 8.72Download -----------------------------------------------------------RPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQ----
91lm8V 1.00 0.70 0.70 1.47Download -----------------------------------------------------------RPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQ----
101eo9B 0.12 0.20 0.91 0.60Download QRNTEDHPPAYAPGYKTSVLRSPKNALISIAETLSEVTAPH-FSADKFGPKDNDLILNYAKDGLPIGERVIVHGYVRDQFGRLVEVWQANASGNDQYIGAMDPNFCGRMLTDDNGYYVFRT------IKPGP----YPWRNRINEWRPAHIHFSLIA-DGWAQRLISQFYFEGDIDSCPI-------LKTIPS-EQQRRALIALEDKSNFIEA
(a)All the residues are colored in black; however, those residues in template which are identical to the residue in the query sequence are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade. (more about the colors used)
(b)Rank of templates represents the top ten threading templates used by I-TASSER.
(c)Ident1 is the percentage sequence identity of the templates in the threading aligned region with the query sequence.
(d)Ident2 is the percentage sequence identity of the whole template chains with query sequence.
(e)Cov. represents the coverage of the threading alignment and is equal to the number of aligned residues divided by the length of query protein.
(f)Norm. Z-score is the normalized Z-score of the threading alignments. Alignment with a Normalized Z-score >1 mean a good alignment and vice versa.
(g)Download Align. provides the 3D structure of the aligned regions of the threading templates.
(h)The top 10 alignments reported above (in order of their ranking) are from the following threading programs:
       1: MUSTER   2: HHSEARCH   3: SP3   4: PROSPECT2   5: PPA-I   6: HHSEARCH I   7: FUGUE   8: HHSEARCH II   9: SPARKS   10: MUSTER   

  10 proteins in PDB which are structurally closest to the first I-TASSER model (identified by TM-align)

RankTM-scoreRMSDaIDENaCov.PDB
Hit
10.6798 1.07 0.99 0.701lm8V
Model1
 
20.4871 4.81 0.03 0.751nkgA
Model1
 
30.4467 5.65 0.06 0.771b41A
Model1
 
40.4436 5.61 0.04 0.752nupB
Model1
 
50.4278 5.36 0.08 0.691fgjA
Model1
 
60.4272 5.28 0.06 0.691gz4A
Model1
 
70.4271 5.72 0.05 0.723b4wA
Model1
 
80.4268 5.71 0.04 0.731kv7A
Model1
 
90.4229 6.02 0.05 0.753biwA
Model1
 
100.4175 5.74 0.05 0.711a4zA
Model1
 
Structural alignment using TM-align
 
-----------------------------------------------------------RPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAH--Q-- 
MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQRMGD 
 
AFGITTSSSAYVIDTNAPNQLKFTVSRSSCDITSIIHYGTELQYSSQGSHIGSGLGSATVTATQSGDYIKVTCVTDTLTQYMVVHNGDPIIHMAT-YITAEPSIGELRFIARLNSDLLPNE------------------EPFGDVSTTADGTAIEGSDVFLVGSETRSKFYSSERFIDDQRHCIAGDAHRVCMILNQYESSSGGPFHRDINSNNGGSYNALYWYMNSGHVQ-TE-SYRMGLHGPYSMYFSRSGTPSTSIDTSFFADLDIKG-----YVAASGRGKVAGTASGADSSMDWVVHWYNDAA--QYWTYTSSSGSFTSPAMKPGTYTMVYYQG--EY-AVA---T-SSVTVSAG--STTTKNISGSVKTGTTIFKIGEWDGQPTGFRNAANQLRMHPSDSRMSSWGPLTYTVGSSALTDFPMAVFKSVNNPVTIKFTATSAQTGAATL-RI-GTTLSFAGGRPQATINSYTGSAPAAPTNLDSRGVTRGAYRGLGEVYDVSIPSGTIVAGTNTITINVISGSSGDTYLSPNFIFDCVELFQ--------------- 
-----------------------------------------------------------------------------MPRRAE---------NWDEAEVGA---EEAGV------------EEYGPEEDGGEESGAEESGPEE-S-------------------------GPE----------------------------------ELG----------------AEEEMEAGRPRP---------------------------------VLRSVNS---REPSQVIFCNRS-P-RVVLPVWLNFDGEPQPYPTLPPGTGRRI-HS-YRGHLWLFRDAGTHDGLLVNQTELFVPSLN--VDGQPIFANITLPVYT-LKERCLQV--VRSLVK-P------------ENYRR-LDIV-------------------RSLY--------------EDLEDHPN-----------------------------------VQKDL---------------------------------------------ERLTQERIAHQRMGD 
 
DAE-LLVTVRGG-----RLRGIRLKTPGGPVS-AFLGIPFAEPPMGPRRFLPPEPKQPWSGVVDATTFQ--SVC-YQYVDTLYPGFEGTEMWNPNRELSEDCLYLNV---------WTPYPRPTSPTPVLVW---------IYGG-----GFYSGASSLDVYDGRFLVQAERTVLVSMNYRVGAFGFLALPGSREAPGNVGLLDQRLALQWVQENVAAFGGDPTSVTLFGESA----GAASVGM---HLLS-------PPSRGLFHRAVLQSGAPNGPWATVGMGEARRRATQLAHLVGCPNDTELVACLRTRPAQVLVNHEWHVLPQESVFRFSFVPVVDGDFLSDTPEALINAGDFHGLQVLVGVVKDEG-SYFLVYGAPGFSK-D-NESLISRAEFLAGVRVGVPQVSDLAAEAVVLHYTDWLHPEDPARLREALSDVVGDHNVVCPVAQLAGRLAAQGARVYAYVFEHRASTLSWPLWMGVPHGYEIEFIFGIPLDPSRNYTAEEKIFAQRLMRYWANFARTGDPNEPPKAPQWPPYTAGAQQYVSLDLRPLEVRRGLRAQACAFWNRFLPKLLSAT 
M--PR-------RAENWDEAEVGAEE----A-GVEEYGPEEDGGEE-----------------------SGA--EESGPEE--SGPEE-LG----------------AEEEMEAGRPRP--------VLRSVNSREPSQVIF---CNRSPRVVLPVW----LNFD---------GEPQPYPT---LPPG--------TGR--RI--HS---YR---------G--H-LWLFRDAGTHDGLLVNQTELFV--PSLNVDGQPIF-A---------NITL---------------------------------------------------------------------------------------PVYTLKERCLQVVRSL---VKPENYRRLD-------------------------------------IVR-SL-------------------------------------------YEDLEDHP-N-VQKDLE----------------R--LTQERIA-----HQRMGD------------------------------------------------ 
 
EGLRVVNLLQERNMLPSTPLKPPVPNLHEDIQKLNCNPELFRCTLTSIPQTQALLNKAKLPLGLLLHPFKDLVQLPVVTSSTIVRCRSCRTYINPFVSFLDQRRWKCNLCYRVNDVPEEFLEPHRRPEVQNATIEFMAPSEYMLRPPQPPVYLFVFDVSHNAVETGYLNSVCQSLLDNLDLLPGNTRTKIGFITFDSTIHFYGLQESLSQPQMLIVSDIEDVFIPMPENLLVNLNESKELVQDLLKTLPQMFTKTLE--TQSALGPALQAAFKLMSPTGGRMSVFQTQLPTLG----VGALKPREEPNHRSSAKM---TPSTDFYKKLALDCSGQQVAVDLFLLSG-----QYSDLASLGCISRYSAGSVYYYPSYHHQHNPVQVQKLQKELQRYLTRK----IGFEAVMRIRCTKGLSIHTFHGN-----------FFVRSTDLLSLP-N---VNPDAGYAVQMSVEESLTDTQLVSFQ------SALL---YTSSKGE---RRIRVHTLCLPVVSTLNDVFLG--ADVQAISGLLANMAVDRSMTASLSDARDALVNAVIDSLSAYRSSVPGLMVPFSLRLFPLFVLALLKQKSFQTGTNARLDERIFAMCQVKNQPLVYLMLTTHPSLYRVDNLSDEGALNISDRTIPQPPILQLSVEKLSRDGAFLMDAGSVLMLWVGKNCTQNFLSQVLGVQNYASIPQPMTDLPELDTPESARIIAFISWLREQRPFFPILYVIRDESPMKANFLQNMIEDRTESALSYYEFLLHIQQQVNK------ 
-------------------------------------------------------------------------------------------------------------------------------------------------------------MP-----------------------------------RRAENWDE-----------------------------------------AE--VG--------AEEAG-V--EE-YGP--EEDG---------------GEESGA----------------EESGPEE------------------------SGPEELGA-------------------EEE----M--EAGRP--RPVLRSV--NSREPSQVIFCNRS------P-RVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLW-LFRDAG----------------THDGLLVNQ-TELFVPSLNVDGQPIFANITL--------------PVYTLKERC-LQV-VR--S---L--VKPENYRRL-DI---------------------VRSLYEDLE-D-H--------------PNVQK--D---L---E-----------------------------------------------RLT---------------------------------------------------Q--ER-IA-------------------------------------------HQRMGD 
 
DISTVPDETYDALKLDRGKATPKETYEALVK---RYK--DP--------AHGAGKGTMGDYWEPIAISIYMDPNTFYKPPVSPKEVAERKDCVECHSDETPVWVRAWKRSTHANLDKIRNLKSDDPLYYKKGKLEEVENNLRSMGKLGEKETLKEVGCIDCHVDVNKKDKADHTKDIRMPTADTCGTCHLREFAERESERDTMVWPNGQWPAGR-PSHALDYTANIETTVWATMPQREVAEGC-------TMCHT-----NQNKCDNCHTRHEFSAAESR--------------------KPEACATCHSG--VDH---------------NNWEAYTMSKHGKLAEMNRDKWNWEVRLKDAFSKGGQ-NAPTCAACHMEYEGEYTHNITRKTRWANY-PFVPGIAENITSDWSEARLDSWVLTCTQCHSERFARSYLDLMDKGTLEGLAKYQEANAIVHKMYEDGTLTGQKTNRPNPPEPEKPGFGIFTQLFWSKGNNPASLELKVLEMGENNLAKMHVGLAHVNPGGWTYTEGWGPMNRAYVEIQDEYTKMQELSALQARVN 
-------------------------------MPRR--AENWDEAEVGAEEAGVEEY--G--PEEDG--G-------EES------------------------------------------------------------------------------------------------GAEESG--PEE--S-GPE-ELGA-EEEMEAG--RPRP--VL-------R---SV-NSREPS-QVIFC-NRSPRVVLPVWLNFDGEPQPY-PT-------------LPPGTGRRIHSYRGHLWLFRDAGTHDG-LLVNQTELFVPSLNVDGQPIFANITLPV-Y-TLKERCLQ--V--VRSLVKPENYRRLDIVRSLYEDLEDHP--------NVQ-KDLERLTQERI---A------------------------------------------HQRMGD-------------------------------------------------------------------------------------------------------------- 
 
EKGKPLLN------------------------PRTNKGAFTLQERQLGLQGLLPPKIETQDIQALRFHRNLKKTSPLEK-----------------------------------------YIYIGI--QERNEKL-------FYRILQDDIESLPIVYTPTVGLACSQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGGIPVGKLCLYT---ACAGIRPD---------RCLPVCIDVGTDNIALLKDPFYGLYQKRDRTQQYDDLIDEFKAITDRYGRNTL-----IQFEDFGNHNAFRFLRKYREKYCTFNDDIQGTAAVALAGLLAAQKVISKPISEHKILFLGAGEAALGIANLIVSVENGLSEQEAQKKIWFDKYGLLVKGRKAKIDSYQEPFTHSAPESIPDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAASINERPVIFALSNPTAQAECTAEEAYTLTEGRCLFASGSPFGP-VKLTD------GRVFTPGQGNNVYIFPGVALAVILCNTRHISDSVFLEAAKALTSQLTDEELAQGRLYPPLANIQEVSINIAIKVTEYLYANKAFRYPEPEDKAKYVKERTWRSEYDSLLPDVYEWP 
--------MPRRAENWDEAEVGAEEAGVEEYGPEEDGG-------------EES-G-A---EE--SGPE----------ESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVV-L--PVW------LNFDGEPQPYPT---------L----P--PG--T------GRR-----------------IHSYRGHL------------------------WLFRD-AGTHD------GLLVNQTELFVP----------------------------------------SLNV------DGQPIFANIT---------------------LP---V--YT-----------------------------------------------------------------------------------------------------------------------------L-------------------------KERCLQVVRSLV---KPENYRRLDIV--------------------------------------------RSLYEDLEDHP--NVQKDLERLTQERIAHQRMGD--------------------------------- 
 
SATEYDKLFIG-------GKWTKPSTSDVIEVRCPATGEYVGKVPMAAAADVDAAVAAARAAFDNGPWPSTPPHERAAVIAAAVKMLAERKD--------------LFTKLLAAETGQPPTIIETMHWMGSMGAMNYFAGAADKVTWTETRTGSYGQSIVSREPVGVV-------G-A-I---VAWNVPLFLAVNKIAPALLAGCTIVLKPAAETPLTANALAEVFAEVGLPEGVLSVVPGGIETGQALTSNPDIDMFTFTGSSAV-GREVGRRAAEMLKP---------------CTLEL----G--GKSAAIILEDVDLAAAIPMMVFSGVMNAGQGCVNQTRILAPRSRYDEIVAAVTNFVTALPVGPPSDPAAQIGPLISEKQRTRVEGYIAKGIEEGARLVCGGGRPEGLDNGFFIQPTVFADVDNKMTIAQEEIFGPVLAIIPYDTEEDAIAIANDSVYGLAGSVWTTDVPKGIKISQQIRTGTYGINWYAFDPGSPFGGYKNSGIGRENGPEGVEHFT--QQ--KSVLLPMGYTV- 
-----------MPRRAENWD-----------------------------------------E---AEV--G----AEEA--GV---------EEYGPEEDGGEESGAEESGP----------E---ESGP-EEL-GA-EEEME-AGRP-------------RPVLRSVNSREPSQVIFCNRSPRVV-----LP--VW--LN-FDGEPQPYPTLP--------------------------PGTGR--RIHSYRGHLWLFRDAGT--HDG-LLVNQT--ELFVPSLNVDGQPIFANITLPVYTLKERCLQVV-----------------------------RSLVK-------------------------------------------------------------------------------------------------------------------------PENYRRL-----D-IVRS-LYEDLE-----------DHP--------NVQ--KDLERLTQERIAHQRMG-------D 
 
RPTLPIPDL----------------LTTDARNRIQLTIGAGQSTFGGKTATTWG--------------YNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHWHGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLVVIEDDEILKLMLPKQWGIDDVPVIV------QDKKFSADGQIDYQLDVMTAAVGWF-GDTLLT-------------NGAI---YPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVLMGERFEVLVEVNDN--KPFDLVTLPV-SQMGMAIAPFDKPHPVMRIQPIAISASGALPDTLSSLPALPSLEGLTVRKLQ----LS-MDPMLDMM--GMQMLMEKYGDQAMAGMDFHHA-NKINGQAFDMNKPMFAAAKGQYERW-VISGVGDMM-LHPFHIHG-TQFR-ILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDTGMMLGFTV--------- 
---------MPRRAENWDEAEVGAEEAGV-------------------------EEYGPEEDGGEESGAEESGP-----------------E-----ESGPE---------EL--------------GAEEEMEAG---------------------------R-PRP-VLRSVNSREP------SQVIFCN------------------------RSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSY-RGHLWLFRDAGT-HDGLLVN----------------------QTE---------LFVPSLNVDGQPIFANITLPVYTL-------------------------------------------------KERCL-QVV------RSL-----------------VKP-ENYR--RLD-----------------IVRSLY----EDLEDHPN-VQKD-LE---------RLTQE------------------------------------------------RIAHQRMGD 
 
D-------------------PLV--TTNFGK-IR-G---IKKELNNEILGPVIQFLGVPYAAPPTGEHRFQPPEPPSPWSDIRNATQFAPVCPQNIIDGRLPEVMLPVWFTNNLDVVSSYVQDQSEDCLYLNIYVPTIR--DS---GGP-KPVM----------VYIHGGSYMEGTGNLYDGSVLASY-----GNVIVITVNYRLGVLGFLSTGDQAAKGNYGLLDLIQALRWTSENIGFFGGDPLRITVFGSGA-----GGSCVNLLTLSHY--SEKGL-----FQ-RAIAQSGTALSSWAVSFQPAKYARILATKVGCNVSDTVELVECLQKKPYKELVDQDVQPARYHIAFGPVIDGDVIPDDPQILMEQGEFLNYDIMLGVNQGEGLKFVENIVDSDDGVSASDFDFAVSNFVDNLYGVLRETIKFMYTDWADRHNPETRRKTLLALFTDHQWVAPAVATADLHSNFGSPTYFYAFYHHCQTDQVPAWADAAHGDEVPYVLGIPMIGPTELFPCNF--S---------KNDVMLSAVVMTYWTNFAKTGDPNQPVEVAWTRYSQKDQLYLHIGLKPRVKEHYRANKVNLWLELVPHLHNL----- 
-MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELG---------------------------------------------------------------------------------------AEEEMEAGRPRPVLRS-VNSREPSQVIFCNRSP----RVV--LPV------WLNFDGEPQPYPTLPPG-----------------T--GR-RI-H--SY--------RGHLWLFRD--AGTHDGLL-VNQTE----LFVPSLNVDGQPI-FANITLP-----------------------------------------------------------------------------------------VYTL---------------------------------------------------------------------------------------------KER-----------CL--QV-V-----R-------SLVKPENYRRLDIVRSLYEDLEDHP-NVQKDLER---LTQERI-----AH---------------------------------------QRMGD 
 
VPTPNQQPEVLYNQIFIN------NEWHDAVSKKTFPTVNPSTGDVICHVAEGDKADVDRAVKAARAAFQLG--SPWRRMDASERGRLLNRLADLIERDRT--------------YLAALETLDNGKPYIISYLVDLDMVLKCLRYYAGWADKYHGKTIPIDGDYFSYTRHEPVGVC-------G-Q-I---IPWNFPLLMQAWKLGPALATGNVVVMKVAEQTPLTALYVANLIKEAGFPPGVVNVIPGFGPTAGAAIASHEDVDKVAFTGSTE-VGHLIQVAAGKSNLKRV----------------TL-E---IG--GKSPNIIMSDADMDWAVEQAHFALFFNQGQCCCAGSRTFVQEDIYAEFVERSVARAKSRVVGNPFDSRTEQGPQVDETQFKKVLGYIKSGKEEGLKLLCGGGAAADRGYFIQPTVFGDLQDGMTIAKEEIFGPVMQILKFKSMEEVVGRANNSKYGLAAAVFTKDLDKANYLSQALQAGTVWVNCYDVFGAQSPFGGYKLSGSGRELGEYGLQAYT--EV-KTVTVRVPQKNS-- 
------------------MPRRAEN-----------------------------------------------WDEAE--VG---AEEA--GV---------EEYGPEEDGGEESGAE-ESG----------PE--ESGP--EELGAEEEM--EAGR-------------PRPVLRSVNSREPSQVIFCNRSPRVV-----LP--VW--LNFDGEPQPYPTLP----------------------------PGTGRR-IHS-YRGHLWLFRDAGT-HDG--LL-VNQ---TELFVPSLNVDGQPIFANITLPVYTLKERCLQVV-----------------------------RSLV-K----------------------------------------------------------------------------------------------------------------------PENYRRL----DI--VRS-LYEDLE------------DHP--------NVQ--KDLERLTQERIAHQRM--------GD 
 
(a)Residue pairs that are structurally aligned by TM-align program are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade.(more about the colors used)
(b)Ranking of proteins is based on TM-score of the structural alignment between Model1 and the PDB structures in our template library.
(c)RMSDa is the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.

  Function Prediction

  Predicted EC Numbers

RankTM-scoreRMSDaIDENaCov.EC-ScorePDB
Hit		EC No.
10.4096 5.80 0.09 0.700.45301wndA 1.2.1.19
 
20.4430 6.07 0.07 0.780.45192h7cD 3.1.1.1
 
30.3830 5.44 0.11 0.630.44791fpsA 2.5.1.12.5.1.10
 
40.4278 5.36 0.08 0.690.44151fgjA 1.7.3.4
 
50.3558 5.48 0.12 0.590.43972zatD 1.1.1.184
 
(a)Ranking is based on EC-score, which is a confidence score for the Enzyme Classification (EC) Number prediction.
(b)RMSDa is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)IDENa is percentage sequence identity in the structurally aligned region.
(d)Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)EC-Score is defined based on the C-score of the structure prediction and similarity of the model with known enzyme structures, as identified using both global and local structural alignment programs. The global similarity score uses TM-score, IDENa,RMSDa and Cov. of the structural alignment by TM-align, while the local match compares the structural and chemical similarity of local spatial motifs in the model with known catalytic site of enzymes. A prediction with a EC-score >1.1 signifies a prediction with high confidence (upto 3 digit numbers of EC) and vice versa (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).

  Predicted GO terms

RankTMscoreRMSDaIDENaCov.PDB
Hit		Fh-ScoreAssociated GO Terms
10.6365 1.28 1.00 0.671vcbL 2.44 GO:0008134 GO:0006950 GO:0007049 GO:0008283 GO:0009056 GO:0009058 GO:0009653 GO:0016043 GO:0016265 GO:0019222 GO:0043170 GO:0044237 GO:0044238 GO:0048518 GO:0048519 GO:0048856 GO:0048869 GO:0050793 GO:0050794 GO:0065007 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005783 GO:0005829 GO:0016020 GO:0043229
20.4126 1.26 1.00 0.431vcbL 1.58 GO:0008134 GO:0006950 GO:0007049 GO:0008283 GO:0009056 GO:0009058 GO:0009653 GO:0016043 GO:0016265 GO:0019222 GO:0043170 GO:0044237 GO:0044238 GO:0048518 GO:0048519 GO:0048856 GO:0048869 GO:0050793 GO:0050794 GO:0065007 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005783 GO:0005829 GO:0016020 GO:0043229
30.2272 1.04 0.98 0.231vcbL 0.85 GO:0008134 GO:0006950 GO:0007049 GO:0008283 GO:0009056 GO:0009058 GO:0009653 GO:0016043 GO:0016265 GO:0019222 GO:0043170 GO:0044237 GO:0044238 GO:0048518 GO:0048519 GO:0048856 GO:0048869 GO:0050793 GO:0050794 GO:0065007 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005783 GO:0005829 GO:0016020 GO:0043229
40.4177 5.68 0.10 0.711uxvA 0.46 GO:0008886 GO:0055114
50.4096 5.79 0.10 0.701wndD 0.45 GO:0016646 GO:0019145 GO:0051287 GO:0006598 GO:0009056 GO:0009308 GO:0009310 GO:0009447 GO:0009987 GO:0042219 GO:0042402 GO:0044248 GO:0055114
60.3651 5.73 0.12 0.621wmwD 0.45 GO:0006720 GO:0008299 GO:0009058 GO:0009987 GO:0044237
70.4125 5.63 0.09 0.701o9jD 0.44 GO:0004029 GO:0055114 GO:0005622 GO:0005623 GO:0005737
80.3458 5.56 0.13 0.581d4xA 0.43 GO:0005515 GO:0005524 GO:0030554 GO:0000003 GO:0000910 GO:0002119 GO:0009792 GO:0040007 GO:0040035 GO:0048513 GO:0048856 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0043229
90.3558 5.48 0.12 0.592zatD 0.43 GO:0004090 GO:0005488 GO:0055114 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005777 GO:0043229 GO:0044444
100.4148 5.59 0.09 0.703d64B 0.42 GO:0004013 GO:0005488 GO:0006730 GO:0009987 GO:0005622 GO:0005623 GO:0005737

Consensus Prediction of Gene Ontology terms 
Molecular Function  Biological Process Cellular Location
GO termGO-Score  GO termGO-Score GO termGO-Score
GO:00055150.529  GO:00488561.016  GO:00057370.783
GO:00081340.486  GO:00099870.661  GO:00056230.783
GO:00038240.262  GO:00442370.618  GO:00056220.783
GO:00164910.178  GO:00442380.576  GO:00432290.613
GO:00169030.135  GO:00090580.531  GO:00444440.570
GO:00166200.135  GO:00090560.531  GO:00057390.529
GO:00168010.084  GO:00488690.486  GO:00160200.486
GO:00167870.084  GO:00431700.486  GO:00057830.486
GO:00040130.084  GO:00507890.486  GO:00058290.486
GO:00168020.084  GO:00192220.486  GO:00056340.486

(a)Ranking in the first table is based on a function prediction score (Fh-score), which is calculated based on the C-score of the structure prediction and the TM-score, IDENa, RMSDa and Cov. of the structural alignment by TM-align between the predicted model and the PDB structures (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).
(b)RMSDa is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)IDENa is the percentage sequence identity in the structurally aligned region.
(d)Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)A consensus prediction of GO terms is derived from the structural analogs that have an Fh-score of >=1.0. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on the Fh-score of the template from which the GO term is derived. A prediction with a GO-score >0.5 signifies a prediction with high confidence and vice versa.


  Predicted Binding Site


Binding site residues in the model:
ARG:60  LEU:63  ARG:64  SER:65  PHE:76  PRO:86  TRP:88  
LEU:89  GLY:93  GLU:94  SER:111  TYR:112  GLY:114  HIS:115  
LEU:116  TRP:117  LEU:118  PHE:119  LEU:129  VAL:130  GLU:134  
LEU:135  PHE:136  VAL:137  PRO:138  ALA:149  VAL:194  ASP:197  
LEU:198  GLU:199  ARG:200  LEU:201  THR:202  
Identified analogs with similar binding site:

RankPDB
Hit		TM-scoreRMSDaIDENaCov.BS-scoreDownload
Complex		Binding site residues on the predicted model
11fgjA0.4278 5.36 0.08 0.70 0.74Download60,63,64,65,76,86,88,89,93,94,111,112,114,115,116,117,118,119,129,130,134,135,136,137,138,149,194,197,198,199,200,201,202
21pr9A0.3617 5.28 0.10 0.58 0.37Download38,40,42,82,83,85,100,121,122,123,124,125,126,164
31p33B0.3388 6.22 0.10 0.62 0.35Download42,82,83,85,100,120,121,122,123,124,125,126,127,156,160,163,164,167,188,191,192,193
41ys4A0.3695 6.04 0.07 0.67 0.34Download20,21,23,25,26,85,88,90,95,96,97,125,193,194
51xkqA0.3575 5.33 0.09 0.58 0.33Download40,42,82,85,120,121,122,123,124,125,126,127,164,167
62hy810.3478 6.28 0.08 0.67 0.33Download13,14,15,20,23,60,61,64,65,88,89,90,91,92,93,94,96
71p33B0.3388 6.22 0.10 0.62 0.28Download82,121,123,124,125,126,156,161,162,163,164,165,166,167,168,169,170,188
82d1yC0.3569 5.47 0.10 0.59 0.28Download82,83,84,85,100,101,102,103,120,121,122,123,124,125,126,127,128,153,156,160,161,162,163,164,165
91i10A0.3446 6.30 0.08 0.65 0.27Download82,83,84,85,98,99,100,101,107,119,120,121,122,123,124,125,126,127,128,164,192
102pd6D0.3568 5.48 0.08 0.59 0.26Download42,82,85,121,122,123,124,125,126,127,164,165,167

(a)Ranking of the analogs in the table is based on their BS-score. BS-score is calculated by first finding the structural analogs of the predicted model based on their TM-score, IDENa and Cov. of the structural alignment. The ligand(s) in the analog structure are then transferred onto the model and the fitness of the ligand-model complex (BS-score) is calculated by comparing the local structure and sequence similarity in the binding site region. (For detail, see Kucukural, Szilagyi, Ambrish, and Zhang, Template based ligand binding site prediction on modeled protein structure, 2009, in preparation).
(b) A BS-score of >0.5 signifies a binding site prediction with high confidence and vice-versa.
(c)RMSDa the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.
(f)The image shows the ligand-protein complex with the best BS-score. The ligand is depicted in magenta colored ball & stick, the predicted binding site residues interacting with the ligand are shown as transparent green spheres, while the N & C terminus in the model are marked by blue and red spheres respectively.



 Please cite following articles when you use the I-TASSER server:
1.Yang Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9:40 (2008).
2.Yang Zhang. Template-based modeling and free modeling by I-TASSER in CASP7. Proteins, 8: 108-117 (2007).
3.Sitao Wu, Jeffrey Skolnick, Yang Zhang. Ab initio modeling of small proteins by iterative TASSER simulations. BMC Biology, 5:17 (2007).