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I-TASSER results for job id S34213

  Submitted Sequence

>apoe4
KVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQEL
RALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVRGRLVQYRG
EVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRER
LGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVR
AKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH

  Predicted Secondary Structure

Sequence                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300
                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |
KVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVRGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH
PredictionCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
Conf.Score98788899999877777778999859999999999999998265989998985266503541268999999999999999850214767742427799999999999999999859999999999987699999998889999999998889999998155899999999963999999865599999999999999998766999999999999999999997188899888779999998899899999999999999998888999999999999999998773899889984659

  Top 5 Models predicted by I-TASSER

Download Model 1 Download Model 2 Download Model 3 Download Model 4 Download Model 5
C-score=-1.34
 C-score=-2.97
 C-score=-3.81
 C-score=-3.85
 C-score=-3.62
 
Estimated accuracy of Model1: 0.55±0.15 (TM-score)    9.2±4.6Å (RMSD)    (Read more about C-score of generated models)

  Top 10 templates used by I-TASSER

RankPDB
Hit		Iden1Iden2Cov.Norm.
Z-score		Download
Align.		                   20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280
                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   
Sec.Str
Seq		CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
KVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVRGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH
11ya9A 0.79 0.45 0.56 1.33Download -----------PEVTDQLEWQSNQPWEQALNRFWDYLRWVQTLSDQVQEELQSSQVTQELTALMEDTMTEVKAYKKELEEQLGPVAEETRARLGKEVQAAQARLGADMEDLRNRLGQYRNEVHTMLGQSTEEIRARLSTHLRKMRKRLMRDAEDLQKRLAVYKAG------AGVSAIRERLGPLV------------------------------------------------------------------------------------------------------------------
21nfnA 0.99 0.44 0.44 6.71Download ----------------------GQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQ----------RLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQA---------------------------------------------------------------------------------------------------------------------------------------
32a01A 0.16 0.19 0.81 3.52Download ------------------DEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGL------------------LPVLESFKVSFLSALEEYTKKLNTQ--------------------
41av1A 0.18 0.17 0.67 2.07Download MLKLL------------------------------------------------------------DNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEK------------------AKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNT--------------------Q
52a01A 0.16 0.19 0.81 3.46Download ------------------DEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQ--------------------------------------
61ya9A 0.79 0.45 0.56 2.09Download -----------PEVTDQLEWQSNQPWEQALNRFWDYLRWVQTLSDQVQEELQSSQVTQELTALMEDTMTEVKAYKKELEEQLGPVAEETRARLGKEVQAAQARLGADMEDLRNRLGQYRNEVHTMLGQSTEEIRARLSTHLRKMRKRLMRDAEDLQKRLAVYK------AGAGVSAIRERLGPLV------------------------------------------------------------------------------------------------------------------
72a01A 0.17 0.19 0.81 3.06Download ------------------DEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALED------------------LRQGLLPVLESFKVSFLSALEEYTKKLNTQ--------------------
81nfnA 0.99 0.44 0.44 5.18Download ----------------------GQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQ----------RLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQA---------------------------------------------------------------------------------------------------------------------------------------
91av1A 0.18 0.17 0.67 1.19Download ------------------------------------------------------------MLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLP------------------VLESFKVSFLSALEEYTKKLNTQ--------------------
101nfnA 0.99 0.44 0.44 4.22Download ----------------------GQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQ----------RLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQA---------------------------------------------------------------------------------------------------------------------------------------
(a)All the residues are colored in black; however, those residues in template which are identical to the residue in the query sequence are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade. (more about the colors used)
(b)Rank of templates represents the top ten threading templates used by I-TASSER.
(c)Ident1 is the percentage sequence identity of the templates in the threading aligned region with the query sequence.
(d)Ident2 is the percentage sequence identity of the whole template chains with query sequence.
(e)Cov. represents the coverage of the threading alignment and is equal to the number of aligned residues divided by the length of query protein.
(f)Norm. Z-score is the normalized Z-score of the threading alignments. Alignment with a Normalized Z-score >1 mean a good alignment and vice versa.
(g)Download Align. provides the 3D structure of the aligned regions of the threading templates.
(h)The top 10 alignments reported above (in order of their ranking) are from the following threading programs:
       1: MUSTER   2: HHSEARCH   3: SP3   4: PROSPECT2   5: FUGUE   6: PPA-I   7: SPARKS   8: HHSEARCH I   9: MUSTER   10: HHSEARCH II   

  10 proteins in PDB which are structurally closest to the first I-TASSER model (identified by TM-align)

RankTM-scoreRMSDaIDENaCov.PDB
Hit
10.4306 5.31 0.07 0.641st6A
Model1
 
20.4285 5.81 0.02 0.671jb0A
Model1
 
30.4259 4.47 0.10 0.582ixrA
Model1
 
40.4221 6.16 0.05 0.692pmzB
Model1
 
50.4179 5.63 0.06 0.642j91A
Model1
 
60.4169 5.16 0.04 0.603c8tA
Model1
 
70.4157 5.60 0.07 0.641w27A
Model1
 
80.4152 5.26 0.07 0.611re5A
Model1
 
90.4148 5.32 0.05 0.611c3cA
Model1
 
100.4143 6.20 0.04 0.691fohA
Model1
 
Structural alignment using TM-align
 
VPRMPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVSAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVRAAQMLQADPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGI-LEYLTV-AEVVETMEDLVTYTKNLGPG----MTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNTKSQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALALIDSKMNQAKGWLRDPNAPPGDAGEQAIRQILDEAGK--A-------------------------------------------GELCAGKERREILGTCKTLGQMTDQLADL-RA-RGQGATPMAMQKAQQVSQGLDLLTAKVENAARKLEAMTNSKQAIAKKIDAAQNWLADPNGGSEGEEHIRGIMSEARKVAELCEEPKERDDILRS----------------------------------LGEISALTAKLSDLRRHGKGDSPEARALAKQIATSLQNLQSKTNRAVANTRPVKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGRRLANVMMGPYRQDLLAKCDRVDQLAAQLADLAARGEGESPQARAIAAQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPSDTPNREEVFEERAANFENHAARLGATAEKAAAVGTANKTTVEGIQATVKSARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANMQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEHLHLTPPPPEEKDEEFPEQKAGEAINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWY-------------------- 
------------------------------------------------------------------------------------------------------------------------------------------------KVE-QA-VE-------------------TEPEPE--LR-QQ--TEWQ----S---G------------------------------------------------QRWEL-AL---------G--RF-WDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVRGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQ-KRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGE--------RLRARMEEMGSRTR-----------------DRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDM--Q---------RQWAGL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VEKVQAAVGTSAAPVPSDNH 
 
RVVVDNDPVPTSFEKWAKPGHFDRTLARGPQTTTWIWNLHALAHDFDTHTSDLEDISRKIFSAHFGHLAVVFIWLSGMYFHGAKFSNYEAWLADPTGIKPSAQVVWPIVGQGILNGDVGGGFHGIQITSGLFQLWRASGITNEFQLYCTAIGGLVMAGLMLFAGWFHYHKRAPKLEWFQNVESMLNHHLAGLLGLGSLAWAGHQIHVSLPINKLLDAGVAAKDIPLPHEFILNPSLMAELYPKVDWGFFSPFFTFNWAAYSDFLTFNGGLNPVTGGLWLSDTAHHHLAIAVLFIIAGHMYRTNWGIGHSLKEILEAHKGPFTGAGHKGLYEVLTTSWHAQLAINLAMMGSLSIIVAQHMYAMPPYPYLATDYPTQLSLFTHHMWIGGFLVVGGAAHGAIFMV-RDYDPAMNQNNVLDRVLRH-RDAIISH--LNWVCIFLGFHSFGLYVHNDTMRAFGRPQDMFSDTGIQLQPV-----------FAQWVQNLHTLAPGGTAPNAAATASVAFGGD---VVAVGGKVAMM-------PIV-LGTADFMVHHIHAFTIHVTVLILLKGVLFARSSRLIP-----------DKANLGFRFPCDGPGRGGTCQVSGWDHVFLGLFWMYNCISVVIFHFSWKMQSDVWGTVAPDGTVSHITGGNFAQSAITINGWLRDFLW--AQASQVIGSYGSALSAYGLLFLGAHFIWAFSLMFLFSGRGYWQELIESIVWAHNKLKVAPAIQPRALSIIQGRAVGVAHYLLGGIATTWAFFLARIISVG-------------------------------------------------------------- 
------------------------------------------------------------------------------------------------------------------------------KVEQAVETEPEP-----------------------------------------------------------------E----L------------------------------------------------------RQQTEWQSG--QR----------------------------------------------------------------------------------WEL-A-----------L--GRFWDYLRWVQTLSEQVQEELLSSQVTQELRALM----DE-TMK-EL--K---AYK-----------------------------------------SELEEQLTPVAEETRARL-SK---------------E-LQAAQARLGA--------DMEDVRGRLVQYRGEVQAMLGQSTEELRVRLASHLRK-LRK-RLLRDADDLQKRLAVYQAGAREGA-E------------RGLSAIRERLGPLVEQGRVRAATVGSLAGQPL-QERAQ----AWGER--------------------LRAR-MEEMGS-------RTRDRLDEVKEQVA----------------------------------------------------------------------EVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH 
 
A-M--TD---D---------------DLRAAGV--DRRVPEQKLGAAID-EFASLRLPDRIDGRFVDGR--RA--NL-TVF-DDARVAVRGHARAQRNLLERLET-EL----LQP--DPILQGLVDVIGQGKSDIDAYATIVEGLTKYFQSVADVMSKLQDYISAKDDKNMKIDGGKIKALIQQVIDHLPTMQLPKGADIARWRKELGDAVSISDSGVVTINPDKLIKMRDSLPP-DGTVWDTARYQAWNTAFSGQKDNIQ-NDVQTLVEKYSHQNSNFDNLVKVLSGAISTLTD---------------------------------------------------------------------------------------- 
-KVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQ------ELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGA--DMEDVRGRLVQYRGEVQAMLGQSTEELRVRL-A--------------------------------------------------------------S---------HLRKLRKRLLRDADDLQKRLAV---YQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH 
 
LTIDERWRVIEAYFKSKGLVRQHLDSYNDFVRNKLQEIIDEQGEIPTEIPGLKVRLGKIRIGKPRVRESDRGEREISPMEARLRNLTYAAPLWLTMIPVENNIEAEPEEVYIGDLPIMLKSAIDPISQYTLDKLIEIGEDPKDPGGYFIVNGSERVIVTQEDLAPNR-------------------------------------------------------------------------------------------VLV--DTGKTGSNITH-----------------TAKIISSTAGYRVPVTIERLKDGTFHVSFPAVPGKIPFVILMRALGILTDRDIVYAVSLDPEVQNELFPSLEQASSIANVDDALDFIGSRVA-IGQ----------------------------K--RE-NRIEKAQQIIDKYFLPHLGTSAEDRKKKAYYLAYAISKVIELYLGRREPD-----DKDHYANKRLRLAGDLFASLFRVAFKAFVKDLTYQLEKSKVRGRKL--ALK--ALVRPDIVTERIRHALATGNWVGGRTGVSQLLDRTNWLSMLSHLRRVISSLARGQPNFEARDLHGTQWGRMCPFETPEGPNSGLVKNLALMAQIAVGINERIVEKTLYEMGVVPVEEVILKWSKVILNGRLIGYYQDGGELANKIRERRRKGEISDEVNVGHIVTDFINEVHVNCDSGRVRRPLIIVSNGNPLVTIEDIEKLESGAITFDDLVRQGKIEYLDAEEEENAYVALEPNDLTPDHTHLEIWSPAILGITASIIPYPEHNQSPRNTYQSAMAKQALGLYAANYQLRTDTRAHLLHYPQRPLVQTRALDIIGYTNRPAGNNAILAVMSFTGYNMEDSIIMNRSSVERGMYRSTFFRLYSTEEVKYPGGQEDKIVMPEAGVRGYKGKEYYRLLEDNGVVSPEVEVKGGDVLIGKVSPPRAKRDTSIVTRHGEMGIVDLVLITETAEGNKLVKVRVRDLRIPTIGDKFASRHGQKGVIGMLIPQVDMPYTVKGVVPDIILNPHALPSRMTLGQIMEGIAGKYAALSGNIVDATPFYKTPIEQLQNEILRYGYLPDATEVVYDGRTGQKIKSRIYFGVVYYQKLHHMVADKLHARARGPVQILTRQPTEGRAREGGLRFGEMERDCLIGFGTAMLLKDRLLDNSDRTMIYVCDQCGYIGWYDKNKNKYVCPIHGDKSNLFPVTVSYAFKLLIQELMSMIISPRLVLEDKV 
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------KVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQ---------AAQARLGADMEDVRGRLVQYRG-----------EVQA------MLGQ-STEEL-------------------------------------------------RVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGS-------------------------------LAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLE----EQA--QQIRLQAEAFQARLKSWFE-PL-------VEDMQ-------R--QWAG----------------------------------------LVE-------------------------------------------------------------------------------------------------------------------------------------------------------------------KVQAAV-G-TSAAP----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------V-------------------------------------PSDNH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 
 
GDHGSPDSYR--SPLASRY------------------------ASPEMCFVFSDRYKFR-TWRQLWLWLAEAEQTLGLPITDEQIREMKSNLENIDFKMAAEEEKRLRHDVMAHVHTFGHCCPKAAGIIHLGATSCYVGDNTDLIILRNALDLLLPKLARVISRLADFAKERAS--LPTLGFTHFQPAQLTTVGKRCCLWIQDLCMDLQNLKRVRDD-LR-FRGVKGTTGTQASFLQLFEGDDHKVEQLDKMVTEKAGFKRAFI-------------ITGQTYTRKVDIEVLSVLASLGASVHKICTDIRLLANLKEMEEPFYKRNPMRSERC---------CSLA---RHLM---TLV--MDPLQTASVQWFERTLDDSANRRICLAEAFLTADTILNTLQNISEGLVVYPKVIERRIRQELP----------------------------------------------FMATENIIMAMVGGSRQDCHEKIRVLSQQAASVVKQEGGDNDLIERIQVDAYFSPIHSQLDHLLDPSSFTGRASQQVQRFLEEEVYPLLKPYES 
----------KVEQAVETEPEPELRQQTEWQSGQRWELALGRFWD-YLRWVQTLSEQV-QEEL---------------------------------------------LSSQV-TQ---------ELRALMDE--TMKELKAYKSELEEQLTPVAEETRARLSKELQAAQAR--LGADM-----------EDVRGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLR-KRLLRDADDLQKR-----------------------LAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAW-----------------GERLRARMEE---MGSR---TRDR--LDE--------------------------------------------------------------VKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGL---------------------------VEKVQA---AVGTSAAPVPSDNH------------------------------------------ 
 
PL--YG-RS-------------------------FAD--DKMRELFSAQSFISRCVETEVALARAQARLGIIPEDAAAGITAAARTFAPEMERLRDDTEIVGYPILPLVEQLSAHAGEAGKYLHWGATTQDIMDTATVLQIRDGLALISRRIESVRKALAALARNHRD--TPMAGRTHLQHALPVTFGYKAAVWLSAFDRHAARLEEISPRVLV--V--EFSGASGTLASLGTRGLDVQRELARELNLGVPSIT------------------WHSARDAVAETVQFLALVSGSLGKLAMDISIMMTTELGEVAEPNPVSCELI---------LAGA---RIVR---NHATSMLDAMIHDFERATGPWHLEWSAVPEGFAVASGILYQAEFMLGGLQVFPDRMRENLDHSRG-------------------------------------------------LIVAEAVMMALAPHTGRKEAHDIV-YLGCRRAVEDKTGLFEVLRTMPEVAKPLGEEALRDLTDPRNYLGSAGAMVDNVLGGR 
--KVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEE--L-----------------------------------------------LSSQVTQE--L--------RALM-D---ET-MKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADM---------EDV-RGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKR--LLRDADDLQ--------------------KRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQ-AW--------------GERLRARMEE---MGSR---TRDR----------------------------------------------------------------LDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGL----------------------VE--KVQAA--VGTSAAPVPSDN---H------------------------------- 
 
EDPLYWGIAAEAMTGSH-------------------------------LDE--VKK-MVAE-YRKPVVKLGGETLTISQVAAISARDGSGVTVELSEAARAGVKASSDWVMDSMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGNGSDNTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKFLNQNITPCLPLRGTITDLVPLSYIAGLLTGRPNSKAVGPTGVILSPEEAFKLAGVEGGFFELQPKEGLALVNG--TAVGSGM--ASMV--LFE-ANILA-VLAEVMSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHILDGSAYVKAAQK--LHEMDPLQKPKQDR-YALRTSPQWLGPQIEVIRSSTKMIEREIN-SVNDNPLIDVSR----NKAIH-GGN-FQ--GTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNGL---PSNLSGGRNPSLDYGFKGAEIAMASYC-SELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTSEAVEILKLMSTTFLVGLCQAIDLRHLEENLKSTVKNTVSSVAKRVLTMGVNGELHPSRFCEKDLLRVVDREYIF-----------------------------------------AYIDDPCSATYPLMQKLRQTLVEHALKNGDNERNLSTSIFQKIATFEDELKALLPKEVESARAALESGNPAIPNRIEECRSYPLYKFVRKELGTEYLTGEKVTSPGEEFEKVFIAMSKGEIIDPLLECLESWNGAPLPIC- 
-----------------KVEQAVETEPEPELRQQTEWQSGQRWELALGR--FWDY-LRWV-QT------------LSEQVQ-EE------------------------------------------------------------------------------------LLSSQVT-QELRAL---------------------------------------------------------------------------------------MDET-----MKEL--KAYK-SELE--EQ-------------------------------L-TPVAEETRARLSKELQAAQAR-LGADMEDVRGR-LVQYRGEVQAMLGQSTEELRVRLASHLRKLRK--RLLRDAD------DLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPL-QERAQAWGERLRA-------RMEEMGSRTRDRLD-EVK------------------------------------------------------------------------------------------------------------EQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQW--A--GLVEKVQAAVG--TSAAPVP----SD------N----------------------------------------------------------------------------------------------------H 
 
NQ--LF-DA-Y--------------------FT-APAMREIFSDRGRLQGMLDFEAALARAEASAGLVPHSAVAAIEAACQAERYDTGALANAIATAGNSAIPLVKALGKVIATGVPEAERYVHLGATSQDAMDTGLVLQLRDALDLIEADLGKLADTLSQQALKHADTPLVGRTWLQHATPVTLGMKLAGVLGALTRHRQRLQELRPRLLV-LQFGGA-SGSLAALGSKAMPVAEALAEQLKLTLPEQP------------------WHTQRDRLVEFASVLGLVAGSLGKFGRDISLLMQTEAGEVFEPSAPKRNPVGAAVL---------IGA--ATRV-PGLL--STLFAAMPQEHERSLGLWHAEWETLPDICCLVSGALRQAQVIAEGMEVDAARMRR-----------------------------------------------NL-DLTQGLVLAEAVSIVLAQRLGRDRAHHLLEQCCQRAVAEQRHLRAVLGDEPQVSAELSGEELDRLLDPAHYLGQARVWVARAVSEHQRFTA 
--KVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQT-LSEQVQ--------------------------------------E---------ELLSSQVT-Q----------ELRALMDET--MKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADM-----------EDVRGRLVQYRGEVQAMLGQSTEELRVRLASHLRKL-RKRLLRDADDLQ-------------------KRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQ--AW------------------GERLRARMEE--MGSRT-RDR--LDEV-----------------------------------------------------KEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLV-EKVQAAV----------------------------------GTSAAPVPSDNH--------------------------------------- 
 
--V--ER-------------------------YSLS-PMKDLWTEEAKYRRWLEVELAVTRAYEELGMIPKGVTERIRNNAKIDVELFKKIEEKTNHDVVAFVEGIGSMIGEDSRFFHYGLTSSDVLDTANSLALVEAGKILLESLKEFCDVLWEVANRYKH--TPTIGRTHGVHAEPTSFGLKVLGWYSEMKRNVQRLERAIEEVSY--G--KISGAVGNYANVPPEVEEKALSYLGLKPEPV-----------------STQVVPRDRHAFYLSTLAIVAAGIERIAVEIRHLQRTEVLEVEEPFRKSAMPHKKNPITCERL---------TGLS---RMMR---AYV--DPSLENIALWHERDISHSSVERYVFPDATQTLYYMIVTATNVVRNMKVNEERMKKNID-----------------------------------------------LTKGLVFSQRVLLKLIEKGLTRKEAYDIVQRNALKTWNSEKHFLEYLLEDEEVKKLVTKEELEELFDISYYLKHVDHIFERFEK 
KVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSE-QVQEEL--------------------------------------------LSSQVTQE---------L-RALM-D---ET-MKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADM---------EDV-RGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKR--LLRDADDL-----------------QKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQ-AW------------------------GERLRARMEE---MGSR---TRDR--LDE---------------------------------------------------------VKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVE-KV--------------------------Q--AA--VGTSAAPVPSDN--H------------------------------ 
 
TKYSESYCDVLIVGAGPAGLMAARVLSE--------------------YVRQKPDLK-VRIIDKRSTKVYNGQADGLQCRTLESLKNLGLADKILSE-ANDMSTIALYNPDENGHIRRTDRIPDTLPGISRYHQVVLHQGRIERHILDSIAEISDTRIKVERPLIPEKMEIDSSKAEDPEAYPVTMTLRYMSDHESTPLQFGHKTENSLFHSNLQTQEEEDANYRLPEGKEAGEIETVHCKYVIGCDGGHSWVRRTLGFEMIGEQTDYIWGVLDAVPASNFP-DIRSRCA-IHSAESGSIMIIPRENNLVRFYVQLQFTPEVVIANAKKIFHPYTFDVQQLDWFTAYHIGQRVTEKFSKDERVFIAGDACHTHSPKAGQGMNTSMMDTYNL-GWKLGLVLT-------------G--RAK--RDILKT-YEEERHAFAQALIDFDHQFSRLFSGRPAKDVADEM----GVSMDVFKEAFVKGNEFAS-GTAINYDENLVTDKKSSKQELAKNCVVGTRFKSQPVVRHSEGLWMHFGDRLVTDGRFRIIVFAGKATDATQMSRIKKFSAYLDSENSVISLYTPKVSDRNSRIDVITIHSCHRDDIEMHDFPAPALHPKWQYDFIYADCDSWHHPHPKSYQAW------------------GV-DETKGAVVVVRPDGYTSLVTDLEGTAEIDRYFSGILVEPKEKS------GAQ-TEADWTKS------------------- 
----------------------------KVEQAVETEPEPELRQQTEWQSGQRW---EL------------------------ALGRFWDYL-RW--VQTL--------------------------------SEQV-------------------------------------------------------------------------------------------------------------------------------------------------QEELLSSQVTQEL-RALM-------------DE--------------------------------TMKE---------------------LKA--YKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVRGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRL-AVYQAGAREGAERGLSAIRERLGPLVEQG-----------------------RVRA---------------A--TVGSLAGQ-----------------------------PLQ-ERAQAWGER---------------------LRARMEE----MGSRTRD------RLDEVKE-QVAEVRAKLEEQAQQIRLQAEAFQARLK------------SWFEPL------------------VEDMQRQWAGLV------EKVQAAVGTSAAPVPSDNH 
 
(a)Residue pairs that are structurally aligned by TM-align program are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade.(more about the colors used)
(b)Ranking of proteins is based on TM-score of the structural alignment between Model1 and the PDB structures in our template library.
(c)RMSDa is the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.

  Function Prediction

  Predicted EC Numbers

RankTM-scoreRMSDaIDENaCov.EC-ScorePDB
Hit		EC No.
10.3725 3.15 0.13 0.450.41101pv3A 2.7.10.2
 
20.4157 5.60 0.07 0.640.38941w27A 4.3.1.24
 
30.4159 6.17 0.07 0.680.38681pn0C 1.14.13.7
 
40.3920 6.28 0.07 0.670.37953czoD 4.3.1.3
 
50.3873 6.26 0.07 0.630.37941y79A 3.4.15.5
 
(a)Ranking is based on EC-score, which is a confidence score for the Enzyme Classification (EC) Number prediction.
(b)RMSDa is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)IDENa is percentage sequence identity in the structurally aligned region.
(d)Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)EC-Score is defined based on the C-score of the structure prediction and similarity of the model with known enzyme structures, as identified using both global and local structural alignment programs. The global similarity score uses TM-score, IDENa,RMSDa and Cov. of the structural alignment by TM-align, while the local match compares the structural and chemical similarity of local spatial motifs in the model with known catalytic site of enzymes. A prediction with a EC-score >1.1 signifies a prediction with high confidence (upto 3 digit numbers of EC) and vice versa (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).

  Predicted GO terms

RankTMscoreRMSDaIDENaCov.PDB
Hit		Fh-ScoreAssociated GO Terms
10.7885 1.14 0.17 0.812a01C 1.01 GO:0001540 GO:0005543 GO:0015485 GO:0017127 GO:0019899 GO:0034191 GO:0042802 GO:0060228 GO:0002376 GO:0009987 GO:0032501 GO:0044237 GO:0050789 GO:0050896 GO:0051179 GO:0065007 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005788 GO:0030139 GO:0031410 GO:0031974 GO:0034364 GO:0043229 GO:0043231 GO:0044444 GO:0044446
20.4219 5.88 0.11 0.652r0pA 0.49 GO:0004497 GO:0008152
30.3760 5.48 0.12 0.562p7nA 0.44 GO:0009405
40.3670 3.72 0.14 0.462p7vA 0.43 GO:0030528 GO:0006139 GO:0009058 GO:0009889 GO:0009987 GO:0043283 GO:0044249 GO:0044260 GO:0050794 GO:0060255 GO:0065007 GO:0005622 GO:0005623 GO:0005737
50.4278 4.83 0.08 0.612d4yB 0.43 GO:0003774 GO:0005198 GO:0001539 GO:0006928 GO:0009296 GO:0010926 GO:0043064 GO:0044085 GO:0005576 GO:0005623 GO:0009288 GO:0009424
60.3779 5.34 0.11 0.561ij6A 0.43 GO:0005509 GO:0043169
70.3920 4.94 0.10 0.552zbiB 0.43 GO:0005198 GO:0001539 GO:0006928 GO:0043064 GO:0005623 GO:0009288 GO:0009420
80.4014 5.09 0.09 0.592d4yB 0.42 GO:0003774 GO:0005198 GO:0001539 GO:0006928 GO:0009296 GO:0010926 GO:0043064 GO:0044085 GO:0005576 GO:0005623 GO:0009288 GO:0009424
90.4211 4.22 0.09 0.561l7cC 0.42 GO:0005198 GO:0017166 GO:0045296 GO:0051015 GO:0008283 GO:0009653 GO:0010926 GO:0022610 GO:0032501 GO:0048869 GO:0050789 GO:0065007 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005912 GO:0005915 GO:0015629 GO:0016327 GO:0030027 GO:0031252 GO:0043229 GO:0043296
100.3768 4.54 0.11 0.492z0vA 0.41 GO:0008289 GO:0042802 GO:0006897 GO:0007154 GO:0007165 GO:0007417 GO:0010324 GO:0048856 GO:0065007 GO:0005622 GO:0005623 GO:0005737 GO:0005769 GO:0016020 GO:0031901 GO:0043229 GO:0044440 GO:0044444

Consensus Prediction of Gene Ontology terms 
Molecular Function  Biological Process Cellular Location
GO termGO-Score  GO termGO-Score GO termGO-Score
GO:00054880.266  GO:00099870.395  GO:00056230.473
GO:00055150.224  GO:00511790.310  GO:00444640.473
GO:00428020.182  GO:00442370.285  GO:00057370.306
GO:00082890.182  GO:00507890.267  GO:00056220.306
GO:00051980.169  GO:00650070.267  GO:00444240.306
GO:00053190.141  GO:00081520.232  GO:00055760.226
GO:00228920.141  GO:00325010.224  GO:00432290.224
GO:00152480.141  GO:00488700.207  GO:00432260.224
GO:00052150.141  GO:00160430.169  GO:00160200.201
GO:00015400.141  GO:00109260.167  GO:00432270.182

(a)Ranking in the first table is based on a function prediction score (Fh-score), which is calculated based on the C-score of the structure prediction and the TM-score, IDENa, RMSDa and Cov. of the structural alignment by TM-align between the predicted model and the PDB structures (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).
(b)RMSDa is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)IDENa is the percentage sequence identity in the structurally aligned region.
(d)Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)A consensus prediction of GO terms is derived from the structural analogs that have an Fh-score of >=1.0. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on the Fh-score of the template from which the GO term is derived. A prediction with a GO-score >0.5 signifies a prediction with high confidence and vice versa.


  Predicted Binding Site


Binding site residues in the model:
TRP:39  LEU:43  SER:44  GLN:46  VAL:47  GLN:48  LEU:51  
GLU:70  ALA:73  TYR:74  LYS:75  SER:76  GLU:77  LEU:78  
GLU:80  GLN:81  LEU:137  ILE:177  LEU:181  
Identified analogs with similar binding site:

RankPDB
Hit		TM-scoreRMSDaIDENaCov.BS-scoreDownload
Complex		Binding site residues on the predicted model
11jb0A0.4285 5.81 0.02 0.67 0.91Download39,43,44,46,47,48,51,70,73,74,75,76,77,78,80,81,137,177,181
23bz1C0.3508 6.30 0.06 0.58 0.81Download10,21,22,23,24,25,26,27,28,29,30,31,32,34,35,104
32vf8B0.3278 6.10 0.07 0.53 0.79Download110,114,117,198,199,201,202,203,204,205,206,207,208,209,210,211,212,213,276
43bz1C0.3508 6.30 0.06 0.58 0.53Download40,43,44,47,67,70,71,74,78,129,130,131,132,133,134,135,136,137,138,139,140,141,144,173,175,176,177,178,179,180,181,182,183,184,185,186,187,188
51bpwA0.3529 6.30 0.05 0.57 0.42Download220,221,222,223,224,225,226,227,228,260,261,264
62d4eC0.3528 6.33 0.06 0.58 0.41Download198,213,216,217,218,219,220,221,222,223,264,268
73fg0C0.3591 6.29 0.06 0.58 0.35Download198,213,216,217,218,219,220,221,222,223,264,265,268,269
82jg7A0.3483 6.41 0.06 0.57 0.34Download198,213,216,217,218,219,220,221,222,223,264,268
91jehA0.3322 6.28 0.06 0.56 0.34Download1,2,3,6,26,27,28,29,30,31,32,33,34,36,37,38,40,41,118,185,189,196,200,204
102o2qA0.3583 6.47 0.05 0.60 0.33Download198,202,213,214,215,216,217,218,219,220,221,222,223,224,260,261,264,265,268

(a)Ranking of the analogs in the table is based on their BS-score. BS-score is calculated by first finding the structural analogs of the predicted model based on their TM-score, IDENa and Cov. of the structural alignment. The ligand(s) in the analog structure are then transferred onto the model and the fitness of the ligand-model complex (BS-score) is calculated by comparing the local structure and sequence similarity in the binding site region. (For detail, see Kucukural, Szilagyi, Ambrish, and Zhang, Template based ligand binding site prediction on modeled protein structure, 2009, in preparation).
(b) A BS-score of >0.5 signifies a binding site prediction with high confidence and vice-versa.
(c)RMSDa the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.
(f)The image shows the ligand-protein complex with the best BS-score. The ligand is depicted in magenta colored ball & stick, the predicted binding site residues interacting with the ligand are shown as transparent green spheres, while the N & C terminus in the model are marked by blue and red spheres respectively.



 Please cite following articles when you use the I-TASSER server:
1.Yang Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9:40 (2008).
2.Yang Zhang. Template-based modeling and free modeling by I-TASSER in CASP7. Proteins, 8: 108-117 (2007).
3.Sitao Wu, Jeffrey Skolnick, Yang Zhang. Ab initio modeling of small proteins by iterative TASSER simulations. BMC Biology, 5:17 (2007).