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I-TASSER results for job id S34335

Submitted Sequence

>your_protein
MARELRALLLWGRRLRPLLRAPALAAVPGGKPILCPRRTTAQLGPRRNPAWSLQAGRLFS
TQTAEDKEEPLHSIISSTESVQGSTSKHEFQAETKKLLDIVARSLYSEKEVFIRELISNA
SDALEKLHHKLVSDGQALPEMEIHLQTNAEKGTITIQDTGIGMTQEELVSNLGTIARSGS
KAFLDALQNQAEASSKIIGQFGVGFYSAFMVADRVEVYSRSAAPGSLGYQWLSDGSGVFE
IAEASGVRTGTKIIIHLKSDCKEFSSEARVRDVVTKYSNFVSFPLYLNGRRMNTLQAIWM
MDPKDVREWQHEEFYRYVAQAHDKPRYTLHYKTDAPLNIRSIFYVPDMKPSMFDVSRELG
SSVALYSRKVLIQTKATDILPKWLRFIRGVVDSEDIPLNLSRELLQESALIRKLRDVLQQ
RLIKFFIDQSKKDAEKYAKFFEDYGLFMREGIVTATEQEVKEDIAKLLRYESSALPSGQL
TSLSEYASRMRAGTRNIYYLCAPNRHLAEHSPYYEAMKKKDTEVLFCFEQFDELTLLHLR
EFDKKKLISVETDIVVDHYKEEKFEDRSPAAECLSEKETEELMAWMRNVLGSRVTNVKVT
LRLDTHPAMVTVLEMGAARHFLRMQQLAKTQEERAQLLQPTLEINPRHALIKKLNQLRAS
EPGLAQLLVDQIYENAMIAAGLVDDPRAMVGRLNELLVKALERH

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 620 640 660 680 700 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MARELRALLLWGRRLRPLLRAPALAAVPGGKPILCPRRTTAQLGPRRNPAWSLQAGRLFSTQTAEDKEEPLHSIISSTESVQGSTSKHEFQAETKKLLDIVARSLYSEKEVFIRELISNASDALEKLHHKLVSDGQALPEMEIHLQTNAEKGTITIQDTGIGMTQEELVSNLGTIARSGSKAFLDALQNQAEASSKIIGQFGVGFYSAFMVADRVEVYSRSAAPGSLGYQWLSDGSGVFEIAEASGVRTGTKIIIHLKSDCKEFSSEARVRDVVTKYSNFVSFPLYLNGRRMNTLQAIWMMDPKDVREWQHEEFYRYVAQAHDKPRYTLHYKTDAPLNIRSIFYVPDMKPSMFDVSRELGSSVALYSRKVLIQTKATDILPKWLRFIRGVVDSEDIPLNLSRELLQESALIRKLRDVLQQRLIKFFIDQSKKDAEKYAKFFEDYGLFMREGIVTATEQEVKEDIAKLLRYESSALPSGQLTSLSEYASRMRAGTRNIYYLCAPNRHLAEHSPYYEAMKKKDTEVLFCFEQFDELTLLHLREFDKKKLISVETDIVVDHYKEEKFEDRSPAAECLSEKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAKTQEERAQLLQPTLEINPRHALIKKLNQLRASEPGLAQLLVDQIYENAMIAAGLVDDPRAMVGRLNELLVKALERH
Prediction	CCHHHHHHHHHHHHCHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHCHHHCCCCCCCCCSSSSSSSSHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCSSSSSSCCCCCSSSSSSCCCCCCHHHHHHHHHHHSSCCHHHHHHHHHCCCCCCCCCCCCCCCCCSSSSSSCCSSSSSSCCCCCCCCSSSSSSCCCCSSSSSCCCCCCCCCSSSSSSCCCCHHHCCHHHHHHHHHHHHHHCCCCCSCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCSSSSSCCCCCSSSSSSSSSCCCCHHHHHHHHHCCCCSSSSSCCSSSCCCCCHHCHHHHHHSSSSSSCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHSSCCCCCCCCCCSSSHHHHHHCCCCCCCCSSSSSCCCHHHHHHCHCHHHHHHCCCSSSSSCCHHHHHHHHHHHHHCCCCCSSSCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCSSSSSSCCCCCCCCSSCCCCCHHHHHHHHHHHHHHCCCCCCCCCCSSSSCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
Conf.Score	90788999999852037660767640458896689986512038887863213555568843101222260101211013577752588774387899999874048825889999870899999999986048322467039997417888899996887878999998655455303799998742545445560042450003799950589999789998772799996799628971578777673489998676222245999999853176517876210230034566754367664887776778887655137980898512688624789998547682432124550377588878689768700228676622567886698986544887734399999999999999999999998799999999999989998761203345558998644251466877652456899986267878757999779899998493046787569579995565899999789863798715851222223232221023344555652877789999999973554336887754788874053375435677899999987502233567837998899989999999875388899999999999999867997079999999999999986239

Top 5 Models predicted by I-TASSER


Download Model 1	Download Model 2	Download Model 3	Download Model 4	Download Model 5
C-score=-0.92	C-score=-1.46	C-score=-1.79	C-score=-2.39	C-score=-2.56

Estimated accuracy of Model1: 0.60±0.14 (TM-score) 10.3±4.6Å (RMSD) (Read more about C-score of generated models)

Top 10 templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov.

Norm.
Z-score

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560                 580                 600                 620                 640                 660                 680                 700
                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |    

Sec.Str
Seq

CCHHHHHHHHHHHHCHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHCHHHCCCCCCCCCSSSSSSSSHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCSSSSSSCCCCCSSSSSSCCCCCCHHHHHHHHHHHSSCCHHHHHHHHHCCCCCCCCCCCCCCCCCSSSSSSCCSSSSSSCCCCCCCCSSSSSSCCCCSSSSSCCCCCCCCCSSSSSSCCCCHHHCCHHHHHHHHHHHHHHCCCCCSCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCSSSSSCCCCCSSSSSSSSSCCCCHHHHHHHHHCCCCSSSSSCCSSSCCCCCHHCHHHHHHSSSSSSCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHSSCCCCCCCCCCSSSHHHHHHCCCCCCCCSSSSSCCCHHHHHHCHCHHHHHHCCCSSSSSCCHHHHHHHHHHHHHCCCCCSSSCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCSSSSSSCCCCCCCCSSCCCCCHHHHHHHHHHHHHHCCCCCCCCCCSSSSCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
MARELRALLLWGRRLRPLLRAPALAAVPGGKPILCPRRTTAQLGPRRNPAWSLQAGRLFSTQTAEDKEEPLHSIISSTESVQGSTSKHEFQAETKKLLDIVARSLYSEKEVFIRELISNASDALEKLHHKLVSDGQALPEMEIHLQTNAEKGTITIQDTGIGMTQEELVSNLGTIARSGSKAFLDALQNQAEASSKIIGQFGVGFYSAFMVADRVEVYSRSAAPGSLGYQWLSDGSGVFEIAEASGVRTGTKIIIHLKSDCKEFSSEARVRDVVTKYSNFVSFPLYLNGRRMNTLQAIWMMDPKDVREWQHEEFYRYVAQAHDKPRYTLHYKTDAPLNIRSIFYVPDMKPSMFDVSRELGSSVALYSRKVLIQTKATDILPKWLRFIRGVVDSEDIPLNLSRELLQESALIRKLRDVLQQRLIKFFIDQSKKDAEKYAKFFEDYGLFMREGIVTATEQEVKEDIAKLLRYESSALPSGQLTSLSEYASRMRAGTRNIYYLCAPNRHLAEHSPYYEAMKKKDTEVLFCFEQFDELTLLHLREFDKKKLISVETDIVVDHYKEEKFEDRSPAAECLSEKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAKTQEERAQLLQPTLEINPRHALIKKLNQLRASEPGLAQLLVDQIYENAMIAAGLVDDPRAMVGRLNELLVKALERH

2cg9A

0.36

0.32

0.83

2.91

Download

-----------------------------------------------------------------------------------ASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSLSDPKQLPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSGTKAFMEALSAGAD--VSMIGQFGVGFYSLFLVADRVQVISKSNDDE--QYIWESNAGGSFTVTLDERIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEFVAYPIQLEIEELNKTKPLWTRNPSDITQEEYNAFYKSISNDWEDPLYVKHFSVEGQLEFRAILFIPKRAPF---------NNIKLYVRRVFITDEAEDLIPEWLSFVKGVVDSEDLPLNLSREMLQQNKIMKVIRKNIVKKLIEAFNEIAE-DSEQFEKFYSAFSKNIKLGVHEDT--QNRAALAKLLRYNSTKSV-DELTSLTDYVTRMPEHQKNIYYITGESLKAVEKSPFLDALKAKNFEVLFLTDPIDEYAFTQLKEFEGKTLVDITKD------FELEETDEEKAEREKEIKEYEPLTKALKEILGDQVEKVVVSYKLLDAPAAIRTGQFGWSANMERIMKAQ----------AKTFEISPKSPIIKELKKRVDEGGKTVKDLTKLLYETALLTSGFLDEPTSFASRINRLISLGLN--

2o1uA

0.33

0.29

0.79

3.03

Download

------------------------------------------------------------------------------------------------MMKLIINSLYKNKEIFLRELISNASDALDKIRLISLTDENALEELTVKIKCDKEKNLLHVTDTGVGMTREELVKNLGT----------------------------VGFYSAFLVADKVIVTSKHNNDT--QHIWESDSNEFSVIADPNTLGRGTTITLVLKEEASDYLELDTIKNLVKKYSQFINFPIYVDWELMNDIKPIWQRPSKEVEDDEYKAFYKSFSKESDDPMAYIHFTAEGEVTFKSILFVPTSAPR----------YIKLYVRRVFITDDFHDMMPKYLNFVKGVVDSDDLPLNVSRETLQQHKLLKVIRKKLVRKTLDMIKKIADE--KYNDTFWKEFGTNIKLGVIE--DHSNRTRLAKLLRFQSSHH-PSDITSLDQYVERMKEKQDKIYFMAGSSRKEAESSPFVERLLKKGYEVIYLTEPVDEYCIQALPEFDGKRFQNVAKEGVKFDES-----EKTKESREAIEKEFEPLLNWMKDALKDKIEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTGYYASQKKTFEINPRHPLIKDMLRRVKEDDKTVSDLAVVLFETATLRSGYLPDTKAYGDRIERMLRLSLNI-

2cg9A

0.36

0.32

0.83

7.64

Download

-----------------------------------------------------------------------------------ASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSLSDPKQLPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSGTKAFMEALSAGAD--VSMIGQFGVGFYSLFLVADRVQVISKSNDDE--QYIWESNAGGSFTVTLDERIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEFVAYPIQLVIEELNKTKPLWTRNPSDITQEEYNAFYKSISNDWEDPLYVKHFSVEGQLEFRAILFIPKRAPFN---------NIKLYVRRVFITDEAEDLIPEWLSFVKGVVDSEDLPLNLSREMLQQNKIMKVIRKNIVKKLIEAFNEIAE-DSEQFEKFYSAFSKNIKLGVHEDTQ--NRAALAKLLRYNSTKS-VDELTSLTDYVTRMPEHQKNIYYITGESLKAVEKSPFLDALKAKNFEVLFLTDPIDEYAFTQLKEFEGKTLVDITK------DFELEETDEEKAEREKEIKEYEPLTKALKEILGDQVEKVVVSYKLLDAPAAIRTGQFGWSANMERIMK----------AQAKTFEISPKSPIIKELKKRVDEGGKTVKDLTKLLYETALLTSGFLDEPTSFASRINRLISLGLN--

2cg9A

0.36

0.32

0.83

5.43

Download

-----------------------------------------------------------------------------------ASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSLSDPKQLPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSGTKAFMEALSAGA--DVSMIGQFGVGFYSLFLVADRVQVISKSNDDE--QYIWESNAGGSFTVTVNERIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEFVAYPIQLVVTKLNKTKPLWTRNPSDITQEEYNAFYKSISNDWEDPLYVKHFSVEGQLEFRAILFIPKRAP---------FNNIKLYVRRVFITDEAEDLIPEWLSFVKGVVDSEDLPLNLSREMLQQNKIMKVIRKNIVKKLIEAFNEIA-EDSEQFEKFYSAFSKNIKLGV--HEDTQNRAALAKLLRYNSTK-SVDELTSLTDYVTRMPEHQKNIYYITGESLKAVEKSPFLDALKAKNFEVLFLTDPIDEYAFTQLKEFEGKTLVDITKDFELEETDEEKAE------REKEIKEYEPLTKALKEILGDQVEKVVVSYKLLDAPAAIRTGQFGWSANMERIM----------KAQAKTFEISPKSPIIKELKKRVDAQDKTVKDLTKLLYETALLTSGFLDEPTSFASRINRLISLGLN--

2cg9A

0.36

0.32

0.83

4.55

Download

-----------------------------------------------------------------------------------ASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSLSDPKQLPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSGTKAFMEALSAGAD--VSMIGQFGVGFYSLFLVADRVQVISKSNDDE--QYIWESNAGGSFTVTLDEVIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEFVAYPIQLEIEELNKTKPLWTRNPSDITQEEYNAFYKSISNDWEDPLYVKHFSVEGQLEFRAILFIPKRAPF---------NNIKLYVRRVFITDEAEDLIPEWLSFVKGVVDSEDLPLNLSREMLQQNKIMKVIRKNIVKKLIEAFNEIAE-DSEQFEKFYSAFSKNIKLGVHEDT--QNRAALAKLLRYNSTKSV-DELTSLTDYVTRMPEHQKNIYYITGESLKAVEKSPFLDALKAKNFEVLFLTDPIDEYAFTQLKEFEGKTLVDITK------DFELEETDEEKAEREKEIKEYEPLTKALKEILGDQVEKVVVSYKLLDAPAAIRTGQFGWSANMERIMKAQ----------AKTFEISPKSPIIKELKKRVDEQDKTVKDLTKLLYETALLTSGFLDEPTSFASRINRLISLGLN--

2cg9A

0.36

0.32

0.83

2.70

Download

-------------------------------------------------------------------------------A----SETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSLSDPKQLEDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSGTKAFMEALSAGAD--VSMIGQFGVGFYSLFLVADRVQVISKSNDDEQ--YIWESNAGGSFTVTLNERIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEFVAYPIQLEIEELNKTKPLWTRNPSDITQEEYNAFYKSISNDWEDPLYVKHFSVEGQLEFRAILFIPKRAPF---------NNIKLYVRRVFITDEAEDLIPEWLSFVKGVVDSEDLPLNLSREMLQQNKIMKVIRKNIVKKLIEAFNEIAE-DSEQFEKFYSAFSKNIKLGV--HEDTQNRAALAKLLRYNSTK-SVDELTSLTDYVTRMPEHQKNIYYITGESLKAVEKSPFLDALKAKNFEVLFLTDPIDEYAFTQLKEFEGKTLVDITKDFELEETD------EEKAEREKEIKEYEPLTKALKEILGDQVEKVVVSYKLLDAPAAIRTGQFGWSANMERIMK-------------KTFEISPKSPIIKELKKRVDEQDKTVKDLTKLLYETALLTSGFLDEPTSFASRINRLISLGLN--

2o1uA

0.34

0.29

0.79

10.69

Download

------------------------------------------------------------------------------------------------MMKLIINSLYKNKEIFLRELISNASDALDKIRLISLTDENALAELTVKIKCDKEKNLLHVTDTGVGMTREELVKNLGT----------------------------VGFYSAFLVADKVIVTSKHN--NDTQHIWESDSNEFSVIADPRGLGRGTTITLVLKEEASDYLELDTIKNLVKKYSQFINFPIYVWWELMNDIKPIWQRPSKEVEDDEYKAFYKSFSKESDDPMAYIHFTAEGEVTFKSILFVPTSAPR----------YIKLYVRRVFITDDFHDMMPKYLNFVKGVVDSDDLPLNVSRETLQQHKLLKVIRKKLVRKTLDMIKKIA--DEKYNDTFWKEFGTNIKLGVIE--DHSNRTRLAKLLRFQSSHHPS-DITSLDQYVERMKEKQDKIYFMAGSSRKEAESSPFVERLLKKGYEVIYLTEPVDEYCIQALPEFDGKRFQNVAKEGV-----KFDESEKTKESREAIEKEFEPLLNWMKDALKDKIEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKYASQKKTFEINPRHPLIKDMLRRVKEDEKTVSDLAVVLFETATLRSGLLPDTKAYGDRIERMLRLSLNI-

2o1uA

0.33

0.29

0.79

2.76

Download

------------------------------------------------------------------------------------------------MMKLIINSLYKNKEIFLRELISNASDALDKIRLISLTDENALAELTVKIKCDKEKNLLHVTDTGVGMTREELVKNLGT----------------------------VGFYSAFLVADKVIVTSKHNNDTQ--HIWESDSNEFSVIADPNTLGRGTTITLVLKEEASDYLELDTIKNLVKKYSQFINFPIYVDWELMNDIKPIWQRPSKEVEDDEYKAFYKSFSKESDDPMAYIHFTAEGEVTFKSILFVPTSAPR----------YIKLYVRRVFITDDFHDMMPKYLNFVKGVVDSDDLPLNVSRETLQQHKLLKVIRKKLVRKTLDMIKKIADEKY--NDTFWKEFGTNIKLGV--IEDHSNRTRLAKLLRFQSSH-HPSDITSLDQYVERMKEKQDKIYFMAGSSRKEAESSPFVERLLKKGYEVIYLTEPVDEYCIQALPEFDGKRFQNVAKEGVKF-----DESEKTKESREAIEKEFEPLLNWMKDALKDKIEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTNYYASQKKTFEINPRHPLIKDMLRREDEDDKTVSDLAVVLFETATLRSGYLPDTKAYGDRIERMLRLSL---

2cg9A

0.36

0.32

0.83

7.11

Download

-----------------------------------------------------------------------------------ASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSLSDPKQLPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSGTKAFMEALSAGA--DVSMIGQFGVGFYSLFLVADRVQVISKSNDD--EQYIWESNAGGSFTVTLDERIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEFVAYPIQLVIEELNKTKPLWTRNPSDITQEEYNAFYKSISNDWEDPLYVKHFSVEGQLEFRAILFIPKRAPFN---------NIKLYVRRVFITDEAEDLIPEWLSFVKGVVDSEDLPLNLSREMLQQNKIMKVIRKNIVKKLIEAFNEIAE-DSEQFEKFYSAFSKNIKLGVHEDT--QNRAALAKLLRYNSTKS-VDELTSLTDYVTRMPEHQKNIYYITGESLKAVEKSPFLDALKAKNFEVLFLTDPIDEYAFTQLKEFEGKTLVDITKD------FELEETDEEKAEREKEIKEYEPLTKALKEILGDQVEKVVVSYKLLDAPAAIRTGQFGWSANMERIMK----------AQAKTFEISPKSPIIKELKKRVDEGGATVKDLTKLLYETALLTSGFLDEPTSFASRINRLISLGLN--

2o1uA

0.33

0.29

0.79

2.75

Download

------------------------------------------------------------------------------------------------MMKLIINSLYKNKEIFLRELISNASDALDKIRLISLTDENALAELTVKIKCDKEKNLLHVTDTGVGMTREELVKNLGTV----------------------------GFYSAFLVADKVIVTSKHNNDT--QHIWESDSNEFSVIARGNTLGRGTTITLVLKEEASDYLELDTIKNLVKKYSQFINFPIYVWWELMNDIKPIWQRPSKEVEDDEYKAFYKSFSKESDDPMAYIHFTAEGEVTFKSILFVPTSAP----------RYIKLYVRRVFITDDFHDMMPKYLNFVKGVVDSDDLPLNVSRETLQQHKLLKVIRKKLVRKTLDMIKKIA--DEKYNDTFWKEFGTNIKLGVIED--HSNRTRLAKLLRFQSSHH-PSDITSLDQYVERMKEKQDKIYFMAGSSRKEAESSPFVERLLKKGYEVIYLTEPVDEYCIQALPEFDGKRFQNVAKEGVKF-----DESEKTKESREAIEKEFEPLLNWMKDKLKDKIEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTNYYASQKKTFEINPRHPLIKDMLRRVKEDEKTVSDLAVVLFETATLRSGYLPDTKAYGDRIERMLRLSLNI-

(a)	All the residues are colored in black; however, those residues in template which are identical to the residue in the query sequence are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade. (more about the colors used)
(b)	Rank of templates represents the top ten threading templates used by I-TASSER.
(c)	Ident1 is the percentage sequence identity of the templates in the threading aligned region with the query sequence.
(d)	Ident2 is the percentage sequence identity of the whole template chains with query sequence.
(e)	Cov. represents the coverage of the threading alignment and is equal to the number of aligned residues divided by the length of query protein.
(f)	Norm. Z-score is the normalized Z-score of the threading alignments. Alignment with a Normalized Z-score >1 mean a good alignment and vice versa.
(g)	Download Align. provides the 3D structure of the aligned regions of the threading templates.
(h)	The top 10 alignments reported above (in order of their ranking) are from the following threading programs:
	1: MUSTER 2: HHSEARCH 3: SP3 4: PROSPECT2 5: PPA-I 6: HHSEARCH I 7: FUGUE 8: HHSEARCH II 9: SPARKS 10: MUSTER

10 proteins in PDB which are structurally closest to the first I-TASSER model (identified by TM-align)

Rank	TM-score	RMSD^a	IDEN^a	Cov.	PDB Hit
1	0.8266	0.77	0.36	0.83	2cg9A Model1

2	0.5713	4.13	0.20	0.65	2o1uA Model1

3	0.3928	3.85	0.12	0.45	1ei1A Model1

4	0.3881	3.87	0.12	0.45	1s16A Model1

5	0.3840	4.38	0.21	0.44	1y4sA Model1

6	0.3754	4.57	0.13	0.45	1kijA Model1

7	0.3719	4.91	0.09	0.45	2q2eB Model1

8	0.3663	4.54	0.13	0.44	1mu5A Model1

9	0.3583	4.27	0.12	0.42	1pvgA Model1

10	0.3567	4.34	0.14	0.42	1b63A Model1

Structural alignment using TM-align
-----------------------------------------------------------------------------------ASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSLSDPKQLETEPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSGTKAFMEALSAGA--DVSMIGQFGVGFYSLFLVADRVQVISKSNDDE--QYIWESNAGGSFTVTLDEVNERIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEFVAYPIQLVVTKEVEKEEEVQEIEELNKTKPLWTRNPSDITQEEYNAFYKSISNDWEDPLYVKHFSVEGQLEFRAILFIPKRAPF---------NNIKLYVRRVFITDEAEDLIPEWLSFVKGVVDSEDLPLNLSREMLQQNKIMKVIRKNIVKKLIEAFNEIAE-DSEQFEKFYSAFSKNIKLGVHED-T-QNRAALAKLLRYNSTKSV-DELTSLTDYVTRMPEHQKNIYYITGESLKAVEKSPFLDALKAKNFEVLFLTDPIDEYAFTQLKEFEGKTLVDITKDFELEE------TDEEKAEREKEIKEYEPLTKALKEILGDQVEKVVVSYKLLDAPAAIRTGQFGWSANMERIMKAQA----------KTFEISPKSPIIKELKKRVDEGGAQDKTVKDLTKLLYETALLTSGFSLDEPTSFASRINRLISLGLN-- MARELRALLLWGRRLRPLLRAPALAAVPGGKPILCPRRTTAQLGPRRNPAWSLQAGRLFSTQTAEDKEEPLHSIISSTESVQGSTSKHEFQAETKKLLDIVARSLYSEKEVFIRELISNASDALEKLHHKLVSDGQAL---PEMEIHLQTNAEKGTITIQDTGIGMTQEELVSNLGTIARSGSKAFLDALQNQAEASSKIIGQFGVGFYSAFMVADRVEVYSRSAAPGSLGYQWLSDGSGVFEIAEA--S-GVRTGTKIIIHLKSDCKEFSSEARVRDVVTKYSNFVSFPLYLN-------------GRRMNTLQAIWMMDPKDVREWQHEEFYRYVAQAHDKPRYTLHYKTDAPLNIRSIFYVPDMKPSMFDVSRELGSSVALYSRKVLIQTKATDILPKWLRFIRGVVDSEDIPLNLSRELLQESALIRKLRDVLQQRLIKFFIDQSKKDAEKYAKFFEDYGLFMREGIVTATEQEVKEDIAKLLRYESSALPSGQLTSLSEYASRMRAGTRNIYYLCAPNRHLAEHSPYYEAMKKKDTEVLFCFEQFDELTLLHLREFDKKKLISVETDIVVDHYKEEKFEDRSPAAECLSEKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAKTQEERAQLLQPTLEINPRHALIKKLNQLRASE---PGLAQLLVDQIYENAMIAAGLV-DDPRAMVGRLNELLVKALERH

MMKLIINSLYKNKEIFLRELISNASD-----------------------------------------------------------------------------------------------------------ALDKIRLISLTDENALAG-NEEL----------------TVKIKCDKEKNLL----HVTDTGVGMTREELVKNLGTVGFYSAFLVADKVIVTS------------------------------------------------KHNNDTQHIWESDSNEFSVIADPRG-------------------------------------NTLGRG------TTITLVLKEEASDYLELDTIKNLVKK-------YSQFINFPIYVWSSKVWDWELMNDIKPIWQRPSKEVEDDEYKAFYKSFSKESDDPMAYIHFTAEGEVTFKSILFVPTSAP-R---------YIKLYVRRVFITDDFHDMMPKYLNFVKGVVDSDDLPLNVSRETLQQHKLLKVIRKKLVRKTLDMIKKI--ADEKYNDTFWKEFGTNIKLGVIED-H-SNRTRLAKLLRFQSS-HHPSDITSLDQYVERMKEKQDKIYFMAGSSRKEAESSPFVERLLKKGYEVIYLTEPVDEYCIQALPEFDGKRFQNVAKEGVK---FDESEKTKESREAIEKEFEPLLNWMKD--KALKDKIEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIKDMLRRVKEDEDDKTVSDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNI- --------------------------MARELRALLLWGRRLRPLLRAPALAAVPGGKPILCPRRTTAQLGPRRNPAWSLQAGRLFSTQTAEDKEEPLHSIISSTESVQGSTSKHEFQAETKKLLDIVARSLYSEKEVFIRELI----SNA-SDALEKLHHKLVSDGQALPEMEIHLQTN------AEKGTITIQD-------------------------------TGIGMTQEELVSNLGTIARSGSKAFLDALQNQAEASSKIIGQFGVGFYSAFMVADR-----------------VEVYSRSAAPGSLGYQWLSDGSGVFEIAEASGVRTGTKIIIHLKSDCKEFSSEA---------------------RVRDVVTKYSNFVSFPLYLN-----GRRMNTLQAIWMMDPKDVREWQHEEFYRYVAQAHDKPRYTLHYKTDAPLNIRSIFYVPDMKPSMFDVSRELGSSVALYSRKVLIQTKATDILPKWLRFIRGVVDSEDIPLNLSRELLQESALIRKLRDVLQQRLIKFFIDQSKKDAEKYAKFFEDYGLFMREGIVTATEQEVKEDIAKLLRYESSALPSGQLTSLSEYASRMRAGTRNIYYLCAPNRHLAEHSPYYEAMKKKDTEVLFCFEQFDELTLLHLREFDKKKLIS-VETDIVVDHYKEEKFEDRSPAAECLSEKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAK---T--Q-EERAQLLQPTLEINPRHALIKKLNQLR-AS-EPGLAQLLVDQIYENAMIAAGLVDD-PRAMVGRLNELLVKALERH

SNSSDSSSIK------------------------------------------------------------------------------------------VLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGH-----------CKEIIVTIHA-DNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGGKF-DDNS------Y-KVSGGLHGVGVSVVNALSQKLELVIQREG--KIHRQIYEHGV-PQAPLAVTGETEKTGTMVRFWPSLETFTNVTEF-EYEILAKRLRELSFLDSGVSIRLRDKRDGKEDHFHYEGGI------------K-AFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWN-D-G--F------QENIYCFTNNIPQRDGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSSQTKDKLVSSEVKSAVEQQMNELLAEYLLEN--PT--DAKIVVGKIIDA-AR------------------------------------------------------------------------------AREAA-R-----------------------------------------------------------------------------------------R--AREMT-------------------------------------------------------------------------- ----------MARELRALLLWGRRLRPLLRAPALAAVPGGKPILCPRRTTAQLGPRRNPAWSLQAGRLFSTQTAEDKEEPLHSIISSTESVQGSTSKHEFQAETKKLLDIVARS-LYS----EK-EVFIRELISNASDALEKLHHKLVSDGQALPEMEIHLQTNAEKGTITIQDTGIG-MTQ-------EELVSNLGTIARSGSKAFLDALQNQAEASSKIIGQFGVGFYSAFMVADRVEVYSRSAAPGSLGYQWLSDGSGVFEIAEASGVR--TGTKIIIHLKS----DCKEFSSEARVRDVVTKYSNFV-SFPLYLN------GRRMNTLQAIWMMDPKDVREWQHEEFYRYVAQAHDKPR-YTLHYKTDAP-LNIRSIFYVPDMKPSMFDVSRELGSSVALYSRKVLIQTKA--TD-IL-------------------------PKWLRF-IRGVVDSED-IPLNL-S-RELLQESALIRKLRDVLQQRLIKFFIDQSKKDAEKYAKFFEDYGLFMREGIVTATEQEVKEDIAKLLRYESSALPSGQLTSLSEYASRMRAGTRNIYYLCAPNRHLAEHSPYYEAMKKKDTEVLFCFEQFD-ELTLLHLREFDKKKLISVETDIVVDHYKEEKFEDRSPAAECLSEKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAKT-QEERAQLLQPTLEINPRHALIKKLNQLRASEPGLAQLLVDQIYENAMIAAGLVDDPRAMVGRLNELLVKALERH

TYNADAIE------------------------------------------------------------------------------------------VLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGH-----------AKRVDVILHA-DQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKF-SNKN------Y-QFSGGLHGVGISVVNALSKRVEVNVRRDG--QVYNIAFENGE-KVQDLQVVGTCGKRNTGTSVHFWPDETFFDSPRF-SVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDGL------------N-DYLAE-AVNGLPTLPEKPFIGNFAGDT-EAVDWALLW-LPEGG------ELLT-ESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFILWLNQNV--Q--AA--EL-LAEMAISSA-------------------------------------------------------------------------------QRRM------------------------------------------------------------------------------------------R--AA--------------------------------------------------------------------------- --------MARELRALLLWGRRLRPLLRAPALAAVPGGKPILCPRRTTAQLGPRRNPAWSLQAGRLFSTQTAEDKEEPLHSIISSTESVQGSTSKHEFQAETKKLLDIVARS-LYSEK-EVFIRELISNASDALEKLHHKLVSDGQALPEMEIHLQTNAEKGTITIQDTGIG-MTQ-------EELVSNLGTIARSGSKAFLDALQNQAEASSKIIGQFGVGFYSAFMVADRVEVYSRSAAPGSLGYQWLSDGSGVFEIAEASGVR----TGTKIIIHLKS--D-CKEFSSEARVRDVVTKYSNFVS-FPLYLN------GRRMNTLQAIWMMDPKDVREWQHEEFYRYVAQAHD-K-PR-YTLHYKTDAPLNIRSIFYVPDMKPSMFDVSRELGSSVALYSRKVLIQTKA--TDI-L------------------------PKWLR-FIRGVVDSED-IPLNL-S-RELLQESALIRKLRDVLQQRLIKFFIDQSKKDAEKYAKFFEDYGLFMREGIVTATEQEVKEDIAKLLRYESSALPSGQLTSLSEYASRMRAGTRNIYYLCAPNRHLAEHSPYYEAMKKKDTEVLFCFEQFDELTLLHLREFDKKKLISVETDIVVDHYKEEKFEDRSPAAECLSEKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAKTQEERAQLLQPTLEINPRHALIKKLNQLRASEPGLAQLLVDQIYENAMIAAGLVDDPRAMVGRLNELLVKALERH

MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD-----------------------------------------------------------------------------------------------------------KLRFRALSNPDLYEGDG-----------------ELRVRVSFDKDKRTLTIS---------DNGVGMTRDEVIDHLGTIAKSGTKSFLESSQLIGQFGVGFYSAFIVADKVTVRTRA------------------------------------------------------------------------------------------------AGEKPENGVFWESAGEGEYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSD------HIALPVEIEKREEKDGETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTSLLYIPSQAPWDMW-NRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDS-SDLPLNVSREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAED-F-ANQEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELLRKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSV---------------------------------------------------------------------------------------------------------------------------------------------------------- --------------------------------------------MARELRALLLWGRRLRPLLRAPALAAVPGGKPILCPRRTTAQLGPRRNPAWSLQAGRLFSTQTAEDKEEPLHSIISSTESVQGSTSKHEFQAETKKLLDIVARSLYSEKEVFIRELISNASDALEKLHHKLVSDGQALPEMEIHLQ-------------TNAEKGTITIQD-----------------------------------------------------TGIGMTQEELVSNLGTIARSGSKAFLDALQNQAEASSKIIGQFGVGFYSAFMVADRVEVYSRSAAPGSLGYQWLSDGSGVFEIAEASGVRTGTKIIIHLKSDCKE--------------FSSEARVRD---------------------------VVTKYSNFVSFPLYL----------N-GRRMNTLQAIWMMDPKDVREWQHEEFYRYVAQAHDKPRYTLHYKTDAPLNIRSIFYVPDMKPSMFDVSRELGSSVALYSRKVLIQTKATDILPKWLRFIRGVVDSEDIPLNLSRELLQ-ESALIRKLRDVLQQRLIKFFIDQSKKDAEKYAKFFEDYGLFMREGIVTATEQEVKEDIAKLLRYESSALPS-GQLTSLSEYASRMRAGTRNIYYLCAPNRHLAEHSPYYEAMKKKDTEVLFCFEQFDELTLLHLREFDKKKLISVETDIVVDHYKEEKFEDRSPAAECLSEKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAKTQEERAQLLQPTLEINPRHALIKKLNQLRASEPGLAQLLVDQIYENAMIAAGLVDDPRAMVGRLNELLVKALERH

AIR------------------------------------------------------------------------------------------VLKGLEGVRHRPAMYIGGTGVEGYHHLFKEILDNAVDEALAGY-----------ATEILVRLNE-DGSLTVEDNGRGIPVDLMPEEGKPAVEVIYNTLHSG-GKFEQ-G--------AYKVSGGLHGVGASVVNALSEWTVVEVFREG--KHHRIAFSRG--EVTEPLRVVGEAPRGKTGTRVTFKPDPEIFGNLR-F-DPSKIRARLREVAYLVAGLKLVFQDRQHGKEEVFL-DKGGV-----------A-SFAKALAEGEDLLYEKPFLIRGTHGEVEVEVGFLHTQGY---N------AEILTYAN--MIPTRDGGTHLTAFKSAYSRALNQYAKKAGLNKEKGPQPTGDDLLEGLYAVVSVKLPNPQFEGQTKGKLLNPEAGTAVGQVVYERLLEILEENPR-IAKAVYE-KALRA-A------------------------------------------------------------------------------QAREAAR-----------------------------------------------------------------------------------------KARELV--------------------------------------------------------------------------- ---MARELRALLLWGRRLRPLLRAPALAAVPGGKPILCPRRTTAQLGPRRNPAWSLQAGRLFSTQTAEDKEEPLHSIISSTESVQGSTSKHEFQAETKKLLDIVARS-LYS---EK-EVFIRELISNASDALEKLHHKLVSDGQALPEMEIHLQTNAEKGTITIQDTGIG-MT-------QEELVSNLGTIARSGSKAFLDALQNQAEASSKIIG-Q-FGVGFYSAFMVADRVEVYSRSAAPGSLGYQWLSDGSGVFE-IAEASGVR----TGTKIIIHL-K---SDCKEFSSEARVRDVVTKYSNFV-SFPLYLN------GRRMNTLQAIWMMDPKDVREWQHEEFYRYVAQAHDKPR--YTLHYKTDAPLNIRSIFYVPDMKPSMFDVSRELGSSVALYSRKVLIQTKAT--DI--L-------------------------PKWLRF-IRGVVDSEDI-PLNLSRELLQESALIRKLRDVLQQRLIKFFIDQSKKDAEKYAKFFEDYGLFMREGIVTATEQEVKEDIAKLLRYESSALPSGQLTSLSEYASRMRAGTRNIYYLCAPNRHLAEHSPYYEAMKKKDTEVLFCFEQFDELTLLHLREFDKKKLISVETDIVVDHYKEEKFEDRSPAAECLSEKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAKTQEERAQLLQPTLEINPRHALIKKLNQLRASEPGLAQLLVDQIYENAMIAAGLVDDPRAMVGRLNELLVKALERH

KK---------------------------------------------------------------------------------------QKSISVAEFFEKNRQILGFDSAPRSLITTVKEAVDNALDACEEAGI----------LPDILVQVERTGPDYVTVIIEDNGPGIVREQIPKVFAKLLYG--F--HA------L--K-SRGQQGIGISAAVLYAQMTAGRHTKILSKTSPTAPAHYYELMINTSTNEPDILVDEVRDWFRPHGTQIELEMR-AAYVKGRR-QSIYEYLKATAIVNPHARITLIDPDGNEEVFERATDKMPEPAEEILPHPEGIELGTLMKMLHYTEPFLRYSFCKLLTAEEICKAAGLDPEIDPHALGRHEARKLIEAFEKVKIMAPPTDCLSPIGED-LI---YR-GLEKET-TV--DF--IATSTRKPAVYSGNPFVVEVGMAYGGNLPKEE-----KISI-MRFANRVPLLYQQGGCVTTHAVEDIKWKQYGLNQPGGGIPVGPVILLIHVASINVPFTSESKDAIADIPVIKEEIDLAIKEVARKLKHYL--S-KQSNLKKRREKEIIITKVLPKLAAKVAHVLEKDVPDINPVV--------------------------------AKIMGNLLVHRVIKNNGDGTVDVAIKVK--------NAYSFRVHEMLPCKVKPEPKVVTMGNDYDYVWDISASA---------------------------------GSSKVLSYKIESASEEELQKLPQLIVTGAK------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- M-ARELRALLLWGRRLRPLLRAPALAAVPGGKPILCPRRTTAQLGPRRNPAWSLQAGRLFSTQTAEDKEEPLHSIISSTESVQGSTSKHEFQAETKKLLDIVARS--LYS--EK-EVFIRELISNASDALEKLHHKLVSDGQALPEMEIHLQTNAEK---GTITIQDTGIGMTQEELVSNLGTIARSGSKAFLDALQNQAEASSKIIGQFGVGFYSA-FMV--ADR-VEVYSRSAA--PGSLGYQWLSDGSGV-FEI-AEASG--V-RTGTKIIIHLKSDCKEFSS-EARVRDVVTKYSNFV-SFPLYLN----GRRMNTLQA-I-------------------------------------------------------------------------------------WMMDPKDVREWQHEEFYRYVAQAHDKPRYTLHYKTDAP------LNIRSIFYVPDMKPSMFDVSRELGSSVALYSRKVLIQ--T----KATDIL-PKWL-----------RF--IRGVVDSEDI-PLN--LSRELLQESALIRKLRDVLQQRLIKFFIDQSKKDAEKYAKFFE---------------------------------DYGLFMREGIVTATEQEVKEDIAKLLRYESSALPSGQ-----------------------LTSLSEYASR------------------------------------MRAGTRNIYYLCAPNRHLAEHSPYYEAMKKKDTE-----------------------------VLFCFEQFDELTLLHLREFDKKKLISVETDIVVDHYKEEKFEDRSPAAECLSEKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAKTQEERAQLLQPTLEINPRHALIKKLNQLRASEPGLAQLLVDQIYENAMIAAGLVDDPRAMVGRLNELLVKALERH

-------------------------------------------------------------------------------------------LSPAEFFKRNPELAGFPNPARALYQTVRELIENSLDATDV-HG-I--------LPNIKITIDLIDDARQIYKVNVVDNGIGIPPQEVPNAFGRVLYSK-YV--N-------R--Q-TRGMYGLGVKAAVLYSQMHQDKPIEIETSPVNSKRIYTFKLKIDINKNEPIIVERGSVENTRGFHGTSVAISIP-GDWPKAK-SRIYEYIKRTYIITPYAEFIFKDPEGNVTYYPRLTNKIPKPPQEVKPHPYGVDREEIKILINNLKRDYTIKEFLVNEFQSIGDTTADKILELAGLKPNKKVKNLTEEEITRLVETFKKYEDFRSPSADSLSVIGED-LI---EL-GLKKIF--N--PD-FAASITRKPKAYQGHPFIVEAGVAFGGSIPVGE------EPIVLRYANKIPLIYDEKSDVIWKVVEELDWKRYGIESDQYQMVVMVHLCSTKIPYKSAGKESIAEVEDIEKEIKNALMEVARKLKQYLSEKRKEQEAKK--------------------------------------KLLA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- MARELRALLLWGRRLRPLLRAPALAAVPGGKPILCPRRTTAQLGPRRNPAWSLQAGRLFSTQTAEDKEEPLHSIISSTESVQGSTSKHEFQAETKKLLDIVARS--LYS--EK-EVFIRELISNASDALEKLHHKLVSDGQALPEMEIHLQTNAEK-----GTITIQDTGIGMTQEELVSN-LGTIARSGSKAFLDALQNQAEASSKIIGQFGVGFYSA-FMV--AD-RVEVYSRSA--APGSLGYQWLSDGSG-VFEI-AEASGVR----TGTKIIIHLKSDCKEFSSEARVRDVVTKYSNFV-SFPLYLN-----GRRMNTLQ-AI---------------------------------------------------------------------------------------------WMMDPKDVREWQHEEFYRYVAQAHDKPRYTLHYKTDAP------LNIRSIFYVPDMKPSMFDVSRELGSSVALYSRKVLIQ--T----KATDILPKWL----R----F-IRGVVDSEDIPLNL--S-RELLQESA-LIRKLRDVLQQRLIKFFIDQSKKDAEKYAK-FFEDYGLFMREGIVTATEQEVKEDIAKLLRYESSALPSGQ--LTSLSEYASRMRAGTRNIYYLCAPNRHLAEHSPYYEAMKKKDTEVLFCFEQFDELTLLHLREFDKKKLISVETDIVVDHYKEEKFEDRSPAAECLSEKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAKTQEERAQLLQPTLEINPRHALIKKLNQLRASEPGLAQLLVDQIYENAMIAAGLVDDPRAMVGRLNELLVKALERH

SASDKYQ------------------------------------------------------------------------------------------KISQLEHILKRPDTYIGSVETQEQLQWIYDEETDCIEKNVTIVPGLFKIFDEILVNAADNKVRDP--S---------KRIDVNIHAEEHTIEVKNDGKGIPIEIHNKENIYIPEIFGHLL-TSSNY-DDD-E----K--KVTGGRNGYGAKLCNIFSTEFILETADLNVGQKYVQKWENNS-ICHPPKITSYKKGPSYTKVTFKPDLTRFGKELDNDI-LGVRRRVYDINGSVRDINVYLNGKSLKIRNFK--------------NYVELYLKSLIPTILYERIN-----NRWEVAFAV--S--DI---S--FQQ-ISFVNSIATTGGTHVNYITDQIVKKISEILKKVKSFQIKNNFIFINCLIENPAFTSQTKEQLTT-RVK-DFGS--RC-EIP-LEYINKI-----K--TDLATRFEI-A-------------------------------------------------------------------------------D-A----------------------------------------------------------------------------------------------------------------------------------------------------------------------------- -------MARELRALLLWGRRLRPLLRAPALAAVPGGKPILCPRRTTAQLGPRRNPAWSLQAGRLFSTQTAEDKEEPLHSIISSTESVQGSTSKHEFQAETKKLLDIVARS-LYS-------------------------EK-EVFIRELISNASDALEKLHHKLVSDGQALPEMEIHLQTNAEKGTITIQDTGIGM-TQ-------EELVSNLGTIARSGSKAFLDALQNQAEASSKIIGQFGVGFYSAFMVADRVEVYSRSAAPGSLGYQWLSDGSGVFEIAEAS-G--VRTGTKIIIHLKS----DCKEFSSEARVRDVVTKYSNFVS-FPLYLNGRRMNTLQAIWMMDPKDVREWQHEEFYRYVAQAHDKPRYTLHYKTDAPLNIRSIFYVPDMKPSMFDVSRELGSSVALYSRKVLIQTKA-TD--IL--------------PKWL-RFIRGVVDSEDIPLN-LS-RELLQESALIRKL--RDVLQQRLIKFFIDQSKKDAEKYAKFFEDYGLFMREGIVTATEQEVKEDIAKLLRYESSALPSGQLTSLSEYASRMRAGTRNIYYLCAPNRHLAEHSPYYEAMKKKDTEVLFCFEQFDELTLLHLREFDKKKLISVETDIVVDHYKEEKFEDRSPAAECLSEKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAKTQEERAQLLQPTLEINPRHALIKKLNQLRASEPGLAQLLVDQIYENAMIAAGLVDDPRAMVGRLNELLVKALERH

SHMPIQ------------------------------------------------------------------------------------------VLPPQLANQIAAGEVVERPASVVKELVENSLDAGA---------------TRIDIDIERGGAKLIRIRDNGCGIKKDELALALARH-ATSKIASLDDLE-----AI--I-SLGFRGEALASISS-VSRLTLTSRTAEQQEA-WQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKFLRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYR-AVP--EG---GQ---KERRLGAICGTAFLEQALAIEWQHGD--LTLRGWVADPNHTTPA-----LAEIQYCYVNGRMMRDRLINHAIRQACEDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRF-HQSRLVHDFIYQGVLSVL--Q----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- ------MARELRALLLWGRRLRPLLRAPALAAVPGGKPILCPRRTTAQLGPRRNPAWSLQAGRLFSTQTAEDKEEPLHSIISSTESVQGSTSKHEFQAETKKLLDIVARSLYSEKEVFIRELISNASDALEKLHHKLVSDGQALPEMEIHLQTNA-EKGTITIQDTGIGMTQEELVSNLGTIARSGSKAFLDA--LQNQAE-ASSKIIGQFGVGFYSAFMVADRVEVYSRSAAPGSLGYQWLSDGSGVFEIAEASGVRTGTKIIIHLK-S-----D--C-KEFSSEARVRDVVTKYSNFV-SFPLYLN----G-RRMNTLQAIWMMDPKDVREWQHEEFYRYVAQAHDKPRYTLHYKTDAPLNIRSIFYVPDMKPSMFDVSRELGSSVALYSRKVLIQTK--ATDILP-KWL-----R-FIRGVVDSEDI--P-L-NLS-RELLQESALIRKLRDVLQQRLIKFFIDQSKKDAEKYAKFFEDYGLFMREGIVTATEQEVKEDIAKLLRYESSALPSGQLTSLSEYASRMRAGTRNIYYLCAPNRHLAEHSPYYEAMKKKDTEVLFCFEQFDELTLLHLREFDKKKLISVETDIVVDHYKEEKFEDRSPAAECLSEKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAKTQEERAQLLQPTLEINPRHALIKKLNQLRASEPGLAQLLVDQIYENAMIAAGLVDDPRAMVGRLNELLVKALERH

(a)	Residue pairs that are structurally aligned by TM-align program are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade.(more about the colors used)
(b)	Ranking of proteins is based on TM-score of the structural alignment between Model1 and the PDB structures in our template library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.

Function Prediction

Predicted EC Numbers

Rank	TM-score	RMSD^a	IDEN^a	Cov.	EC-Score	PDB Hit	EC No.
1	0.3928	3.85	0.12	0.45	0.4110	1ei1A	5.99.1.3

2	0.3671	4.55	0.13	0.44	0.4003	2hkjA	5.99.1.3

3	0.3653	4.21	0.11	0.43	0.3648	1q1dB	5.99.1.3

4	0.3583	4.49	0.11	0.43	0.3573	1zxnD	5.99.1.3

5	0.3719	4.91	0.09	0.45	0.3398	2q2eB	5.99.1.3

(a)	Ranking is based on EC-score, which is a confidence score for the Enzyme Classification (EC) Number prediction.
(b)	RMSD^a is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)	IDEN^a is percentage sequence identity in the structurally aligned region.
(d)	Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)	EC-Score is defined based on the C-score of the structure prediction and similarity of the model with known enzyme structures, as identified using both global and local structural alignment programs. The global similarity score uses TM-score, IDEN^a,RMSD^a and Cov. of the structural alignment by TM-align, while the local match compares the structural and chemical similarity of local spatial motifs in the model with known catalytic site of enzymes. A prediction with a EC-score >1.1 signifies a prediction with high confidence (upto 3 digit numbers of EC) and vice versa (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).

Predicted GO terms

Rank	TMscore	RMSD^a	IDEN^a	Cov.	PDB Hit	Fh-Score	Associated GO Terms
1	0.3320	1.67	0.28	0.34	1y6zB	0.56	GO:0005524 GO:0030554 GO:0051082 GO:0006457 GO:0006950 GO:0009987 GO:0034960 GO:0043283 GO:0044237
2	0.3851	4.19	0.19	0.43	2o1wB	0.52	GO:0005509 GO:0005524 GO:0030554 GO:0043169 GO:0051082 GO:0006457 GO:0009987 GO:0034960 GO:0043283 GO:0044237 GO:0005622 GO:0005623 GO:0005788 GO:0016528 GO:0016529 GO:0031410 GO:0031974 GO:0042470 GO:0043229 GO:0044444 GO:0044446
3	0.2469	1.91	0.38	0.26	2o1wB	0.49	GO:0005509 GO:0005524 GO:0030554 GO:0043169 GO:0051082 GO:0006457 GO:0009987 GO:0034960 GO:0043283 GO:0044237 GO:0005622 GO:0005623 GO:0005788 GO:0016528 GO:0016529 GO:0031410 GO:0031974 GO:0042470 GO:0043229 GO:0044444 GO:0044446
4	0.2421	2.03	0.37	0.25	2vwcA	0.47	GO:0005524 GO:0030554 GO:0042623 GO:0051082 GO:0009987 GO:0010926 GO:0044237 GO:0050789 GO:0050896 GO:0065007 GO:0005622 GO:0005623 GO:0005737
5	0.2419	2.03	0.36	0.25	1uymA	0.46	GO:0005524 GO:0030235 GO:0030554 GO:0030911 GO:0051082 GO:0001890 GO:0006457 GO:0006809 GO:0006986 GO:0009058 GO:0009607 GO:0009653 GO:0009887 GO:0009891 GO:0009893 GO:0009987 GO:0031325 GO:0031328 GO:0034960 GO:0043283 GO:0044249 GO:0044271 GO:0045429 GO:0048518 GO:0048522 GO:0048856 GO:0051173 GO:0051789 GO:0065007 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0031410 GO:0042470 GO:0043229 GO:0044444
6	0.2424	1.98	0.36	0.25	3d0bA	0.46	GO:0005524 GO:0030235 GO:0030554 GO:0030911 GO:0042802 GO:0042803 GO:0051082 GO:0006809 GO:0006839 GO:0006986 GO:0007154 GO:0007165 GO:0009058 GO:0009607 GO:0009891 GO:0009893 GO:0010926 GO:0016044 GO:0022607 GO:0031325 GO:0031328 GO:0034619 GO:0034621 GO:0034622 GO:0034960 GO:0042026 GO:0043283 GO:0043623 GO:0044249 GO:0044271 GO:0045429 GO:0048518 GO:0048522 GO:0051173 GO:0051641 GO:0051649 GO:0051789 GO:0065007 GO:0070096 GO:0070271 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0031410 GO:0042470 GO:0043229 GO:0043231
7	0.2386	2.29	0.36	0.25	2iorA	0.45	GO:0005524 GO:0030554 GO:0051082 GO:0006457 GO:0006950 GO:0009987 GO:0034960 GO:0043283 GO:0044237 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886
8	0.3870	3.84	0.12	0.45	1s16B	0.44	GO:0003677 GO:0003918 GO:0005524 GO:0030554 GO:0006265 GO:0009987 GO:0034960 GO:0043283 GO:0044237 GO:0046677 GO:0005622 GO:0005623 GO:0005694 GO:0043229 GO:0044424
9	0.3779	4.32	0.12	0.45	1kijB	0.43	GO:0003677 GO:0003918 GO:0005524 GO:0030554 GO:0006265 GO:0009987 GO:0034960 GO:0043283 GO:0044237 GO:0005622 GO:0005623 GO:0005694 GO:0043229 GO:0044424
10	0.3569	4.29	0.14	0.42	1nhjA	0.43	GO:0005524 GO:0030554 GO:0030983 GO:0006261 GO:0006298 GO:0009058 GO:0009987 GO:0033554 GO:0034960 GO:0034961 GO:0043283 GO:0043284 GO:0044249 GO:0045005 GO:0051716

Consensus Prediction of Gene Ontology terms

Molecular Function    Biological Process Cellular Location

GO term GO-Score    GO term GO-Score GO term GO-Score

GO:0032553 0.472    GO:0044237 0.519    GO:0044424 0.453

GO:0005488 0.472    GO:0009987 0.472    GO:0005623 0.453

GO:0032559 0.472    GO:0043170 0.425    GO:0044464 0.453

GO:0030554 0.472    GO:0034960 0.425    GO:0005622 0.453

GO:0017076 0.472    GO:0044260 0.425    GO:0043229 0.361

GO:0005524 0.472    GO:0043283 0.425    GO:0043226 0.361

GO:0000166 0.472    GO:0044238 0.425    GO:0005737 0.286

GO:0032555 0.472    GO:0050896 0.328    GO:0044444 0.238

GO:0005515 0.341    GO:0006457 0.294    GO:0031410 0.193

GO:0051082 0.341    GO:0019538 0.294    GO:0031988 0.193

(a) Ranking in the first table is based on a function prediction score (Fh-score), which is calculated based on the C-score of the structure prediction and the TM-score, IDEN^a, RMSD^a and Cov. of the structural alignment by TM-align between the predicted model and the PDB structures (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).

(b) RMSD^a is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.

(c) IDEN^a is the percentage sequence identity in the structurally aligned region.

(d) Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.

(e) A consensus prediction of GO terms is derived from the structural analogs that have an Fh-score of >=1.0. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on the Fh-score of the template from which the GO term is derived. A prediction with a GO-score >0.5 signifies a prediction with high confidence and vice versa.

Predicted Binding Site

Binding site residues in the model:

MET:	448	ARG:	449	ILE:	452	VAL:	453	THR:	454	THR:	456	GLU:	457
GLN:	458	GLU:	459	LYS:	461	GLU:	462	ILE:	464	ALA:	465	LYS:	466
LEU:	467	LEU:	468	ARG:	469	SER:	482	LEU:	483	LEU:	534	THR:	535
LEU:	536	HIS:	538	LEU:	539	ARG:	540	GLU:	541	PHE:	542

Identified analogs with similar binding site:

Rank PDB
Hit TM-score RMSD^a IDEN^a Cov. BS-score Download
Complex Binding site residues on the predicted model

1 1jzqA 0.2663 8.88 0.04 0.45 0.91 Download 448,449,452,453,454,456,457,458,459,461,462,464,465,466,467,468,469,482,483,534,535,536,538,539,540,541,542

2 2bf4A 0.2733 7.59 0.06 0.41 0.79 Download 329,331,340,341,342,343,344,363,380,383,384,389,390,391,415,416,417,418,419,420,421,422,423,424,425,448,451,457,460,464

3 2bf4A 0.2733 7.59 0.06 0.41 0.70 Download 114,277,280,297,298,299,311,312,315,316,324,325,326,327,328,329,330,331,339,340,341,342,343,344,345,363,364,365,366,367,369,370,371,372,373,374,388,389,390,391,392,393,402,425

4 1j1wA 0.2929 8.03 0.04 0.46 0.67 Download 114,198,199,332,333,334,335,336,337,338,339,340,365,366,367,368,369,370,371,372,379,391,392,393,394,395,396,397,402,414

5 1osfA 0.2417 2.08 0.36 0.25 0.42 Download 119,120,122,123,126,161,163,167,168,171,172,177,178,179,180,181,197,198,199,200,202,203,204,205,206,217,251

6 2gqpA 0.2434 1.80 0.39 0.25 0.35 Download 119,120,123,161,162,163,167,168,169,171,172,173,174,175,202,203,205,206,219,231,251

7 2p1pB 0.2602 7.76 0.05 0.40 0.32 Download 384,423,426,441,444,445,447,448,449,452,464,467

8 2p1pB 0.2602 7.76 0.05 0.40 0.31 Download 348,349,350,351,352,353,354,355,356,357,359,386,442,443,444,446,447,529

9 2zbkB 0.3633 4.67 0.10 0.43 0.28 Download 116,119,120,122,123,126,158,161,162,163,167,179,205,219,251,253

10 2bf4A 0.2733 7.59 0.06 0.41 0.27 Download 12,108,272,275,276,277,315,317,318,319,361,362,363,364,373,374,388,390,403

(a) Ranking of the analogs in the table is based on their BS-score. BS-score is calculated by first finding the structural analogs of the predicted model based on their TM-score, IDEN^a and Cov. of the structural alignment. The ligand(s) in the analog structure are then transferred onto the model and the fitness of the ligand-model complex (BS-score) is calculated by comparing the local structure and sequence similarity in the binding site region. (For detail, see Kucukural, Szilagyi, Ambrish, and Zhang, Template based ligand binding site prediction on modeled protein structure, 2009, in preparation).

(b) A BS-score of >0.5 signifies a binding site prediction with high confidence and vice-versa.
(c) RMSD^a the RMSD between residues that are structurally aligned by TM-align.

(d) IDEN^a is the percentage sequence identity in the structurally aligned region.

(e) Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.

(f) The image shows the ligand-protein complex with the best BS-score. The ligand is depicted in magenta colored ball & stick, the predicted binding site residues interacting with the ligand are shown as transparent green spheres, while the N & C terminus in the model are marked by blue and red spheres respectively.

	Please cite following articles when you use the I-TASSER server:
1.	Yang Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9:40 (2008).
2.	Yang Zhang. Template-based modeling and free modeling by I-TASSER in CASP7. Proteins, 8: 108-117 (2007).
3.	Sitao Wu, Jeffrey Skolnick, Yang Zhang. Ab initio modeling of small proteins by iterative TASSER simulations. BMC Biology, 5:17 (2007).