I-TASSER results for job id S34343

Submitted Sequence

>Gag MGVRNSVLSGKKADELEKIRLRPNGKKKYMLKHVVWAANELDRFGLAESLLENKEGCQKI LSVLAPLVPTGSENLKSLYNTVCVIWCIHAEEKVKHTEEAKQIVQRHLVVETGTTETMPK TSRPTAPSSGRGGNYPVQQIGGNYVHLPLSPRTLNAWVKLIEEKKFGAEVVPGFQALSEG CTPYDINQMLNCVGDHQAAMQIIRDIINEEAADWDLQHPQPAPQQGQLREPSGSDIAGTT SSVDEQIQWMYRQQNPIPVGNIYRRWIQLGLQKCVRMYNPTNILDVKQGPKEPFQSYVDR FYKSLRAEQTDAAVKNWMTQTLLIQNANPDCKLVLKGLGVNPTLEEMLTACQGVGGPGQK ARLMAEALKEALAPVPIPFAAAQQRGPRKPIKCWNCGKEGHSARQCRAPRRQGCWKCGKM DHVMAKCPDRQAGFLGLGPWGKKPRNFPMAQVHQGLMPTAPPEDPAVDLLKNYMQLGKQQ REKQRESREKPYKEVTEDLLHLNSLFGGDQ

Predicted Secondary Structure

Sequence	20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                    |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |            MGVRNSVLSGKKADELEKIRLRPNGKKKYMLKHVVWAANELDRFGLAESLLENKEGCQKILSVLAPLVPTGSENLKSLYNTVCVIWCIHAEEKVKHTEEAKQIVQRHLVVETGTTETMPKTSRPTAPSSGRGGNYPVQQIGGNYVHLPLSPRTLNAWVKLIEEKKFGAEVVPGFQALSEGCTPYDINQMLNCVGDHQAAMQIIRDIINEEAADWDLQHPQPAPQQGQLREPSGSDIAGTTSSVDEQIQWMYRQQNPIPVGNIYRRWIQLGLQKCVRMYNPTNILDVKQGPKEPFQSYVDRFYKSLRAEQTDAAVKNWMTQTLLIQNANPDCKLVLKGLGVNPTLEEMLTACQGVGGPGQKARLMAEALKEALAPVPIPFAAAQQRGPRKPIKCWNCGKEGHSARQCRAPRRQGCWKCGKMDHVMAKCPDRQAGFLGLGPWGKKPRNFPMAQVHQGLMPTAPPEDPAVDLLKNYMQLGKQQREKQRESREKPYKEVTEDLLHLNSLFGGDQ
Prediction	CCCCCCCCCCCCCCHHHHHCCCCCCCCCCSCHHHCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCHCCCCCCHHHHHHHHHHHCSSSSSSCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCSSCCCCCCCCCCCSSSSSSCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHSSSSCCCCCSSSCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCSSCCCCCCCCCCCSSSSCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCC
Conf.Score	984210135666202455214788763021033401355667660584100033879999997501012373999988753140444244321352177887787775310013788999887414677655777753356644304678513030235654047796100114542389974788888753121899999999776077765540488779998666888887656776688999777632899975006668887422011256307741244266889713778888888877767568776543432311147875889998618887799999871568885188999999997631534011002566788862564058860043430578656666335866463355676445601005788999987667888889999998434454433121357778899963112565566786156686517899

Top 5 Models predicted by I-TASSER

Top 10 templates used by I-TASSER

Rank	PDB Hit	Iden1	Iden2	Cov.	Norm. Z-score	Download Align.		20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                    |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |
							Sec.Str Seq	CCCCCCCCCCCCCCHHHHHCCCCCCCCCCSCHHHCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCHCCCCCCHHHHHHHHHHHCSSSSSSCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCSSCCCCCCCCCCCSSSSSSCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHSSSSCCCCCSSSCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCSSCCCCCCCCCCCSSSSCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCC MGVRNSVLSGKKADELEKIRLRPNGKKKYMLKHVVWAANELDRFGLAESLLENKEGCQKILSVLAPLVPTGSENLKSLYNTVCVIWCIHAEEKVKHTEEAKQIVQRHLVVETGTTETMPKTSRPTAPSSGRGGNYPVQQIGGNYVHLPLSPRTLNAWVKLIEEKKFGAEVVPGFQALSEGCTPYDINQMLNCVGDHQAAMQIIRDIINEEAADWDLQHPQPAPQQGQLREPSGSDIAGTTSSVDEQIQWMYRQQNPIPVGNIYRRWIQLGLQKCVRMYNPTNILDVKQGPKEPFQSYVDRFYKSLRAEQTDAAVKNWMTQTLLIQNANPDCKLVLKGLGVNPTLEEMLTACQGVGGPGQKARLMAEALKEALAPVPIPFAAAQQRGPRKPIKCWNCGKEGHSARQCRAPRRQGCWKCGKMDHVMAKCPDRQAGFLGLGPWGKKPRNFPMAQVHQGLMPTAPPEDPAVDLLKNYMQLGKQQREKQRESREKPYKEVTEDLLHLNSLFGGDQ
1	1l6nA	0.55	0.31	0.56	2.19	Download		-GARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFAVNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKIEEEQNK--SKKKAQQAAADT-GNNSQVSQNYPVQNLQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGPPGQMREPRGSDIAGTTSTLQEQIGWMT-HNPPIPVGEIYKRWIILGLNKIVRMYSPTSIL--HHHHHH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
2	1l6nA	0.55	0.31	0.56	0.53	Download		-GARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFAVNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKIEEE---QNKSKKKAQQAAADTGNNSQVSQNYPVQNLQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGAPGQMREPRGSDIAGTTSTLQEQIGWM-THNPPIPVGEIYKRWIILGLNKIVRMYSPTSILHHHHHH----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
3	2eiaA	0.29	0.12	0.39	4.97	Download		------------------------------------------------------------------------------------------------------------------------------------------------------PRGYTTWVNTIQTNGLLNEASNLFGILSVDCTSEEMNAFLDVVPGQAGQKQILLDAIDKIADDWDNRHPLPNAPPQGPIPMTARFIRGLGVPRERQMEPAFD-----QFRQTYRQWIIEAMSEGIKMIGKPKAQNIRQGAKEPYPEFVDRLLSQIKSEGHPQEISKFLTDTLTIQNANEECRNAMRHLRPEDTLEEKMYACRDIG-----------------------------------------------------------------------------------------------------------------------------------------------------------
4	1l6nA	0.55	0.31	0.56	3.83	Download		-GARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFAVNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKIEEE---QNKSKKKAQQAAADTGNNSQVSQNYPVQNLQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGPPGQMREPRGSDIAGTTSTLQEQIGWM-THNPPIPVGEIYKRWIILGLNKIVRMYSPTSILH----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HHHHH
5	1l6nA	0.55	0.31	0.56	7.18	Download		-GARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFAVNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKIEEEQNKSKKKAQQAAADT---GNNSQVSQNYPVQNLQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGPPGQMREPRGSDIAGTTSTLQEQIGWMT-HNPPIPVGEIYKRWIILGLNKIVRMYSPTSILHHHHHH----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
6	1l6nA	0.55	0.31	0.56	1.27	Download		-GARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFAVNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKIEEE---QNKSKKKAQQAAADTGNNSQVSQNYPVQNLQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGPPGQMREPRGSDIAGTTSTLQEQIGWM-THNPPIPVGEIYKRWIILGLNKIVRMYSPTSILHHHHHH----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
7	1ed1A	0.95	0.22	0.22	5.71	Download		-----SVLSGKKADELEKIRLRPGGKKKYMLKHVVWAANELDRFGLAESLLENKEGCQKILSVLAPLVPTGSENLKSLYNTVCVIWCIHAEEKVKHTEEAKQIVQRHLVVET-----------GTAETMP--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
8	1l6nA	0.56	0.31	0.55	1.39	Download		-GARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFAVNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKIEEE---QNKSKKKAQQAAADTGNNSQVSQNYPVQNLQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGPPGQMREPRGSDIAGTTSTLQEQIGWM-THNPPIPVGEIYKRWIILGLNKIVRMYSPTSIL----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
9	2eiaA	0.29	0.12	0.39	4.39	Download		------------------------------------------------------------------------------------------------------------------------------------------------------PRGYTTWVNTIQTNGLLNEASNLFGILSVDCTSEEMNAFLDVVPGQAGQKQILLDAIDKIADDWDNRHPLPNAPPQGPIPMTARFIRGLGVPRERQMEPAFD-----QFRQTYRQWIIEAMSEGIKMIGKPKAQNIRQGAKEPYPEFVDRLLSQIKSEGHPQEISKFLTDTLTIQNANEECRNAMRHLRPEDTLEEKMYACRDIG-----------------------------------------------------------------------------------------------------------------------------------------------------------
10	1e6jP	0.69	0.28	0.41	1.45	Download		------------------------------------------------------------------------------------------------------------------------------------------------VHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGPPGQMREPRGSDIAGTTSTLQEQIGWMT-NNPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQG-------------------------------------------------------------------------------------------------------------------------------------------------------------
(a) All the residues are colored in black; however, those residues in template which are identical to the residue in the query sequence are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade. (more about the colors used) (b) Rank of templates represents the top ten threading templates used by I-TASSER. (c) Ident1 is the percentage sequence identity of the templates in the threading aligned region with the query sequence. (d) Ident2 is the percentage sequence identity of the whole template chains with query sequence. (e) Cov. represents the coverage of the threading alignment and is equal to the number of aligned residues divided by the length of query protein. (f) Norm. Z-score is the normalized Z-score of the threading alignments. Alignment with a Normalized Z-score >1 mean a good alignment and vice versa. (g) Download Align. provides the 3D structure of the aligned regions of the threading templates. (h) The top 10 alignments reported above (in order of their ranking) are from the following threading programs:         1: MUSTER   2: HHSEARCH   3: SP3   4: PROSPECT2   5: PPA-I   6: HHSEARCH I   7: FUGUE   8: HHSEARCH II   9: SPARKS   10: MUSTER

10 proteins in PDB which are structurally closest to the first I-TASSER model (identified by TM-align)

Rank TM-score RMSDa IDENa Cov. PDB Hit 1 0.5482 1.28 0.54 0.56 1l6nA Model1   2 0.3645 7.87 0.04 0.63 1llwA Model1   3 0.3417 7.83 0.04 0.58 1kcwA Model1   4 0.3390 7.11 0.04 0.54 1ffyA Model1   5 0.3260 7.43 0.04 0.53 1rlrA Model1   6 0.3252 7.70 0.06 0.55 1mjgM Model1   7 0.3234 7.12 0.04 0.51 1z1wA Model1   8 0.3212 7.42 0.04 0.52 3ecqA Model1   9 0.3204 3.68 0.54 0.37 1e6jP Model1   10 0.3185 7.18 0.06 0.51 2iw3A Model1

Structural alignment using TM-align   -GARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFAVNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKIEEE---QNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWM-THNPPIPVGEIYKRWIILGLNKIVRMYSPTSILH---------------------------------------------------------------------------------------------------------------------H----------------HHHH----------------------------------------------------------------------------------------  MGVRNSVLSGKKADELEKIRLRPNGKKKYMLKHVVWAANELDRFGLAESLLENKEGCQKILSVLAPLVPTGSENLKSLYNTVCVIWCIHAEEKVKHTEEAKQIVQRHLVVETGTTETMPKTSRPTAPSSGRGGNYPVQ-QIGGNYVHLPLSPRTLNAWVKLIEEKKFGAEVVPGFQALSEGCTPYDINQMLNCVGDHQAAMQIIRDIINEEAADWDLQHPQPAPQ-QG-QLREPSGSDIAGTTSSVDEQIQWMYRQQNPIPVGNIYRRWIQLGLQKCVRMYNPTNILDVKQGPKEPFQSYVDRFYKSLRAEQTDAAVKNWMTQTLLIQNANPDCKLVLKGLGVNPTLEEMLTACQGVGGPGQKARLMAEALKEALAPVPIPFAAAQQRGPRKPIKCWNCGKEGHSARQCRAPRRQGCWKCGKMDH-VMAKCPDRQAGFLGLGPWGKKPRNFPMAQVHQGLMPTAPPEDPAVDLLKNYMQLGKQQREKQRESREKPYKEVTEDLLHLNSLFGGDQ    CGVGFIANLRGKP--DHTLVEQALKALGCMEHRGGCSADNDSGDGAGVMTAIPRELLAQWFNTRNLPMPDGDRLGVGMVFLPQEPSAREVARAYVEEVVRLEKLTVLGWREVPVNSDVLGIQAKNNQPHIEQILVTCPEGCAGDELDRR-------------LYIARSIIGKKLAEDFYVCSFSCRTIVYKGMVRSIILGEFYLDLKNPGYTSNFAVYHRRFSTNTMPKWPLAQPMRLLGHNGEINTLLGNINWMAAREKELEVSGWTKAELEALTPIVNQANSDSYNLDSALELLVRTGRSPLEAAMILVPEAYKNQPALKDYPEISDFHDYYSGLQEPWDGPALLVFSDGKIVGAGLDRNGLRPARYCITKDDYIVLGSEAGVVDLPEVDIVEKGRLAPGQMIAVDLAEQKILKNYQIKQQAAQKYPYGEWIKIQDAQ--------------TVLQQQAAFGYTAEDVEM--VVVPMASQGKEPTFCMGDDTPLAVLSHK------PRLLYDYFKQRFAQVTNPPI-------------DPLRENLVMS-LAMFLGKRGNLLEPKAEKLRS---PLVNEVELQAIKTGQLQVAEVSTLYDLDGVNSL---------EDALTNLVKTAIATVQAGAE------ILVLTDRPNGAILTENQSFIPPLLAVGAVHHHLIRAGLRLKASLIVDTAQCWSTHHFACLVGYGASAICPYLALESVRQWWLDEKTQKLMENGRLDRIDLPTALKNYRQSVEAGLF-KILS--KMGISLLASYHGAQIFEAIGLGAELVEYAFAGTTSRVGGLTIADVAGEVMVFHGMAFPEMAKKLENFGF-VNYRPGGEYHM---NSPEMSKSLHKAVAAYYDHYELYRQYLKDRPVTALRDLLDFNADQPAISLEEVESV----ESIVKR-FCT--G--GM----SLGALSREAHETLAIAMNRLGAKSNSGEGGEDVVRYLTLDDVDSEGNSPTLPHLHGLQNGDTANSAIKQIASGRFGVTPEYLMSGKQLEIKMAQGAKPGEGGQLPGKKVSEYIAMLRRSKPGVTLISPPPH---------------------------------HDIYSIED--LAQLIYDLHQINPEAQVS-VKLVA--EI-GIGTIAAGVAKANADIIQISGHDGGTGASPLSSIKHAGSPWELGVTEVHRVLMENQLRDRVLLRADGGLKTGWDVVMAALMGAEEYGFGSIAMIAEGCIMARVCHTNNCPVGVATQQERLRQRFKGVPGQVVNFFYFIAEEVRSLLAHLGYRSLDDIIGRTDLLKVRSDVQLSKTQNLTLDCLLNLPDTKQNRQWLNHEPV--HSNGPVLDDDILADPDIQEAINHQTTATKTYRLVNTDRTVGTRLSGAIAKKYGNNGFEGNITLNFQGAAGQSFGAFNLDGMTLHLQGEANDYVGKGMNGGEIVIVPHPQASFAPEDNVI-IGNTCLYGATGGNLYANGRAGERFAVRNSVGKAVIEGAGDHCCEYMTGGVIVVLGPVGRNVGAGMTGGLAYFLDEVGDLPEKINPEIITLQRITASKGEEQLKSLITAHVEHTGSPKGKAILANWSDYLGKFWQAVPPSEKDSPEANN--------------------------------------------------------  -------------MGVRN-SV-LSGKKADELEKIRLRPNGKKK-YMLKHV--V-----------------------WAA--------------------------------N-----------------ELDRFGL-------------AESLLENKEGCQKILSVLAPLVPTGSENLKSLY-------NTVCV-I--W----------------CIHAEEKVK-H-TE--EAK-----QIVQRH---------------------------------------LVVETG-TT-------------ETMP-K------------------T--SRPT--------------------------------------------------------------------------------------A-PSSG-----------RGGNYPVQQIGGNYVHL-PL---------S--PRTLNAWVKLIEEKKFGAEVVP--------GFQALSEGC-----------------TPYDINQMLNCVGDHQAAM-QI-IR--------------------DIIN--------------------------------EEAADWDLQHP-QPAPQQGQLREP-----SGSDIAGTTSSVDEQIQWMYRQQNPIPV-------------------------GNIYRRWIQLG-------------------------------------------------------L--QKC--VRM--------------------------------------------------------------------YN----------PTNI--------------------------------L----------------------DVKQGPKEPFQSYVDRFYKSLRAEQTDAAVKNWMTQTLLIQNANPDCKL-------------VL--K---GL------GVNPT-LEEMLTAC---------------QGVGG--------------------------------------------PGQKARLMAEALKEALAPVPIPFAAAQQRGPRKPIKCWN--CGK-----------------EGHSARQCRAPRRQGCWKC-G---------------------------------KMDHVM--------AKCPDRQ-----------------------------------------------------------------------------------------------------------------------------------------AGFLGL-G-------------------------------PW-------------------------------GK------------------KPR--------------------------NFPM----------------AQV--------------HQ--------------------------------------------------------------------------------------------------------------GLMPTAPPEDPAVDLLKNYMQLGKQQREKQRESREKPYKEVTEDLLHLNSLFGGDQ    KEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPDRIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHSHGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDG----NCVTRIYHSHIDAPKDIASGLIGPLIICKKDSLDKEKEKH-IDRE-F-VVMFSVVDENFSWYLEDNIKTYCSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPG-LSM-CAEDR-V--KW-YL--FGMGNEVDVHAAFFHGQAL-TNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFFQVQECIRGKHVRHYYIAAEEII---------------------------------WN-YAPSGIDIFTKENLTAPGSDSAVFFE--------------------QGTTRIGG---SY-KKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAYPLSIEPIGVRFNKNNEGTYYSPVPPSASHV------APTETFTYEWTVPKEVGPTNADPVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENESLLLEDNI--RMFTTAPDQVDKEDEDFQES-------------------NK-MHSMNGFMYGNQPGLTMCKGDSVVWYLFSAGNEADVHGIYFS---GNTYLWRGERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDHYTGG--MK-QK--YTV-N-QCRRQ-SEDSTFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQNVSNAFLDKGEFYIGSKYKKVVYRQYTDSTFRVPVERKAEEEHLGILGPQLHADVGDKVKIIFKNMATRPYSIHAHGVQ------TESSTVTPTLPGETLT-YVWKIPERSGAGTEDSACIPWAYYSTVDQVKDLYSGLIGPL--IVCRRP-----RRKLEFALLFLVFDENESWYLDDNIKTYSDHPEKVNKDDEEFIESNKMHAINGRMFGNLQGLTMHVGDEVNWYLMGMGNEIDLHTVHFHGHSFQYKHRGVYSSDVFDI--------------------------------------FPG-TYQTLEMFPRTPGIWL-LHCHVTDHIHAGMETTYTVLQ-N---------------------------------------------  MG---------------------------------------------------------------------------------VRNSV-------------LSGKK-----------------------------------A--DELEKIRLRPNGKKKY-----------------------MLK-HVVWAANEL-DRFGLAESLLENK-E------------------------------------GCQKI---LSVLAPLVPTGSENLKSLYNTVCVIWCIH---AEEK--------VKHTEEAKQIVQRH--L-V-------------VETGTTETMPKTSR-------------------------PTAPSSGRGGNYPVQQIGGNYVHLPLSPRTLNAWVKLIEEKKFG------------------AEVVPGFQALSEGCTPYDINQMLNCVGDHQAAMQIIRDI----------------------INE----------------E-AADWDLQH-----------P-----QPA-----PQQGQLREPS-----------------------------G---SD--I-------------------------------------AGTTSSV--DEQI------------------QWMYRQQNPIPVGNIYRRWI-QLGLQKCVRMYNPTNILDVKQGP--------------------KEPFQS-----------------------YVDRFYKSLRAEQ----TD--AAVKNWMTQTLLIQNANPDCKLVL--------------------------------------------------------------------------------------------------------------------KGLGVNPTLEE-----------MLTACQGVGGPG---QKARLM---------------------AEA-----LKEALAPVPIPFAAAQQR-----------------------------------GP---------------------------RKPIKC-------------------------WNCGKEGHSARQCRAPRRQGCWKCGKMDHVMAKCPDRQAG-FLGLGPWG---------KKPRNFPMAQVHQGLMPTAPPEDPAVDLLKNYMQLGKQQREKQRESREKPYKEVTEDLLHLNSLFGGDQ    MDYEKTLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKNKGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAP-------YVPGW--DTHGL--------------------PIEQALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDP-----YITLKPEYEAAQIRIFGEMADKGLIYKGKKPVYWSPSSESS-----------LAEAEI--EY--HDK-------RSASIY------------VAFNVKDDKGVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALDWDKASIKLEKEYTGKELE---W--VV--AQHPFLDR-ES--LV--INGDHVTTDAGTGCVHTAPGHGEDDYIVGQQYELPVISPIDDKGVFTEEG----GQFEGMFYDKANKAVTDLLTEKGALLKLDFITHSYPHDWRTKKPVIFR---------------------ATPQWFAS------------ISKVRQ-------DI-LDAIE---------------------------------------------------N--TNF--K---------------VNWGK-TRIYN------------MVRDRGE-WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGFTHPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNS-----SITTSVATRGV-SPYKFLLSHG-FVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSSTDYLADVRISDEILKQTSDDYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLLNRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRSMQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKWRTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVSQVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVKSLV-------------------  ------------------------MGVRN-------------------------------SVLSGKKADELE-K---------------IRLRPNGKKKYMLKHV---VWAANELDRFGLAESLLENKEGCQKILSVLAPL--V-PTGSENLKSLYNTVCVIWCI-H-------------AEEKVKHTEE------------------------------------AKQIVQRHLVVETGTTETMPKTSRPTAPSSGRGG-----NYPVQQIGGNYVHLPLS---------PRTL--------------------------N-----AWV------------------------KLIEEKKFGAEVVPGFQALSEGCTP-YDINQMLNCVGDHQAAM--Q---IIRDIINE---E---------AADWDLQHPQPA---PQQGQLREPSGSDIAGTTSSVDEQIQ--------------------------WMYRQQNPIPVGNIYRRWIQLG-------LQKCVRMYNPTNILDVKQGPKEPFQSYVDRFYKSLRAEQTDAAVKNWMTQTLLIQNANPDCKLVLKGLGVNPTLEEMLTACQGVGGPGQKARLMAEALKEALAPVPIPFAAA-QQ----RGPRKPIKCWNCGKEGHSARQCRAPR------------------------------------------------------------------RQGC-WKC-GK--------------------------------MDHVMAKCPDRQA---GFLGLGPWGKKPRNFP-MAQVHQ----GLM-P--T--------APP------------------------ED-PAVDLLKN-YM---------------------------------------------------------------Q-LGK-QQR-------------------------------------------------------------------------------------------------------------------------------------------------------------EK-Q--------------RESREKP-----YKEVTEDLLHLNSLFGGDQ    RDGSTERINLDKIHRVLDWAAEGLHNVSISQVELRSHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLA----------------------ARLAIFHLRKKAYGQFE-----PPALYDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEG-----KYLVQNRVTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSIN-ATS-SAIVKYVSQRAGIGINAGRIRAL----------------GSPIRGGEAFHTGCIPFYKHFQTAVKSCSQGGVRGGAATLFYPM----WHLEVESLLVLKNNRGVEGNRVRHMDYGVQ---------INKLMYTRLLKGEDITL-FSPSDVPGLYDAFFADQEE--FERLYTKYEKDDSI-------------------------------------------RK--QR---VKAVE------LFS--LMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLNDVNDENGEIALCTLSAFNLGAINNLDELDELAILAVRALDALLDYQDYPIPAAKRGAMGRRTLGIGVIN----FAYYLAKHGKRYSD-GSANNLTHKTFEAIQYYLLKASNELAKEQGACPWFNETTYAKGILPIDTYKK--DLDTIANE-------------------PL--HYDW-------------------------------EALRESIKTHGLRNSTLS--A-------LMPS--ETSSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLVGIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDDIDDLSNFQL------------------------------------------------------------------------------------  ------------------------------------------------------------------------MGVRNSVLSGKKADELEKIRLRPN--G------------KKKYMLKHVVWAANELDRFGLAESLLENKEG-----------------------------CQKILSVLAPLVPTGSENLKS-LYNTVCVIWCIHA--------EE--KV-KHT-E-EAKQIVQR-HLV---VETGTTET-----------MPK-TSRP--------------------TAPSSGRGGNYPVQQIGG-----NYVHLP-------------------------------LSPRTL----------------------------NAWVKLIEEKKFGAEVVPG-FQALSEGCTPYDINQMLNCVGDHQ--AAM-------------QIIRDIINEEAADWDLQHPQPAPQQGQLREPSGSDIAGTTSSVD-EQI-QWMY----RQQNPIP--VGNIYRRW----IQLGLQKCVRMYN------------------------------------------------------------------------------------------------PTNILD-VKQGPKE----PFQSYVDRFYKSLR-A------------------------------EQTDAAVKNWMTQTLLIQNANPDCKLVLKGLGVNPTLEEML---TACQGVGGPGQKARLMAEALKEALAPVPIPFAAA--QQ-RGPRKPIKCWNCGKEGHSARQCRAPRRQGCWK--CGK--MDHV-------------------------MAK-------------------------------------------------------------------------------CPDRQAGFLGLGPWGKKPRNFPMAQVHQGLMPTAPPEDPAVDLLKNYMQLGKQQREKQRESREKPYKEVTEDLLHLNSLFGGDQ    TDFDKIFEGAIPEGKEPVALFREVYHGAITATSYAEILLNQAIRTY----------------------------------------------------------G-PDHPVGYPDT-----------------------------AYYLPVIRCFS----------GEEVKKLGDLPPILNRKRAQVSPVLNFENARLAGEATWYAAE--IIEALRYLKYKPDEPLLPPPWTGFIGDPVVRRFGIKMVDWTIPGEAIILG--------------------RAKDSK-ALAKIVKELM-GMGF---M--LF------ICDEAVEQLLEENVKLGIDYIAYPLGNFTQIVHAANYALRAGMMFGGVTPGAREEQ--RDYQRRRIRAFVLYLGEH----------DMVKTAAAFGAIFTGFPV-------ITD-QPLPEDKQIPDWFFSVEDYDKIVQIAMETRGIKLTKIKLDLPINFGPAF-EGESIRKGDMYVEMGGNRTPAFELVRTVSESEITDGKIEVIGPDIDQIPEGSKLPLGILVDIYGRKMQADFEGVLERRIHDFINYGEGLWHTGQRNINWLRVSKDAVAKGFRFKNYGEILVAKMKEEFPAIVDRVQVTIFTDEAKVKEYMEVAREKYKERDDRMRGLTDETV----------DTFYSCVLCQSFAPNHVCIVTPERVGLCGA-V-SWLDAKASYEINHAGP--NQ--------------PI-PKEGEIDPIKGIWKSVNDYLYTASNRNLEQVCLYTLMENPMTSCGCFEAIMAILPECN-----G-IMITTR-DHAGMTPSGMTFSTLAG-----------------M------------IGGGTQTPGFMGIGRTYIVSKKFISADGGIARIVWMPKSLKDFLHDEFVRSSVEEGLGEDFIDKI-ADETIGTTVDEILPYLEEKGHPALTMDPIM---------------------------------------------------  ----------------------------------------------MGVRNSVLSGKKADELEKIRLRPNGKKKYMLKHVVWAANELDRFGLAESLLENKEGCQKILS--------VLAPLVPTGSENLKSLYNTVCVIWCIHAEE----------KVKHTEEAKQIVQRHLVVET--------------------------------GTTETMPKTSRPT---------------------------------------APSSGRGGNYPVQQIGGNYVHLPLSPRTLNAWVKLIEEKKFGAEVVPGF-QALSEGCTPYDINQML-NCVGDHQA-AMQII-----RDIINEEAA-------------D---WDLQ----------------HPQPAPQQGQLREPSGSDIAG-------TTSSVDEQIQWMYRQQNPI----PVGNI----YR--RWIQLGLQK-----------CVR-MY-------------------------------------------------------------------------------N--------------------------------------------PTN--------------------------------------------ILDVKQGPKEPFQSYV-----------DRFYK-SLRAEQTDAAVKNWMTQTLLIQNAN-PDCKLVLKGLGVNPTLEEMLTACQGVGGPGQK----------------------ARLMA-------------EALKEALAPVPIPFAAAQQ-RGPRKP-----IKCWNCGKEGHSARQCRAPRRQGCWKCGKMDHVMAKCPDRQAGFLGL-------------G--P----WGKKPR----------------------------NFPM-AQVHQGLMPT----------------APPEDPAVDLLKNYMQLGKQQREKQRESREKPYKEVTEDLLHLNSLFGGDQ    MEVEKYDLTLDFDIQKRTFNGTETITADAGDIVLDAVGLQINWMKVNGRDTAFTYDGQTVRAPGDSQPQKIEISFAGKVSDSLSGIYYAGRENGMITTHFEATDARRMFPCVDHPAYKAVFAITVVIDKDYDAISNMPPKRIEVSERKVVEFQDTPRMSTYLLYVGIGKFRYEYEKYRDIDLILASLKDIRSKYPLDMARKSVEFYENYFGIPYALPKMHLISVPEF--GAGAMENWGAITFREIYMDIAENSAVTVKRNSANVIAHEIAHQWFGDLVTM-------KWWNDLWLNESFATFMSYKTMDTLFPEWSFWGDFFVSRTSGALRSDSLKNTHPIEVDVRDPDEISQIFDEISYGKGASILRMIEDYAGYEEFRKGISKYLNDHKFGNA-----EGSDLWTAIEDVSGKPVKRVMEY---------------------WIKNPGYPVIKLKRNGRKITMYQTRFLLNGEEEGRWPVPVNIKKKDGVERILL-EDEASIEADGLIKINADSAGFYRVL----------------------------------------------------------------------YDDATFSDVMGHYRDLSPLDRIGLVDDLFAFLLSGHIDPE--------------------------------------------TYR--QRIRNFFDDEDHNVITAIVGQMEYLRMLTHAFDDDARAFCRSRMQFLTGKQDENL-------KIA--LGRVSRLYVMVDESYAEEMSKLFKDFDSAEPEMRSSIATAYAL------------VTGDLKGLLEKFRSVDRDEDRVRIISAFGKLKSNTDLSTVYGMVEKTE---------------IKKQDMISFFSSALETLPGREFIFANLDRIIRLVIRYFTGN------------------------------------------------------RTASRTVEMMIPVIGLDHPDAEDIVRNIGSKNISMGLAKGIEMLAVNRKLVERIRQTAVK-------------------------------------------------------  --------------------------------------------------------------------------------------------------------M-----GVRNSV-------------------------------------------LSG-----------------------------------------------------------------KKADELE-------K-----------------IRLRPNGKKKYMLKHVVWAANELDRFGLAESLLEN----------------------------------------------KEG---------------C---QK-IL-SVLA-PLVPTGSENLKSLY--------NTVCVIW---------------------CIHAEEKVKHTEEAKQIVQRHLVVETGTTET-----------MPKTS----RPTAPS-----------------SGRGG---------------------NYPVQQIGGNYVHLPLSPRTLNAWVKLIEEKKFGAEVVPGFQALSEGCTPYDINQMLNCVGDHQAAMQIIRDIINEEAADWDLQH-------------------------PQPAPQQGQLREPSGSDIAGTTSSVDEQIQWMYRQQNPIPVGNIY--RRWIQLGLQK-----------------------C-VRMYNPTN--IL-DVKQG----PKEPFQSY--VDRFYKSLRAEQTDAAVKNWMTQTLLIQNANP-------------DCKLVLKGLGVNPTLE-EML-TACQGVGGPGQK---------------------------ARLMAEALKEALAPVPIPFAAAQQR-------------------------------GPRKPIKCWNCGKEGHSARQCRAPRRQGCWKCGKMDHVMAKCPDRQAGFLGLGPWGKKPRNFP-M-------------------AQVHQG------------------------LMPTAPPEDPAVDLLKNYMQLGKQQREKQRESREKPYKEVTEDLLHLNSLFGGDQ    KTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAGKPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTKNNVFVGYDKDGWFWEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLP--------AAVNDHLKNEKKILLKAGSYDDERTVVSVKTDNQEETGPEVDDSKVTYDTIQSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAEYLKLRDDAHLINAETVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISFLGNALVSVSSDQTGAKFDGATSNNTHVSGDDHIDVTNPKDLAKGYYGFVSTDKLAAGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKELPSAKVVITEDANADKKVDWQDGAIAYRSINNPQGWKKVKDITAYRIAN--FGSQAQNPFLTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIEKAKKYGAHLGIHVNASE-TYPESKYFNEKILRKNPDGSYSYGWNWLDQGINID-AAYDLAHGRLARWEDLKKKLGDGLDF-IY-VDVWGNGQSGDNGAWATHVLAKEINKQGWRF--------------------AIEWGHGGEYDSTFHHWAADLTYGGYTNKGI---NS-AITRFIRNHQKDAWVGDYRSYGGAANYP-------------------------------------------------LLGGYSKDFEGWQGRSDY------------NGYVTNLFAHDVTKYFQ-HF--T-VSKWENGTPVTTDNGSTYKWTPER-----VELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGR------VIQDGSAYLTPWNWDANGKKLSTDKEKYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKITLDLLANQPYVLYRSKQTNPESWSEG--H---IY--DQ-GFN--S---------G---TLK-HW---T---I--SGDASKAEIVKSQGANDLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTY-T-------------NKSLALNYVKAYAHNTRRNNATVDDTSYFQNYAFFTTGSDVSNVTLTLSREAGDEATYFDE--------------------------------IRTFENNSSYGDKHDTGKGTFKQDFENV-----AQGIFPFVVGGVEGVEDNRTHLSEKHDPYTQRGWNGKKVDDVIEGNWSLKTN---GLVSRRNLVYQTIPQNFRFEAGKTYRVTFEYEAGSDNTYAFVVGKGEFQSGRRGTQASNLEHELPNTWTDSKKAKKATFLVTGAETGDTWVGIYSTGNASNTRGDSGGNANFRGYNDFDNLQIEEITLTGKLTENALKNYLPTVA-KEAVFNLSQADDDISVEEARAEIAKI------------------------------  ----------------------------------------------------------------------------------------------------------------------------------------------------------MGVRNSVLS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GKKADE------------------------------LEK---IR--L-RPNGKK-----------------------------KYMLKHVVWAANELDRFGLAESLLENKE--GCQKILSVLAPLVPTGSENLKSL----------YN-TVCV----------------------------IWCIHAEEKVKHTEEAKQIVQRHLV-V------ETGTTETMPKTSRPTA--PSSGRGG---------------------------NYPVQQIGGNYVHLPLSPRTLNAWVKLIEEKKFGAEVVPGFQALSEGCTP-----------------YDINQMLNCVGDHQAAMQIIRDIINEEAADWDLQHPQPA---------------------PQQGQLREPS---GSDIAGTTSS--------VDEQIQWMYRQQNPIPVGN--IYRRWI---------------QLGL--------------------QKCVRMYN-PT-----------------------------NILDVKQGPKEPFQSYVDRFYKSLRAEQTDAAVKNWMTQTLLIQNANPDCKLVLKGLGVNPTL-----------------EEMLTACQ-------------------------GVGG----PGQKARLMAEALKEALAPVPIPFAAAQQRG----PRKPIK-----------------------------------------CWNCGKEGHSARQCRAPRRQGCWKCGKMDHVMAKCP------------------------DRQAGFLGLG-PWGKKPRNFPMAQ---------------------------------VHQGLMP-----------------------------TA----------------------------PPED-PAV--------------------------------------------------------------------DLLKNYMQLGKQQ--------------------REKQRESREKPYKEVTEDLLHLNSLFGGDQ    ------------------------------------------------------------------------------------------------------------------------------------------------VHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWM-TNNPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEPF-RDYVDRFYKTLR--------AEQASQEVKN-WMTETLLVQNANPDCKTILKALGP-----------------AATLEEMMTACQG----------------------------------------------------------------------------------------------------------------------------------------------------------  MGVRNSVLSGKKADELEKIRLRPNGKKKYMLKHVVWAANELDRFGLAESLLENKEGCQKILSVLAPLVPTGSENLKSLYNTVCVIWCIHAEEKVKHTEEAKQIVQRHLVVETGTTETMPKTSRPTAPSSGRGGNYPVQQIGGNYVHLPLSPRTLNAWVKLIEEKKFGAEVVPGFQALSEGCTPYDINQMLNCVGDHQAAMQIIRDIINEEAADWDLQHPQP--APQQGQLREPSGSDIAGTTSSVDEQIQWMYRQQNPIPVGNIYRRWIQLGLQKCVRMYNPTNILDVKQGP-KEPFQSYVDRFYKSLRAEQTDAAVKNWMT--QT-LLI--QNANPDC-------------KLVLKGLGVNPTLEEMLTACQGV-GG----PGQKARLMAEALKEALAPVPIPFAAAQQRGPRKPIKCWNCGKEGHSARQCRAPRRQGCWKCGKMDHVMAKCPDRQAGFLGLGPWGKKPRNFPMAQVHQGLMPTAPPEDPAVDLLKNYMQLGKQQREKQRESREKPYKEVTEDLLHLNSLFGGDQ    SDSQQSIKVLEELFQKLSVATADNRHEIASEVASFLNGNIIEHDVPEHFFGELAKGIKDKKTAANAQAVAHIANQSNLSPSVEPYIVQLVPAICTNAGNKDKEIQSVASETLISIVNAVNPVAIKALLPHLTNAIVETNKWQEKIAILAAFSAVDAAKDQVALRPELIPVLSETWDTKKEVKAAATAATKATETVDN--KDIE------RFIPSLIQCIADPTEVPETVHLLGATTFVAE------VTPATLSIVPLLSRGLNE-RETGIKRKSAVIID--------------N--CKLVEDPQVIAPFLGKLLPGLKSNFATIADPEAREVTLRALKTLRRVGNVGED-DAI--PELSHAGDVSTTLQVVNELLKDETVAPRFKIVVEYIAAIGADLIDERIIDQQAWFTHITPYTIFLHEKKAKDILDEFRKRAVDNIPVGPNFDDEEDEGEDLCNCE-----------------------------------------------------------F-SLAYGAKILLNKTQL-RLKRARRYGICGPNGCGKSTLRAIA----------------------------------------------------------------NGQVDGFPT-QEECRT--------------V--YVE--------HDIDGTHSDTSVLDFVFESGVGTKEAIKDKLIEFGFTDE-IAP-ISALSGGWKKLALARAVLRNADILLLDEPTNHLDT--VNV-AWL-VNYLNTCGITSITISH-----------------DSVFLDNV-------CEY--IINYEGLK-----LR-KY------------------------KG-----N--FTEFVKKCPAAKAYEELSNTDLEFKFPEPGYLEGVKTKQKAIVKVTNEFQYPGTSKPQITDINFQCSLSSRIAVIGPNGAGKSTLINVLTGELLPTSGEVYTHENCRIAYIKQHAF--AHIESHLDKTPSEYIQWRFQTGEDRETDRANRQINENDAEANKIFKIEGTPRRIAGIHSRRKFKNTYEYECSFLLGENIGKSERWV-PSVDNAWIPRGELVESHSKVAEVDKEALASGQFRPLTRKEIEEHCSLGLDPEIVSHSRIRGLSGGQKVKLVLAAGTWQRPHLIVLDEPTNYLDRDSLGALSKALKEFEGGVIIITHSAEFTKNLTEEVWAVKDGRTP---------------------  -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MGVRN-SVLSGKKADELEKIRLRPNGKKKYMLKHVVWAANELDRF--------------GLAESLLENKEGCQKILSVLAPLVPTGSENLKSLYNTVCVIWCIHAEEKVKHTEEAKQIVQRH-LVV-------------------------------ETGTTETMPK-TS--RPTAP------------------------------------------------------------------------------------------------------SSGRGGNYPVQQIGGNYVHLPLSPRTLNAWVKLIEEKKFGAEVVPGFQALSEGCTPYDINQMLNCVGDHQAAMQIIRDI-----------------------INEEAADWDLQHPQPAPQQGQLREPSGSDIAGTTSSVDEQIQWMYRQQNPIPVGNIYRRWIQLGLQKCVRMYNPTNILDVKQGPKEPFQSYVDRFYKSLRAEQTDAAVKNWMT---------QTLLIQNANPDCKLV-L--------KGLGVNPTLEEMLTAC-QG---------------------VGGP-GQK-ARL--------------MAEALKEALAPVPIPFAAAQQ----RGPRKPIKCWNCGKE-----GHSARQ-CR-APRRQGCWKCGKMDHVMAKCPDRQAGFLGLGPWGKKPRNFP-M--A--------------------------------------------------------------------------------------------------------QVHQGLMPTA--------P--P-------------EDPAVDLLKNYMQ-----LGK------------------QQREK---------QRESRE------------------------------------------------------------------------------------------------------------------------------------KPYKEVTEDLLHLNSLFGGDQ

(a)	Residue pairs that are structurally aligned by TM-align program are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade.(more about the colors used)
(b)	Ranking of proteins is based on TM-score of the structural alignment between Model1 and the PDB structures in our template library.
(c)	RMSDa is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDENa is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.

Function Prediction

Predicted EC Numbers

Rank	TM-score	RMSDa	IDENa	Cov.	EC-Score	PDB Hit	EC No.
1	0.3204	3.68	0.54	0.37	0.7890	1e6jP	2.7.7.49
2	0.2602	2.27	0.60	0.28	0.6675	1afvA	2.7.7.7
3	0.2551	1.64	0.60	0.26	0.6549	2pwoC	3.1.26.4
4	0.2535	1.66	0.60	0.26	0.6499	2pwmA	3.4.23.16
5	0.2268	1.60	0.67	0.24	0.6295	2gonA	2.7.7.7

(a)	Ranking is based on EC-score, which is a confidence score for the Enzyme Classification (EC) Number prediction.
(b)	RMSDa is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)	IDENa is percentage sequence identity in the structurally aligned region.
(d)	Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)	EC-Score is defined based on the C-score of the structure prediction and similarity of the model with known enzyme structures, as identified using both global and local structural alignment programs. The global similarity score uses TM-score, IDENa,RMSDa and Cov. of the structural alignment by TM-align, while the local match compares the structural and chemical similarity of local spatial motifs in the model with known catalytic site of enzymes. A prediction with a EC-score >1.1 signifies a prediction with high confidence (upto 3 digit numbers of EC) and vice versa (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).

Predicted GO terms

Rank	TMscore	RMSDa	IDENa	Cov.	PDB Hit	Fh-Score	Associated GO Terms
1	0.1998	1.83	0.89	0.21	1ed1A	0.62	GO:0000287 GO:0003677 GO:0003723 GO:0003887 GO:0003964 GO:0004190 GO:0004523 GO:0005198 GO:0008270 GO:0008907 GO:0016893 GO:0043169 GO:0070001 GO:0000003 GO:0006278 GO:0006310 GO:0006508 GO:0009058 GO:0010926 GO:0010927 GO:0015074 GO:0019067 GO:0021700 GO:0022414 GO:0022607 GO:0032196 GO:0034960 GO:0034961 GO:0043283 GO:0043284 GO:0044085 GO:0044249 GO:0046797 GO:0048646 GO:0048856 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0019012 GO:0019028 GO:0043229
2	0.2535	1.78	0.60	0.26	1m9dD	0.56	GO:0003723 GO:0005198 GO:0008270 GO:0043169 GO:0016032 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0019012 GO:0019028 GO:0043229
3	0.1318	2.45	0.63	0.14	2buoA	0.31	GO:0003723 GO:0005198 GO:0008270 GO:0043169 GO:0016032 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0019012 GO:0019028 GO:0043229
4	0.2236	2.41	0.24	0.24	2eiaB	0.25	GO:0003676 GO:0005198 GO:0008270 GO:0043169 GO:0016032 GO:0044419 GO:0019012 GO:0019013 GO:0019028
5	0.2819	7.16	0.10	0.45	2vbiH	0.21	GO:0000287 GO:0004737 GO:0030976 GO:0050662
6	0.2360	3.21	0.16	0.26	2eiaB	0.20	GO:0003676 GO:0005198 GO:0008270 GO:0043169 GO:0016032 GO:0044419 GO:0019012 GO:0019013 GO:0019028
7	0.3081	7.51	0.07	0.51	1t36G	0.20	GO:0004086 GO:0004088 GO:0005515 GO:0005524 GO:0030145 GO:0030554 GO:0043169 GO:0006221 GO:0006525 GO:0006526 GO:0008652 GO:0009058 GO:0009165 GO:0009308 GO:0009309 GO:0009987 GO:0019752 GO:0044249
8	0.3204	7.70	0.06	0.54	2z8yP	0.18	GO:0005506 GO:0016151 GO:0018492 GO:0043169 GO:0043884 GO:0051539 GO:0006084 GO:0009987 GO:0015977
9	0.1296	2.13	0.34	0.14	2eiaB	0.18	GO:0003676 GO:0005198 GO:0008270 GO:0043169 GO:0016032 GO:0044419 GO:0019012 GO:0019013 GO:0019028
10	0.2828	7.30	0.07	0.46	2ipyB	0.18	GO:0003723 GO:0003994 GO:0005506 GO:0043169 GO:0051539 GO:0006084 GO:0006099 GO:0009056 GO:0009987 GO:0044248 GO:0046356 GO:0005622 GO:0005623 GO:0005737

Consensus Prediction of Gene Ontology terms
Molecular Function    Biological Process   Cellular Location GO term GO-Score    GO term GO-Score   GO term GO-Score GO:0005488 0.289    GO:0016032 0.212    GO:0019028 0.229 GO:0043167 0.289    GO:0044237 0.136    GO:0019012 0.229 GO:0046872 0.289    GO:0008152 0.136    GO:0044423 0.229 GO:0043169 0.268    GO:0009987 0.136    GO:0005737 0.201 GO:0046914 0.268    GO:0044238 0.100    GO:0044424 0.201 GO:0003676 0.229    GO:0009058 0.082    GO:0005623 0.201 GO:0005198 0.212    GO:0006139 0.082    GO:0044464 0.201 GO:0008270 0.212    GO:0044249 0.082    GO:0005622 0.201 GO:0003723 0.167    GO:0044085 0.080    GO:0043227 0.183 GO:0003824 0.140    GO:0048869 0.080    GO:0043229 0.183

(a)	Ranking in the first table is based on a function prediction score (Fh-score), which is calculated based on the C-score of the structure prediction and the TM-score, IDENa, RMSDa and Cov. of the structural alignment by TM-align between the predicted model and the PDB structures (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).
(b)	RMSDa is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)	IDENa is the percentage sequence identity in the structurally aligned region.
(d)	Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)	A consensus prediction of GO terms is derived from the structural analogs that have an Fh-score of >=1.0. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on the Fh-score of the template from which the GO term is derived. A prediction with a GO-score >0.5 signifies a prediction with high confidence and vice versa.

Predicted Binding Site

Binding site residues in the model: PHE: 166    GLY: 167    ALA: 168    GLN: 272    LYS: 273    CYS: 274    VAL: 275    ARG: 276    MET: 277    TYR: 278    ASN: 279    PRO: 280    ASN: 282

Identified analogs with similar binding site: Rank PDB Hit TM-score RMSDa IDENa Cov. BS-score Download Complex Binding site residues on the predicted model 1 3dt7A 0.2614 7.15 0.05 0.42 0.47 Download 166,167,168,272,273,274,275,276,277,278,279,280,282 2 2h3iA 0.2162 3.22 0.43 0.24 0.38 Download 5,6,7,8,16,31,33,34,35,37,38,48,49,51,52,57,81,82,85 3 2k4hA 0.2200 2.54 0.65 0.24 0.34 Download 6,7,8,13,16,30,31,34,35,38,48,51,52,85 4 2q5qB 0.2650 6.91 0.06 0.41 0.24 Download 402,406,408,409,410,411,412,413,414,415,416,417,418,419,429,430 5 3dt7A 0.2614 7.15 0.05 0.42 0.22 Download 276,277,279,282,283,284,285,401,403,419,421,423 6 2ji6A 0.2770 7.67 0.06 0.47 0.20 Download 402,403,406,408,409,410,411,412,413,417,418,419,421,423,430 7 2j3lB 0.2709 7.41 0.04 0.44 0.17 Download 92,101,102,103,104,105,106,107,108,109,111,112,113,114 8 3fsjX 0.2726 7.38 0.05 0.44 0.17 Download 403,405,406,407,408,409,410,411,412,413,414,415,416,417,418,419,421,423,429,430,431,432 9 2ji6A 0.2770 7.67 0.06 0.47 0.16 Download 166,167,168,171,203,206,210,269,270,271,272,273,274,275,276,277,278,279,280,404,406,407,408,409,410,419 10 2ji6A 0.2770 7.67 0.06 0.47 0.16 Download 123,201,202,203,204,205,206,207,208,209,234 (a) Ranking of the analogs in the table is based on their BS-score. BS-score is calculated by first finding the structural analogs of the predicted model based on their TM-score, IDENa and Cov. of the structural alignment. The ligand(s) in the analog structure are then transferred onto the model and the fitness of the ligand-model complex (BS-score) is calculated by comparing the local structure and sequence similarity in the binding site region. (For detail, see Kucukural, Szilagyi, Ambrish, and Zhang, Template based ligand binding site prediction on modeled protein structure, 2009, in preparation). (b) A BS-score of >0.5 signifies a binding site prediction with high confidence and vice-versa. (c) RMSDa the RMSD between residues that are structurally aligned by TM-align. (d) IDENa is the percentage sequence identity in the structurally aligned region. (e) Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model. (f) The image shows the ligand-protein complex with the best BS-score. The ligand is depicted in magenta colored ball & stick, the predicted binding site residues interacting with the ligand are shown as transparent green spheres, while the N & C terminus in the model are marked by blue and red spheres respectively.