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I-TASSER results for job id S34347

  Submitted Sequence

>AFP_Doman3
KYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCC
QLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYV
PPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKC
CQGQEQEVCFAEEGQKLISKTRAALGV

  Predicted Secondary Structure

Sequence                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200
                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |        
KYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV
PredictionCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHCC
Conf.Score911298999999899999978999999999999765578988999999999999999985885336676401237899999871289999995532152578314658999678899899998773207798888748566499999999999997089988999999999999999998376982221240115799999998559

  Top 5 Models predicted by I-TASSER

Download Model 1 Download Model 2
C-score= 1.76
 C-score=-5
 
Estimated accuracy of Model1: 0.96±0.05 (TM-score)    2.0±1.6Å (RMSD)    (Read more about C-score of generated models)

  Top 10 templates used by I-TASSER

RankPDB
Hit		Iden1Iden2Cov.Norm.
Z-score		Download
Align.		                   20                  40                  60                  80                 100                 120                 140                 160                 180                 200
                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |       
Sec.Str
Seq		CCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHCC
KYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV
11gniA 0.47 0.47 1.00 2.49Download PLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALG-
21n5uA 0.47 0.47 1.00 7.11Download PLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALG-
31gniA 0.48 0.45 0.95 4.70Download ----------KQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALG-
41gniA 0.48 0.45 0.95 3.44Download K----------QNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALG-
51gniA 0.48 0.45 0.95 4.28Download ----------KQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALG-
61n5uA 0.47 0.47 1.00 5.93Download PLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALG-
71bj5A 0.44 0.45 0.93 3.97Download -----------QNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFA---EEGKKLVAASQAA
81n5uA 0.47 0.47 1.00 7.35Download PLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALG-
91gniA 0.48 0.45 0.95 4.19Download ----------KQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALG-
101gniA 0.48 0.45 0.95 2.41Download ----------KQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALG-
(a)All the residues are colored in black; however, those residues in template which are identical to the residue in the query sequence are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade. (more about the colors used)
(b)Rank of templates represents the top ten threading templates used by I-TASSER.
(c)Ident1 is the percentage sequence identity of the templates in the threading aligned region with the query sequence.
(d)Ident2 is the percentage sequence identity of the whole template chains with query sequence.
(e)Cov. represents the coverage of the threading alignment and is equal to the number of aligned residues divided by the length of query protein.
(f)Norm. Z-score is the normalized Z-score of the threading alignments. Alignment with a Normalized Z-score >1 mean a good alignment and vice versa.
(g)Download Align. provides the 3D structure of the aligned regions of the threading templates.
(h)The top 10 alignments reported above (in order of their ranking) are from the following threading programs:
       1: MUSTER   2: HHSEARCH   3: SP3   4: PROSPECT2   5: PPA-I   6: HHSEARCH I   7: FUGUE   8: HHSEARCH II   9: SPARKS   10: MUSTER   

  10 proteins in PDB which are structurally closest to the first I-TASSER model (identified by TM-align)

RankTM-scoreRMSDaIDENaCov.PDB
Hit
10.9895 0.41 0.47 1.001gniA
Model1
 
20.7815 2.60 0.15 0.941kxpD
Model1
 
30.4673 5.49 0.10 0.801st6A
Model1
 
40.4599 5.24 0.08 0.773e0sA
Model1
 
50.4551 5.36 0.06 0.771uwkA
Model1
 
60.4545 5.05 0.04 0.722fknA
Model1
 
70.4537 4.61 0.02 0.692hp0A
Model1
 
80.4492 5.41 0.07 0.742fflA
Model1
 
90.4477 4.33 0.05 0.621e9xA
Model1
 
100.4474 5.27 0.05 0.741gpuA
Model1
 
Structural alignment using TM-align
 
HKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALG- 
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV 
 
LERGRDYEKNKVCKEFSHLGKEDFTSLSLVLYSRKFPSGTFEQVSQLVKEVVSLTEACCAEGADPDCYDTRTSALSAKSCESNSPFPVHPLKHQPQEFPTYVEPTNDEICEAFRKDPKEYANQFMWEYSTNYGQAPLSLLVSYTKSYLSMVGSCCTSASPTVCFLKERLQLKHLSLLTTLSNRVCSQYAAYGEKKSRLSNLIKLAQKVPTADLEDVLPLAEDITNILSKCCESASEDCMAKELPEHTVKLCDNL--S---TKNSKFEDCCQEKTAMDVFVCTYFMPAA-QLPELPDVE--LPTNKDVCDPG---NTKVMDKYTFELSRRTHLPEVFLSKVLEPTLKSLGE-CCDVEDSTTCFNAKGPLLKKELSSFIDKGQELCADYSENTFTEYKKKLAERLKAKLPDATPKELAKLVNKRSDFASNCCSINSPPLYCDSEIDAELKNI 
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------K---Y-IQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYA-NRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV----------------------------------------------------------------------- 
 
VPRMPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVSAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVRAAQMLQADPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNTKSQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALALIDSKMNQAKGWLRDPNAPPGDAGEQAIRQILDEAGKAGELCAGKERREILGTCKTLGQMTDQLADLRARGQGATPMAMQKAQQVSQGLDLLTAKVENAARKLEAMTNSKQAIAKKIDAAQNWLADPNGGSEGEEHIRGIMSEARKVAELCEEPKERDDILRSLGEISALTAKLSDLRRHGKGDSPEARALAKQIATSLQNLQSKTNRAVANTRPVKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGRRLANVMMGPYRQDLLAKCDRVDQLAAQLADLAARGEGESPQARAIAAQLQDSLKDLKARMQEAM-----------------------------TQEVSDVFSD-TTTPIKLLAVAATAPSDTPNREEVFEER-----------AANFENHAARLGATA-EKAAAVGTANKTTVEGIQATVKSARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANMQPQ-MLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDP-GLQ-KSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEHLHLTPPPPEEKDEEFPEQKAGEAINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWY 
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K--YIQESQ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQL-------------SEDKLLACGEGAA-DIIIGHLCIRHEMTPVN---------P-GVGQCCTSS-YAN----------------------------RR-PCFSSLVV--DE--TYV-PPA--FS--DDKFI-FHKDLC-QAQGVALQTMKQEFLINLVKQK-PQI---------------T--EEQLEAVIADFSGLL-EKCCQGQEQEVCFAEEGQKLISKT-RAALGV----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 
 
LQLDAHASIHENVRRLLQFT-------TSIMEANEEGIRKDIDSEFLHDFR-VAIRRSRSILRL-LNGVFDPEK--TAW-MLAGLRELGKRTNDLRDSDVYLLRREEYTSLLPPSLRPALDPFFSDLEADKRLHHRQFCR-YLTGREYSG-FM---------------------------T-SLKEFIAEGELPDPETAPLAAEPTGDVAAKTIRKALKKVLVHGRS--DAELHELRIDCKKLRY-LL--EFFASLFPPKATAQVLRQMKTLQDNLGTFVDLTVQMEFLQSRLETISEAAAIGGLLTTLYRKREKVREHFHEIFSGFDSNETGELFDELLTGL 
--------------------KYIQESQAL-AKRSC-G-------LFQKLGEYYLQNAFLVAYTKKAPQLT----SSEL-MAIT-RKMAATAAT-C---------------------------------------C--QLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLV-V--------DETY--VP-PA--FS-DDKFI-FHKDLCQAQGVALQTMKQEFLINLVKQKPQITEE-Q-LEA-VIADFSGLLEKC--C------------------------------------QGQEQEVCFAEEGQ-KLISKTRAALGV- 
 
NNKYRDVEIRAPRGNKLTAKSWLTEAPLRMLMNNLDPQVAENPKELVVYGGIGRAARNWECYDKIVETLTRLEDDETLLVQSGKPVGVFKTHSNAPRVLIANSNLVPH----------WANWEHFNELDAKGLAMYGQMTAGSWIYIGSQGIVQGTY----------------ETFVEAGRQHYGGSLKGKWVLTAGLGG-MGGAQPLAATLAGACSLNIESQQSRIDFRLETRYVDEQATDLDDALVRIAKYTAEGKAISIALHGNAAEILPELVKRGVRPDMVTDQTSAHDPLNGYLPAGWTWEQYRDRAQTEPAAVVKAAKQSMAVH-VQAMLDFQKQGVPTFDYGNNI-----RQMAKE------EGVADAF-DFPGFV--PAYIRPLFCRG-VGPFRWAALSGEAEDIYKTDAKVKELIPDDAHLHRWLDMARERISFQGLPA-RICWVGLGLRAKLGLAFNEMVRSGELSAPVVIG--RDHLDSGSVSSPNAETEAMRDGSDAVSDWPLLNALLNTAGGATWVSLHHGGGVGMGFSQHSGMV-IV-CDGTDEAAERIARVLTNDPGTGVMRHADAGYDIAIDCAKEQGLDLPMITG 
------------------------------------------------------------------------------------------------------------KYIQESQALAKRSCGLFQ-------KLGEYY------------------LQNAFLVAYTKKAPQLTSS------------------E-----LMA-----------------------------------------------------------------------------------ITRKMAATAATC-------------CQ-LSEDKLLACGEGAADIIIGHLCIRH--EMTPVN-----PGVGQC-----CTSSYANRRPCFSSLVVD--ETYV--PPA-FSDDKFIFHKDLC------Q-----------------------------AQG---VAL-------------------------------QTMKQEF---L-I--NLVKQKP--QITEEQLEAVIAD-F------SG----------------LLEKCCQGQEQ--EV-CFAEEGQKLISKTRAAL-G------------------V--- 
 
SIRANRGTELECLGWEQEAVLRMLRNNLDPEVAEKPEDLIVYGGIGKAARDWDAFHAIEHSLKTLKNDETLLVQSGKPVGMFRTHPQAPRVLLANSVLVPKW-----------ADWEHFHELEKKGLMMYGQMTAGSWIYIGSQGILQGTY----------------ETFAELARQHFGGSLKGTLTLTAGLGG-MGGAQPLSVTMNEGVVIAVEVDEKRIDKRIETKYCDRKTASIEEALAWAEEAKLAGKPLSIALLGNAAEVHHTLLNRGVKIDIVTDQTSAHDPLIGYVPEGYSLDEADRLRQDTPELYVRLAKQSMKKH-VEAMLAFQQKGSIVFDYGNNI-----RQV----AKDEGLENAFDF-PGFV--PAYIRPLFCEG-KGPFRWAALSGDPADIYRTDALLKELFPTNKALHRWIDMAQEKVTFQGLPS-RICWLGYGERKKMGLAINELVRTGELKAPVVIG--RDHLDCGSVASPNRETEAMKDGSDAVGDWAVLNALVNTAAGASWVSFHHGGGVGMGYSLHAGMV-AV-ADGSELADERLARVLTSDPGMGIIRHADAGYERAVEVAKEQDIIVPM 
------------------------------------------------------------------------------------------------------KYIQESQALAKRSCGLFQ-------KLGEYY------------------LQNAFLVAYTKKAPQLTSS------------------E-----LMA-----------------------------------------------------------------------------------ITRKMAATAATC-------------CQ-LSEDKLLACGEGAADIIIGHLCIRH--EMTPVN-----PGVGQC--CTSSYANRRPCFSSL-VVD--ETYV--PP-AFSDDKFIFHKDLC-Q----------------------------------AQG---VAL-------------------------------QTMKQEF---L-I--NLVKQK--PQITEEQLEAVIAD-F------SG----------------LLEKCCQGQEQ--EV-CFAEEGQKLISKTRAAL-GV------------------ 
 
HFTTKLAEKVVSAWKAKISQPALKAAQDGVIDTVAAALGGVTEHSVQVALKYVAATGGSGDSKLWGVNQRSNFDAAFVNGAAHAI-DFDDS-FPV-------R--GH-PSSSLVPAIFAVGEHVGANGHNCLKSYVLGIEVVATLGRAVGKGHYLAGWHPTSTLGVFGATTAAALLLGADEEQLRNAWGIAASNSCGIIKNFGTTKPHTGSAARNGVLSAWLSQSFTGCQTVFDDAEGILAYGAQPGPELF-------NAQKFGTPWAIIAPGLYKKSWPSCYANHKPLAGLFAIKEHGLTGQDISHVDVGFLPGVEKPLLYD--PRT---------------TE-EAKFS-IEANIGAALLDGEVSLASFEI--E------------------------HL-DRPARAAKKVTRFDPSETTFSGTTGYTDIVVHTADGKIERRIEATPGSLEDPDDAHLERKFKDCTAWPFGESGLLFDRLRSLTADQGIKTVQP 
-------------------KYIQESQALAKRSCGL-F---------------------------QKL----------GEYYLQNAFLVAYTKKA-PQLTSSELMAITRKMAATAATCCQ-L----S-EDKLLACGEGAADIIIGHLCIRHEMTPV------------------------------------------------------------------------------------------------NPGVGQCCTSSY--ANRRP-CFSSLV--------VDE--T------------------YVP----P--AFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQ-I------TEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALG-V------------------------------------------------------------------------------------ 
 
GAMHALGHCCTVVTTRGPSHWLLLLDTHLGTLPGFKVSAGRGLPAAEVYFEAGPRVSLSRTDATIVAVYQSILFQLLGPTFPASWTEIGATMPHNEYTFPRFISNPPQFATLAFLPLLSPTSPLDLRALMVTAQLMCDAKRLSDELSASLHGRMVATPEISWSLYVVLGIDSTQTSLSYFTRANESITYMRYYATAHNIHLRAADLPLVAAVRLDDLKDHQIPAPGSDDLAPKLRFLPPELCLLLPDEFDLIRVQALQFLPEIAKHICDIQNTICALDKSFPDCGRIGGERYFAITAGLRLDQGRGRGLAGWRTPFGPFGVSHTDVFQRLELLGDAVLGFIVTARLLCLFPDASVGTLVELKMELVRNEALNYLVQTLGLPQLAEFSNNLKSKTWAD-MY----E--EIVGSIFT-G--P---NGIYGCEEFLAKTLMSPEHSKTACPDAVTKASKRVCMGEAGAHEFRSLVDYACEQGISVFCSSRVSTMFLERLRDIPAEDMLDWYRLGIQ---------FSHRSGLSGPGGVVSVIDIMTHLARGLWLGSPGFYVEQPPTIPVLYIYHRS------VQCPVLYGSLTTGPVASKVLALYEKILAYESSGGSKHIAAQTVSRSL----------------------------------------AVPIPSGTIPFLIRLLQI-----ALTPH-VYQKLELLGDAFLKCSLALHLHALHPTLTEGALTRMRQSAE-TNSVLGRLTKRFPSVVSEVIIESHPKIQPDSKVYGDTFEAILAAILLACGEEAAGAFVREH---VLPQV-V--AD--A 
-----------------------------------------------------------------------------------------------------K-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------YIQESQ-----------------A---------------------------------------------------------------------------L--AKRS------------CGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLS----------------------------------------------------------------------EDKLLACGEGAADII---------------------------------------------IGHLCIRH---------------------------------------------EMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDK----------------FIFHKD----LCQ--AQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCC-----------------------QGQEQEVCF-----------------------AEEGQKLISKTRAALGV 
 
MSAVALPRVSGGHDEHGHLEEFRTDPIGLMQRVRDECGDVGTFQLAGKQVVLLSGSHANEFFFRAGDDDLDQAKA-YPFMTPIFGEGVVFDASPERRKEMLHNAALRGEQMKGHAATIEDQVRRMIADWGEAGEID---------LLDFFAELTIYTSSACLIGKKFRDQL-D--GRFAKLYHELERGTDPLAYVDPYLPIESFRRRDEARNGLVALVADIMNGRIANPPTDKSDRDMLDVLIAVKAETGTPRFSAD-EITGMFISMMFAGHHTSSGTASWTLIELMRHRDAYAAVIDELDELYGDGRS---------------------------------------VSFH--ALRQIPQLENVLKETLRLHPPLIILMRVAKGEFEVQGHRIHEGDLVAASPAISNRIPEDFPDPHDFVPARYEQPRQEDLLNRWTWIPFGAGRHRCVGAAFAIMQIKAIFSVLLREYEFEMAQPPESYRNDHSKMVVQLAQPACVRYRRRT------------------------ 
-------------------------------------------------------------------------K-YIQ-ESQALA---------------------------------------------------KRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAAT-AATCCQLS-E--------DKLLACGEGAADIIIGHLCIRHEMTP---------V---NPGVGQ------CCTSSYANRRPCFSSLV-----------------------------------------VDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLV--KQKP----------------------------------------------------------------------------------------------Q-ITEEQLEAVIADFSGLLEK-----------------------------CCQGQEQEVCFAEEGQKLISKTRAALGV 
 
QFTDIDKLAVSTIRILAVDTVSKANSGHPGAPLGMAPAAHVLWSQMRMNPTNPDWINRDRFVLSNGHAVALLYSMLHLTGYDLSIEDLKQFRQLGSRTPGHPEFELPGVEVTTGPLGQGISNAVGMAMA--QANLAATYNKPGF--TLSDN--YTYVFLGDGCLQEGISSEASSLAGHLKLGNLIA--IY-DDNKITIDGATSISFDEDVAKRYEAYGWEVLYVE--NGNEDLAGIAKAIAQAKLSKDKPTLIKMTTTIGYGSLHAGSHSVHGAPLKADDVKQLKSKFGFNPDKSFVVPQEVYDH----------------------YQK-TILKPGVEANNKWNKLFSEYQKKFPELGAELARRLSGQLPANWESKLPTYTAKDSAVATRKLSETVLEDVYNQLPELIGG-SADLTPSNLTRWKEALDFQPPSSGSGNYSGRYIRYGIREHAMGAIMNGISA-FGANYKPYGGTFLNFVSYAAGAVRLSA-------LSG-H-PV--IW-VATHDSIGVGEDGPTHQPIETLAHFRSLPNIQVW-RPADGNE-VSAAYKNSLESKHTPSIIALSRQNLPQLEGSSIESASKGGYVLQDVANPDIILVATGSEVSLSVEAAKTLAAKNIKARVVSLPDFFTFDKQPLEYRLSVLPDNVPIMSVEVLATTCWGKYAHQSFGIDRFGASGKAPEVFKFFGFTPEGVAERAQKTIAFYKGDKLISPLKKAF---- 
----KYIQESQ-A------------------L--AK--R-----------------------S-C--GL-------------------------------F-----------QKLGEYYLQNAF-LV--AYT------------KKAPQLTSSEL--------------------M----AITRKMAATAATCCQLS----------------------------EDKLLACGEGAADIIIGHLCIR----------------------------------------------------------HEMTPVNPGVGQCCTSSYANRRPC-FSS--LVV--DE-TY--V----------P--P-AF--------------------------------------SDDKFIFHKDLCQ-------------------------------AQG-VALQTMKQEFLINLVKQK----------------------PQITEEQLEAVIADFSGLLEKCCQGQ-EQ-------------------------EVC-----FAE-EGQKLISKTRA----------------------------------------------------------------------------------------------------------------------------------------------------------------ALGV 
 
(a)Residue pairs that are structurally aligned by TM-align program are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade.(more about the colors used)
(b)Ranking of proteins is based on TM-score of the structural alignment between Model1 and the PDB structures in our template library.
(c)RMSDa is the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.

  Function Prediction

  Predicted EC Numbers

RankTM-scoreRMSDaIDENaCov.EC-ScorePDB
Hit		EC No.
10.4138 5.94 0.08 0.740.68932q5qB 4.1.1.74
 
20.3829 5.88 0.11 0.680.68282vdcF 1.4.1.13
 
30.4041 5.06 0.10 0.610.67471tj7A 4.3.2.1
 
40.4118 5.95 0.08 0.720.66441yi1A 2.2.1.6
 
50.4401 5.20 0.06 0.730.66211tkcA 2.2.1.1
 
(a)Ranking is based on EC-score, which is a confidence score for the Enzyme Classification (EC) Number prediction.
(b)RMSDa is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)IDENa is percentage sequence identity in the structurally aligned region.
(d)Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)EC-Score is defined based on the C-score of the structure prediction and similarity of the model with known enzyme structures, as identified using both global and local structural alignment programs. The global similarity score uses TM-score, IDENa,RMSDa and Cov. of the structural alignment by TM-align, while the local match compares the structural and chemical similarity of local spatial motifs in the model with known catalytic site of enzymes. A prediction with a EC-score >1.1 signifies a prediction with high confidence (upto 3 digit numbers of EC) and vice versa (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).

  Predicted GO terms

RankTMscoreRMSDaIDENaCov.PDB
Hit		Fh-ScoreAssociated GO Terms
10.7480 2.59 0.14 0.901ma9A 1.36 GO:0003779 GO:0005496 GO:0005499 GO:0051183 GO:0009987 GO:0042359 GO:0051180 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005813 GO:0015630 GO:0043229 GO:0044444
20.5227 2.12 0.12 0.591ma9A 0.91 GO:0003779 GO:0005496 GO:0005499 GO:0051183 GO:0009987 GO:0042359 GO:0051180 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005813 GO:0015630 GO:0043229 GO:0044444
30.4294 2.56 0.20 0.491ma9A 0.84 GO:0003779 GO:0005496 GO:0005499 GO:0051183 GO:0009987 GO:0042359 GO:0051180 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005813 GO:0015630 GO:0043229 GO:0044444
40.4138 5.94 0.08 0.742q5qB 0.69 GO:0000287 GO:0030976 GO:0047434 GO:0050662
50.4294 5.82 0.08 0.741zcsA 0.69 GO:0005506 GO:0009055 GO:0030151 GO:0033727 GO:0043169 GO:0051537 GO:0055114
60.3829 5.88 0.11 0.682vdcF 0.68 GO:0004355 GO:0005506 GO:0015930 GO:0043169 GO:0051538 GO:0006536 GO:0006537 GO:0006541 GO:0008652 GO:0009058 GO:0009308 GO:0009309 GO:0009987 GO:0019752 GO:0044249 GO:0055114
70.4203 5.81 0.08 0.741z8nA 0.68 GO:0000287 GO:0003984 GO:0030976 GO:0050660 GO:0050662 GO:0008652 GO:0009058 GO:0009082 GO:0009308 GO:0009309 GO:0009635 GO:0009636 GO:0009987 GO:0019752 GO:0044249 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0043229 GO:0044444
80.4275 5.63 0.07 0.752ihvD 0.68 GO:0000287 GO:0016740 GO:0030976 GO:0050662
90.4686 4.68 0.04 0.712hp3B 0.67 GO:0047547 GO:0009056 GO:0009987 GO:0019541 GO:0019543 GO:0044248
100.4555 5.18 0.05 0.732fknD 0.67 GO:0016153 GO:0006548 GO:0009056 GO:0009063 GO:0009077 GO:0009308 GO:0009310 GO:0009987 GO:0019752 GO:0044248 GO:0005622 GO:0005623 GO:0005737

Consensus Prediction of Gene Ontology terms 
Molecular Function  Biological Process Cellular Location
GO termGO-Score  GO termGO-Score GO termGO-Score
GO:00198420.515  GO:00442370.713  GO:00056230.708
GO:00038240.476  GO:00099870.713  GO:00056220.708
GO:00431670.342  GO:00067660.376  GO:00057370.643
GO:00468720.342  GO:00442380.336  GO:00444440.576
GO:00511830.310  GO:00512340.310  GO:00432290.575
GO:00055150.310  GO:00067750.310  GO:00444220.442
GO:00054960.310  GO:00511800.310  GO:00444460.442
GO:00080920.310  GO:00068100.310  GO:00055760.376
GO:00054990.310  GO:00423590.310  GO:00444210.310
GO:00082890.310  GO:00511790.310  GO:00056150.310

(a)Ranking in the first table is based on a function prediction score (Fh-score), which is calculated based on the C-score of the structure prediction and the TM-score, IDENa, RMSDa and Cov. of the structural alignment by TM-align between the predicted model and the PDB structures (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).
(b)RMSDa is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)IDENa is the percentage sequence identity in the structurally aligned region.
(d)Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)A consensus prediction of GO terms is derived from the structural analogs that have an Fh-score of >=1.0. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on the Fh-score of the template from which the GO term is derived. A prediction with a GO-score >0.5 signifies a prediction with high confidence and vice versa.


  Predicted Binding Site


Binding site residues in the model:
LEU:9  SER:13  LEU:16  PHE:29  TYR:33  LYS:36  MET:52  
ILE:79  LEU:82  CYS:98  ARG:106  PHE:110  SER:111  
Identified analogs with similar binding site:

RankPDB
Hit		TM-scoreRMSDaIDENaCov.BS-scoreDownload
Complex		Binding site residues on the predicted model
11j78B0.6975 3.55 0.15 0.88 0.55Download9,13,16,29,33,36,52,79,82,98,106,110,111
21yhyA0.3516 5.73 0.11 0.59 0.43Download7,8,9,10,11,12,13,14,15,16,17,18,20,21,22,23,25,26,29,47,49,50,51,52,53,54,55,56,57,58,59,60,61,62,67,140,141,144,148
31bccC0.4015 5.79 0.09 0.66 0.21Download10,11,13,14,15,29,52,56,59,60,63,64,65,66,67,68,69,70,71,72,73,75,107
42fynA0.3923 5.91 0.09 0.65 0.18Download8,9,10,11,12,13,16,31,35,115,116,117,118,159,161,163
52fynA0.3923 5.91 0.09 0.65 0.17Download30,33,34,37,38,39,40,41,42,44,45,46,48,49,52,78,79,82,86,90,91,92,94,95,96,98,99,110,113,152
61bccC0.4015 5.79 0.09 0.66 0.14Download20,23,24,26,27,31,124,144,146,147,148,150,151,154,155,158,161,162,163,164,165,166,167,168,169,170,201,202,203,204,205,206,207
71bccC0.4015 5.79 0.09 0.66 0.09Download36,37,38,39,91,92,93,94,95,97,98,101,109,110,112,113,114,115,116,117,118,119
81yhyA0.3516 5.73 0.11 0.59 0.08Download134,135,136,137,138,139,140,143,181
91fs7A0.3982 5.89 0.07 0.69 0.06Download31,32,33,34,35,36,37,38,92,93,94,95,110,113,114,115,116,117,118,119,120,159,160,161
102fynA0.3923 5.91 0.09 0.65 0.03Download21,22,23,24,25,26,144

(a)Ranking of the analogs in the table is based on their BS-score. BS-score is calculated by first finding the structural analogs of the predicted model based on their TM-score, IDENa and Cov. of the structural alignment. The ligand(s) in the analog structure are then transferred onto the model and the fitness of the ligand-model complex (BS-score) is calculated by comparing the local structure and sequence similarity in the binding site region. (For detail, see Kucukural, Szilagyi, Ambrish, and Zhang, Template based ligand binding site prediction on modeled protein structure, 2009, in preparation).
(b) A BS-score of >0.5 signifies a binding site prediction with high confidence and vice-versa.
(c)RMSDa the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.
(f)The image shows the ligand-protein complex with the best BS-score. The ligand is depicted in magenta colored ball & stick, the predicted binding site residues interacting with the ligand are shown as transparent green spheres, while the N & C terminus in the model are marked by blue and red spheres respectively.



 Please cite following articles when you use the I-TASSER server:
1.Yang Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9:40 (2008).
2.Yang Zhang. Template-based modeling and free modeling by I-TASSER in CASP7. Proteins, 8: 108-117 (2007).
3.Sitao Wu, Jeffrey Skolnick, Yang Zhang. Ab initio modeling of small proteins by iterative TASSER simulations. BMC Biology, 5:17 (2007).