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I-TASSER results for job id S34353

  Submitted Sequence

>your_protein
MKSPHKLLFIFLITTSTVIVLSSSNWLTTIGLEINMLGFIPLIYHKITANESETVVKYLV
PQSLGSTIFIVGALISYHFNSIQILIPIAICLKLGAAPLHLWFPAVISGLSLTPAFTLLT
WQKIAPIFAISSIDPSLIARILPIAGIRALGGIGGLNQTDVRLLLSYSSIAHTGILARIT
APAAVLTLYLTIYILINISLFTALTQNRVKIHKQLLPGSQKPYESFLLAITILSLGGLPP
LTGFAIKLLVLIFTQAKTIILVSLISGAILRLYYYLSLTFSPLLRLHKIPISQFKLTTST
SISFLLSQIMPLSLLF

  Predicted Secondary Structure

Sequence                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300
                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                 
MKSPHKLLFIFLITTSTVIVLSSSNWLTTIGLEINMLGFIPLIYHKITANESETVVKYLVPQSLGSTIFIVGALISYHFNSIQILIPIAICLKLGAAPLHLWFPAVISGLSLTPAFTLLTWQKIAPIFAISSIDPSLIARILPIAGIRALGGIGGLNQTDVRLLLSYSSIAHTGILARITAPAAVLTLYLTIYILINISLFTALTQNRVKIHKQLLPGSQKPYESFLLAITILSLGGLPPLTGFAIKLLVLIFTQAKTIILVSLISGAILRLYYYLSLTFSPLLRLHKIPISQFKLTTSTSISFLLSQIMPLSLLF
PredictionCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
Conf.Score9699999999999987799988769999999999999999998557896299999999999999999999999999789889999999999984862776420898758947899999999988899999997577638999999999833100414999999999989999999999998768999999999999999999999805178898865651599999999999975899986230189999999977419999999999999999999999874517999887765643672588999999999859

  Top 5 Models predicted by I-TASSER

Download Model 1 Download Model 2 Download Model 3 Download Model 4 Download Model 5
C-score=-3.57
 C-score=-4.65
 C-score=-5
 C-score=-4.93
 C-score=-5
 
Estimated accuracy of Model1: 0.32±0.11 (TM-score)    15.1±3.5Å (RMSD)    (Read more about C-score of generated models)

  Top 10 templates used by I-TASSER

RankPDB
Hit		Iden1Iden2Cov.Norm.
Z-score		Download
Align.		                   20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300
                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                
Sec.Str
Seq		CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
MKSPHKLLFIFLITTSTVIVLSSSNWLTTIGLEINMLGFIPLIYHKITANESETVVKYLVPQSLGSTIFIVGALISYHFNSIQILIPIAICLKLGAAPLHLWFPAVISGLSLTPAFTLLTWQKIAPIFAISSIDPSLIARILPIAGIRALGGIGGLNQTDVRLLLSYSSIAHTGILARITAPAAVLTLYLTIYILINISLFTALTQNRVKIHKQLLPGSQKPYESFLLAITILSLGGLPPLTGFAIKLLVLIFTQAKTIILVSLISGAILRLYYYLSLTFSPLLRLHKIPISQFKLTTSTSISFLLSQIMPLSLLF
11fqvA 0.13 0.18 0.86 0.58Download LPDELLLGIFSCLCLPELLKVSCKRWYRLA--------------DESLWQTLDLTGKNL--HPDVTGRLLSQGVIAFRCPRSFMDQPLAEHFSFRVQHMD------LSNSVIEVSTLHGILSQCSKLQNLSLEGLRLSDPIVNTLAKNSLSGCSGFSEFALQTLLSSFDFTEKHVQVAVAHVSETIQLNLSGYKNLQKSDLSTLVRRCPNLVHLDLSDSVMLKNDCFQEFF-LSLSRCYDIIPETLLEL-LKTLQGIVPDGTLQLLKEALP----LQISHFTTIARPTQEIWGIKCRLTLQ---------------
21bb1A 0.18 0.03 0.11 0.52Download ---------------EKIAAIKE----EQAAIEEEI----------QAIKEEIAAIKYLIAQI-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
32v7bA 0.12 0.19 0.79 0.69Download DTVALPVAFLGALYAG--------------------VVPVVA-----NTLLTPADYVYMLTHSHARAVIA--------SGALVQNVTQALE-SAGCQPLAPLFEELIDAAAPAA-----KAAATGCIAFWLYSKPKHTHANLYWTAELYAKPILGIAENDV--VFSAAKLFFAYGLGNGL-TFPLSTAILMAERPTADAIFARLVEHRPTVPTLYANMLVSPNLPARADVAICTSAGEA------------------LPREIGERFTAHFGC--EILDGIGSTEMLHIFLSNR---AGAVEYGTTGRPVPGYEIEL
41ee4A 0.09 0.21 0.99 1.52Download DDMQEQLSATVKFRIDVVIQAGVVPRLVEFMLQLEAAWALTNIAS-GTSAQTKVVVDADAVPLFIQLLVKEQAIWALGNVAGDSTDYRDYVLQCNAMEP---ILGLFNSNKPSLIRTATWWSVVSQALPTLAKLIYSMDTETLVDACWAISYLSDGPQEAIQAVIDVRIPKRLVELLSHESTLVQTPALRAVGNIVTGNDLQTQVVINAGVLPALRLLIKKEACWTISNIAVIDANLIPPLVKLLEVSNASSGGLQRPDIIRYLVEVTLDALENILKMGEADKEARGLNINENADFIEKAGGMEKIFNCKIIETYF
51kgnA 0.08 0.18 0.78 1.12Download IPVSNDIQSWNKMTPQEQLATMRVFTGLTLLDTIQGTVGAISLLPDAETMHEEAVYTNIAFMESVHAKSYSNIFMTLASTPQINEAFRWSEENENLQRKAKIIMSYYNGDDKVASTLLESFLFYSGFYLPMYLS-SRAKLTNTADIIRLI-------IRDESVHGYYIGYKYQQGVKK-LSEAEQEEYKAYTFDLMYDLYENEIEYTEDIYDDLGWTEDVKRFLRYNANKALNNL-GYEGLFP----------TDETKVSPAILSSLS------------------------------------------------
62ip6A 0.07 0.05 0.27 0.59Download ------------------------------------------------EHIKQQALDLFTRLQ----FLLQKHDTIEPYQYVLDILETGIS-------------KTKHNQQTPERQARVVYNKIASQALVDKLHFTAENKVLAAINELA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------
71jfaA 0.11 0.15 0.99 0.50Download MENPTEYFLNTTVRLLEYIRYRDSNY-TREERIENLHYAYNKAAHHFAQPRQQQLLK-VDPKRLQASLQTIVGMVVYSWAKVECMADLSIHYTYTLVLDDSKDDPYPTMVNYFDDLQAGREQAHPWWALVNEHFPNVLRHFGPFCSLNLIRSTLDFFEGCWIEQYNFGSHDYPQFLRRMNGLGHCVGASLITSAIAQMENWMVWVNDLMSFYKEFDERDQISLVKNYVVSLHEALEKLTQDTLHSSKQMVAVFMDTIECFMHGYVTWHLCDRRYRLSEIYEDAQKFCKFYEQAANVGAVSPSEWAYPPVAQLANV-
82akhA 0.12 0.20 0.76 0.30Download -------ISIFALGIMPYISAS-------IIIQLLTV-VHPTLAEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATNPGFAFYFTAVVSLVTG-TMFLMWLGEQITGISIIIFAGIVAPAIAHTIEQARQGDLHFLVLLLVAVLVFATFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSLPLKVNMAGVIPAIFASSIILFPATIASWFGGGTG--------------------------WNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTALVFNP----------------------------------
91okcA 0.10 0.18 0.84 0.33Download ALSFLKDFLAGGVAAAISKTAVAPIERVKLLLQVQHA-SKQISAEKQYKGIIDCVVRIPKEQG-------FLSFWRGN-LANVIRYFPTQALNFAF--KDKYKQIFLGGVDRHKQFWRYFAGNLASGGAAGATSLCVYPLDFARTRLAADVGKGAAQGNCITKIFKSDGLRGLGFNVSVQGIIIYRAAYFGVYDTAKGPDPIIVSWMIAQTVTAVAGLVSYPFDTVRRRMMMQSGRADIMYTGTVDCWRKIAKDEGPKWSNVLRGMGGAFVLVLYDEI--------------------------------------
102qfzA 0.14 0.16 0.86 0.57Download ----SRLDKFKQLLAGPNTDLEELRRLSWSG--------IP---KPVRPMTWKLLSGYLRPATLQRKQKEYFAFIEHYHQDTYRQIHIDIP----MSPEALILQPKVTEI-FERILFIWAIR---P-GINDLV-----TPFFVVFICEYIETVDVVPAEVLCNIEADTYWCMSKLLDGIQDNQPGIQMKVKMLEELVSRID-------EQVHRHLDQHEVRYLQFAFRWMNNLLMREVPRCT--IRLWDTYQSEPDGFSHFHLYVCAAFLVRWRKEILEFQELLLLPTAHWDDED------ISLLLAEAYRLKFAF
(a)All the residues are colored in black; however, those residues in template which are identical to the residue in the query sequence are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade. (more about the colors used)
(b)Rank of templates represents the top ten threading templates used by I-TASSER.
(c)Ident1 is the percentage sequence identity of the templates in the threading aligned region with the query sequence.
(d)Ident2 is the percentage sequence identity of the whole template chains with query sequence.
(e)Cov. represents the coverage of the threading alignment and is equal to the number of aligned residues divided by the length of query protein.
(f)Norm. Z-score is the normalized Z-score of the threading alignments. Alignment with a Normalized Z-score >1 mean a good alignment and vice versa.
(g)Download Align. provides the 3D structure of the aligned regions of the threading templates.
(h)The top 10 alignments reported above (in order of their ranking) are from the following threading programs:
       1: MUSTER   2: HHSEARCH   3: SP3   4: PROSPECT2   5: PPA-I   6: HHSEARCH I   7: FUGUE   8: HHSEARCH II   9: SPARKS   10: MUSTER   

  10 proteins in PDB which are structurally closest to the first I-TASSER model (identified by TM-align)

RankTM-scoreRMSDaIDENaCov.PDB
Hit
10.7495 3.09 0.09 0.863dh4A
Model1
 
20.6276 4.44 0.07 0.852a65A
Model1
 
30.5987 4.43 0.12 0.812w8aA
Model1
 
40.5939 4.22 0.10 0.782jlnA
Model1
 
50.4034 6.22 0.04 0.652et6A
Model1
 
60.4024 6.23 0.06 0.641no3A
Model1
 
70.4023 6.07 0.02 0.641y79A
Model1
 
80.4009 5.83 0.07 0.632f5uA
Model1
 
90.3877 6.29 0.05 0.642ivfA
Model1
 
100.3849 6.60 0.05 0.651loxA
Model1
 
Structural alignment using TM-align
 
GGGGGGGGGGGGGGGGGAGKSLPWWAVGASLIAANISAEQFIGMSGSGYSIGLAIASYEWMSAITLIIVGKYFLPIFIEKGIYTIPEFVEKRFNKKLKTILAVFWISLYIFVNLTSVLYLGGLALETILG---------I---PLMYSILGLALFALV-YSI-V-VWTDVIQVFFLVLGGFMTTYMAVSFIGGTDGWFAGVSKMVDAAPGHFEMILDQSNPQYMNLPGIAVLIGGLWVANLYYWGFNQYIIQRTLAA-------------KSVSEAQKGIVFAAFLKLIVPFLVVLPGIAAYVITSDPQLMASLGDIAATNL-PSAANADKAYPWLTQFL------------P--VGVKGVVFAALAAAIVSSLASMLNSTATIFTMDIYKEYISPDSGDHKLVNVGRTAAVVALIIACLIAPMLGGIGQAFQYIQEYTGLVSPGILAVFLLGLFWKKTTSKGAIIGVVASIPFALFLKFMPLSMPFMDQMLYTLLFTMVVIAFTSLSTSINDDDPKGISVTSSMFVTDRSFNIAAYGIMIVLAVLYTLFWVLYK 
----------------------M-KSPHKLLFIFLITTSTVIVLSSSN---WLTTIGLEINMLGFIPLIYHKIT--AN---ESETVVKYLV-PQ-SLGSTIFIVGALISYHFNSIQILIPIAICLKLGAAPLHLWFPAVISGLSLTPAFTLLTWQKIAPIFAISSIDPSLIARILPIAGIRA-LGG----------IG-----------------------------------------------------------GLNQTDVRLLLSYSSIAHTGILARITAPAAVLTLYLTIYILINISLFTALTQNRVKIHKQLLPGSQKP-YESFLLAITILSLGGLPPLTGFAIKLLVLIFTQAKTIILVSLISGAILRLYYYLSLTFSPLL-RLH---KIPISQFKLTTSTSISFLLSQIMPLSLLF----------------------------------------------------------------------------------------------------------------------------------- 
 
REHWATRLGLILA---------MAGNAVGLGNFLRFPVQAAENGGGAFMIPYIIAFLLVGIPLMWIEWAMGRYGGAQGHGTTPAIFYLLWRNRFAKILGVFGLWIPLVVAIYYVYIESWTLGFAIKFLVGLVPEPPPTDPDSILRPFKEFLYSYIGVPKGDEPILKPSLFAYIVFLITMFINVSILIRGISKGIERFAKIAMPTLFILAVFLVI-RVFLLETPNGTAADGLNFLWT-PD-FEKLKDPGVWIAAVGQIFFTLSLGFGAIITYASYVRKDQ--------------DIVLSGLTAAT-LNE--KAEVILGGSISIPAAVAFFGVANAVAIAKAGAFNLGFITLPAIFSQ----------------------------------TAGGTFLGFLWFFLLFFAGLTSSIAIMQPM-I------A--FLEDELKL-------------S--RKHAVLWTAAIVFFSAHLVMFLNKSLDEMDFWAGTIGVVFFGLTELIIFFWIFGADKAWEEINRGGIIKVPR------------------------------------------------------------IYY--YVMRYITPAFLAVLLVVWAREYIPKIMEETHWTVWITRFYIIGLFLFLTFLVFLAERRRNHESAGT 
-------------MKSPHKLLFIFLITTSTV--------------------------------------------------------------------------I-VLSSSNWLTTIGLEINM--L-------------------GF------------------------------------------------------------I--P--LIY--HKI-TANESETVVKYLVPQSLGSTIFIVGALISYHFNSIQILIPIAICLKLGAAPLHLWFPAVISGLSLTPAFTLLTWQKIAPIFAIS-SIDP--------------------------------------------SLIARILPIAGIRALGGIGGLNQTDVRLLLSYSSIAHTGILARITAPAAVLTLYLTIYILINISLFTALTQNRVKIHKQLLPGSQKPYESFLLAITILSLGGLPP------------------------------------------------------------------LTGFAIKLLVLIFTQAKTIILVSLISGAILRLYYYLSLTFSPLLRLHKIPISQFKLTTSTS--ISFLL-SQIMPLSL-LF--------------------------------------------------- 
 
SLNWSVIVPALVIVLATVVWGIGFKDSFTNFASSALSAVVDNLGWAFILFGTVFVFFIVVIAASKFGTIRLGRIDEAPEFRTVSWIS--FA-AGGIGLFYGTTEPLTFYRNGVPGHDEHNVGVASTTFHWTLHPWAIYAIVG-LAIAYSTFRVGRKQLLSSAFVPLIGEKGAEGWLGKLIDILAIIATVFGTA-CSLGLGALQIGAGLSAAN---I--IESDW--TIVGIVSVLTLAFIFSAISGVGKGIQYLSNANVLAALLAIFVFVVGPTVSILNLLPGSIGNYLSNFFQAGRTASADGTAGEWLGSWTIFYWAWWISWSPFVGFLARISR--------------GRSIREFILGVLLVPAGVSTVWFSIFGGTAIVFEQNG-ES--I---W--GD--GAA-EEQLFGLL--HAL----------PG-GQIGII-AILLGTFFITSADSASTV-GT-SQHG-QL-----EA-NKWVTAAWGVATAAIGLTLLLSGGDNALSNLQNVTIVAATPFLFVVIGLFALVKDLSNDVIYLEYREQQRFNARLARERRVHNEHRKRELAAKRRRERKAS 
--------------------------------------------------------------------------------M-KSPHKLLFIFLITTSTVIVLSS--SN---------------WLTTIGLEINMLGFIPLIYHKITANESE-T-VV----KY-L-----VPQS--LGSTIFIVGALISYHFNSIQILIPIAICLKLGAAPLHLWFPAVISGLSLTPAFTLLTWQKIAPI-FA---ISSI-DPSLIARILPIAGIRALGG---I---GG-L------------------------------------------------------NQTDVRLLLSYSSIAHT-GILARIT-APAAVLTLYLTIYILINISLFTALTQNRVKIHKQLLPGSQKPYESFLLAITILSLGGLPPLTGFAIKLLVLIFTQAKTIILVSLISGAILRLYYYLSLTFSPLLRL-HKIPISQFKLTTSTSISFLLSQIMPLSLLF------------------------------------------------------------------------------- 
 
EARSLLNPSNAPTRYAERSVGPFSLAAIWFAM-AIQVAIFIAAGQMTSSFQVWQVIVAIAAGCTIAVILLF-FTQSAAIRWGINFTVAARMPFGIRGSLIPITLKALLSLF-WFGFQTWLGAL-AL-DE-ITRLLTGFT--N-----L-PLWIVIFGAIQVVTTFYGITFIRWMNVFASPVLLAMGVYMVYLMLDGADVSLGEVMSMGGENPGMPFSTAIMIFVGGWIAVVVSIHDIVKECKVDPNASRE---------------GQTKADARYATAQWLGMVPASIIFGFIGAASMVLVG---------------------EW-NPVIAI-T-EVVGG--------VS-IPMAILFQVFVLLATWSTNPAANLLSPAYTLCSTF-P-RV---FTFKTGVIVSAVVGLLM-MPWQFAGVLNTFLNLLASALGPLAGIMISDYFLVRRRRISLHDLYRTKGIYTYWRGVNWVALAVYAVALAVSFLTPDLMFVTGLIAALLLHIPAMRWVAKTFPLFSEAESRNEDYLRPIG 
---------------------MKSPHKLLFIFLITTSTVIVLSSSNW-LT----T-IGLEINMLGFIPLIYHKITANES-E-T-V-VKYLVPQS--LGSTIFIVGALISYHFNSIQILIPIAICLKLGAAPLHLWFPAVISGLSLTPAFTLLTWQKIAPIFAIS-S--ID--PS-LIARILPIAGIRALGG--------IG-------------------------------------------------GLNQTDVRLLLSYSSI--AHTGILARITAPAAVLTLYLTIYILINISLFTALTQNRVKIHKQLLPGSQKPYESFLLAITILSLGGLPP-LTGFAIKLLVLIFTQAKTIILVSLISGAILRL-YYYLSLTFSPLLRLHKIPISQFKLTTSTSISFLLSQIMPLSLLF------------------------------------------------------------------------------------------------------------------- 
 
SPVDFKDKVVIITGAGGGLGKYYSLEFAKLGAKVVVNDLKAADVVVDEIVKNGGVAVADYNNVLDGDKIVETAVKNFGTVHVIINNAGILRD----------------------ASMKKMTEKDYKLVIDVHLNGAFAVTKAAWPYFQKQKYGRIVNTSSPAGLYGNFGQANYASAKSALLGFAETLAKEGAKYNIKANAIAPLARSRMTESIMPPPMLEKL-----GPEKVAPLVLYLSSAENELTGQFFEVAAG-FYAQ---IRWERSGGVLFKPDQSFTA---------------EVVAKRF--S-----------EIL-DYDDSRKPEYLKNQYPFMLNDYATLTNEARKLPANDASGAPTVSLKDKVVLITG---AGAGLGKEYAKWFAKYGAKVVVNDFKDATKTVDEIKAAGGEAWPDQHDVAKDSEAIIKNVIDKYGTIDILVNNAGIL-RD--RSFAKMSKQEWDSVQQVHLIGTFNLSRLAWPYFVEKQFGRIINITSTSGIYGNFGQANYSSSKAGILGLSKTMAI------------------EGAKNNIKVNIVAPHAETAMKNLYHADQV-------------------APLLVYLGTDDVPVTGETFEIGGGWIGNTRWQ--RA-KGAVSHDEHTTVEFIKEHLN---EIT-DFTTDTENPKSTTESSMAILSAVGG-- 
--------------------------------------------------------------------------------------------MKSPHKLLFIFLITTSTVIVLSSSNWLTTIGLEINMLGF-IPLIYHKIT-AN--E-----SETV----------------------------------------VKY-----------------------LVPQSLGSTIFIV----------------GALI-SY---HFNS------------------IQILIPIAICLKLGAAPLHLWFPAVISGLSLTPAFTLLTWQKIA------PIFAISSIDPSL--I-----------------------------ARIL--PIAGIRA----------LGGI----------------------GGLN----------------------QTDVRLLLSYSS-IAHTGILAR-ITAPAAVLTLYLTIYILINISLFTA-------------------------------------LTQNRVKIHKQLLPGSQKPYESFLLAIT-------------------ILSLGGLPPLTGFAIKLLVLIFTQAKT---I-I-LVSLI------SGAILRLYYYLSLTFS------PLLRLHKIPISQFKLTTSTSISFLLSQ-IMPLSL-------LF 
 
HRGHKIKGTVVLMRKNVLDVNSVTSVGGIIGQGLDLVGSTLDTLTAFLGRSVSLQLISATKADANGKGKLGKATFLEGIITSLPTLGAGQSAFKINFEWDDGSGIPGAFYIKNFMQTEFFLVSLTLEDIPNHGSIHFVCNSWIYNAKLFKSDRIFFANQTYLPSETPAPLVKYREEELHNLRGDGTGERKEWERIYDYDVYNDLGDPDKGENHARPVLGGNDTFPYPRRGRTGRKPTRKDPNSESRSNDVYLPRDEAFGHLKSSDFLTYGLKSVSQNVLPLLQSAFDLNFTPREFDSFDEVHGLYSGGIKLPTDIISKISPLPVLKEIFRTDGEQALKFPPPKVIQVSKSAWMTDEEF----AREMLAGVNPNLIRCLKDFPPR-----SKLDSQVYGDH--TSQITKEHLEPNLEGLTVDEA-IQNKRLFLLDHHDPIMPYLRRINATSTKAYATRTILFLKNDGTLRPLAIELSLPHPQGDQSGAFSQVFL----PADEG-VESSIWLLAKAYVVV-NDSCYHQLVSHWLNTHAVVEPFIIATNRHLSVVHPIYKLLHPHYRDTMNINGLARLSLVNDGGVIEQTFLWGRYSVEMSAVVYKDWVFTDQ-------------------------ALPADLIKRGM--AIEDPSCPHGIR----------LVIEDYPY-TVDG--LEIWDAIKTWVHEYVFLYYKSDDTLREDPEL-------------------QAC-WKELVEVGHG-DK-KN--EP---W-----WPKMQT--REELVEACAIIIWTASALHAAVNFGQYPYGGLILNRPTLSRRFMPEKGSAEYEELRK-NPQKAYLKTITP-------------KFQTLIDLSVIEILSRHASDEVYLGERDNPNWTSDTRALEAFKRFGNKLAQIENKLSERNNDEKLRNRCGPVQMPYTLLLPSSKEGLTFRGIPNSISI------ 
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MKSPHKLLFIFLITTSTV--------IVLSSSNWL----T--TIGL-------------------EINMLGFI---------------------------PLIYHKITANES-----------------------ETVVKYLVPQSLGSTIFIVGALISYHFNSIQILIP-IAIC-LKLGAA-P-------------------LHLWFPAVISGLSLTPAF--TLL--------------------------TWQKIAPIFAISSIDPSLIARILPIAGIRALG----GIGGLNQTDVRLLLSYSSIAHTGILARITA--PAA--VLT------------------------------LYLTIYILINISLFTALTQNR-VKIHKQLLPGSQKPYESFLL-AITILSLGGLPPLTGFAIKLLVLIFTQAKT-IILVSLISGAILRLYYYLSLTFS--------------------PLLRL--------HKIPISQFKLTTSTSISFLLSQIM---------------------------------------------------------------------------------------PLSLLF 
 
TTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIAAIALNPQMPDFNNTILALEQSGELLTRVTS---------------------------------VFFAMTAAHTNDELQRLDEQFSAELAELANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKVLNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWLIPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLLGFPHYAAWKIADQMAKTPEAALN--FM-----------------------------RE-IVPAARQRASDELASIQAVIDKQQGGFSAQPWDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYHPDVRVWEIFDHNGVGLALFYGD-FFA--R------D--SK-SGGAWMGNFVEQSTLNKTHPVIYNVCNYQKPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEF---------------------------PSQINEHWATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEENEAMQDVD--DFEL-RALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGY---YAYL--WTQMLADDGYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKA-PKIMPMLQHRGLNI 
-------------------------------------------------------------------------MKSPHKLLFIFLITTSTVIVLSSSNWLTTIGLEINMLGFIPLIY---H--K-----------------------------------------------------------------------------I-TA-N----------------------------------------------------------------------------------ESETVVKY-LVPQSLGS---T-IF-IVGALISYHF-N-SI--QILIPIAICLKLGAAPLHLWFPAVISGLSLTPAFTLLTWQKIAPIF-AI--S------------SIDPSLIA-RI---------LPIAGIRALGGIGGL---------------------------N--------------------QTDVRLLLSYSSIAHTGILARIT--------------------APAA--------------VLTLYLTIYILI-------------------------NISLFTALTQNRVKIHKQLLPGSQKPYESFLLAITILSLGGLPP-LT----GFAIKLLVLIFTQAKTIILVSLISGAILRLYYYLSLTFSPLLRLHKI---PISQ--FKLTTSTSIS-------------------------FLLS---QIM--P-----------------------------------------------LSLLF---------- 
 
AEMEVQIVRNDPPLRYDTNLPVDLLHMVYAGRGATGSSGVVFGTWYRT--------------------------------------------------IQDRTITDFPLTTRSADFRDGRMSKTFMTALVLSLQACGRLYVGQRHYSAFECAVLCLYLLYRNTHGR--APVTFGDLLGRLPRYLACLAAV--IG-TEG-GRPQYR--YR--D--------D-K--L-----------------PKT--QF-AAGG-GRYEHGALASHIVIATLMHHGVLPAAPGDVP----------V-AH-HDDI-NRAAAAFLSRGHN-LFLWEDQTL---------------------LRA---TANTITALGVIQRLLANGNVYADRLNNRLQLGMLIPGAVSGSDSGAIKSGDNNLEALCANYVLPLYRADPAVELTQLFPGLAALCLDAQAGRRRVVDMSSGARQAALVRLTALELINRTPTPVGEVIHAHDALAIQYEQGLGLLAQQARIGLGSN----------TKRFSAFNVSSDYDMLYFLCLGFIPQYL------ 
------------------------------------------------MKSPHKLLFIFLITTSTVIVLSSSNWLTTIGLEINMLGFIPLIYHKITANESETVVKYLV--P--QSL----GSTIFIVGALISYHFNSIQI-----LIPIAICLKLGAAPLHLWFPAVISGLSLTPAFTLLTWQKIAPI--FAI-SSI-DPSLIARILPIAGIRALGGIGGLNQTDVRLLLSYSSIAHTGILARITAPAAVLTLYLTIYI----------------------------LINISLFTALTQNRVKIH-KQLLPGSQKPYESFLLAITIL---SLGGLPPLTGFAIKLLVLIFTQ--AKTIILVSLISGA----------------------------------------------------------------I--L-RLYYYLSL------------TF------------------------------------------------------SPLLRLHKIPISQFK--LTTSTSISFLLSQIM------PLSLLF 
 
EDIYRKEWKWDKVNWGSHLNICWPQGSCKFYVYVRNGIVWREEQAAQTPACNVDYVDYNPLGCQKGSAFNNNLYGDERVKYPLKRVGKRGEGKWKRVSWDEAAGDIADSIIDSFEAQGS-----------------------------------------------------------DGFILDAPHVHAGSIAWGAGFRMTYLMDGVSPDINVDIGDTYMGAFHTFGK--MHMGYSADNLLDA-ELIFMTCSNWSYTYPSS-------------YHFLSEARYKGAEVVVIAPDFNPTTPAADLHVPVRVGSDAAFWLGLSQVMIDEKLFDRQFVC-EQTDL-PL-LVRMDTGKFLSA-EDVDGGE-AKQFYFFDEKAGSVRKASRGTLKLDFMPALEGTFSARLKNGKTIQVRTVFEGLREHLKDYTPEKASAKCGVPVSLIREL---------------------------------------------------------------GRKVAKKRTC--SY--IG-FSSAKSYHGDLMERSLFLAMALSGNWGKPGTGAFAWAYSDDNMVYLGVMSKPTAQGGMDELHQMAEGFNKRTLEADPTSTDEMGNIEFMKVVTSAVGLVPPAMWLYYHVGYDQLWNNKAWTDPALKKSFGAYLDEAKEKGWWTNDHIRPAPDKTPQVYMLLSQNPMRRKRSGAKMFPDVLFPKLKMIFALETRMSSSAMYADIVLPCAWYYEKHEMTTPCSGNPFFTFVDRSVAPPGECREEWDAIALILKKVGERAAARGLTEFNDHNGRKRRYDELYKKFTMDGHLLTNEDCLKEMVDINRAVGVFAKDYTYEKFKKEGQTRFLSMGTGVSRYAHANEVDVTKPIYPMRWHFDDKKVFPTHTRRAQFYLDHDWYLEAGESLPTHKDTPM-VGGDHPFKI--TG-GHPRVSIHSTHLTNSHLSRLHRGQPVVHMNSKDAAELGIKDGDMAKLFNDFADCEIMVRTAPNVQPKQCIVYFWDAHQYKGWKPYDILLIGMPKPLHLAGGYEQFRYYFMNGSPAPVTDRGVRVSIKKA------ 
-----------------------------------------------------------------------------------------------------------------------MKSPHKLLFIFLITTSTVIVLSSSNWLTTIGLEINMLGFIPLIYHKITANESETVVKYLVPQSLGS-----------------------TIFIVGALISY----------HFNSIQILIPIAICLKLGA--------------APLHLWFPAVISGLSLTPAFT-----LLT--------W--QKIAPIFA--ISSIDPSLIARILP--IAGIRALGGIGGLNQTDVRLLLSYSSIAH--TGI-----LARITAP----------AAVLTLYL-------------------------------------------------------TIYILINISLFTALTQNRVKIHKQLLPGSQKPYESFLLAITILSLGGLPPLTGFAIKLLVLIFTQAKTIILVSLISGAILRLYYYLSLT---------------------------------------------------------------------------------------------F-----------------------------------------------------------------SPLLRLHKI------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PIS--QFKLTTSTSISFLLSQIM-------------------------------------------------------------------------------------------------------------------------PLSLLF 
 
GVYRVCVSTGASIYAGSKNKVELWLVGQHGEVELGSCLRPTRNKEEEFKVNVSKYLGSLLFVRLRKKHFLKEDAWFCNWISVQALGAAEDKYWFPCYRWVVGDGVQSLPVGTGCTTVGDPQGLFQKHREQELEERRKLYQWGSWKEGLILNVAGSKLTDLPVDERFLEDKKIDFEASNSLNILAPWKTLDDFNRIFGRSKLARRVRDSWQEDS---LFG-YQFLNGAN-PML---L------RRSVQLPARLVFPPGMEELQAQLEK-ELKAGTLFEADFALLDNIKANVILYCQQYLAAPLVMLKLQPDGKLMPMVIQLHLPKIGSSPPPLF-----LPTDPPMVWLLAKCWVRSSDFQVHELNSHLLRGHLMAEVFTVATMRCLPSIHPVFKLIVPHLRYTLEINV-RARNGLVSDFG--IFDQIMSTGGGGHVQLLQQAGAFLTYRSF------------------------CP--PDDLADRGL---------L---------------GVESSFYAQDALRL-WEII---SRYVQGIMGLYYKTD---------EA-VRDDLELQSWCREITEIGLQGAQK-QG-FPT--S-LQSVAQA-C--HF-VTM--CIFTCTGQHSSIHLGQLDWFTWVPNAPCTMRLPPPTTKDATLETVMATLPN--------L-KQSSLQMSIVWQLGQPIMVPLGQHQEEYFSGPEPRAVLEKFREELAIMDKEIEVRNEKLDIPYEYLRPSIVENSVAI-------- 
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MKSP--HKLLFIFLITTSTVIVLSSSNWLTTIGL--------------------EIN-MLGFI----------------------------PLIYHKITANE------------------SETVVKYLVPQSLGSTIFIVGALISYHFNSIQI-LIPIAICLKLGA--A--------------PLHLWFPAVISGLSLTPAFTL---LTW----------------------------QKIAPIFAISSIDPSLIARILPIAGIRALG-------GIGGLNQTDVRLLLSYSSIAHTGILARITAPAAVLTL--YL---TIYI--------------LINISLFTAL-TQNRVK-IHKQLLPG-SQKPYESF-LLAITI-LSLGGLPPLTGFAIKLLVLI-FTQAKTIILVSLISGAILRLYYYLSLTFSP-------------L--LRL----HKIPISQFKLTTSTSISFLLSQ-----------------------------------------------------------------IMPLSLLF 
 
(a)Residue pairs that are structurally aligned by TM-align program are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade.(more about the colors used)
(b)Ranking of proteins is based on TM-score of the structural alignment between Model1 and the PDB structures in our template library.
(c)RMSDa is the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.

  Function Prediction

  Predicted EC Numbers

RankTM-scoreRMSDaIDENaCov.EC-ScorePDB
Hit		EC No.
10.3198 7.25 0.10 0.610.25902c17A 1.14.99.9
 
20.3396 6.51 0.08 0.570.22841tebA 2.7.7.48
 
30.3432 6.41 0.08 0.580.22161e1kA 1.18.1.2
 
40.3899 6.55 0.06 0.660.22093bneA 1.13.11.12
 
50.4025 6.23 0.06 0.640.21701n8qA 1.13.11.12
 
(a)Ranking is based on EC-score, which is a confidence score for the Enzyme Classification (EC) Number prediction.
(b)RMSDa is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)IDENa is percentage sequence identity in the structurally aligned region.
(d)Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)EC-Score is defined based on the C-score of the structure prediction and similarity of the model with known enzyme structures, as identified using both global and local structural alignment programs. The global similarity score uses TM-score, IDENa,RMSDa and Cov. of the structural alignment by TM-align, while the local match compares the structural and chemical similarity of local spatial motifs in the model with known catalytic site of enzymes. A prediction with a EC-score >1.1 signifies a prediction with high confidence (upto 3 digit numbers of EC) and vice versa (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).

  Predicted GO terms

RankTMscoreRMSDaIDENaCov.PDB
Hit		Fh-ScoreAssociated GO Terms
10.7495 3.09 0.09 0.863dh4D 0.43 GO:0005351 GO:0005402 GO:0015293 GO:0022892 GO:0031402 GO:0043169 GO:0051119 GO:0006814 GO:0008643 GO:0030001 GO:0005623 GO:0005886 GO:0016021
20.3000 7.19 0.11 0.552cb1A 0.26 GO:0005496 GO:0016765 GO:0030170 GO:0048037 GO:0006520 GO:0009308 GO:0009987 GO:0019752
30.3711 5.63 0.09 0.553detB 0.24 GO:0005247 GO:0005254 GO:0008509 GO:0015297 GO:0022832 GO:0022838 GO:0022892 GO:0031404 GO:0006821 GO:0006950 GO:0005623 GO:0005886 GO:0016021
40.3945 6.59 0.07 0.671rrlB 0.23 GO:0005506 GO:0009055 GO:0016165 GO:0043169 GO:0051213 GO:0009058 GO:0009987 GO:0031407 GO:0031408 GO:0032787 GO:0044249 GO:0046394 GO:0055114 GO:0005622 GO:0005623 GO:0005737
50.3368 5.72 0.09 0.522qi9B 0.23 GO:0015235 GO:0015889 GO:0005623 GO:0005886 GO:0016021
60.3506 6.67 0.08 0.611i5pA 0.23 GO:0005102 GO:0006952 GO:0009405 GO:0009987 GO:0030154 GO:0030435
70.3452 6.44 0.08 0.581e6eC 0.23 GO:0004324 GO:0005515 GO:0009055 GO:0050660 GO:0050661 GO:0006066 GO:0006091 GO:0006694 GO:0006810 GO:0008203 GO:0009058 GO:0009987 GO:0022900 GO:0044249 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0031974 GO:0043229 GO:0044429 GO:0044444
80.2836 6.49 0.11 0.486cscB 0.23 GO:0004108 GO:0006084 GO:0006099 GO:0009056 GO:0009987 GO:0044248 GO:0044262 GO:0046356 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0031974 GO:0043229 GO:0044429 GO:0044444
90.2572 6.48 0.12 0.432o7vA 0.22 GO:0004091 GO:0008152
100.3899 6.55 0.06 0.663bneA 0.22 GO:0005506 GO:0009055 GO:0016165 GO:0043169 GO:0051213 GO:0009058 GO:0009987 GO:0031407 GO:0031408 GO:0032787 GO:0044249 GO:0046394 GO:0055114 GO:0005622 GO:0005623 GO:0005737

Consensus Prediction of Gene Ontology terms 
Molecular Function  Biological Process Cellular Location
GO termGO-Score  GO termGO-Score GO termGO-Score
GO:00054880.184  GO:00099870.140  GO:00056230.246
GO:00038240.139  GO:00081520.139  GO:00444640.246
GO:00431670.112  GO:00442370.117  GO:00160200.133
GO:00052150.090  GO:00442380.117  GO:00312240.133
GO:00431690.089  GO:00512340.113  GO:00160210.133
GO:00468720.089  GO:00068100.113  GO:00444250.133
GO:00090550.068  GO:00511790.113  GO:00057370.112
GO:00164910.068  GO:00060820.071  GO:00444240.112
GO:00228910.067  GO:00197520.071  GO:00058860.112
GO:00152910.067  GO:00442550.068  GO:00056220.112

(a)Ranking in the first table is based on a function prediction score (Fh-score), which is calculated based on the C-score of the structure prediction and the TM-score, IDENa, RMSDa and Cov. of the structural alignment by TM-align between the predicted model and the PDB structures (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).
(b)RMSDa is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)IDENa is the percentage sequence identity in the structurally aligned region.
(d)Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)A consensus prediction of GO terms is derived from the structural analogs that have an Fh-score of >=1.0. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on the Fh-score of the template from which the GO term is derived. A prediction with a GO-score >0.5 signifies a prediction with high confidence and vice versa.


  Predicted Binding Site


Binding site residues in the model:
LYS:2  SER:3  PRO:4  HIS:5  LYS:6  LEU:7  LEU:8  
PHE:9  ILE:10  LEU:137  ALA:139  ARG:140  ILE:141  LEU:142  
PRO:143  ILE:144  ILE:147  ILE:179  THR:180  ALA:183  
Identified analogs with similar binding site:

RankPDB
Hit		TM-scoreRMSDaIDENaCov.BS-scoreDownload
Complex		Binding site residues on the predicted model
13bsnA0.3452 6.89 0.06 0.60 0.93Download2,3,4,5,6,7,8,9,10,137,139,140,141,142,143,144,147,179,180,183
21lox_0.3849 6.60 0.05 0.64 0.25Download81,82,84,85,86,88,89,115,116,117,119,120,124,270,274,306,307,308,309,310,311,312,313,314
31e6eA0.3440 6.34 0.08 0.56 0.18Download10,11,12,13,14,15,16,17,18,19,20,21,55,79,80,191,265,269,273,276,280
43bk2A0.3626 6.27 0.06 0.58 0.16Download14,15,16,17,18,19,20,21,22,23,87,88,91,187,191,226,229,230,262,266
52ivfA0.3877 6.29 0.05 0.62 0.00Download73,74,76,77,78,274,277,278,281,298,299,302,303,306,309,310
62ivfA0.3877 6.29 0.05 0.62 0.00Download76,77,78,81,82,85,124,127,128,131,270,271,274,303,305,306,307,308,309,310,311,312,313,314

(a)Ranking of the analogs in the table is based on their BS-score. BS-score is calculated by first finding the structural analogs of the predicted model based on their TM-score, IDENa and Cov. of the structural alignment. The ligand(s) in the analog structure are then transferred onto the model and the fitness of the ligand-model complex (BS-score) is calculated by comparing the local structure and sequence similarity in the binding site region. (For detail, see Kucukural, Szilagyi, Ambrish, and Zhang, Template based ligand binding site prediction on modeled protein structure, 2009, in preparation).
(b) A BS-score of >0.5 signifies a binding site prediction with high confidence and vice-versa.
(c)RMSDa the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.
(f)The image shows the ligand-protein complex with the best BS-score. The ligand is depicted in magenta colored ball & stick, the predicted binding site residues interacting with the ligand are shown as transparent green spheres, while the N & C terminus in the model are marked by blue and red spheres respectively.



 Please cite following articles when you use the I-TASSER server:
1.Yang Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9:40 (2008).
2.Yang Zhang. Template-based modeling and free modeling by I-TASSER in CASP7. Proteins, 8: 108-117 (2007).
3.Sitao Wu, Jeffrey Skolnick, Yang Zhang. Ab initio modeling of small proteins by iterative TASSER simulations. BMC Biology, 5:17 (2007).