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I-TASSER results for job id S35004

  Submitted Sequence

>120423
MFLLDAAVPVIAFFLLFNFLYLYSPIESMESGWIFKTCDACLQSTCHTENKRPCYTRWNV
DYNFTCFVCPPEFGNDQYFYEDECLKGCTGEKKHCVCDFWCYMCVAKEGFDKANFTQCIV
DPNEEINCK

  Predicted Secondary Structure

Sequence                  20                  40                  60                  80                 100                 120
                   |                   |                   |                   |                   |                   |          
MFLLDAAVPVIAFFLLFNFLYLYSPIESMESGWIFKTCDACLQSTCHTENKRPCYTRWNVDYNFTCFVCPPEFGNDQYFYEDECLKGCTGEKKHCVCDFWCYMCVAKEGFDKANFTQCIVDPNEEINCK
PredictionCCCHCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCSSSSCCCCCCSSSSCCCCCCCCCSSCHHHHHCCCCCCCCCSSSHHHHHHHHHHHCCCCCCCCSSSCCCCHCCCCC
Conf.Score911201018999999999999728255532461066768988766135778873256524544078877844488641503356435767765166324555545452256236405642883202779

  Top 5 Models predicted by I-TASSER

Download Model 1 Download Model 2 Download Model 3 Download Model 4 Download Model 5
C-score=-4.10
 C-score=-4.14
 C-score=-4.27
 C-score=-4.30
 C-score=-4.71
 
Estimated accuracy of Model1: 0.28±0.09 (TM-score)    14.1±3.8Å (RMSD)    (Read more about C-score of generated models)

  Top 10 templates used by I-TASSER

RankPDB
Hit		Iden1Iden2Cov.Norm.
Z-score		Download
Align.		                   20                  40                  60                  80                 100                 120
                   |                   |                   |                   |                   |                   |         
Sec.Str
Seq		CCCHCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCSSSSCCCCCCSSSSCCCCCCCCCSSCHHHHHCCCCCCCCCSSSHHHHHHHHHHHCCCCCCCCSSSCCCCHCCCCC
MFLLDAAVPVIAFFLLFNFLYLYSPIESMESGWIFKTCDACLQSTCHTENKRPCYTRWNVDYNFTCFVCPPEFGNDQYFYEDECLKGCTGEKKHCVCDFWCYMCVAKEGFDKANFTQCIVDPNEEINCK
11b5qA 0.16 0.23 0.99 0.73Download GGVYDEDYVQKRIELADSVEEMGEKLSATASGRDDMSILAMQRLNEHQPNGPATPVDMVVDYYKFDFAEPPRVTSLQNTVPLATFSDFGDDVYFVADQYEAVVYYLAGQYLKTD-SGKIVDPRLQLNKV
21hcfA 0.26 0.19 0.71 0.73Download --GVSETAPAS-------RRGELAVCDA-VSGWVT--------------DRR-----TAVDLRGREVEVLGEVGGRQYFFETRCKAGCRGVDR----RHWVSECKAKQSYVRALT----ADAQGRVGC-
31danL 0.25 0.25 0.87 0.68Download -----------AAFLLCSFARIFKDARTKLFWISYSDGDQCASSPCQ--NGGSCKDQLQ---SYICF-CLPAFGRNCETHKDDQLICVNGCEQYCSDHTGKRSCRCHEGYDGVSCTPTVEYPCGKIPIL
41bunA 0.22 0.16 0.71 0.97Download N--------------LINFMEMEKTWGEYASGRPIDALDRCCYVNCYGDKKHKCNPKTQ-SYSYKLT-------KRTII--------CYGAAGTCVCD--CALCFGNSEYIEGHK-----NIDTARFCQ
51pwoA 0.30 0.25 0.78 0.81Download ------------NLYQFRKMIKCEPLLAFTSGTPVDELDRCCQTHDN------CYDKAEKLPECKGILSGPY--VNTYSY--DCTDGCNDQKDKCNCDRTAAMCFAKAPYIEANNH---IDPN---RCK
61e9tA 0.23 0.09 0.17 0.97Download -----------------------------------------------------------------------------------------------------------EEYVGLSANQCAVPAKDRVDCG
71b77A 0.16 0.20 0.84 0.66Download MKLSKDTIAILKNFASINSGILLSQ------GKFIMTRAVNGTTYAEANISDEIDFDVAL-YDLNSFLSISLVSDDAEISMHTDGNIKIADTR------------STVYWPAAD-KSTIVFPNKPIQFP
82uwoA 0.14 0.10 0.22 1.01Download ---------------------------------------------------------------------------------KLCSLDNGDCDQFCHEEQNSVVCSCARGY-------------------
91s5jA 0.13 0.15 0.92 0.40Download YNFRDAEITLFNNDLTMKLIVLFSGIEELTRTEISTWVKNLYYWEHRKRNWLPLKEEILAKSS-NAVVIDPP----AGIFFNITFASLYP---SIIRTWLSYETVDIQQCKKPYE--VKDETGEVLHIV
101rutX 0.15 0.20 0.81 0.69Download GKIADRFL-LYA-----------------MDSYWHSRCLKCSSCQAQLDIGTSSYTKSGMILCRNDYISGACSACGQSIPASELVMRAQGNVYHLKC-FTCSTC--RNRLVPG---DRFHYINGSLFCE
(a)All the residues are colored in black; however, those residues in template which are identical to the residue in the query sequence are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade. (more about the colors used)
(b)Rank of templates represents the top ten threading templates used by I-TASSER.
(c)Ident1 is the percentage sequence identity of the templates in the threading aligned region with the query sequence.
(d)Ident2 is the percentage sequence identity of the whole template chains with query sequence.
(e)Cov. represents the coverage of the threading alignment and is equal to the number of aligned residues divided by the length of query protein.
(f)Norm. Z-score is the normalized Z-score of the threading alignments. Alignment with a Normalized Z-score >1 mean a good alignment and vice versa.
(g)Download Align. provides the 3D structure of the aligned regions of the threading templates.
(h)The top 10 alignments reported above (in order of their ranking) are from the following threading programs:
       1: MUSTER   2: HHSEARCH   3: SP3   4: PROSPECT2   5: PPA-I   6: HHSEARCH I   7: FUGUE   8: HHSEARCH II   9: SPARKS   10: MUSTER   

  10 proteins in PDB which are structurally closest to the first I-TASSER model (identified by TM-align)

RankTM-scoreRMSDaIDENaCov.PDB
Hit
10.4797 5.04 0.05 0.872ckfA
Model1
 
20.4701 4.18 0.07 0.782uvaG
Model1
 
30.4615 4.96 0.03 0.821llqA
Model1
 
40.4568 4.49 0.04 0.772ftwA
Model1
 
50.4538 4.93 0.05 0.821mjgA
Model1
 
60.4531 4.26 0.05 0.751gkpA
Model1
 
70.4502 4.77 0.04 0.791kcxA
Model1
 
80.4498 4.17 0.06 0.742ftyA
Model1
 
90.4480 4.74 0.02 0.791gklA
Model1
 
100.4470 4.51 0.05 0.781gq2A
Model1
 
Structural alignment using TM-align
 
MSGDTTLVDTVNAS-QSRQ-VFWDRDV-YDLEI-ERIFSRAHDPEAPSLEDYLGEFRFYLDTIWEGGGAGLE-LLGP-PMKSLLHCNWKVP-VENFVGDGYHVGWTHAAALGQIGGPLAGLAGNRADDL--GLQFTTRHGHGFGVIDNAAAAIHRKGDGWNKYLEDTRGEVRRKFGADRERLYVGHWNGAIFPNCSFLYGTNTFKIWHPRGPHEIEVWTYTMVPSDADPATKSAIQREATRTFGTAGTLESDDGENMSS-ATYVNRGVITRDGM--MNSTMGVGYEGPHPVYPGIVGISFIGETSYRGFYRFWKEMIDAPDWASVKANDD-NWDSVFTNRNFWNEKLNA---- 
---MF-LLD----AAVPVIAFF-L--LFNF-LYLYSPIESMES-GWIFKTCDACLQS-TC---H--------TE---NK------------RP--CYT-------------------------------RWNVDYN-----F----------------------------------------------TCF--V------------CPP----EFGNDQY-----------------------------F--Y--EDE-CLK------------GCTGE-K---------------------K-HCVCDFWCYMCVAKE----GFDKANFTQCIVDPNEE---------INCK 
 
QSLRPLVLTHGSLEFSFLVPTSLHFQAAQLKDSFLATLPQPTEELAQDDEPSSVVELVARYIAFVAHEVDEGDEDAHPTNLEVLKLILNEFERAFMRGNDVHAIAANVAGITAKKIGVVRAYYAGRAAAGRAPKPYDSALFRAAAENNVKIYSIFG-G--QGN--IEEYFDELR-E-IYTTYPSFVE-DLITSIA-EL---------LQSLAREWDAVKQYPKGLDILQWLHN-P-E-S-QPDTDYLVS---A--PVSFPLIGLVQLAH-YMITCKTLGREPGELLERFSGTTGHSQGIVVAAAIATARTWDEFATAAKRAVELLFWIGLRSQQAYPRTSLAPSTLQDSVENGEGTPTPMLSIRDLTRSAVQEHIDATNQHLPEDRHIGISLVNSARNFVVTGPPISLYGLNLRLRKVKAPTGLDQNRIPFTQRKARFVNRFLPITAPFHSPYLAGAHAHILGDVDDMKIPASSLVIPVYDTKTGQDLRELGDEDIIPELVRMITYDPVNWETATVFPDATHIVDFGPGGVSGIGVLTNRNKDGTGVRVILAGAIDGTNTEVGYKPELFDRDDNAVQFAVDWVKEHGPRLVKTSVGQTFVDTKMSRLLGVPPVMVAGMTPTTVPWDFVAATMNAGYHIELAGGGYYNAQKMSDAISKIEKAIPPGRGITVNLIYVNPRAMGWQIPLLGRLRADGVPIEGLTIGAGVPSIEVANEYIQTLGIRHISFKPGSVDAIQQVINIAKANPTFPIILQWTGGRGGGHHSFEDFHQPILLMYSRIRKCSNIVLVAGSGFGGSEDTYPYLTGSWSTKFGYPPMPFDGCMFGSRMMTAKEAHTSKQAKQAIVDAPGVDDDQWENTYKRPTGGVITVLSEMGEPIHKLATRGVLFWKELDDKIFSLDRSKRVAELKKRRDYIIKKLNDDFQKVWFGRNSAGEPVDLEDMTYAEVVHRMVELMYVKHEKRWIDPSLKKLTGDFIRRVEERFTSVEGQPSLLQNYSDLDEPYPAVDRILAAYPEASTQLINAQDVQHFLLLCQRRGQKPVPFVPALDENFEYWFKKDSLWQSEDIEAVYGQDVGRTCILQGPVAAKYSKVIDEPIKDILDGIHNDHIKFLLRDLYDGKEENVPVIEYFGGRILKATDEEPDIDGLTASRDANKISYRLSNAPSANLPDVDSFMQLIAGNSYSWRHAMFTTEVFVQGHRFQTNPLKRLFAPTRGMYVEITNPDDPAKTVISVREPSQSAKLVKTVEIKLVGDNEIALTLFEGRTAEGGVVPLTFRFTYHPEAGYAPIREVMEGRNDRIKEFYYRVWFAEKEVPFDTPLTAVFDGGREIVNAQAVADFVHAVGNTGEAFVDRGKDFFAPMDFAIVVGWKAITKPIFPRKIDGDLLKLVHLSNGYRMVPGAEPLKVGDVLDTTAQINAVINQDSGKMVEVCGTLKRDGKPVMYVTSQFLYRGVYTDYENTFQRKDEVPMQLHIATPQDLAVLRSKEWFKLDDQHDIELLGQTLVFRLQSLVRFKNKNVYSSVQTIGQVLLELPTKEIIQVASVDYEAGESHGNPVIDYLQRHGSSIEQPVNFENPIPLSGKTPLELRAPASNENYARVSGDYNPIHVSRVFSSYANLPGTITHGMYTSAAVRSLVETWAAENNIGRVRSYHVNMVGMVLPNDAITVKLEHVGMIAGRKIIKVDARNKDTDESVLQGEAEVEQPVTAYVFTGQGSQEQGMGMDLYATSPVAKEVWDRADKHFRENYGFSIIDIVKNNPKELTVHFGGPRGKIIRQNYMSMTFETVNADGSIKTEKIFKEVDENSTSYTYRSPSGLLSATQFTQPALTLMEKASFEDMRSKGLVQRDSTFAGHSLGEYSALVALADVMPIESLVSVVFYRGLTMQVAVERDEQGRSNYAMCAVNPSRISPTFTEQALQYVVENIAEVTGWLLEIVNYNVANMQYVAAGDLRALDTLANVLNILKMQKIDIQALMQSMSLEDVRAHLVEIIQECRKQTEAKPQPVQLERGFATIPLRGIDVPFHSTFLRSGVKPFRSFLLKKINKTTIDPSKLIGKYIPNVTAKPFEISKEYFEEVHRLTGSPKIANILANWDKYE--- 
---------------------------------------------------------------------------------------------------------------------------------------------------------MFLLDAAVPVIAFFLLFNFLYLYSPIESMESGWIFKTCDACLQSTCHTENKRPCYTRW--NVDYN--F--TCFV--CPP-EFGNDQYFYEDECLKGCTGEKKHCV-CDFWCYMCVAKEGF--------D----------------------------K-A--NF-TQC--IV--D------------------------------------------------------------------------------------------------------------------------------------PN--E-------------------------------E--I----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NCK 
 
SVAHHEDVYSHNLPPMDEKEMALYKLYRPERVTPKKRSAELLKEPRLNKGMGFSLYERQYLGLHGLLPPAFMTQEQQAYRVITKLREQPNDLARYIQLDGLQDRNEKLFYRVVCDHVKELMPIVYTPTVG-LACQNFGY-IY-R--K--PK-GLYITINDNSVSK----IYQILSNWHEEDVRAIVVTDGERILGLGDLGAYG-IGIPVG--KLALYVALGGVQPKWCLPVLLDVGTNNMDLLNDPFYIGLRHKRVRGKDYDTLLDNFMKACTKKYGQKTLIQFEDFANPNAFRLLDKYQDKYTMFNDDIQGTASVIVAGLLTCTRVTKKLVSQEKYLFFGAGAASTGIAEMIVHQMQNEGISKEEACNRIYLMDIDGLVTKNRKEMNPRHVQFAKDMPETTSILEVIRAARPGALIGASTVRGAFNEEVIRAMAEINERPIIFALSNPTSKAECTAEEAYTFTNGAALYASGSPFPNFELNGHTYKPGQGNNAYIFPGVALGTILFQIRHVDNDLFLLAAKKVASCVTEDSLKVGRVYPQLKEIREISIQIAVEMAKYCYKNGTANLYPQPEDLEKYVRAQVYN----------------------TEY-EE-LINATYDWPEQDMRHGFPVPVVRH---- 
----------------------------------------------------------------------------------------------------MF------LLD-AAVPVIAFFLL-F-NFLYLYSPIESMESGWIFKTCDACLQSTCHTE-------NKRPCY-TR--W-NVDYNFTCFV-C--PPEFGN-D---QY-FY--EDECLKGC-TGEKKH--CVCD------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FWCYMCVAKEGFDKANFTQCIVD--PN-EE-----------------------INCK 
 
TGTILIKNGTVVNDDRYFKSDVLVENGIIKEISKNIEPKEGIKVVDATDKL--LLPGGIDTHTHFQLPFMGTVSVDDFDIGTQAAVAGGTT-FI--IDFVIPTRGQSLLEAYDQWKKWADEKVNCDYSLHVAITWWSEQVSREMEILVKERGVNSFCFMAYKNSFMVTDQEMYHIFKRCKELGAIAQVHAENGDMVFEGQKKMLEMGITGPEGHELSRPEALEAEATNRAIVIADSVCTPV-YIVHVQSIGAADVICKHRKEGVRVYG-EPIAAGLGVDGSHMWNHDWRHAAAFVM--GPPIRPDPRTKGVLMDYLARGDLDCVGTDN--CTFCADQKAMGKDDFTKIPNGV-NGVEDRMSIVWENGVNTGKLTWCQFVR-ATSSERARIFNIYPRKGRIDVGCDGDIVIWDPNQSKTISKDTHHHAVDFNIFEGIKVTGIAVTTIVAGNIVWSDNKLSCVKGSGRFVPRPPFGPVFDGIEQRDKVRNELLRKVDR--------------- 
------------MF-------------------------------------LLDAAVP---------------------------VI-AFFLLFNFLY----------------L-------------YSPIE---------------------SME---------------------S----G-W----------------------------------------------------IFKTCD---------------------ACLQSTC------------------------HTENKRP----CYTRW-NVDYN--F-TCFVCPPEFG-----------------NDQYFYEDECLKGCTG-EK----K----HC-VCDFWCYMCVA-KE-------------------------------------------------------------------------------G-FDKAN----------------FTQCIVDPNEEINCK 
 
RFRDL-S-HNC--RPSEAP-RVMEPKNRDRTVDPAVLEMLVKSKDDKVITAFDRFVAQQPQCKIGYEGICCRFCMAGPCRIKATDGPGSRGICGASAWTIVARNVGLMILTGAAAHCEHGNHIAHALVEMAEGKAPDYSVKDEAKLKEVCRRVGIEVEGKSVLELAQEVGEKALEDFRRLKGEGEATWLMTTINEGRKEKFRTHNVVPFGIHASISELVNQAHMGMDNDPVNLVFSAIRVALADYTGEHIATDFSDILFGTPQPVVSEAKNRVVAGWSLEALTKLLATQNAQNPIRVLNQAILDGELAGVALICGCN-NLKGFQDNSHLTVMKELLKNN-V-F-VVATGCSAQAAGKLGLLDPANVETYCGDGLKGFLKRLGEGANIEIGLPPVFHMGSCVDNSRAVDLLMAMANDLGVDTPKVPFVASAPEAMSGKAAAIGTWWVSLGVPTHVGTMPPVEGSDLIYSILTQIASDVYGGYFIFEMDPQV-AARKIL-DA---------LEYRTWKLGVHKEVAERYETKLCQGY--- 
-MFL-LDAAVPVIAFFLL-FNF-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LY--LY--SPIESMESG-WIFKTCDACLQST----------------------------------------------CHTENKR-PC--Y--TR-WN----VDYN-F-TCFVCPPEFG-NDQYFYEDEC-----L----------------KGCTG-EK-KHCVCDF------WCY-MCVAK-EGFDKANFTQCIVDPNEEI----------------NCK 
 
PLLIKNGEIITADSRYKADIYAEGETITRIGQNLEAPPGTEVIDATGKYVFPGFIDPHVHIYLPFMATFAKDTHETGSKAALMGGTTTYIEMCCPSRNDDALEGYQLWKSKAEGNSYCDYTFHMAVSKFDEKTEGQLREIVADGISSFIFLSYKNFFGVDDGEMYQTLRLAKELGVIVTAHCENAELVGRLQQKLLSEGKTGP-EWH-EPSRPE-A-VEAEGTARFATFLETTGATGY--------VV--HLSCKPALDAAMAAKARGVPIYIESV-IP-------------H---------FLLDKTYAERG--GVEAMKYIMSPPLRDKRNQKVLWDALAQGFIDTVGTDHCPFDTEQKLLGKEA------------------FTAIPNGIPAIEDRVNLLYTYGVSRGRLDIHRFVDAASTKAAKLFGLFPRKGTIAVGSDADLVVYDPQYRGTISVKTQHVNNDYNGFEGFEIDGRPSVVTVRGKVAVRDGQFVGEKGWGKLLRREPMYF------ 
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------M-FL--LD-AAVPV-IAFFLLFNFLYLYSPIESMESGW------------IFKTCDACLQSTCH---------------------TENKRPCYTRWNVDYNFTCFVCPPEFGNDQYFYEDECLKGCTGE-KKHCVC-DFW---------------------------------------CYMCVAKEGFDKANFTQCIVDPN----------------------------------------------------------------------------------------------------------------------------EEINCK 
 
DRLLIRGGRIINDDQSFYADVYLEDGLIKQIGENLIVPGGVKTIEANGRMVIPGGIDVNTYLQKPSQGMTSADDFFQGTKAALAGGTTMIIDHVVPEPGSSLLTSFEKWHEAADTKSCCDYSLHVDITSWYDGVREELEVLVQDKGVNSFQVYMAYKDLYQMSDSQLYEAFTFLKGLGAVILVHAENGDLIAQEQKRILEMGITGPEGHALSRPEELEAEAV-FRAIAIAGRINCPVYITKVMSKSAADIIALARKKGPLVF--G-EP--IAA-SL-GTDGTHYWSKNWAKAAAFV----------TSPPLS---PDPTTPDYLTSLLACGDLQVTGSGHCPYSTAQKAV----GKD--NF-TLIPEGVNGIEERMTVVWDKAVATGKMDENQFVAVTSTNAAK------------------IFNLYPRKGRIAVGSDADVVIWDPDKMKTITAKSHKSTVEYNIFEGMECHGSPLVVISQGKIVFEDGNISVSKGMGRFIPRKPFPEHLYQRVRIRSKVFG------------- 
-------------------------------------------------------------------------------------------------------------------------------------------------------MFLLDAA-----------------------------VP--VIAF----------------FLLFNF-LY--LY---------------------SPIESMESGW----IFKTCDACLQSTCHTENKRPC----------------YTRWNVDYNFT-CFVCPPEFGNDQY-----------------------------FYEDE--CLKGCTGEKKHC-----------------------------------VCDFWCYMCVAKEGFDKANFT-------------------------------------------------------------------------------------------------QCIVDPNEEINCK 
 
PIYDLIIKNGIICTASDIYAAEIAVNNGKVQLIAASIDPSLGSEVIDAEGAFITPGGIDAHVHVDEPLKLLGDVVDTMEHATRSAVAGGTTTVVAFSTQDVSKKGPSALAESVKLDVDEYSEQTLYCDYGLHLILFQIEKPSVEARELLDVQLQAAYNDYGVSSVMFMTYPGLQISDYDIMSAMYATRKNGFTTMLHAENGDMVKWMIEALEEQGLTDAYYHGVSRPSIVEGEATNRAITLATTMDTPILFVHVSSPQAAEVIKQAQTKGLKVYAETCPQYALLSDAITRCHGVGIDLSSISESPFTNPD-DRF--IGSKYICSPPIRPEGTQKSIWKGMNNGTFT-------------------------------------IVGSDHCS-YNYYEKTS-TASKHRAFDPENNKNGEFRYIP--N-------GLPGV-CTRMPLLYDYGYLRGNLTSMMKLVEIQCTNPAKVYGMYPQKGSILPGVSDADLVIWYPDDSKKEYNSKPKLITNKLMEHNCDYTPFEGIEIKNWPRYTIVKGKIVYKEGEILKENADGKYLKRGKSFMCTPKNEWVTEWRPKYE--------------- 
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MF--LLDAAVPVIA-----------FF--LL-FN--FLY-----------------------------LYSPIESMESGWIFKTCDACLQSTCHTENKRPCYTRWNVDYNFTCFVCPPEFGNDQYFY--E----------D--ECLKGCTGEKKHCVCD-FWC---------------------------------------------------------------------------------YMCVAKEGFDKAN------------------------------------------------FTQCIVDPNEEINCK 
 
SFK-Y--ESA--VQ--Y-RPAPDSYLNPCPQAGRIVKETYTGINGTKSLNVYLPYGYDPNKKYNIFYLMHGGGENENTIFSNDVKLQNILDHAIMNGELEPLI-VV-TPTFNGGNCTAQNFYQEFRQNVIPFVESKYSTYAESTTPQGIAASRMHRGFGGFAMGGLTTWYVMVNCLDY-VAYFMPLSGDYWYGNSPQDKANSIAEAINRSGLSKREYFVFAATGSEDIAYANMNPQIEAMKALPHFDYTSDFSKGNFYFLVAPGATHWWGYVRHYIYDAL-PY-------------FFHELEHHHHHH-- 
MFLLDAAVPVIAFFLLFNFLYLY-SP----------------------------------------------------I----ESMESGWI---------FKTCDACLQ-----------------------------------------------STCHT--ENKR-P-CY-TRWNVDYNFTCFVC-----------------------------------PPE-FGNDQYFY--ED--E----CLKGCTG-EKKHCV---------------CD-FWCYMCVAKEGFDKANFTQCIVDPNEEIN--CK 
 
KKGYEVLRDPHLNKGAFTLEERQQLNIHGLLPPCFLGQDAQVYSILKNFERLTSDLDRYILLSLQDRNEKLFYKVLTSDIERF-PIVYTPTVG-LAC-QHYG-LAFR-RP--RG--LFITIHDRGH----IATLQSWPESVIKAIVVTDGERILGL-GDL-GCYGG--IPVG--KLALYTACGGVKPHQCLPVLDVGTDNETLLKDPLYIGLRHKRIRGQAYDDLLDEFEAVTSRYGNCLIQFEDFANANAFRLLHKYRNKYCTFNDDIQGTASVAVAGLLAALRITKNRLSDHTVLFQGAGEAALGIANLIVAQKEGVSKEEAIKRIWVDSKGLIVKGRASLTPEKEHFAHEHCEKNLEDIVKDIKPTVLIGVAAIGGAFTQQILQDAAFNKRPIIFALSNPTSKAECTAEQLYKYTEGRGIFASGSPFDPVTLPSGQTLYPGQGNNSYVFPGVALGVISCGLKHIGDDVFLTTAEVIAQEVSEENLQEGRLYPPLVTIQQVSLKIAVRIAKEAYRNNTASTYPQPEDLEAFIRSQVYSTDY------------------------NCFVADSYTWPEEAKVK------ 
---------------------------------------------------------------MF-----LLDAAVP--VIAFFLL-FN-FLYLYSPIESMESGWIFKTCDACLQSTCHTE-----NKRPCY-TR-WNVDYNFTCFVC----PPE-FG--ND----QYFY--EDECLKGCT--GEKKH-CVCD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FWCYMCVAKEGFDKANFTQCIVDPN----------------EEINCK 
 
(a)Residue pairs that are structurally aligned by TM-align program are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade.(more about the colors used)
(b)Ranking of proteins is based on TM-score of the structural alignment between Model1 and the PDB structures in our template library.
(c)RMSDa is the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.

  Function Prediction

  Predicted EC Numbers

RankTM-scoreRMSDaIDENaCov.EC-ScorePDB
Hit		EC No.
10.4664 4.30 0.10 0.780.53252pffE 2.3.1.86
 
20.4665 4.28 0.10 0.780.53222pffB 2.3.1.86
 
30.3796 4.55 0.14 0.650.52201o60D 2.5.1.55
 
40.4060 4.76 0.11 0.730.50672nxiJ 2.5.1.55
 
50.4409 4.71 0.09 0.780.49761te8A 2.7.7.48
 
(a)Ranking is based on EC-score, which is a confidence score for the Enzyme Classification (EC) Number prediction.
(b)RMSDa is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)IDENa is percentage sequence identity in the structurally aligned region.
(d)Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)EC-Score is defined based on the C-score of the structure prediction and similarity of the model with known enzyme structures, as identified using both global and local structural alignment programs. The global similarity score uses TM-score, IDENa,RMSDa and Cov. of the structural alignment by TM-align, while the local match compares the structural and chemical similarity of local spatial motifs in the model with known catalytic site of enzymes. A prediction with a EC-score >1.1 signifies a prediction with high confidence (upto 3 digit numbers of EC) and vice versa (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).

  Predicted GO terms

RankTMscoreRMSDaIDENaCov.PDB
Hit		Fh-ScoreAssociated GO Terms
10.3796 4.55 0.14 0.651o60D 0.34 GO:0008676 GO:0000271 GO:0008653 GO:0009058 GO:0009987 GO:0019294 GO:0033692 GO:0034637 GO:0034960 GO:0034961 GO:0043283 GO:0043284 GO:0044249 GO:0046394 GO:0046400 GO:0005622 GO:0005623 GO:0005737
20.3598 4.85 0.13 0.651zh7B 0.32 GO:0003700 GO:0003707 GO:0008270 GO:0008289 GO:0043169 GO:0043565 GO:0006139 GO:0006629 GO:0009058 GO:0009889 GO:0042127 GO:0043283 GO:0044249 GO:0044260 GO:0048518 GO:0048522 GO:0060255 GO:0065008 GO:0005622 GO:0005623 GO:0005634 GO:0043229 GO:0044424
30.4108 4.72 0.11 0.732oqoA 0.32 GO:0008233 GO:0008658 GO:0016763 GO:0009058 GO:0009653 GO:0016043 GO:0042221 GO:0044085 GO:0044238 GO:0048856 GO:0048869 GO:0050793 GO:0050794 GO:0065008 GO:0005623 GO:0005886 GO:0009274 GO:0016021
40.4349 4.72 0.11 0.741i84V 0.32 GO:0003774 GO:0003779 GO:0005516 GO:0005524 GO:0008307 GO:0030554 GO:0006461 GO:0006939 GO:0006941 GO:0007507 GO:0007517 GO:0010926 GO:0010927 GO:0022607 GO:0030029 GO:0030241 GO:0031032 GO:0031034 GO:0048251 GO:0048468 GO:0048646 GO:0048739 GO:0048856 GO:0055001 GO:0070271 GO:0005622 GO:0005623 GO:0005737 GO:0005863 GO:0015629 GO:0030016 GO:0030017 GO:0032982 GO:0043229 GO:0043234
50.3846 4.77 0.12 0.701rq1A 0.32 GO:0005515 GO:0009055 GO:0016671 GO:0016972 GO:0050660 GO:0050662 GO:0006091 GO:0006457 GO:0006467 GO:0006810 GO:0009987 GO:0022900 GO:0034960 GO:0043412 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0012505 GO:0016020 GO:0042175 GO:0043229 GO:0044432 GO:0044444
60.3966 5.08 0.11 0.732fymF 0.32 GO:0000287 GO:0004634 GO:0042802 GO:0006006 GO:0006066 GO:0006091 GO:0006096 GO:0009056 GO:0009987 GO:0016052 GO:0044260 GO:0044265 GO:0044275 GO:0046164 GO:0000015 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005856 GO:0009986 GO:0043229 GO:0044445
70.3551 5.38 0.12 0.701vz5B 0.31 GO:0009055 GO:0016491
80.3498 5.57 0.11 0.712ys7A 0.30 GO:0004637 GO:0005524 GO:0030554 GO:0006144 GO:0006725 GO:0009058 GO:0009113 GO:0009987 GO:0044249 GO:0046148 GO:0046483
90.3905 4.02 0.13 0.601u90A 0.30 GO:0005525 GO:0019001 GO:0007154 GO:0007264 GO:0065007 GO:0005622 GO:0005623 GO:0005886
100.3621 5.18 0.12 0.661x9dA 0.30 GO:0004571 GO:0005509 GO:0043169 GO:0006487 GO:0009058 GO:0009100 GO:0009101 GO:0009311 GO:0009987 GO:0034960 GO:0043412 GO:0043413 GO:0044249 GO:0005622 GO:0005623 GO:0005624 GO:0005737 GO:0005783 GO:0005789 GO:0012505 GO:0016020 GO:0016021 GO:0042175 GO:0043229 GO:0044432 GO:0044444

Consensus Prediction of Gene Ontology terms 
Molecular Function  Biological Process Cellular Location
GO termGO-Score  GO termGO-Score GO termGO-Score
GO:00038240.252  GO:00099870.313  GO:00056230.282
GO:00054880.250  GO:00081520.251  GO:00444640.282
GO:00001660.124  GO:00442380.251  GO:00056220.251
GO:00170760.124  GO:00442600.221  GO:00444240.220
GO:00055150.095  GO:00442370.219  GO:00057370.188
GO:00305540.094  GO:00431700.189  GO:00432290.186
GO:00167870.094  GO:00090580.158  GO:00432260.186
GO:00431670.094  GO:00432830.157  GO:00432270.155
GO:00468720.094  GO:00442490.126  GO:00432310.155
GO:00325530.093  GO:00349600.125  GO:00444440.125

(a)Ranking in the first table is based on a function prediction score (Fh-score), which is calculated based on the C-score of the structure prediction and the TM-score, IDENa, RMSDa and Cov. of the structural alignment by TM-align between the predicted model and the PDB structures (For details, see Ambrish, Kucukural and Zhang,Large scale benchmarking of protein function prediction using predicted protein structures, 2009, submitted).
(b)RMSDa is the RMSD between models and the PDB structure in the structurally aligned regions by TM-align.
(c)IDENa is the percentage sequence identity in the structurally aligned region.
(d)Cov. represents the coverage of the alignment and is equal to the number of structurally aligned residues divided by length of model.
(e)A consensus prediction of GO terms is derived from the structural analogs that have an Fh-score of >=1.0. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on the Fh-score of the template from which the GO term is derived. A prediction with a GO-score >0.5 signifies a prediction with high confidence and vice versa.


  Predicted Binding Site


Binding site residues in the model:
LEU:4  PHE:13  PHE:17  LEU:20  PRO:25  ILE:26  MET:29  
LEU:42  VAL:68  TYR:80  LEU:85  CYS:88  VAL:96  ASP:98  
PHE:99  TRP:100  CYS:101  TYR:102  MET:103  
Identified analogs with similar binding site:

RankPDB
Hit		TM-scoreRMSDaIDENaCov.BS-scoreDownload
Complex		Binding site residues on the predicted model
11zx0C0.3822 4.96 0.08 0.72 0.19Download4,13,17,20,25,26,29,42,68,80,85,88,96,98,99,100,101,102,103
21f3lA0.4110 4.86 0.07 0.72 0.19Download4,13,17,20,26,29,38,39,42,68,85,88,96,97,98,99,100,101,102,103,105
31svwA0.4174 4.25 0.09 0.68 0.19Download25,26,27,29,30,31,32,33,39,42,43,48,50,70,102
42ex4A0.4171 5.07 0.08 0.76 0.18Download13,17,25,26,41,42,43,45,46,66,67,68,69,70,80,81,84,85,96,97,98,99,100,101,102
52erbA0.3283 4.15 0.14 0.51 0.17Download4,10,13,14,16,17,18,19,20,21,22,25,69,70,71,72,73,74,75,80,82,85,86,89,96,99,100
62zfuA0.4000 4.97 0.11 0.74 0.17Download3,4,5,13,17,20,25,26,42,68,80,85,88,89,96,97,98,99,100,101,102,103
71el4A0.3777 5.19 0.09 0.68 0.17Download2,4,13,17,20,25,38,85,98,99,100,101,102,103,104,105,106,110,118,119,120,128
81z5xE0.3717 4.56 0.10 0.62 0.16Download42,44,45,46,47,48,50,53,54,55,56,63,64,65,66,67,68,78,84,93,94,95,96,97,98,100
91t2eA0.3425 5.41 0.09 0.64 0.15Download12,13,14,15,16,17,18,19,20,21,22,23,24,25,50,51,52,68,69,70,71,72,73,75,76,77,78,79,80,99
101np7A0.3900 5.07 0.08 0.72 0.14Download53,55,63,64,65,66,67,78,79,80,81,84,93,94,95,96,97,100

(a)Ranking of the analogs in the table is based on their BS-score. BS-score is calculated by first finding the structural analogs of the predicted model based on their TM-score, IDENa and Cov. of the structural alignment. The ligand(s) in the analog structure are then transferred onto the model and the fitness of the ligand-model complex (BS-score) is calculated by comparing the local structure and sequence similarity in the binding site region. (For detail, see Kucukural, Szilagyi, Ambrish, and Zhang, Template based ligand binding site prediction on modeled protein structure, 2009, in preparation).
(b) A BS-score of >0.5 signifies a binding site prediction with high confidence and vice-versa.
(c)RMSDa the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the model.
(f)The image shows the ligand-protein complex with the best BS-score. The ligand is depicted in magenta colored ball & stick, the predicted binding site residues interacting with the ligand are shown as transparent green spheres, while the N & C terminus in the model are marked by blue and red spheres respectively.



 Please cite following articles when you use the I-TASSER server:
1.Yang Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9:40 (2008).
2.Yang Zhang. Template-based modeling and free modeling by I-TASSER in CASP7. Proteins, 8: 108-117 (2007).
3.Sitao Wu, Jeffrey Skolnick, Yang Zhang. Ab initio modeling of small proteins by iterative TASSER simulations. BMC Biology, 5:17 (2007).